pEX18-Amp
$99.00
In stock
SKU
PL34408
100μg liquid plasmid(Instantly):$120
| name | pEX18-Amp | another name | apEX18-Amp |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene editing | Fragment type | |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | SacB | Fluorescent labeling | |
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATAATCG GCATTTTCTT TTGCGTTTTT ATTTGTTAAC TGTTAATTGT CCTTGTTCAA GGATGCTGTC TTTGACAACA GATGTTTTCT TGCCTTTGAT GTTCAGCAGG AAGCTAGGCG CAAACGTTGA TTGTTTGTCT GCGTAGAATC CTCTGTTTGT CATATAGCTT GTAATCACGA CATTGTTTCC TTTCGCTTGA GGTACAGCGA AGTGTGAGTA AGTAAAGGTT ACATCGTTAG GATCAAGATC CATTTTTAAC ACAAGGCCAG TTTTGTTCAG CGGCTTGTAT GGGCCAGTTA AAGAATTAGA AACATAACCA AGCATGTAAA TATCGTTAGA CGTAATGCCG TCAATCGTCA TTTTTGATCC GCGGGAGTCA GTGAACAGAT ACCATTTGCC GTTCATTTTA AAGACGTTCG CGCGTTCAAT TTCATCTGTT ACTGTGTTAG ATGCAATCAG CGGTTTCATC ACTTTTTTCA GTGTGTAATC ATCGTTTAGC TCAATCATAC CGAGAGCGCC GTTTGCTAAC TCAGCCGTGC GTTTTTTATC GCTTTGCAGA AGTTTTTGAC TTTCTTGACG GAAGAATGAT GTGCTTTTGC CATAGTATGC TTTGTTAAAT AAAGATTCTT CGCCTTGGTA GCCATCTTCA GTTCCAGTGT TTGCTTCAAA TACTAAGTAT TTGTGGCCTT TATCTTCTAC GTAGTGAGGA TCTCTCAGCG TATGGTTGTC GCCTGAGCTG TAGTTGCCTT CATCGATGAA CTGCTGTACA TTTTGATACG TTTTTCCGTC ACCGTCAAAG ATTGATTTAT AATCCTCTAC ACCGTTGATG TTCAAAGAGC TGTCTGATGC TGATACGTTA ACTTGTGCAG TTGTCAGTGT TTGTTTGCCG TAATGTTTAC CGGAGAAATC AGTGTAGAAT AAACGGATTT TTCCGTCAGA TGTAAATGTG GCTGAACCTG ACCATTCTTG TGTTTGGTCT TTTAGGATAG AATCATTTGC ATCGAATTTG TCGCTGTCTT TAAAGACGCG GCCAGCGTTT TTCCAGCTGT CAATAGAAGT TTCGCCGACT TTTTGATAGA ACATGTAAAT CGATGTGTCA TCCGCATTTT TAGGATCTCC GGCTAATGCA AAGACGATGT GGTAGCCGTG ATAGTTTGCG ACAGTGCCGT CAGCGTTTTG TAATGGCCAG CTGTCCCAAA CGTCCAGGCC TTTTGCAGAA GAGATATTTT TAATTGTGGA CGAATCGAAC TCAGGAACTT GATATTTTTC ATTTTTTTGC TGTTCAGGGA TTTGCAGCAT ATCATGGCGT GTAATATGGG AAATGCCGTA TGTTTCCTTA TATGGCTTTT GGTTCGTTTC TTTCGCAAAC GCTTGAGTTG CGCCTCCTGC CAGCAGTGCG GTAGTAAAGG TTAATACTGT TGCTTGTTTT GCAAACTTTT TGATGTTCAT CGTTCATGTC TCCTTTTTTA TGTACTGTGT TAGCGGTCTG CTTCTTCCAG CCCTCCTGTT TGAAGATGGC AAGTTAGTTA CGCACAATAA AAAAAGACCT AAAATATGTA AGGGGTGACG CCAAAGTATA CACTTTGCCC