PIK47580.1，putative ELKS/Rab6-interacting/CAST family member 1 isoform X4
100μg liquid plasmid(Instantly):$91
| name | pAOX1-α-factor-ENA(apostichopus)-X4 | another name | apAOX1-α-factor-ENA(apostichopus)-X4 |
| Insert Size (bp) | 1698bp | Plasmid host | Yeast bacterium |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Apostichopus japonicus | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418,HIS4 | Fluorescent labeling | |
| Promoter | AOX1 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | 5'AOX：GACTGGTTCCAATTGACAAGC | |
| 3′ sequencing primer | 3'AOX：GCAAATGGCATTCTGACATCC | Copy number | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGATCTAACA TCCAAAGACG AAAGGTTGAA TGAAACCTTT TTGCCATCCG ACATCCACAG GTCCATTCTC ACACATAAGT GCCAAACGCA ACAGGAGGGG ATACACTAGC AGCAGACCGT TGCAAACGCA GGACCTCCAC TCCTCTTCTC CTCAACACCC ACTTTTGCCA TCGAAAAACC AGCCCAGTTA TTGGGCTTGA TTGGAGCTCG CTCATTCCAA TTCCTTCTAT TAGGCTACTA ACACCATGAC TTTATTAGCC TGTCTATCCT GGCCCCCCTG GCGAGGTTCA TGTTTGTTTA TTTCCGAATG CAACAAGCTC CGCATTACAC CCGAACATCA CTCCAGATGA GGGCTTTCTG AGTGTGGGGT CAAATAGTTT CATGTTCCCC AAATGGCCCA AAACTGACAG TTTAAACGCT GTCTTGGAAC CTAATATGAC AAAAGCGTGA TCTCATCCAA GATGAACTAA GTTTGGTTCG TTGAAATGCT AACGGCCAGT TGGTCAAAAA GAAACTTCCA AAAGTCGCCA TACCGTTTGT CTTGTTTGGT ATTGATTGAC GAATGCTCAA AAATAATCTC ATTAATGCTT AGCGCAGTCT CTCTATCGCT TCTGAACCCC GGTGCACCTG TGCCGAAACG CAAATGGGGA AACACCCGCT TTTTGGATGA TTATGCATTG TCTCCACATT GTATGCTTCC AAGATTCTGG TGGGAATACT GCTGATAGCC TAACGTTCAT GATCAAAATT TAACTGTTCT AACCCCTACT TGACAGCAAT ATATAAACAG AAGGAAGCTG CCCTGTCTTA AACCTTTTTT TTTATCATCA TTATTAGCTT ACTTTCATAA TTGCGACTGG TTCCAATTGA CAAGCTTTTG ATTTTAACGA CTTTTAACGA CAACTTGAGA AGATCAAAAA ACAACTAATT ATTCGAAGGA TCCAAACGAT GAGATTTCCT TCAATTTTTA CTGCAGTTTT ATTCGCAGCA TCCTCCGCAT TAGCTGCTCC AGTCAACACT ACAACAGAAG ATGAAACGGC ACAAATTCCG GCTGAAGCTG TCATCGGTTA CTCAGATTTA GAAGGGGATT TCGATGTTGC TGTTTTGCCA TTTTCCAACA GCACAAATAA CGGGTTATTG TTTATAAATA CTACTATTGC CAGCATTGCT GCTAAAGAAG AAGGGGTATC TCTCGAGAAA AGAGAGGCTG AAGCTTACGT AGAATTCATG TGTAAGGGGA CTTTTTACTT GGGACAAGAG GGATCCTCGC TGGAGAGAGT CAATGGTCTT CAATCAGCAG AAGTCGAAGA