pEarleyGate103
$84.00
In stock
SKU
PL32675
100μg liquid plasmid(Instantly):$105
| name | pEarleyGate103 | another name | apEarleyGate103 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Protein expression, Gateway | Fragment type | |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | 35S | Replicon | pUC |
| Competence | DB3.1 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | 35S | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGGCAGGATA TATTGTGGTG TAAACAAATT GACGCTTAGA CAACTTAATA ACACATTGCG GACGTTTTTA ATGTACTGAA TTAACGCCGA ATTAATTCGA GCTCGGATCT GATAATTTAT TTGAAAATTC ATAAGAAAAG CAAACGTTAC ATGAATTGAT GAAACAATAC AAAGACAGAT AAAGCCACGC ACATTTAGGA TATTGGCCGA GATTACTGAA TATTGAGTAA GATCACGGAA TTTCTGACAG GAGCATGTCT TCAATTCAGC CCAAATGGCA GTTGAAATAC TCAAACCGCC CCATATGCAG GAGCGGATCA TTCATTGTTT GTTTGGTTGC CTTTGCCAAC ATGGGAGTCC AAGATTCTGC AGTCAAATCT CGGTGACGGG CAGGACCGGA CGGGGCGGTA CCGGCAGGCT GAAGTCCAGC TGCCAGAAAC CCACGTCATG CCAGTTCCCG TGCTTGAAGC CGGCCGCCCG CAGCATGCCG CGGGGGGCAT ATCCGAGCGC CTCGTGCATG CGCACGCTCG GGTCGTTGGG CAGCCCGATG ACAGCGACCA CGCTCTTGAA GCCCTGTGCC TCCAGGGACT TCAGCAGGTG GGTGTAGAGC GTGGAGCCCA GTCCCGTCCG CTGGTGGCGG GGGGAGACGT ACACGGTCGA CTCGGCCGTC CAGTCGTAGG CGTTGCGTGC CTTCCAGGGG CCCGCGTAGG CGATGCCGGC GACCTCGCCG TCCACCTCGG CGACGAGCCA GGGATAGCGC TCCCGCAGAC GGACGAGGTC GTCCGTCCAC TCCTGCGGTT CCTGCGGCTC GGTACGGAAG TTGACCGTGC TTGTCTCGAT GTAGTGGTTG ACGATGGTGC AGACCGCCGG CATGTCCGCC TCGGTGGCAC GGCGGATGTC GGCCGGGCGT CGTTCTGGGC TCATCGATTC GATTTGGTGT ATCGAGATTG GTTATGAAAT TCAGATGCTA GTGTAATGTA TTGGTAATTT GGGAAGATAT AATAGGAAGC AAGGCTATTT ATCCATTTCT GAAAAGGCGA AATGGCGTCA CCGCGAGCGT CACGCGCATT CCGTTCTTGC TGTAAAGCGT TGTTTGGTAC ACTTTTGACT AGCGAGGCTT GGCGTGTCAG CGTATCTATT CAAAAGTCGT TAATGGCTGC GGATCAAGAA AAAGTTGGAA TAGAAACAGA ATACCCGCGA AATTCAGGCC CGGTTGCCAT GTCCTACACG CCGAAATAAA CGACCAAATT AGTAGAAAAA TAAAAACTGA CTCGGATACT TACGTCACGT CTTGCGCACT GATTTGAAAA ATCTCAGAAT TCCAATCCCA CAAAAATCTG AGCTTAACAG CACAGTTGCT CCTCTCAGAG CAGAATCGGG TATTCAACAC CCTCATATCA ACTACTACGT TGTGTATAAC GGTCCACATG CCGGTATATA CGATGACTGG GGTTGTACAA AGGCGGCAAC AAACGGCGTT CCCGGAGTTG CACACAAGAA ATTTGCCACT ATTACAGAGG CAAGAGCAGC AGCTGACGCG TACACAACAA GTCAGCAAAC AGACAGGTTG AACTTCATCC CCAAAGGAGA AGCTCAACTC AAGCCCAAGA GCTTTGCTAA GGCCCTAACA AGCCCACCAA AGCAAAAAGC CCACTGGCTC ACGCTAGGAA CCAAAAGGCC CAGCAGTGAT CCAGCCCCAA AAGAGATCTC CTTTGCCCCG GAGATTACAA TGGACGATTT CCTCTATCTT TACGATCTAG GAAGGAAGTT CGAAGGTGAA