pCMV-To-NRXN2(human)-Myc-6×His-Zeo
$128.00
In stock
SKU
PL34525
NM_015080.4
100μg liquid plasmid(Instantly):$149
| name | pCMV-To-NRXN2(human)-Myc-6×His-Zeo | another name | apCMV-To-NRXN2(human)-Myc-6×His-Zeo |
| Insert Size (bp) | 5136bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Zeo | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGAACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CCCTATCAGT GATAGAGATC TCCCTATCAG TGATAGAGAT CGTCGACGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC ACGCTGTTTT GACCTCCATA GAAGACACCG GGACCGATCC AGCCTCCGGA CTCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC CGCCACCATG GCGTCCGGGA GCCGGTGGCG GCCGACACCG CCGCCGCTGC TGTTGCTGCT GCTGCTGGCG CTGGCGGCGC GCGCGGACGG CCTGGAGTTC GGCGGCGGCC CCGGGCAGTG GGCTCGCTAC GCGCGCTGGG CGGGCGCGGC GAGCAGCGGC GAGCTCAGCT TCAGCCTGCG CACCAACGCC ACGCGCGCGC TGCTGCTCTA CCTGGACGAC GGCGGCGACT GCGACTTCCT GGAGCTGCTG CTGGTGGACG GCCGCCTGCG GCTGCGCTTC ACGCTTTCGT GCGCCGAGCC GGCCACGCTG CAGCTGGACA CGCCGGTGGC CGACGACCGC TGGCACATGG TGCTGCTGAC CCGCGACGCG CGCCGCACGG CGCTGGCGGT GGACGGCGAG GCCCGCGCCG CCGAGGTGCG CTCCAAGCGG CGCGAGATGC AGGTGGCCAG CGACCTGTTC GTGGGCGGCA TCCCGCCCGA CGTGCGCCTC TCGGCGCTTA CGCTGAGCAC CGTCAAGTAC GAGCCGCCCT TCCGCGGCCT CTTGGCCAAC CTGAAGCTGG GCGAGCGGCC CCCCGCGCTG CTGGGCAGCC AGGGCCTGCG CGGCGCCACC GCCGACCCGC TGTGCGCGCC CGCGCGCAAC CCCTGCGCCA ACGGCGGCCT CTGCACCGTG CTGGCCCCCG GCGAGGTGGG CTGCGACTGC AGCCACACGG GCTTCGGCGG CAAGTTCTGC AGCGAAGAGG AGCACCCCAT GGAAGGTCCG GCTCACCTGA CGTTAAACAG CGAAGTAGGG TCCTTACTGT TCTCCGAGGG GGGGGCCGGG AGAGGAGGAG CCGGCGATGT GCACCAGCCA ACAAAAGGCA AGGAGGAGTT TGTGGCGACC TTCAAAGGCA ATGAGTTCTT CTGCTACGAC CTGTCACACA ACCCCATCCA GAGCAGCACT GATGAGATCA CACTGGCCTT CCGCACCCTG CAACGCAACG GCCTGATGCT GCATACAGGC AAGTCGGCCG ACTACGTCAA CCTGTCCCTC AAGTCTGGGG CTGTCTGGCT GGTCATCAAC CTAGGCTCAG GTGCCTTCGA GGCCCTTGTG GAACCCGTCA ATGGCAAGTT CAACGACAAC GCCTGGCACG ACGTCCGGGT CACCCGAAAC CTGCGCCAGC ACGCAGGGAT TGGACACGCT ATGGTAAACA AACTGCATTA TCTGGTGACC ATCTCGGTGG ACGGGATCCT GACCACCACA GGCTACACGC AGGAGGATTA CACCATGCTG GGCTCTGATG ACTTCTTCTA CATTGGGGGC AGCCCCAACA CAGCTGACCT GCCGGGCTCG CCCGTCAGCA ACAACTTCAT GGGCTGCCTC AAGGACGTGG TCTATAAGAA CAATGACTTC AAATTGGAAC TATCCCGCCT GGCAAAGGAA GGGGACCCCA AGATGAAGCT GCAGGGGGAC TTGTCATTCC GCTGTGAGGA TGTGGCTGCC CTGGACCCTG TGACCTTTGA GAGTCCCGAG GCCTTTGTGG CGCTGCCCCG CTGGAGCGCT AAGCGCACTG GCTCCATCTC CCTAGACTTC CGCACCACCG AGCCCAATGG GCTGCTGCTC TTCAGCCAGG GCCGGCGGGC TGGGGGTGGA GCTGGCAGCC ACAGCTCTGC TCAGCGGGCC GACTACTTTG CCATGGAGCT ATTGGACGGC CACCTCTATC TTCTGCTGGA