GCTGAGACAC CTTCGTCAAA AGATGGAGCA GGCCCAGGCA GACGTTGGAT CAAAGAATAA AGAGGTCCAG GCCCTTCACA CCAAGCTTAA TGCAATGGCC CAGCAATATC AAGATCAACA AGCTCACATT AGTCTGTTGA AGGAGTCACT AAAAGCCAAG GAGCAACACA TCAACATGTT GCAAGCTGAT ATGAATTCTT TACGTTCGCG AATAGAGGAG AAGGAGATGA TCCTGGCACA CCAGGACGAA CAGCTGAACA ACCTGTCATC CGATAAATCC AAAAGGTCAG GGGAGGTCAA CGAGCTCAAA GAAATCATCG ACCTCAAAGA GAGAAAGGTC GCTGTCTTAC AGAAGAAGAT TGACAATTTG CAAGAACAGC TTCAGGAGAA GAACCATCAA TTAGAAGACC TTCGTGGAAA GGTAAAGAGC CTGGAAGCAG ACAGCAAGAG CTCTGACAAT GCCATGACTA GCTTGGAGGA GGCCCTGATG GAGAAGGATA AATCATTAAT GCTCCTCAGA GAACAGAAGG AGAAGGAAGG GGTGAGCCTC AGGGAGGATT CAGAGAAGCA CCAGAGGCAG ACGCACGAGC TGACGGCCAG GATAGATGCC TTACAAAAAG ACGTTAACGA TAAAGAGTCT GGCCTGCTGG ATCTCAAGGA ACATGCTTCA CAGTTAGCAT CGGCCGGTTT GAAGAAGGAC TCTAAGATAA CCACGTTGGA AATTAATCTA GCTCAAACAA AGGAAGAACT TTGCCAAGCA GAAGAAGAGT TAAAGAAGTG GAAAGACGAG AAGGAGGCTG TGACAGGTCA GCAGGTCAAA GAATTAAACG AGAAGGCGGA GAAGAGCAAC CAGGAGGCCC AGAAGTTGCA GTCTGAGGTG GACAGAATAC TGGAAATCAT GAAAGATATG GAAGGAGAGA AGAATGACAA GGATAATCGA ATCAAGACAT TAGAAGGGCA ACTGAAGGAG GCTAACTTGA AATTTACCAG TATTAAAAAA GATCAACAAA ACCAGAAGAA GGCCAACCAA CAGCTCCTGG AGGAAGCGAA GAAACGAGAA GGGGAGCTGA CCACAGATAC ACATCAATTA CAGTCCATGG TAAAGGAGAA GAGTAACCGC TTGGAGGAGT TGGAAGAGGC TCTGAAGGAG AGTGTCAGTA TCACTGCTGA TCGAGAGATG ATCCTTGCTC AGCAGACCCA GACAGTCACC AAGTTACAGA AACAGCTGGA TGAGACTAAG AGAACTTTAC AAGCTGTACA GAAGAAACTT GCTGATAATG AGAACAAGCT GACAGCGACT CAAGCAATAT TAGAGGAGAA AGACAACAGA CTAAAGATCC TTGCAGCTGA AGGAAGGAAG CATTTAGAGG AAGCTCTGGA GACAAAGCAA GAGGCCCTGA CAGCTGCTAT CGGTGAGAAA GATGCTCACA TCGCTCTGTT AGAGCTGAAC AGGAAGGGAT CAAAGACATC GGATGAACTT AAATCACTCA AGAAAGAGAA GGATGCCTTG GTTCAACAGC TGAAAGATGA GACACAAAAG AGATTGAAGC TTCTCCACCA TAGCAGTGAC TCTGACAAGC TTTTACGAGG GAACTCTCCC ATTACTCCTG AACAGTAGCG GCCGCGAATT AATTCGCCTT AGACATGACT GTTCCTCAGT TCAAGTTGGG CACTTACGAG AAGACCGGTC TTGCTAGATT CTAATCAAGA GGATGTCAGA ATGCCATTTG CCTGAGAGAT GCAGGCTTCA TTTTTGATAC TTTTTTATTT GTAACCTATA TAGTATAGGA TTTTTTTTGT CATTTTGTTT CTTCTCGTAC GAGCTTGCTC CTGATCAGCC TATCTCGCAG CTGATGAATA TCTTGTGGTA GGGGTTTGGG AAAATCATTC GAGTTTGATG TTTTTCTTGG TATTTCCCAC