pAAV-Strn(rat)-EGFP
$84.00
In stock
SKU
PL34501
NM_019148.3;striatin, calmodulin binding protein
100μg liquid plasmid(Instantly):$105
| name | pAAV-Strn(rat)-EGFP | another name | apAAV-Strn(rat)-EGFP |
| Insert Size (bp) | 2340bp | Plasmid host | Mammary cell,Adeno-associated virus |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Rat | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT CTACGTAGCC ATGCTCTAGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGAGCTTGGC CCATTGCATA CGTTGTATCC ATATCATAAT ATGTACATTT ATATTGGCTC ATGTCCAACA TTACCGCCAT GTTGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC ATGGTGATGC GGTTTTGGCA GTACATCAAT GGGCGTGGAT AGCGGTTTGA CTCACGGGGA TTTCCAAGTC TCCACCCCAT TGACGTCAAT GGGAGTTTGT TTTGGCACCA AAATCAACGG GACTTTCCAA AATGTCGTAA CAACTCCGCC CCATTGACGC AAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG CAGAGCTCGT TTAGTGAACC GTCAGATCGC CTGGAGACGC CATCCACGCT GTTTTGACCT CCATAGAAGA CACCGGGACC GATCCAGCCT CCCCTCGAAG CTGATCCTGA GAACTTCAGG GTGAGTCTAT GGGACCCTTG ATGTTTTCTT TCCCCTTCTT TTCTATGGTT AAGTTCATGT CATAGGAAGG GGAGAAGTAA CAGGGTACAC ATATTGACCA AATCAGGGTA ATTTTGCATT TGTAATTTTA AAAAATGCTT TCTTCTTTTA ATATACTTTT TTGTTTATCT TATTTCTAAT ACTTTCCCTA ATCTCTTTCT TTCAGGGCAA TAATGATACA ATGTATCATG CCTCTTTGCA CCATTCTAAA GAATAACAGT GATAATTTCT GGGTTAAGGC AATAGCAATA TTTCTGCATA TAAATATTTC TGCATATAAA TTGTAACTGA TGTAAGAGGT TTCATATTGC TAATAGCAGC TACAATCCAG CTACCATTCT GCTTTTATTT TATGGTTGGG ATAAGGCTGG ATTATTCTGA GTCCAAGCTA GGCCCTTTTG CTAATCATGT TCATACCTCT TATCTTCCTC CCACAGCTCC TGGGCAACGT GCTGGTCTGT GTGCTGGCCC ATCACTTTGG CAAAGAATTC AGCCACCATG GACGAGCAGG CGGGGCCCGG CGTCTTCTTC AGCAACAACC ATCCGGGAGC TGGCGGTGCT AAGGGGCTCG GGCCTCTGGC GGAGGCTGCA GCGGCGGGAG ACGGAGCGGC TGCGGCGGGG GCGGCCCGAG CTCAGTACAG CCTCCCGGGG ATCTTGCACT TCCTGCAGCA CGAATGGGCC CGCTTCGAGG TGGAGAGAGC CCAGTGGGAG GTGGAGCGGG CGGAGTTGCA GGCTCAGATT GCTTTCCTTC AGGGGGAGAG GAAAGGCCAA GAAAACTTGA AGAAAGATCT TGTGAGGAGG ATCAAAATGT TGGAATATGC TCTGAAGCAG GAAAGAGCCA AATACCACAA GTTGAAATAT GGGACAGAAC TGAATCAGGG AGACATGAAG CCTCCAAGCT ATGATTCTGA TGAAGGCAAT GAAACGGAGG TGCAGCCACA GCAGAACAGC CAGTTCATGT GGAAGCAAGG CCGGCAGCTC CTCAGACAGT ATCTCCAGGA GGTCGGCTAC ACAGATACTA TTCTAGATGT