NM_001359875.1;2600010B19Rik; 3110050K21Rik;4930570G11Rik
100μg liquid plasmid(Instantly):$149
| name | pCMV-Zc3h13(mouse)-3×FLAG-Neo | another name | apCMV-Zc3h13(mouse)-3×FLAG-Neo |
| Insert Size (bp) | 5190bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAC TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTTGGTACC GAGCTCGGAT CCACTAGTAA CGGCCGCCAG TGTGCTGGAA TTCGCCACCA TGTCAAAAAT TAGGAGAAAG GTCACAGTGG AAAACACCAA GACCATATCT GAGAGTACAT CCCGAAGACC TAGCGTATTT GAGAGGCTTG GCCCCAGCAC TGGAAGCACA ACAGAGACAC AGTGTCGTAA CTGGCTGAAG ACTGGCAGCT GCCTCTATGG AAACACATGT AGGTTCATAC ATGGCCCTTC ACCTCGTGGG AAAGGCTACA GCAGCAATTA CAGAAGGTCA CCAGAAAGAC CCACAGGTGA CCTCAGAGAA AGAATGAAGA ATAAGCGTCA GGATGTGGAT TCTGAATCCC AGAAACGGAA TACAGAGGAG CCATCCTCAC CTGTTAGGAA AGAATCTTCA AGAGGGAGAC ATAGAGACAA GGAAGACATA AAAATTGTTA AGGAGAGAAC TCCAGAAAGT GAAGAAGAAA ATGTAGAATG GGAAACTAAC AGAGATGATT CTGACAATGG AGATATTAAT TATGATTATG TTCATGAGTT ATCATTGGAA ATGAAGCGGC AGAAAATACA GAGAGAATTG ATGAAATTGG AACAAGAAAA TATGGATAAG AGAGAAGAAA TTATCATTCA AAAGGAGGTT TCCCCAGAAG TGGTTAGATC AAAATTGTCT CCATCACCTT CTCTGAGAAA GTCCAGTAAA TCTCCAAAAC GAAAGTCCAG CCCCAAGGCA TCTTCAGCTG GCAAGAAGGA GCGGAAGGCT GCTGTGGTGG CTTCTCCGCT GTTGGACCAG CAGAGAAATT CAAAGGGCAA CCAAAGTAAA AAGAAAGGGC CGCGCACTCC GAGTCCACCT CCTCCTATAC TAGAAGATAT CATTCTGGGG AAAAAGTACA AAGAAAAGTA TAAAGTGAAA GACAGGATAG AAGAAAAACC AAGAGATGGG AAGGACAGAG GCCGGGATTT TGAAAAGCAA AGAGAAAAAC GAGACAAGCC AAGGTCTTCT TCTCCGGGAC AGCACCATTC TCCTCTGTCT TCTAGACATC ACTCGTCATC CTCACAGTCA GGGTCATCTA TTCAGAGACA CTCTCCTTCT CCTCGCCGGA AGAGAACTCC TTCCCCATCC TACCAGAGGA CTTTAACTCC ATCTTTACGA CGTTCTGCCT CTCCTTATCC AACCCACTGT CTGTCTTCTC CTCAGAGAAA GCAAAGTCCT CCAAGACATC GCTCTCCAAT GCGAGAGAAA GGGAGGCATG ATCACGAACG GACTTCACAG TCACATGACC GGCGTCACGA AAGGAGGGAA GAAACTCGAG GCAAAAGGGA TAGAGAAAAG GATACCAGAG AAGAGCGAGA GTCTGAACAT GACCACAGGG ATGACAGAGA ACCACGAGAC AGCCGGGACC GGAGAGACAC TAGAGACCGC AGGGAACTTA GAGACTCCAG AGACATGCGG GACTCCAGAG AGATGAGGGA TTATAGCAGA GATGCCAAGG AGAGCCGCGA TCCCAGAGAC TCCCGGTCAG CTCGGGATGT CCATGACTAC AGGGACCGTG AAGCTCGGGA TGCTCATGCT CGAGATGTTC GAGATGCTAG GGATGCTCGG GATGCTAGGG ATGCTCGGGA TATTCGGGAT GTCCGTGATG TCCGGGATGT CCGGGATGTC CGGGATGTCC GGGATGTGCG AGATGTGCGT GATGTCCGAG ATGTTCGAGA TGCTCGCGAT