GGTGACGACA CTATGTTCAC CACTGATAAT GAGAAGGTTA GCCTCTTCAA TTTCAGAAAG AATGCTGACC CACAGATGGT TAGAGAGGCC TACGCAGCAG GTCTCATCAA GACGATCTAC CCGAGTAACA ATCTCCAGGA GATCAAATAC CTTCCCAAGA AGGTTAAAGA TGCAGTCAAA AGATTCAGGA CTAATTGCAT CAAGAACACA GAGAAAGACA TATTTCTCAA GATCAGAAGT ACTATTCCAG TATGGACGAT TCAAGGCTTG CTTCATAAAC CAAGGCAAGT AATAGAGATT GGAGTCTCTA AAAAGGTAGT TCCTACTGAA TCTAAGGCCA TGCATGGAGT CTAAGATTCA AATCGAGGAT CTAACAGAAC TCGCCGTGAA GACTGGCGAA CAGTTCATAC AGAGTCTTTT ACGACTCAAT GACAAGAAGA AAATCTTCGT CAACATGGTG GAGCACGACA CTCTGGTCTA CTCCAAAAAT GTCAAAGATA CAGTCTCAGA AGACCAAAGG GCTATTGAGA CTTTTCAACA AAGGATAATT TCGGGAAACC TCCTCGGATT CCATTGCCCA GCTATCTGTC ACTTCATCGA AAGGACAGTA GAAAAGGAAG GTGGCTCCTA CAAATGCCAT CATTGCGATA AAGGAAAGGC TATCATTCAA GATCTCTCTG CCGACAGTGG TCCCAAAGAT GGACCCCCAC CCACGAGGAG CATCGTGGAA AAAGAAGACG TTCCAACCAC GTCTTCAAAG CAAGTGGATT GATGTGACAT CTCCACTGAC GTAAGGGATG ACGCACAATC CCACTATCCT TCGCAAGACC CTTCCTCTAT ATAAGGAAGT TCATTTCATT TGGAGAGGAC ACGCTCGAGA TCACAAGTTT GTACAAAAAA GCTGAACGAG AAACGTAAAA TGATATAAAT ATCAATATAT TAAATTAGAT TTTGCATAAA AAACAGACTA CATAATACTG TAAAACACAA CATATCCAGT CATATTGGCG GCCGCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATAA TGTGTGGATT TTGAGTTAGG ATCCGTCGAG ATTTTCAGGA GCTAAGGAAG CTAAAATGGA GAAAAAAATC ACTGGATATA CCACCGTTGA TATATCCCAA TGGCATCGTA AAGAACATTT TGAGGCATTT CAGTCAGTTG CTCAATGTAC CTATAACCAG ACCGTTCAGC TGGATATTAC GGCCTTTTTA AAGACCGTAA AGAAAAATAA GCACAAGTTT TATCCGGCCT TTATTCACAT TCTTGCCCGC CTGATGAATG CTCATCCGGA ATTCCGTATG GCAATGAAAG ACGGTGAGCT GGTGATATGG GATAGTGTTC ACCCTTGTTA CACCGTTTTC CATGAGCAAA CTGAAACGTT TTCATCGCTC TGGAGTGAAT ACCACGACGA TTTCCGGCAG TTTCTACACA TATATTCGCA AGATGTGGCG TGTTACGGTG AAAACCTGGC CTATTTCCCT AAAGGGTTTA TTGAGAATAT GTTTTTCGTC TCAGCCAATC CCTGGGTGAG TTTCACCAGT TTTGATTTAA ACGTGGCCAA TATGGACAAC TTCTTCGCCC CCGTTTTCAC CATGGGCAAA TATTATACGC AAGGCGACAA GGTGCTGATG CCGCTGGCGA TTCAGGTTCA TCATGCCGTT TGTGATGGCT TCCATGTCGG CAGAATGCTT AATGAATTAC AACAGTACTG CGATGAGTGG CAGGGCGGGG CGTAAACGCG TGGATCCGGC TTACTAAAAG CCAGATAACA GTATGCGTAT TTGCGCGCTG ATTTTTGCGG TATAAGAATA TATACTGATA TGTATACCCG AAGTATGTCA AAAAGAGGTA TGCTATGAAG CAGCGTATTA CAGTGACAGT TGACAGCGAC AGCTATCAGT TGCTCAAGGC ATATATGATG TCAATATCTC CGGTCTGGTA AGCACAACCA TGCAGAATGA AGCCCGTCGT CTGCGTGCCG AACGCTGGAA AGCGGAAAAT CAGGAAGGGA TGGCTGAGGT CGCCCGGTTT ATTGAAATGA ACGGCTCTTT TGCTGACGAG AACAGGGGCT GGTGAAATGC AGTTTAAGGT TTACACCTAT AAAAGAGAGA GCCGTTATCG TCTGTTTGTG GATGTACAGA