GAAGTCCAAG CGTGTGCGGG CATTGTTGGG CTTTTCAAGT GATGTCACTG ACAGGGAGGA TGACAAAAAC CAGGACTCGG TGATAAATGG CACAGAGGCT GAAGTTAAAG AGACAGCAAT GATTGGAAAA TCTGAGTTAA CGGATTCTGC CTCCGTGCTG GACAATTTCA AATTCCTTGA AAATGCAGCT GCAGATTTCA GTGATGAAGA CGAGGATGAG GACATTGATG GAAGAGAGAA AAGTGTCATT GATACTTCAA CCATTGTTAG GAAAAAACCA TTACCTGATA CCAGTGAAGA TCGAGACACA AAAGAAGCTC TGAAGGAGTT TGACTTCCTT GTCGCCTCAG AGGAAGGAGA CAACGAGTCC AGAAGTGCAG GCGACGGAAC GGACTGGGAA AAGGAAGATC AGTGTCTGAC GCCGGAGGCC TGGAATGTGG ACCAGGGAGT GATCACCAGA CTGAAGGAGC AGTACAAAAA GGAGAGGAAG GGGAAGAAAG GGGTGAAGAG GCCCAATAGG TCAAAACTAC AAGATATGCT TGCTAATTTG AGAGACGTTG ATGAACTTCC CTCCTTGCAG CCATCTGTTG GCTCCCCTTC CAGACCCAGC AGCTCCAGGC TTCCTGAGCA GGACATCAGT AGGGCAGATG AAGTGGAAGC ATTGACGTTC CCGCCTTCTT CAGGGAAGTC ATTTATCATG GGAGCAGATG AAGCCCTTGA AAGCGAGCTG GGGCTTGGAG AATTAGCAGG CCTTACAGTG GCCAATGAAG CAGACTCATT AGCTTATGAT ATAGCGAATA ACAAAGACGC CTTGAGGAAG ACGTGGAACC CTAAGTTTAC ATTAAGGAGT CACTTTGATG GCATCCGAGC CCTTGCTTTC CACCCCATCG AGCCTGTGTT AATAACCGCG TCAGAGGACC ACACGCTGAA AATGTGGAAT CTGCAGAAAA CAGCCCCAGC TAAAAAGAGT ACTTCTTTAG ATGTGGAGCC TATCTACACC TTCAGGGCTC ACAAAGGTCC GGTGCTGTGT GTGGTCATGA GTAGCAATGG TGAGCAGTGC TACAGCGGCG GCACTGATGG CCTCATCCAG AGCTGGAGCA CCACCAATCC CAACGTCGAC CCCTACGATT CTTATGATCC TTCGGTTCTA AGAGGCCCTC TGTTGGGCCA CACGGATGCG GTGTGGGGGC TGGCCTACAG CGCGGCCCAC CAGCGCTTGT TGTCCTGTTC AGCAGACGGC ACTCTTCGTC TATGGACCAC CACTGAGGTT GCTCCAGCGC TGACTGTATT TAATGATAAC CAAGAACTGG GAATCCCTGC TTCTGTGGAT CTGGTGAGCA GTGACCCGAG CCATATGGTA GCATCGTTCA GCAAGGGATA TACAAGCATC TTCAACATGG AAACACAGCA GCGAATTCTC ACGTTAGAAT CCAATGTCGA TTCAACGGCC AGTTCTTCCT GCCAAATAAA CAGAGTCATC AGTCACCCCA CCCTCCCGAT CAGCATCACT GCTCACGAGG ACAGGCACAT CAAGTTCTAC GATAACAACA CAGGCAAACT GATCCACTCG ATGGTGGCCC ACCTGGAAGC CGTTACAAGT TTGGCGGTTG ATCCCAACGG CCTGTACCTG ATGTCTGGCA GTCATGACTG TTCAATACGC TTGTGGAATT TAGAAAGTAA GACGTGTATC CAGGAGTTCA CAGCTCATCG GAAAAAGTTT GAAGAGTCGA TCCATGATGT AGCATTCCAC CCTTCTAAAT GCTATATAGC CAGCGCTGGA GCCGACGCCC TGGCTAAGGT CTTTGTAGGT GGAGGCGGAT CAGGAGGCGG TGGATCTACG CGGGCCCGGG ATCCACCGGT CGCCACCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAAAGC GGCCGCTCTA GAGGATCCAA GCTTATCGAT ACCGTCGACC