CATGGGATCT GGGGGCATCA AGCTGCGGGC ATCCAGCCGC AAGGTCAATG ATGGCGAGTG GTGTCACGTG GACTTCCAGA GGGATGGGCG AAAAGGCTCC ATCTCAGTGA ATAGTCGCAG CACGCCGTTC TTGGCCACTG GAGACAGCGA GATTCTGGAC CTGGAGAGTG AGCTGTACCT GGGCGGTCTC CCTGAGGGGG GCCGGGTGGA CCTGCCCCTG CCCCCAGAGG TGTGGACAGC AGCACTCCGG GCAGGCTACG TGGGCTGTGT GCGGGACCTC TTCATAGATG GGCGTAGCCG AGACCTCCGG GGCCTGGCTG AGGCTCAGGG GGCTGTGGGC GTTGCCCCCT TTTGCTCCCG GGAGACGCTG AAGCAGTGTG CATCTGCCCC CTGTCGCAAT GGGGGCGTCT GTCGAGAAGG CTGGAACCGC TTCATCTGTG ACTGCATCGG GACCGGCTTT CTTGGGCGGG TCTGTGAGAG AGAGGCCACG GTCCTGAGCT ACGATGGCTC CATGTACATG AAGATCATGC TGCCTAACGC CATGCACACG GAGGCAGAGG ATGTGTCCCT GCGTTTCATG TCCCAGCGGG CCTACGGACT CATGATGGCC ACCACTTCCA GGGAGTCTGC CGACACCCTA CGCCTGGAGC TGGATGGGGG GCAGATGAAG CTCACTGTCA ACCTCGACTG CCTGCGCGTC GGCTGCGCAC CCAGTAAAGG CCCCGAAACG CTGTTTGCGG GGCACAAGCT CAATGACAAT GAGTGGCACA CGGTGAGGGT GGTCCGGCGT GGCAAGAGCC TGCAGCTGTC TGTGGACAAC GTGACTGTGG AGGGACAGAT GGCAGGAGCC CATATGCGGC TGGAGTTCCA CAACATTGAG ACGGGCATCA TGACGGAGCG GCGGTTTATC TCCGTGGTGC CCTCCAACTT CATCGGGCAT CTGAGTGGGC TCGTGTTCAA TGGCCAGCCC TACATGGACC AGTGCAAGGA TGGTGACATC ACCTACTGTG AGCTCAATGC TCGCTTTGGC CTGCGTGCCA TCGTGGCCGA TCCCGTCACC TTCAAGAGTC GCAGCAGCTA CCTGGCACTC GCCACGCTCC AAGCCTATGC TTCCATGCAC CTCTTCTTCC AGTTCAAGAC CACGGCCCCT GATGGGCTTC TTCTGTTCAA CTCGGGCAAC GGCAATGACT TCATTGTCAT CGAGCTGGTC AAGGGGTACA TCCACTACGT GTTTGACCTG GGGAATGGCC CGTCCTTGAT GAAGGGGAAC TCAGACAAAC CAGTCAATGA CAACCAGTGG CACAACGTGG TGGTGTCCAG GGACCCAGGC AACGTGCACA CGCTCAAGAT TGACTCCCGC ACTGTCACGC AGCACTCCAA TGGCGCCCGA AACCTCGATC TCAAAGGGGA GTTGTACATT GGCGGTCTGA GCAAGAATAT GTTCAGCAAC CTGCCCAAGC TGGTGGCCTC CCGGGATGGC TTTCAGGGCT GCCTGGCCTC AGTGGACCTC AACGGACGTC TCCCAGACCT CATCGCCGAC GCCCTGCACC GCATTGGGCA GGTGGAGAGG GGCTGTGATG GCCCCAGCAC CACCTGCACT GAAGAGTCCT GTGCCAACCA GGGCGTCTGC TTGCAGCAGT GGGATGGCTT CACCTGCGAC TGCACCATGA CTTCCTATGG AGGCCCTGTC TGCAATGATC CCGGGACCAC ATACATCTTT GGGAAGGGGG GAGCGCTCAT CACCTACACG TGGCCCCCCA ATGACAGGCC CAGCACGAGG ATGGATCGCC TGGCCGTGGG CTTCAGCACC CACCAGCGGA GCGCTGTGCT GGTGCGGGTG GACAGCGCCT CCGGCCTTGG AGACTACCTG CAGCTGCACA TCGACCAGGG CACCGTGGGG GTGATCTTTA ACGTGGGCAC GGACGACATT ACCATCGACG AGCCCAACGC CATAGTAAGC GACGGCAAAT ACCACGTGGT GCGCTTCACT CGAAGCGGCG GCAACGCCAC CCTGCAGGTG GACAGCTGGC CGGTCAACGA GCGGTACCCG GCAGGAAACT TTGATAACGA GCGCCTGGCG ATTGCTAGAC AGAGAATCCC CTACCGGCTT GGTCGAGTAG TAGATGAGTG GCTGCTCGAC AAAGGCCGCC AGCTGACCAT CTTCAACAGC CAGGCTGCCA TCAAGATCGG GGGCCGGGAT CAGGGCCGCC CCTTCCAGGG CCAGGTGTCC GGCCTCTACT ACAATGGGCT CAAGGTGCTG GCGCTGGCCG