GTGATATTAT TGACACGCCC GGGCGACGGA TGGTGATCCC CCTGGCCAGT GCACGTCTGC TGTCAGATAA AGTCTCCCGT GAACTTTACC CGGTGGTGCA TATCGGGGAT GAAAGCTGGC GCATGATGAC CACCGATATG GCCAGTGTGC CGGTCTCCGT TATCGGGGAA GAAGTGGCTG ATCTCAGCCA CCGCGAAAAT GACATCAAAA ACGCCATTAA CCTGATGTTC TGGGGAATAT AAATGTCAGG CTCCCTTATA CACAGCCAGT CTGCAGGTCG ACCATAGTGA CTGGATATGT TGTGTTTTAC AGCATTATGT AGTCTGTTTT TTATGCAAAA TCTAATTTAA TATATTGATA TTTATATCAT TTTACGTTTC TCGTTCAGCT TTCTTGTACA AAGTGGTGCT CGAGATGGTA GATCTGACTA GTAAAGGAGA AGAACTTTTC ACTGGAGTTG TCCCAATTCT TGTTGAATTA GATGGTGATG TTAATGGGCA CAAATTTTCT GTCAGTGGAG AGGGTGAAGG TGATGCAACA TACGGAAAAC TTACCCTTAA ATTTATTTGC ACTACTGGAA AACTACCTGT TCCGTGGCCA ACACTTGTCA CTACTTTCTC TTATGGTGTT CAATGCTTTT CAAGATACCC AGATCATATG AAGCGGCACG ACTTCTTCAA GAGCGCCATG CCTGAGGGAT ACGTGCAGGA GAGGACCATC TTCTTCAAGG ACGACGGGAA CTACAAGACA CGTGCTGAAG TCAAGTTTGA GGGAGACACC CTCGTCAACA GGATCGAGCT TAAGGGAATC GATTTCAAGG AGGACGGAAA CATCCTCGGC CACAAGTTGG AATACAACTA CAACTCCCAC AACGTATACA TCATGGCCGA CAAGCAAAAG AACGGCATCA AAGCCAACTT CAAGACCCGC CACAACATCG AAGACGGCGG CGTGCAACTC GCTGATCATT ATCAACAAAA TACTCCAATT GGCGATGGCC CTGTCCTTTT ACCAGACAAC CATTACCTGT CCACACAATC TGCCCTTTCG AAAGATCCCA ACGAAAAGAG AGACCACATG GTCCTTCTTG AGTTTGTAAC AGCTGCTGGG ATTACACATG GCATGGATGA ACTATACAAA GCTAGCCACC ACCACCACCA CCACGTGTGA CCTAGGTGAG TCTAGAGAGT TAATTAAGAC CCGGGACTAG TCCCTAGAGT CCTGCTTTAA TGAGATATGC GAGACGCCTA TGATCGCATG ATATTTGCTT TCAATTCTGT TGTGCACGTT GTAAAAAACC TGAGCATGTG TAGCTCAGAT CCTTACCGCC GGTTTCGGTT CATTCTAATG AATATATCAC CCGTTACTAT CGTATTTTTA TGAATAATAT TCTCCGTTCA ATTTACTGAT TGTACCCTAC TACTTATATG TACAATATTA AAATGAAAAC AATATATTGT GCTGAATAGG TTTATAGCGA CATCTATGAT AGAGCGCCAC AATAACAAAC AATTGCGTTT TATTATTACA AATCCAATTT TAAAAAAAGC GGCAGAACCG GTCAAACCTA AAAGACTGAT TACATAAATC TTATTCAAAT TTCAAAAGTG CCCCAGGGGC TAGTATCTAC GACACACCGA GCGGCGAACT AATAACGCTC ACTGAAGGGA ACTCCGGTTC CCCGCCGGCG CGCATGGGTG AGATTCCTTG AAGTTGAGTA TTGGCCGTCC GCTCTACCGA AAGTTACGGG CACCATTCAA CCCGGTCCAG CACGGCGGCC GGGTAACCGA CTTGCTGCCC CGAGAATTAT GCAGCATTTT TTTGGTGTAT GTGGGCCCCA AATGAAGTGC AGGTCAAACC TTGACAGTGA CGACAAATCG TTGGGCGGGT CCAGGGCGAA TTTTGCGACA ACATGTCGAG GCTCAGCAGG ACCTGCAGGC ATGCAAGCTT GGCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGC TAGAGCAGCT TGAGCTTGGA TCAGATTGTC GTTTCCCGCC TTCAGTTTAA ACTATCAGTG TTTGACAGGA TATATTGGCG GGTAAACCTA AGAGAAAAGA GCGTTTATTA GAATAACGGA TATTTAAAAG GGCGTGAAAA GGTTTATCCG TTCGTCCATT TGTATGTGCA TGCCAACCAC AGGGTTCCCC