TCCTCTTCAG AGTACAGAAG ATTAAGTGAG AAGTTCGTTT GTGCAAGCTT ATCGATAAGC TTTAATGCGG TAGTTTATCA CAGTTAAATT GCTAACGCAG TCAGGCACCG TGTATGAAAT CTAACAATGC GCTCATCGTC ATCCTCGGCA CCGTCACCCT GGATGCTGTA GGCATAGGCT TGGTTATGCC GGTACTGCCG GGCCTCTTGC GGGATATCGT CCATTCCGAC AGCATCGCCA GTCACTATGG CGTGCTGCTA GCGCTATATG CGTTGATGCA ATTTCTATGC GCACCCGTTC TCGGAGCACT GTCCGACCGC TTTGGCCGCC GCCCAGTCCT GCTCGCTTCG CTACTTGGAG CCACTATCGA CTACGCGATC ATGGCGACCA CACCCGTCCT GTGGATCTAT CGAATCTAAA TGTAAGTTAA AATCTCTAAA TAATTAAATA AGTCCCAGTT TCTCCATACG AACCTTAACA GCATTGCGGT GAGCATCTAG ACCTTCAACA GCAGCCAGAT CCATCACTGC TTGGCCAATA TGTTTCAGTC CCTCAGGAGT TACGTCTTGT GAAGTGATGA ACTTCTGGAA GGTTGCAGTG TTAACTCCGC TGTATTGACG GGCATATCCG TACGTTGGCA AAGTGTGGTT GGTACCGGAG GAGTAATCTC CACAACTCTC TGGAGAGTAG GCACCAACAA ACACAGATCC AGCGTGTTGT ACTTGATCAA CATAAGAAGA AGCATTCTCG ATTTGCAGGA TCAAGTGTTC AGGAGCGTAC TGATTGGACA TTTCCAAAGC CTGCTCGTAG GTTGCAACCG ATAGGGTTGT AGAGTGTGCA ATACACTTGC GTACAATTTC AACCCTTGGC AACTGCACAG CTTGGTTGTG AACAGCATCT TCAATTCTGG CAAGCTCCTT GTCTGTCATA TCGACAGCCA ACAGAATCAC CTGGGAATCA ATACCATGTT CAGCTTGAGA CAGAAGGTCT GAGGCAACGA AATCTGGATC AGCGTATTTA TCAGCAATAA CTAGAACTTC AGAAGGCCCA GCAGGCATGT CAATACTACA CAGGGCTGAT GTGTCATTTT GAACCATCAT CTTGGCAGCA GTAACGAACT GGTTTCCTGG ACCAAATATT TTGTCACACT TAGGAACAGT TTCTGTTCCG TAAGCCATAG CAGCTACTGC CTGGGCGCCT CCTGCTAGCA CGATACACTT AGCACCAACC TTGTGGGCAA CGTAGATGAC TTCTGGGGTA AGGGTACCAT CCTTCTTAGG TGGAGATGCA AAAACAATTT CTTTGCAACC AGCAACTTTG GCAGGAACAC CCAGCATCAG GGAAGTGGAA GGCAGAATTG CGGTTCCACC AGGAATATAG AGGCCAACTT TCTCAATAGG TCTTGCAAAA CGAGAGCAGA CTACACCAGG GCAAGTCTCA ACTTGCAACG TCTCCGTTAG TTGAGCTTCA TGGAATTTCC TGACGTTATC TATAGAGAGA TCAATGGCTC TCTTAACGTT ATCTGGCAAT TGCATAAGTT CCTCTGGGAA AGGAGCTTCT AACACAGGTG TCTTCAAAGC GACTCCATCA AACTTGGCAG TTAGTTCTAA AAGGGCTTTG TCACCATTTT GACGAACATT GTCGACAATT GGTTTGACTA ATTCCATAAT CTGTTCCGTT TTCTGGATAG GACGACGAAG GGCATCTTCA ATTTCTTGTG AGGAGGCCTT AGAAACGTCA ATTTTGCACA ATTCAATACG ACCTTCAGAA GGGACTTCTT TAGGTTTGGA TTCTTCTTTA GGTTGTTCCT TGGTGTATCC TGGCTTGGCA TCTCCTTTCC TTCTAGTGAC CTTTAGGGAC TTCATATCCA GGTTTCTCTC CACCTCGTCC AACGTCACAC CGTACTTGGC ACATCTAACT