GTCCGGGATG TCCGGGATGC TCGCGATGTC CGGGATGTCC GGGATGTTCG GGATGGTCAT CGAAAGGAGG ATGTATACCA GGAAGAAGCC CGCAGTTATG GCAGAAACCA CCTGAGAGAA GAGAGTTCTA GAGTTGAACT AAGGAATGAC TCTAGAAATG AGTCTCGAAG TGAAATTAGA AACGATCGGA TGGGCAGAAG TAGGGGGAGA GGTCCAGAAC TGCCTGAAAA GGGAAGTCGA GGAACAAGAG GTTCCCAAAT GGATAGCCAC AGTAGTGGCA GCAACTACCA TGACAGCTGG GAAACTCGCA GTAGCTATCC TGAAAGAGAC AGATACCCTG AGAGAGACAC TCGGGATCCG GCCAGGGATT CTTCCTTTGA GAGAAGGCAT GGAGAAAGAG ACCGTCGGGA TAACAGGGAA CGAGATCAAA GACCAAGCTC ACCTATTCGC CATCAGGGAA GGAGTGAGGA GCTTGAGCGA GATGAAAGAA GAGAGGAACG AAGAATAGAC AGAGTGGATG AGAGACGAGA CGATAGGGTC AGGGACAGAG ATAGGGATCG AGAGCGAGAA AGGGAGCGGG AGAGGGAACG AGAGCGGGAG CGAGAAAAGG AAAGAGAAAG AGAACTAGAA AGAGAGAGAG CTAGAGAGCG GGAAAGAGAG AGAGAGAAAG AGAGGGAGAG GGAGCGAGAA CGAGAGAGAG ATCAGCGAGA TCGTGACCAT GATCGAGAGC GAGAAAGAGA GAGGGAGCGA GAGAGGGAAA AAGAACGGGA GCGAGAGCGA GAAGAGAGAG AGAGGGAGAG AGAAAGGGAA AGGGAACGGG AGAGAGAGAG GGAACGGGAG CGGGAGAGAG AGCGAGAACG GGAGCGGGAG CGAGAGAGGG CCAGAGAAAG AGAAAAAGAA CGAGAACGGC AAAGAGAGTG GGAAGATAAA GACAAGGGAC GCGATGACCG GAGGGAGAAG CGCGAGGATA TTCATGTTCG AGAGGACAGG ATCCCCAGAG ACAGCCACGA GGAGAGGAAG TCCAAGAAAC GCTATAGAAA TGAAGGGAGT CCCAGCCCCC GGCAGTCCCC CAAGCGCCGG CGGGAGCATT CTCCTGACAG TGACACCTAC CACAGTGGGG ATGACAAAAA TGAAAAACAT AGACTCTTGA GCCAGGTTGT ACGGCCACAG GAATCTCGTT CTCTCAGCCC ATCCCACCTC ACAGAAGATA GGCAAGGTAG ATGGAAAGAA GAGGATCGTA AATCAGAAAG AAAGGAGAGT TCCAGGCGCT ACGAAGAGCA AGAACTCAAA GAGAAGCTTT CTTGTGGCGA CAGGCAAAGG GAGCAGGCCG AAAGCGTGGA CAGCTCGAGA GTGCGCGCCC AAGACCTCCT CAGCCATCGC CAGGCTGAAG ACCGCGACCG TGACGGGTCT GATCGAGCCC ATGATGAAAA GAAAAAAGCA AAAGCTCCGA AGAAACCTGT AAAGAAAAAG AAGGAGGAGG ATGTTGGAGT AGAGAGGGGC AACCTTGAGA CACATGAGGA TAGTCAGGTA TTTTCACCAA AAAAAGGACA GAAGAAGAAA AACATTGAAA AAAAGCGTAA AAGATCCAAG GGTGATTCTG ATGTTTCTGA TGAGGAGGCA GCCCCGCAGA ATAAGAAGAA GAGAGGGCCT CGCACACCCC CTCTGGCCAT CAAGGAGGAG CTGGCTGACA TCTCCACTGA CAAGGATGGC GTCCTGGAGG ATCCACTGAA GAAAGAAAAT ACAGCCTTTA GTGACTGGTC TGATGAGGAT GTGCCTGACC GCACAGAGGG GCTGGAAGCC GAGCACACCG CAGCCACTGC CACTCCTGGT AGCACCCCTT CCCCCCTGTC TTCTCTTCTC CCTCCTCCAC CCCCTGTGGC TGCTGCCAGT ACTGCAGCCA CTGCTCTTGC CTCCTCAGCT GTTTCTGCTA CCACCTCTGC CACCTCTTCA AGCTCTGCTG CCACCTCCAA TACCAATGGC AGTGAAGACT CACATAGAAA GTGCCACAGG GCACGAGGGG