TTTACACATT TTAGGTCTTG CCTGCTTTAT CAGTAACAAA CCCGCGCGAT TTACTTTTCG ACCTCATTCT ATTAGACTCT CGTTTGGATT GCAACTGGTC TATTTTCCTC TTTTGTTTGA TAGAAAATCA TAAAAGGATT TGCAGACTAC GGGCCTAAAG AACTAAAAAA TCTATCTGTT TCTTTTCATT CTCTGTATTT TTTATAGTTT CTGTTGCATG GGCATAAAGT TGCCTTTTTA ATCACAATTC AGAAAATATC ATAATATCTC ATTTCACTAA ATAATAGTGA ACGGCAGGTA TATGTGATGG GTTAAAAAGG ATCGATCCTC TAGCTAGAGT CGATCTTCGC CAGCAGGGCG AGGATCGTGG CATCACCGAA CCGCGCCGTG CGCGGGTCGT CGGTGAGCCA GAGTTTCAGC AGGCCGCCCA GGCGGCCCAG GTCGCCATTG ATGCGGGCCA GCTCGCGGAC GTGCTCATAG TCCACGACGC CCGTGATTTT GTAGCCCTGG CCGACGGCCA GCAGGTAGGC CGACAGGCTC ATGCCGGCCG CCGCCGCCTT TTCCTCAATC GCTCTTCGTT CGTCTGGAAG GCAGTACACC TTGATAGGTG GGCTGCCCTT CCTGGTTGGC TTGGTTTCAT CAGCCATCCG CTTGCCCTCA TCTGTTACGC CGGCGGTAGC CGGCCAGCCT CGCAGAGCAG GATTCCCGTT GAGCACCGCC AGGTGCGAAT AAGGGACAGT GAAGAAGGAA CACCCGCTCG CGGGTGGGCC TACTTCACCT ATCCTGCCCG GCTGACGCCG TTGGATACAC CAAGGAAAGT CTACACGAAC CCTTTGGCAA AATCCTGTAT ATCGTGCGAA AAAGGATGGA TATACCGAAA AAATCGCTAT AATGACCCCG AAGCAGGGTT ATGCAGCGGA AAAGCGCTGC TTCCCTGCTG TTTTGTGGAA TATCTACCGA CTGGAAACAG GCAAATGCAG GAAATTACTG AACTGAGGGG ACAGGCGAGA GACGATGCCA AAGAGCTACA CCGACGAGCT GGCCGAGTGG GTTGAATCCC GCGCGGCCAA GAAGCGCCGG CGTGATGAGG CTGCGGTTGC GTTCCTGGCG GTGAGGGCGG ATGTCGATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GCATATTTGA ATGTATTTAG AAAAATAAAC AAAAAGAGTT TGTAGAAACG CAAAAAGGCC ATCCGTCAGG ATGGCCTTCT GCTTAATTTG ATGCCTGGCA GTTTATGGCG GGCGTCCTGC CCGCCACCCT CCGGGCCGTT GCTTCGCAAC GTTCAAATCC GCTCCCGGCG GATTTGTCCT ACTCAGGAGA GCGTTCACCG ACAAACAACA GATAAAACGA AAGGCCCAGT CTTTCGACTG AGCCTTTCGT TTTATTTGAT GCCTGGCAGT TCCCTACTCT CGCATGGGGA GACCCCACAC TACCATCGGC GCTACGGCGT TTCACTTCTG AGTTCGGCAT GGGGTCAGGT GGGACCACCG CGCTACTGCC GCCAGGCAAA TTCTGTTTTA TCAGACCGCT TCTGCGTTCT GATTTAATCT GTATCAGGCT GAAAATCTTC TCTCATCCGC CAAAACAGCC AAGCTCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGTA CCGAGCTCGA ATTCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAATCACTA GTGCGGCCGG GAAGCCGATC TCGGCTTGAA CGAATTGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTC
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