AATGCAAAAT AAAATAAGTC AGCACATTCC CAGGCTATAT CTTCCTTGGA TTTAGCTTCT GCAAGTTCAT CAGCTTCCTC CCTAATTTTA GCGTTCAACA AAACTTCGTC GTCAAATAAC CGTTTGGTAT AAGAACCTTC TGGAGCATTG CTCTTACGAT CCCACAAGGT GGCTTCCATG GCTCTAAGAC CCTTTGATTG GCCAAAACAG GAAGTGCGTT CCAAGTGACA GAAACCAACA CCTGTTTGTT CAACCACAAA TTTCAAGCAG TCTCCATCAC AATCCAATTC GATACCCAGC AACTTTTGAG TTGCTCCAGA TGTAGCACCT TTATACCACA AACCGTGACG ACGAGATTGG TAGACTCCAG TTTGTGTCCT TATAGCCTCC GGAATAGACT TTTTGGACGA GTACACCAGG CCCAACGAGT AATTAGAAGA GTCAGCCACC AAAGTAGTGA ATAGACCATC GGGGCGGTCA GTAGTCAAAG ACGCCAACAA AATTTCACTG ACAGGGAACT TTTTGACATC TTCAGAAAGT TCGTATTCAG TAGTCAATTG CCGAGCATCA ATAATGGGGA TTATACCAGA AGCAACAGTG GAAGTCACAT CTACCAACTT TGCGGTCTCA GAAAAAGCAT AAACAGTTCT ACTACCGCCA TTAGTGAAAC TTTTCAAATC GCCCAGTGGA GAAGAAAAAG GCACAGCGAT ACTAGCATTA GCGGGCAAGG ATGCAACTTT ATCAACCAGG GTCCTATAGA TAACCCTAGC GCCTGGGATC ATCCTTTGGA CAACTCTTTC TGCCAAATCT AGGTCCAAAA TCACTTCATT GATACCATTA TTGTACAACT TGAGCAAGTT GTCGATCAGC TCCTCAAATT GGTCCTCTGT AACGGATGAC TCAACTTGCA CATTAACTTG AAGCTCAGTC GATTGAGTGA ACTTGATCAG GTTGTGCAGC TGGTCAGCAG CATAGGGAAA CACGGCTTTT CCTACCAAAC TCAAGGAATT ATCAAACTCT GCAACACTTG CGTATGCAGG TAGCAAGGGA AATGTCATAC TTGAAGTCGG ACAGTGAGTG TAGTCTTGAG AAATTCTGAA GCCGTATTTT TATTATCAGT GAGTCAGTCA TCAGGAGATC CTCTACGCCG GACGCATCGT GGCCGACCTG CAGGGGGGGG GGGGGCGCTG AGGTCTGCCT CGTGAAGAAG GTGTTGCTGA CTCATACCAG GCCTGAATCG CCCCATCATC CAGCCAGAAA GTGAGGGAGC CACGGTTGAT GAGAGCTTTG TTGTAGGTGG ACCAGTTGGT GATTTTGAAC TTTTGCTTTG CCACGGAACG GTCTGCGTTG TCGGGAAGAT GCGTGATCTG ATCCTTCAAC TCAGCAAAAG TTCGATTTAT TCAACAAAGC CGCCGTCCCG TCAAGTCAGC GTAATGCTCT GCCAGTGTTA CAACCAATTA ACCAATTCTG ATTAGAAAAA CTCATCGAGC ATCAAATGAA ACTGCAATTT ATTCATATCA GGATTATCAA TACCATATTT TTGAAAAAGC CGTTTCTGTA ATGAAGGAGA AAACTCACCG AGGCAGTTCC ATAGGATGGC AAGATCCTGG TATCGGTCTG CGATTCCGAC TCGTCCAACA TCAATACAAC CTATTAATTT CCCCTCGTCA AAAATAAGGT TATCAAGTGA GAAATCACCA TGAGTGACGA CTGAATCCGG TGAGAATGGC AAAAGCTTAT GCATTTCTTT CCAGACTTGT TCAACAGGCC AGCCATTACG CTCGTCATCA AAATCACTCG CATCAACCAA ACCGTTATTC ATTCGTGATT GCGCCTGAGC GAGACGAAAT ACGCGATCGC TGTTAAAAGG ACAATTACAA ACAGGAATCG AATGCAACCG GCGCAGGAAC ACTGCCAGCG