PiggyBac-CMV-SCN2A(human)-Puro
$142.00
In stock
SKU
PL32870
100μg liquid plasmid(Instantly):$163
| name | PiggyBac-CMV-SCN2A(human)-Puro | another name | aPiggyBac-CMV-SCN2A(human)-Puro |
| Insert Size (bp) | 6018bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression,Transpose | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | BL21(DE3) | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAGCGC GCCTCGTTCA TTCACGTTTT TGAACCCGTG GAGGACGGGC AGACTCGCGG TGCAAATGTG TTTTACAGCG TGATGGAGCA GATGAAGATG CTCGACACGC TGCAGAACAC GCAGCTAGAT TAACCCTAGA AAGATAATCA TATTGTGACG TACGTTAAAG ATAATCATGC GTAAAATTGA CGCATGTGTT TTATCGGTCT GTATATCGAG GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA TTTACACTTA CATACTAATA ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT TAAAAAAAAA CAAAAACTCA AAATTTCTTC TATAAAGTAA CAAAACTTTT ATGAGGGACA GCCCCCCCCC AAAGCCCCCA GGGATGTAAT TACGTCCCTC CCCCGCTAGG GGGCAGCAGC GAGCCGCCCG GGGCTCCGCT CCGGTCCGGC GCTCCCCCCG CATCCCCGAG CCGGCAGCGT GCGGGGACAG CCCGGGCACG GGGAAGGTGG CACGGGATCG CTTTCCTCTG AACGCTTCTC GCTGCTCTTT GAGCCTGCAG ACACCTGGGG GGATACGGGG AAAAGGCCTC CAAGGCCTAC TAGTATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCGCCT GGAGACGCCA TCCACGCTGT TTTGACCTCC ATAGAAGATT CTAGAGCCAC CATGGCACAG TCAGTGCTGG TACCGCCAGG ACCTGACAGC TTCCGCTTCT TTACCAGGGA ATCCCTTGCT GCTATTGAAC AACGCATTGC AGAAGAGAAA GCTAAGAGAC CCAAACAGGA ACGCAAGGAT GAGGATGATG AAAATGGCCC AAAGCCAAAC AGTGACTTGG AAGCAGGAAA ATCTCTTCCA TTTATTTATG GAGACATTCC TCCAGAGATG GTGTCAGTGC CCCTGGAGGA TCTGGACCCC TACTATATCA ATAAGAAAAC GTTTATAGTA TTGAATAAAG GGAAAGCAAT CTCTCGATTC AGTGCCACCC CTGCCCTTTA CATTTTAACT CCCTTCAACC CTATTAGAAA ATTAGCTATT AAGATTTTGG TACATTCTTT ATTCAATATG CTCATTATGT GCACGATTCT TACCAACTGT GTATTTATGA CCATGAGTAA CCCTCCAGAC TGGACAAAGA ATGTGGAGTA TACCTTTACA GGAATTTATA CTTTTGAATC ACTTATTAAA ATACTTGCAA GGGGCTTTTG TTTAGAAGAT TTCACATTTT TACGGGATCC ATGGAATTGG TTGGATTTCA CAGTCATTAC TTTTGCATAT GTGACAGAGT TTGTGGACCT GGGCAATGTC TCAGCGTTGA GAACATTCAG AGTTCTCCGA GCATTGAAAA CAATTTCAGT CATTCCAGGC CTGAAGACCA TTGTGGGGGC CCTGATCCAG TCAGTGAAGA AGCTTTCTGA TGTCATGATC TTGACTGTGT TCTGTCTAAG CGTGTTTGCG CTAATAGGAT TGCAGTTGTT CATGGGCAAC CTACGAAATA AATGTTTGCA ATGGCCTCCA GATAATTCTT CCTTTGAAAT AAATATCACT TCCTTCTTTA ACAATTCATT GGATGGGAAT GGTACTACTT TCAATAGGAC AGTGAGCATA TTTAACTGGG ATGAATATAT TGAGGATAAA AGTCACTTTT ATTTTTTAGA GGGGCAAAAT GATGCTCTGC TTTGTGGCAA CAGCTCAGAT GCAGGCCAGT GTCCTGAAGG ATACATCTGT GTGAAGGCTG GTAGAAACCC CAACTATGGC TACACGAGCT TTGACACCTT TAGTTGGGCC TTTTTGTCCT TATTTCGTCT CATGACTCAA GACTTCTGGG AAAACCTTTA TCAACTGACA CTACGTGCTG CTGGGAAAAC GTACATGATA TTTTTTGTGC TGGTCATTTT CTTGGGCTCA TTCTATCTAA TAAATTTGAT CTTGGCTGTG GTGGCCATGG CCTATGAGGA ACAGAATCAG GCCACATTGG AAGAGGCTGA ACAGAAGGAA GCTGAATTTC AGCAGATGCT CGAACAGTTG AAAAAGCAAC AAGAAGAAGC TCAGGCGGCA GCTGCAGCCG CATCTGCTGA ATCAAGAGAC TTCAGTGGTG CTGGTGGGAT AGGAGTTTTT TCAGAGAGTT CTTCAGTAGC ATCTAAGTTG AGCTCCAAAA GTGAAAAAGA GCTGAAAAAC AGAAGAAAGA AAAAGAAACA GAAAGAACAG TCTGGAGAAG AAGAGAAAAA TGACAGAGTC CGAAAATCGG AATCTGAAGA CAGCATAAGA AGAAAAGGTT TCCGTTTTTC CTTGGAAGGA AGTAGGCTGA CATATGAAAA GAGATTTTCT TCTCCACACC AGTCCTTACT GAGCATCCGT GGCTCCCTTT TCTCTCCAAG ACGCAACAGT AGGGCGAGCC TTTTCAGCTT CAGAGGTCGA GCAAAGGACA TTGGCTCTGA GAATGACTTT GCTGATGATG AGCACAGCAC CTTTGAGGAC AATGACAGCC GAAGAGACTC TCTGTTCGTG CCGCACAGAC ATGGAGAACG GCGCCACAGC AATGTCAGCC AGGCCAGCCG TGCCTCCAGG GTGCTCCCCA TCCTGCCCAT GAATGGGAAG ATGCATAGCG CTGTGGACTG CAATGGTGTG GTCTCCCTGG TCGGGGGCCC TTCTACCCTC ACATCTGCTG GGCAGCTCCT ACCAGAGGGC ACAACTACTG AAACAGAAAT AAGAAAGAGA CGGTCCAGTT CTTATCATGT TTCCATGGAT TTATTGGAAG ATCCTACATC AAGGCAAAGA GCAATGAGTA TAGCCAGTAT TTTGACCAAC ACCATGGAAG AACTTGAAGA ATCCAGACAG AAATGCCCAC CATGCTGGTA TAAATTTGCT AATATGTGTT TGATTTGGGA CTGTTGTAAA CCATGGTTAA AGGTGAAACA CCTTGTCAAC CTGGTTGTAA TGGACCCATT TGTTGACCTG GCCATCACCA TCTGCATTGT CTTAAATACA CTCTTCATGG CTATGGAGCA CTATCCCATG ACGGAGCAGT TCAGCAGTGT ACTGTCTGTT GGAAACCTGG TCTTCACAGG GATCTTCACA GCAGAAATGT TTCTCAAGAT AATTGCCATG GATCCATATT ATTACTTTCA AGAAGGCTGG AATATTTTTG ATGGTTTTAT TGTGAGCCTT AGTTTAATGG AACTTGGTTT GGCAAATGTG GAAGGATTGT CAGTTCTCCG ATCATTCCGG CTGCTCCGAG TTTTCAAGTT GGCAAAATCT TGGCCAACTC TAAATATGCT AATTAAGATC ATTGGCAATT CTGTGGGGGC TCTAGGAAAC CTCACCTTGG TATTGGCCAT CATCGTCTTC ATTTTTGCTG TGGTCGGCAT GCAGCTCTTT GGTAAGAGCT ACAAAGAATG TGTCTGCAAG ATTTCCAATG ATTGTGAACT CCCACGCTGG CACATGCATG ACTTTTTCCA CTCCTTCCTG ATCGTGTTCC GCGTGCTGTG TGGAGAGTGG ATAGAGACCA TGTGGGACTG TATGGAGGTC GCTGGCCAAA CCATGTGCCT TACTGTCTTC ATGATGGTCA TGGTGATTGG AAATCTAGTG GTTCTGAACC TCTTCTTGGC CTTGCTTTTG AGTTCCTTCA GTTCTGACAA TCTTGCTGCC ACTGATGATG ATAACGAAAT GAATAATCTC CAGATTGCTG TGGGAAGGAT GCAGAAAGGA ATCGATTTTG TTAAAAGAAA AATACGTGAA TTTATTCAGA AAGCCTTTGT TAGGAAGCAG AAAGCTTTAG ATGAAATTAA ACCGCTTGAA GATCTAAATA ATAAAAAAGA CAGCTGTATT TCCAACCATA CCACCATAGA AATAGGCAAA GACCTCAATT ATCTCAAAGA CGGAAATGGA ACTACTAGTG GCATAGGCAG CAGTGTAGAA AAATATGTCG TGGATGAAAG TGATTACATG TCATTTATAA ACAACCCTAG CCTCACTGTG ACAGTACCAA TTGCTGTTGG AGAATCTGAC TTTGAAAATT TAAATACTGA AGAATTCAGC AGCGAGTCAG ATATGGAGGA AAGCAAAGAG AAGCTAAATG CAACTAGTTC ATCTGAAGGC AGCACGGTTG ATATTGGAGC TCCCGCCGAG GGAGAACAGC CTGAGGTTGA ACCTGAGGAA TCCCTTGAAC CTGAAGCCTG TTTTACAGAA GACTGTGTAC GGAAGTTCAA GTGTTGTCAG ATAAGCATAG AAGAAGGCAA AGGGAAACTC TGGTGGAATT TGAGGAAAAC ATGCTATAAG ATAGTGGAGC ACAATTGGTT CGAAACCTTC ATTGTCTTCA TGATTCTGCT GAGCAGTGGG GCTCTGGCCT TTGAAGATAT ATACATTGAG CAGCGAAAAA CCATTAAGAC CATGTTAGAA TATGCTGACA AGGTTTTCAC TTACATATTC ATTCTGGAAA TGCTGCTAAA GTGGGTTGCA TATGGTTTTC AAGTGTATTT TACCAATGCC TGGTGCTGGC TAGACTTCCT GATTGTTGAT GTCTCACTGG TTAGCTTAAC TGCAAATGCC TTGGGTTACT CAGAACTTGG TGCCATCAAA TCCCTCAGAA CACTAAGAGC TCTGAGGCCA CTGAGAGCTT TGTCCCGGTT TGAAGGAATG AGGGTTGTTG TAAATGCTCT TTTAGGAGCC ATTCCATCTA TCATGAATGT ACTTCTGGTT TGTCTGATCT TTTGGCTAAT ATTCAGTATC ATGGGAGTGA ATCTCTTTGC TGGCAAGTTT TACCATTGTA TTAATTACAC CACTGGAGAG ATGTTTGATG TAAGCGTGGT CAACAACTAC AGTGAGTGCA AAGCTCTCAT TGAGAGCAAT CAAACTGCCA GGTGGAAAAA TGTGAAAGTA AACTTTGATA ACGTAGGACT TGGATATCTG TCTCTACTTC AAGTAGCCAC GTTTAAGGGA TGGATGGATA TTATGTATGC AGCTGTTGAT TCACGAAATG TAGAATTACA ACCCAAGTAT GAAGACAACC TGTACATGTA TCTTTATTTT GTCATCTTTA TTATTTTTGG TTCATTCTTT ACCTTGAATC TTTTCATTGG TGTCATCATA GATAACTTCA ACCAACAGAA AAAGAAGTTT GGAGGTCAAG ACATTTTTAT GACAGAAGAA CAGAAGAAAT ACTACAATGC AATGAAAAAA CTGGGTTCAA AGAAACCACA AAAACCCATA CCTCGACCTG CTAACAAATT CCAAGGAATG GTCTTTGATT TTGTAACCAA ACAAGTCTTT GATATCAGCA TCATGATCCT CATCTGCCTT AACATGGTCA CCATGATGGT GGAAACCGAT GACCAGAGTC AAGAAATGAC AAACATTCTG TACTGGATTA ATCTGGTGTT TATTGTTCTG TTCACTGGAG AATGTGTGCT GAAACTGATC TCTCTTCGTT ACTACTATTT CACTATTGGA TGGAATATTT TTGATTTTGT GGTGGTCATT CTCTCCATTG TAGGAATGTT TCTGGCTGAA CTGATAGAAA AGTATTTTGT GTCCCCTACC CTGTTCCGAG TGATCCGTCT TGCCAGGATT GGCCGAATCC TACGTCTGAT CAAAGGAGCA AAGGGGATCC GCACGCTGCT CTTTGCTTTG ATGATGTCCC TTCCTGCGTT GTTTAACATC GGCCTCCTTC TTTTCCTGGT CATGTTCATC TACGCCATCT TTGGGATGTC CAATTTTGCC TATGTTAAGA GGGAAGTTGG GATCGATGAC ATGTTCAACT TTGAGACCTT TGGCAACAGC ATGATCTGCC TGTTCCAAAT TACAACCTCT GCTGGCTGGG ATGGATTGCT AGCACCTATT CTTAATAGTG GACCTCCAGA CTGTGACCCT GACAAAGATC ACCCTGGAAG CTCAGTTAAA GGAGACTGTG GGAACCCATC TGTTGGGATT TTCTTTTTTG TCAGTTACAT CATCATATCC TTCCTGGTTG TGGTGAACAT GTACATCGCG GTCATCCTGG AGAACTTCAG TGTTGCTACT GAAGAAAGTG CAGAGCCTCT GAGTGAGGAT GACTTTGAGA TGTTCTATGA GGTTTGGGAG AAGTTTGATC CCGATGCGAC CCAGTTTATA GAGTTTGCCA AACTTTCTGA TTTTGCAGAT GCCCTGGATC CTCCTCTTCT CATAGCAAAA CCCAACAAAG TCCAGCTCAT TGCCATGGAT CTGCCCATGG TGAGTGGTGA CCGGATCCAC TGTCTTGACA TCTTATTTGC TTTTACAAAG CGTGTTTTGG GTGAGAGTGG AGAGATGGAT GCCCTTCGAA TACAGATGGA AGAGCGATTC ATGGCATCAA ACCCCTCCAA AGTCTCTTAT GAGCCCATTA CGACCACGTT GAAACGCAAA CAAGAGGAGG TGTCTGCTAT TATTATCCAG AGGGCTTACA GACGCTACCT CTTGAAGCAA AAAGTTAAAA AGGTATCAAG TATATACAAG AAAGACAAAG GCAAAGAATG TGATGGAACA CCCATCAAAG AAGATACTCT CATTGATAAA CTGAATGAGA ATTCAACTCC AGAGAAAACC GATATGACGC CTTCCACCAC GTCTCCACCC TCGTATGATA GTGTGACCAA ACCAGAAAAA GAAAAATTTG AAAAAGACAA ATCAGAAAAG GAAGACAAAG GGAAAGATAT CAGGGAAAGT AAAAAGTAAG CGGCCGCGAA GGATCTGCGA TCGCTCCGGT GCCCGTCAGT GGGCAGAGCG CACATCGCCC ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC GGCAATTGAA CGGGTGCCTA GAGAAGGTGG CGCGGGGTAA ACTGGGAAAG TGATGTCGTG TACTGGCTCC GCCTTTTTCC CGAGGGTGGG GGAGAACCGT ATATAAGTGC AGTAGTCGCC GTGAACGTTC TTTTTCGCAA CGGGTTTGCC GCCAGAACAC AGCTGAAGCT TCGAGGGGCT CGCATCTCTC CTTCACGCGC CCGCCGCCCT ACCTGAGGCC GCCATCCACG CCGGTTGAGT CGCGTTCTGC CGCCTCCCGC CTGTGGTGCC TCCTGAACTG CGTCCGCCGT CTAGGTAAGT TTAAAGCTCA GGTCGAGACC GGGCCTTTGT CCGGCGCTCC CTTGGAGCCT ACCTAGACTC AGCCGGCTCT CCACGCTTTG CCTGACCCTG CTTGCTCAAC TCTACGTCTT TGTTTCGTTT TCTGTTCTGC GCCGTTACAG ATCCAAGCTG TGACCGGCGC CTACGCTAGA CGCCACCATG ACCGAGTACA AGCCCACGGT GCGCCTCGCC ACCCGCGACG ACGTCCCCAG GGCCGTACGC ACCCTCGCCG CCGCGTTCGC CGACTACCCC GCCACGCGCC ACACCGTCGA TCCGGACCGC CACATCGAGC GGGTCACCGA GCTGCAAGAA CTCTTCCTCA CGCGCGTCGG GCTCGACATC GGCAAGGTGT GGGTCGCGGA CGACGGCGCC GCGGTGGCGG TCTGGACCAC GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT CGCCGAGATC GGCCCGCGCA TGGCCGAGTT GAGCGGTTCC CGGCTGGCCG CGCAGCAACA GATGGAAGGC CTCCTGGCGC CGCACCGGCC CAAGGAGCCC GCGTGGTTCC TGGCCACCGT CGGCGTCTCG CCCGACCACC AGGGCAAGGG TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT GGAGGCGGCC GAGCGCGCCG GGGTGCCCGC CTTCCTGGAG ACCTCCGCGC CCCGCAACCT CCCCTTCTAC GAGCGGCTCG GCTTCACCGT CACCGCCGAC GTCGAGGTGC CCGAAGGACC GCGCACCTGG TGCATGACCC GCAAGCCCGG TGCCTGAAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC AGTTAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGAATT GACTCAAATG ATGTCAATTA GTCTATCAGA AGCTATCTGG TCTCCCTTCC GGGGGACAAG ACATCCCTGT TTAATATTTA AACAGCAGTG TTCCCAAACT GGGTTCTTAT ATCCCTTGCT CTGGTCAACC AGGTTGCAGG GTTTCCTGTC CTCACAGGAA CGAAGTCCCT AAAGAAACAG TGGCAGCCAG GTTTAGCCCC GGAATTGACT GGATTCCTTT TTTAGGGCCC ATTGGTATGG CTTTTTCCCC GTATCCCCCC AGGTGTCTGC AGGCTCAAAG AGCAGCGAGA AGCGTTCAGA GGAAAGCGAT CCCGTGCCAC CTTCCCCGTG CCCGGGCTGT CCCCGCACGC TGCCGGCTCG GGGATGCGGG GGGAGCGCCG GACCGGAGCG GAGCCCCGGG CGGCTCGCTG CTGCCCCCTA GCGGGGGAGG GACGTAATTA CATCCCTGGG GGCTTTGGGG GGGGGCTGTC CCTGATATCT ATAACAAGAA AATATATATA TAATAAGTTA TCACGTAAGT AGAACATGAA ATAACAATAT AATTATCGTA TGAGTTAAAT CTTAAAAGTC ACGTAAAAGA TAATCATGCG TCATTTTGAC TCACGCGGTC GTTATAGTTC AAAATCAGTG ACACTTACCG CATTGACAAG CACGCCTCAC GGGAGCTCCA AGCGGCGACT GAGATGTCCT AAATGCACAG CGACGGATTC GCGCTATTTA GAAAGAGAGA GCAATATTTC AAGAATGCAT GCGTCAATTT TACGCAGACT ATCTTTCTAG GGTTAATCTA GCTGCATCAG GATCATATCG TCGGGTCTTT TTTCCGGCTC AGTCATCGCC CAAGCTGGCG CTATCTGGGC ATCGGGGAGG AAGAAGCCCG TGCCTTTTCC CGCGAGGTTG AAGCGGCATG GAAAGAGTTT GCCGAGGATG ACTGCTGCTG CATTGACGTT GAGCGAAAAC GCACGTTTAC CATGATGATT CGGGAAGGTG TGGCCATGCA CGCCTTTAAC GGTGAACTGT TCGTTCAGGC CACCTGGGAT ACCAGTTCGT CGCGGCTTTT CCGGACACAG TTCCGGATGG TCAGCCCGAA GCGCATCAGC AACCCGAACA ATACCGGCGA CAGCCGGAAC TGCCGTGCCG GTGTGCAGAT TAATGACAGC GGTGCGGCGC TGGGATATTA CGTCAGCGAG GACGGGTATC CTGGCTGGAT GCCGCAGAAA TGGACATGGA TACCCCGTGA GTTACCCGGC GGGCGCGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCAT
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