TCGAGGGCCC AGATCTAATT CACCCCACCA GTGCAGGCTG CCTATCAGAA AGTGGTGGCT GGTGTGGCTA ATGCCCTGGC CCACAAGTAT CACTAAGCTC GCTTTCTTGC TGTCCAATTT CTATTAAAGG TTCCTTTGTT CCCTAAGTCC AACTACTAAA CTGGGGGATA TTATGAAGGG CCTTGAGCAT CTGGATTCTG CCTAATAAAA AACATTTATT TTCATTGCAA TGATGTATTT AAATTATTTC TGAATATTTT ACTAAAAAGG GAATGTGGGA GGTCAGTGCA TTTAAAACAT AAAGAAATGA AGAGCTAGTT CAAACCTTGG GAAAATACAC TATATCTTAA ACTCCATGAA AGAAGGTGAG GCTGCAAACA GCTAATGCAC ATTGGCAACA GCCCCTGATG CCTATGCCTT ATTCATCCCT CAGAAAAGGA TTCAAGTAGA GGCTTGATTT GGAGGTTAAA GTTTTGCTAT GCTGTATTTT ACATTACTTA TTGTTTTAGC TGTCCTCATG AATGTCTTTT CACTACCCAT TTGCTTATCC TGCATCTCTC AGCCTTGACT CCACTCAGTT CTCTTGCTTA GAGATACCAC CTTTCCCCTG AAGTGTTCCT TCCATGTTTT ACGGCGAGAT GGTTTCTCCT CGCCTGGCCA CTCAGCCTTA GTTGTCTCTG TTGTCTTATA GAGGTCTACT TGAAGAAGGA AAAACAGGGG GCATGGTTTG ACTGTCCTGT GAGCCCTTCT TCCCTGCCTC CCCCACTCAC AGTGACCCGG AATCCCTCGA CATCTAGAGC ATGGCTACGT AGATAAGTAG CATGGCGGGT TAATCATTAA CTACAAGGAA CCCCTAGTGA TGGAGTTGGC CACTCCCTCT CTGCGCGCTC GCTCGCTCAC TGAGGCCGGG CGACCAAAGG TCGCCCGACG CCCGGGCTTT GCCCGGGCGG CCTCAGTGAG CGAGCGAGCG CGCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGCGATT CCGTTGCAAT GGCTGGCGGT AATATTGTTC TGGATATTAC CAGCAAGGCC GATAGTTTGA GTTCTTCTAC TCAGGCAAGT GATGTTATTA CTAATCAAAG AAGTATTGCG ACAACGGTTA ATTTGCGTGA TGGACAGACT CTTTTACTCG GTGGCCTCAC TGATTATAAA AACACTTCTC AGGATTCTGG CGTACCGTTC CTGTCTAAAA TCCCTTTAAT CGGCCTCCTG TTTAGCTCCC GCTCTGATTC TAACGAGGAA AGCACGTTAT ACGTGCTCGT CAAAGCAACC ATAGTACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA AAATATTAAC GCTTACAATT TAAATATTTG CTTATACAAT CTTCCTGTTT TTGGGGCTTT TCTGATTATC AACCGGGGTA CATATGATTG ACATGCTAGT TTTACGATTA CCGTTCATCG ATTCTCTTGT TTGCTCCAGA CTCTCAGGCA ATGACCTGAT AGCCTTTGTA GAGACCTCTC AAAAATAGCT ACCCTCTCCG GCATGAATTT ATCAGCTAGA ACGGTTGAAT ATCATATTGA TGGTGATTTG ACTGTCTCCG GCCTTTCTCA CCCGTTTGAA TCTTTACCTA CACATTACTC AGGCATTGCA TTTAAAATAT ATGAGGGTTC TAAAAATTTT TATCCTTGCG TTGAAATAAA GGCTTCTCCC GCAAAAGTAT TACAGGGTCA TAATGTTTTT GGTACAACCG ATTTAGCTTT ATGCTCTGAG GCTTTATTGC TTAATTTTGC TAATTCTTTG CCTTGCCTGT ATGATTTATT GGATGTTGGA ATCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCT
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