CATCAACAAT ATTTTCACCT GAATCAGGAT ATTCTTCTAA TACCTGGAAT GCTGTTTTCC CGGGGATCGC AGTGGTGAGT AACCATGCAT CATCAGGAGT ACGGATAAAA TGCTTGATGG TCGGAAGAGG CATAAATTCC GTCAGCCAGT TTAGTCTGAC CATCTCATCT GTAACATCAT TGGCAACGCT ACCTTTGCCA TGTTTCAGAA ACAACTCTGG CGCATCGGGC TTCCCATACA ATCGATAGAT TGTCGCACCT GATTGCCCGA CATTATCGCG AGCCCATTTA TACCCATATA AATCAGCATC CATGTTGGAA TTTAATCGCG GCCTCGAGCA AGACGTTTCC CGTTGAATAT GGCTCATAAC ACCCCTTGTA TTACTGTTTA TGTAAGCAGA CAGTTTTATT GTTCATGATG ATATATTTTT ATCTTGTGCA ATGTAACATC AGAGATTTTG AGACACAACG TGGCTTTCCC CCCCCCCCCT GCAGGTCGGC ATCACCGGCG CCACAGGTGC GGTTGCTGGC GCCTATATCG CCGACATCAC CGATGGGGAA GATCGGGCTC GCCACTTCGG GCTCATGAGC GCTTGTTTCG GCGTGGGTAT GGTGGCAGGC CCCGTGGCCG GGGGACTGTT GGGCGCCATC TCCTTGCATG CACCATTCCT TGCGGCGGCG GTGCTCAACG GCCTCAACCT ACTACTGGGC TGCTTCCTAA TGCAGGAGTC GCATAAGGGA GAGCGTCGAG TATCTATGAT TGGAAGTATG GGAATGGTGA TACCCGCATT CTTCAGTGTC TTGAGGTCTC CTATCAGATT ATGCCCAACT AAAGCAACCG GAGGAGGAGA TTTCATGGTA AATTTCTCTG ACTTTTGGTC ATCAGTAGAC TCGAACTGTG AGACTATCTC GGTTATGACA GCAGAAATGT CCTTCTTGGA GACAGTAAAT GAAGTCCCAC CAATAAAGAA ATCCTTGTTA TCAGGAACAA ACTTCTTGTT TCGAACTTTT TCGGTGCCTT GAACTATAAA ATGTAGAGTG GATATGTCGG GTAGGAATGG AGCGGGCAAA TGCTTACCTT CTGGACCTTC AAGAGGTATG TAGGGTTTGT AGATACTGAT GCCAACTTCA GTGACAACGT TGCTATTTCG TTCAAACCAT TCCGAATCCA GAGAAATCAA AGTTGTTTGT CTACTATTGA TCCAAGCCAG TGCGGTCTTG AAACTGACAA TAGTGTGCTC GTGTTTTGAG GTCATCTTTG TATGAATAAA TCTAGTCTTT GATCTAAATA ATCTTGACGA GCCAAGGCGA TAAATACCCA AATCTAAAAC TCTTTTAAAA CGTTAAAAGG ACAAGTATGT CTGCCTGTAT TAAACCCCAA ATCAGCTCGT AGTCTGATCC TCATCAACTT GAGGGGCACT ATCTTGTTTT AGAGAAATTT GCGGAGATGC GATATCGAGA AAAAGGTACG CTGATTTTAA ACGTGAAATT TATCTCAAGA TCTCTGCCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCGCAGC CATGACCCAG TCACGTAGCG ATAGCGGAGT GTATACTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCAATGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTGCAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAACACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGTC TTCAAGAATT AATTCTCATG TTTGACAGCT TATCATCGAT AAGCTGACTC ATGTTGGTAT TGTGAAATAG ACGCAGATCG GGAACACTGA AAAATAACAG TTATTATTCG