CCGAGAGCGA CCCCAATGTG CGGACTGAGG GTCACCTGCG CCTGGTGGGG GAGGGGCCGT CCGTGCTGCT CAGTGCGGAG ACCACGGCCA CCACCCTGCT GGCTGACATG GCCACCACCA TCATGGAGAC TACCACCACC ATGGCCACTA CCACCACGCG CCGGGGCCGC TCCCCCACAC TGAGGGACAG CACCACCCAG AACACAGATG ACCTGCTGGT GGCCTCTGCT GAGTGTCCAA GCGATGATGA GGACCTGGAG GAGTGTGAGC CCAGTACTGG AGGAGAGTTA ATATTGCCCA TTATCACGGA GGACTCCTTA GACCCCCCTC CCGTGGCCAC CCGATCCCCC TTCGTGCCCC CGCCCCCTAC CTTCTACCCC TTCCTCACGG GAGTGGGCGC CACCCAAGAC ACGCTGCCCC CGCCCGCCGC GCGCCGCCCG CCCTCTGGGG GCCCGTGCCA GGCCGAGCGG GACGACAGCG ACTGCGAGGA GCCCATCGAG GCCTCGGGCT TCGCCTCCGG GGAGGTCTTT GACTCCAGCC TCCCCCCCAC GGACGACGAG GACTTTTACA CCACCTTTCC CCTGGTCACG GACCGCACCA CCCTCCTGTC ACCCCGCAAA CCCGCTCCCC GGCCCAACCT CAGGACAGAT GGGGCCACGG GCGCCCCTGG GGTGCTGTTT GCCCCCTCCG CCCCGGCCCC CAACCTGCCG GCGGGCAAAA TGAACCACCG AGACCCGCTT CAGCCCTTGC TGGAGAACCC GCCCTTGGGG CCCGGGGCCC CCACGTCCTT TGAGCCGCGG AGGCCCCCTC CCCTGCGCCC CGGCGTGACC TCAGCCCCCG GCTTCCCCCA TCTGCCCACA GCCAACCCCA CAGGGCCTGG GGAGCGGGGC CCGCCGGGCG CAGTGGAGGT GATCCGGGAG TCCAGCAGCA CCACGGGCAT GGTGGTGGGC ATTGTGGCGG CGGCGGCGCT CTGCATCCTC ATCCTCCTCT ACGCCATGTA TAAGTACCGC AATCGTGATG AGGGCTCCTA CCAGGTGGAC CAGAGCCGAA ACTACATCAG TAACTCGGCC CAGAGCAATG GGGCGGTGGT GAAAGAGAAG GCCCCGGCTG CCCCCAAGAC GCCCAGCAAG GCCAAGAAGA ACAAAGACAA GGAGTATTAT GTCCTCGAGT CTAGAGGGCC CGCGGTTCGA ACAAAAACTC ATCTCAGAAG AGGATCTGAA TATGCATACC GGTCATCATC ACCATCACCA TTGAGTTTAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG AACCAGCTGG GGCTCTAGGG GGTATCCCCA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGCATC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG GGGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTAA TTCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CTGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAGCACGTG TTGACAATTA ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG GTGAGGAACT AAACCATGGC CAAGTTGACC AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC GTCGCCGGAG CGGTCGAGTT CTGGACCGAC CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG GACGACTTCG CCGGTGTGGT CCGGGACGAC GTGACCCTGT TCATCAGCGC GGTCCAGGAC CAGGTGGTGC CGGACAACAC CCTGGCCTGG GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC GCCGAGTGGT CGGAGGTCGT GTCCACGAAC TTCCGGGACG CCTCCGGGCC GGCCATGACC GAGATCGGCG AGCAGCCGTG GGGGCGGGAG TTCGCCCTGC GCGACCCGGC CGGCAACTGC GTGCACTTCG TGGCCGAGGA GCAGGACTGA CACGTGCTAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCAATGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTC
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