AGAAGGTAGA GGTGTCCCAT GTGACCCTAG AAGACACACC ACATCGCAAG CTGGTGGATC AAAAAAGAAG CAGCAGCCTC GGAAGCAATC GAAGTCACCG CAGCCACACC TCTGGTCGCC TACGCTCCCC ATCTAATGAT TCTGCTCATC GAAGTGGGGA TGACCAGGGT AGTCGGAAGA GAGTGCTCCA TAGTGGCTCC AGGGATAGAG AGAAAACAAA AAGCCTAGAA ATCACAGGAG AAAGAAAATC TAGGATTGAT CAGTTAAAGC GTGGAGAACC CAGTCGAAGT ACTTCTTCAG ATCGCCAGGA TTCCAGAAGC CATAGCTCAA GAAGAAGCTC TCCTGAGTCG GATCGGCAGG TTCACTCAAG GTCCGGGTCG TTTGACAGTA GAGACCGACT TCAAGAACGA GATAGGTATG AGCATGACAG AGAGCGGGAG AGGGACCGGA GAGATCCCAG ACAGCGGGAA TGGGATCGTG AAGCGGAGAA AGAGTGGCCT CGGACCAGAG ACCGAGACAG GCTTCGGGAA CGAGACAGAG ACCGGGACAG AAGGAGAGAC TTGGACAGAG AAAGAGAGAG ACTGATCTCT GACCCCATGG AAAGGGACAG AGAACGAGAG AGAACTTTTG AGACATCTCA GCTGGAATCT GGGAAACGAA GCGAAGTCAA ATTAGAGAGT GAACATGAGA GAGATCTGGA AGGCAGCTCC CGGGACTCCG TGGCCTTGGA CAAAGAAAGG ATGGACAGAG ACCTGGGCTC TGTGCAGGGA TTTGAAGATG TGAGTAAAGC TGAGAGAACA GAGAGTCTTG AAGCAGGAGA TGATGAATCC AAGTTAGATG ATGCACATTC ATTAGGATCT GGTGCTGGGG AAGGATATGA GCCCATCAGT GATGACGAGC TAGATGAAAT TCTGGCAGGG GATGCAGAAA AGAGAGAGGA CCAGCAGGAA GAAGAGAAGA TGCCAGATCC ATTAGATGTA ATAGATGTGG ACTGGTCTGG ACTAATGCCA AAGCACCCGA AAGAACCGAG AGAGCCTGGG GCTGCACTCT TAAAGTTCAC GCCTGGAGCT GTTCTGCTAC GAGTTGGGAT TTCTAAAAAG TTGGCTGGTT CTGAGCTCTT TACCAAAGTT AAAGAAACAT GTCAACAACT TGTAGAAAAA CCCAAAGATG CAGACAGCCT TTTCGAACAT GAACTAGGGG CTCTCAACAT GGCTGCTTTA CTTCGAAAAG AAGAGCGAGC AAGTCTTCTT AGTGACCTGG GACCATGTTG TAAAGCATTA TGTTTCAGAC GGGACTCTGC AATCCGGAAG CAGCTTGTGA AGAACGAAAA GGGAACTGTG AAGCAGGCGT ACACAAACAC TCCGATGGTA GACAATGAGC TGCTTCGGCT AAGTCTTCGG TTATTTAAGA AGAAGGCCAC CTGCCATGCC CCTGGACAGG AAAAGACTGA AGATGGCAAA TTAGGACCGT GCAGTATCCA GCAGGAGCTG TGTGTGTCCC TCGAGGACTA CAAAGACCAT GACGGTGATT ATAAAGATCA TGACATCGAC TACAAGGATG ACGATGACAA GTAGTCTAGA GGGCCCTATT CTATAGTGTC ACCTAAATGC TAGAGCTCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AAAGAACCAG CTGGGGCTCT AGGGGGTATC CCCACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG CATCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGGGGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTAATTCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCTGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCAATG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTC
