NM_000238.4;ERG-1; ERG1; H-ERG; HERG; HERG1; Kv11.1;LQT2; SQT1;
100μg liquid plasmid(Instantly):$120
| name | pCMV-KCNH2(human)-3×FLAG-mCMV-EGFP | another name | apCMV-KCNH2(human)-3×FLAG-mCMV-EGFP |
| Insert Size (bp) | 3477bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGACTCAG ATCTCGAGCT CCGAATTCGC CACCATGCCG GTGCGGAGGG GCCACGTCGC GCCGCAGAAC ACCTTCCTGG ACACCATCAT CCGCAAGTTT GAGGGCCAGA GCCGTAAGTT CATCATCGCC AACGCTCGGG TGGAGAACTG CGCCGTCATC TACTGCAACG ACGGCTTCTG CGAGCTGTGC GGCTACTCGC GGGCCGAGGT GATGCAGCGA CCCTGCACCT GCGACTTCCT GCACGGGCCG CGCACGCAGC GCCGCGCTGC CGCGCAGATC GCGCAGGCAC TGCTGGGCGC CGAGGAGCGC AAAGTGGAAA TCGCCTTCTA CCGGAAAGAT GGGAGCTGCT TCCTATGTCT GGTGGATGTG GTGCCCGTGA AGAACGAGGA TGGGGCTGTC ATCATGTTCA TCCTCAATTT CGAGGTGGTG ATGGAGAAGG ACATGGTGGG GTCCCCGGCT CATGACACCA ACCACCGGGG CCCCCCCACC AGCTGGCTGG CCCCAGGCCG CGCCAAGACC TTCCGCCTGA AGCTGCCCGC GCTGCTGGCG CTGACGGCCC GGGAGTCGTC GGTGCGGTCG GGCGGCGCGG GCGGCGCGGG CGCCCCGGGG GCCGTGGTGG TGGACGTGGA CCTGACGCCC GCGGCACCCA GCAGCGAGTC GCTGGCCCTG GACGAAGTGA CAGCCATGGA CAACCACGTG GCAGGGCTCG GGCCCGCGGA GGAGCGGCGT GCGCTGGTGG GTCCCGGCTC TCCGCCCCGC AGCGCGCCCG GCCAGCTCCC ATCGCCCCGG GCGCACAGCC TCAACCCCGA CGCCTCGGGC TCCAGCTGCA GCCTGGCCCG GACGCGCTCC CGAGAAAGCT GCGCCAGCGT GCGCCGCGCC TCGTCGGCCG ACGACATCGA GGCCATGCGC GCCGGGGTGC TGCCCCCGCC ACCGCGCCAC GCCAGCACCG GGGCCATGCA CCCACTGCGC AGCGGCTTGC TCAACTCCAC CTCGGACTCC GACCTCGTGC GCTACCGCAC CATTAGCAAG ATTCCCCAAA TCACCCTCAA CTTTGTGGAC CTCAAGGGCG ACCCCTTCTT GGCTTCGCCC ACCAGTGACC GTGAGATCAT AGCACCTAAG ATAAAGGAGC GAACCCACAA TGTCACTGAG AAGGTCACCC AGGTCCTGTC CCTGGGCGCC GACGTGCTGC CTGAGTACAA GCTGCAGGCA CCGCGCATCC ACCGCTGGAC CATCCTGCAT TACAGCCCCT TCAAGGCCGT GTGGGACTGG CTCATCCTGC TGCTGGTCAT CTACACGGCT GTCTTCACAC CCTACTCGGC TGCCTTCCTG CTGAAGGAGA CGGAAGAAGG CCCGCCTGCT ACCGAGTGTG GCTACGCCTG CCAGCCGCTG GCTGTGGTGG ACCTCATCGT GGACATCATG TTCATTGTGG ACATCCTCAT CAACTTCCGC ACCACCTACG TCAATGCCAA CGAGGAGGTG GTCAGCCACC CCGGCCGCAT CGCCGTCCAC TACTTCAAGG GCTGGTTCCT CATCGACATG GTGGCCGCCA TCCCCTTCGA CCTGCTCATC TTCGGCTCTG GCTCTGAGGA GCTGATCGGG CTGCTGAAGA CTGCGCGGCT GCTGCGGCTG GTGCGCGTGG CGCGGAAGCT GGATCGCTAC TCAGAGTACG GCGCGGCCGT GCTGTTCTTG CTCATGTGCA CCTTTGCGCT CATCGCGCAC TGGCTAGCCT GCATCTGGTA CGCCATCGGC AACATGGAGC AGCCACACAT GGACTCACGC ATCGGCTGGC TGCACAACCT GGGCGACCAG ATAGGCAAAC CCTACAACAG CAGCGGCCTG GGCGGCCCCT CCATCAAGGA CAAGTATGTG ACGGCGCTCT ACTTCACCTT CAGCAGCCTC ACCAGTGTGG GCTTCGGCAA CGTCTCTCCC AACACCAACT CAGAGAAGAT CTTCTCCATC TGCGTCATGC TCATTGGCTC CCTCATGTAT GCTAGCATCT TCGGCAACGT GTCGGCCATC ATCCAGCGGC TGTACTCGGG CACAGCCCGC TACCACACAC AGATGCTGCG GGTGCGGGAG TTCATCCGCT TCCACCAGAT CCCCAATCCC CTGCGCCAGC GCCTCGAGGA GTACTTCCAG CACGCCTGGT CCTACACCAA CGGCATCGAC ATGAACGCGG TGCTGAAGGG CTTCCCTGAG TGCCTGCAGG CTGACATCTG CCTGCACCTG AACCGCTCAC TGCTGCAGCA CTGCAAACCC TTCCGAGGGG CCACCAAGGG CTGCCTTCGG GCCCTGGCCA TGAAGTTCAA GACCACACAT GCACCGCCAG GGGACACACT GGTGCATGCT GGGGACCTGC TCACCGCCCT GTACTTCATC TCCCGGGGCT CCATCGAGAT CCTGCGGGGC GACGTCGTCG TGGCCATCCT GGGGAAGAAT GACATCTTTG GGGAGCCTCT GAACCTGTAT GCAAGGCCTG GCAAGTCGAA CGGGGATGTG CGGGCCCTCA CCTACTGTGA CCTACACAAG ATCCATCGGG ACGACCTGCT GGAGGTGCTG GACATGTACC CTGAGTTCTC CGACCACTTC TGGTCCAGCC TGGAGATCAC CTTCAACCTG CGAGATACCA ACATGATCCC GGGCTCCCCC GGCAGTACGG AGTTAGAGGG TGGCTTCAGT CGGCAACGCA AGCGCAAGTT GTCCTTCCGC AGGCGCACGG ACAAGGACAC GGAGCAGCCA GGGGAGGTGT CGGCCTTGGG GCCGGGCCGG GCGGGGGCAG GGCCGAGTAG CCGGGGCCGG CCGGGGGGGC CGTGGGGGGA GAGCCCGTCC AGTGGCCCCT CCAGCCCTGA GAGCAGTGAG GATGAGGGCC CAGGCCGCAG CTCCAGCCCC CTCCGCCTGG TGCCCTTCTC CAGCCCCAGG CCCCCCGGAG AGCCGCCGGG TGGGGAGCCC CTGATGGAGG ACTGCGAGAA GAGCAGCGAC ACTTGCAACC CCCTGTCAGG CGCCTTCTCA GGAGTGTCCA ACATTTTCAG CTTCTGGGGG GACAGTCGGG GCCGCCAGTA CCAGGAGCTC CCTCGATGCC CCGCCCCCAC CCCCAGCCTC CTCAACATCC CCCTCTCCAG CCCGGGTCGG CGGCCCCGGG GCGACGTGGA GAGCAGGCTG GATGCCCTCC AGCGCCAGCT CAACAGGCTG GAGACCCGGC TGAGTGCAGA CATGGCCACT GTCCTGCAGC TGCTACAGAG GCAGATGACG CTGGTCCCGC CCGCCTACAG TGCTGTGACC ACCCCGGGGC CTGGCCCCAC TTCCACATCC CCGCTGTTGC CCGTCAGCCC CCTCCCCACC CTCACCTTGG ACTCGCTTTC TCAGGTTTCC CAGTTCATGG CGTGTGAGGA GCTGCCCCCG GGGGCCCCAG AGCTTCCCCA AGAAGGCCCC ACACGACGCC TCTCCCTACC GGGCCAGCTG GGGGCCCTCA CCTCCCAGCC CCTGCACAGA CACGGCTCGG ACCCGGGCAG TACGCGTGAC TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC AAGTGAGGAT CCGGGCCCGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGTC TAGAGATATA CTGAGTCATT AGGGACTTTC CAATGGGTTT TGCCCAGTAC ATAAGGTCAA TAGGGGTGAA TCAACAGGAA AGTCCCATTG GAGCCAAGTA CACTGAGTCA ATAGGGACTT TCCATTGGGT TTTGCCCAGT ACAAAAGGTC AATAGGGGGT GAGTCAATGG GTTTTTCCCA TTATTGGCAC GTACATAAGG TCAATAGGGG TGAGTCATTG GGTTTTTCCA GCCAATTTAA TTAAAACGCC ATGTACTTTC CCACCATTGA CGTCAATGGG CTATTGAAAC TAATGCAACG TGACCTTTAA ACGGTACTTT CCCATAGCTG ATTAATGGGA AAGTACCGTT CTCGAGCCAA TACACGTCAA TGGGAAGTGA AAGGGCAGCC AAAACGTAAC ACCGCCCCGG TTTTCCCCTG GAAATTCCAT ATTGGCACGC ATTCTATTGG CTGAGCTGCG TTCTACGTGG GTATAAGAGG CGCGACCAGC GTCGGTACCG TCGCAGTCTT CGGTCTGACC ACCGTAGAAC GCAGATCACC GGTCGCCACC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTAA AGCGGCCGCG ACTCTAGATC ATAATCAGCC ATACCACATT TGTAGAGGTT TTACTTGCTT TAAAAAACCT CCCACACCTC CCCCTGAACC TGAAACATAA AATGAATGCA ATTGTTGTTG TTAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTAAGGCGT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTCCT GAGGCGGAAA GAACCAGCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAGATC GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAAGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGAG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCTAG GGGGAGGCTA ACTGAAACAC GGAAGGAGAC AATACCGGAA GGAACCCGCG CTATGACGGC AATAAAAAGA CAGAATAAAA CGCACGGTGT TGGGTCGTTT GTTCATAAAC GCGGGGTTCG GTCCCAGGGC TGGCACTCTG TCGATACCCC ACCGAGACCC CATTGGGGCC AATACGCCCG CGTTTCTTCC TTTTCCCCAC CCCACCCCCC AAGTTCGGGT GAAGGCCCAG GGCTCGCAGC CAACGTCGGG GCGGCAGGCC CTGCCATAGC CTCAGGTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCA TGCAT