TCGGGATCAA AGTACTTTGA TCCAACCCCT CCGCTGCTAT AGTGCAGTCG GCTTCTGACG TTCAGTGCAG CCGTCTTCTG AAAACGACAT GTCGCACAAG TCCTAAGTTA CGCGACAGGC TGCCGCCCTG CCCTTTTCCT GGCGTTTTCT TGTCGCGTGT TTTAGTCGCA TAAAGTAGAA TACTTGCGAC TAGAACCGGA GACATTACGC CATGAACAAG AGCGCCGCCG CTGGCCTGCT GGGCTATGCC CGCGTCAGCA CCGACGACCA GGACTTGACC AACCAACGGG CCGAACTGCA CGCGGCCGGC TGCACCAAGC TGTTTTCCGA GAAGATCACC GGCACCAGGC GCGACCGCCC GGAGCTGGCC AGGATGCTTG ACCACCTACG CCCTGGCGAC GTTGTGACAG TGACCAGGCT AGACCGCCTG GCCCGCAGCA CCCGCGACCT ACTGGACATT GCCGAGCGCA TCCAGGAGGC CGGCGCGGGC CTGCGTAGCC TGGCAGAGCC GTGGGCCGAC ACCACCACGC CGGCCGGCCG CATGGTGTTG ACCGTGTTCG CCGGCATTGC CGAGTTCGAG CGTTCCCTAA TCATCGACCG CACCCGGAGC GGGCGCGAGG CCGCCAAGGC CCGAGGCGTG AAGTTTGGCC CCCGCCCTAC CCTCACCCCG GCACAGATCG CGCACGCCCG CGAGCTGATC GACCAGGAAG GCCGCACCGT GAAAGAGGCG GCTGCACTGC TTGGCGTGCA TCGCTCGACC CTGTACCGCG CACTTGAGCG CAGCGAGGAA GTGACGCCCA CCGAGGCCAG GCGGCGCGGT GCCTTCCGTG AGGACGCATT GACCGAGGCC GACGCCCTGG CGGCCGCCGA GAATGAACGC CAAGAGGAAC AAGCATGAAA CCGCACCAGG ACGGCCAGGA CGAACCGTTT TTCATTACCG AAGAGATCGA GGCGGAGATG ATCGCGGCCG GGTACGTGTT CGAGCCGCCC GCGCACGTCT CAACCGTGCG GCTGCATGAA ATCCTGGCCG GTTTGTCTGA TGCCAAGCTG GCGGCCTGGC CGGCCAGCTT GGCCGCTGAA GAAACCGAGC GCCGCCGTCT AAAAAGGTGA TGTGTATTTG AGTAAAACAG CTTGCGTCAT GCGGTCGCTG CGTATATGAT GCGATGAGTA AATAAACAAA TACGCAAGGG GAACGCATGA AGGTTATCGC TGTACTTAAC CAGAAAGGCG GGTCAGGCAA GACGACCATC GCAACCCATC TAGCCCGCGC CCTGCAACTC GCCGGGGCCG ATGTTCTGTT AGTCGATTCC GATCCCCAGG GCAGTGCCCG CGATTGGGCG GCCGTGCGGG AAGATCAACC GCTAACCGTT GTCGGCATCG ACCGCCCGAC GATTGACCGC GACGTGAAGG CCATCGGCCG GCGCGACTTC GTAGTGATCG ACGGAGCGCC CCAGGCGGCG GACTTGGCTG TGTCCGCGAT CAAGGCAGCC GACTTCGTGC TGATTCCGGT GCAGCCAAGC CCTTACGACA TATGGGCCAC CGCCGACCTG GTGGAGCTGG TTAAGCAGCG CATTGAGGTC ACGGATGGAA GGCTACAAGC GGCCTTTGTC GTGTCGCGGG CGATCAAAGG CACGCGCATC GGCGGTGAGG TTGCCGAGGC GCTGGCCGGG TACGAGCTGC CCATTCTTGA GTCCCGTATC ACGCAGCGCG TGAGCTACCC AGGCACTGCC GCCGCCGGCA CAACCGTTCT TGAATCAGAA CCCGAGGGCG ACGCTGCCCG CGAGGTCCAG GCGCTGGCCG CTGAAATTAA ATCAAAACTC ATTTGAGTTA ATGAGGTAAA GAGAAAATGA GCAAAAGCAC AAACACGCTA AGTGCCGGCC GTCCGAGCGC ACGCAGCAGC AAGGCTGCAA CGTTGGCCAG CCTGGCAGAC ACGCCAGCCA TGAAGCGGGT CAACTTTCAG TTGCCGGCGG AGGATCACAC CAAGCTGAAG ATGTACGCGG TACGCCAAGG CAAGACCATT ACCGAGCTGC TATCTGAATA CATCGCGCAG CTACCAGAGT AAATGAGCAA ATGAATAAAT GAGTAGATGA ATTTTAGCGG CTAAAGGAGG CGGCATGGAA AATCAAGAAC AACCAGGCAC CGACGCCGTG GAATGCCCCA TGTGTGGAGG AACGGGCGGT TGGCCAGGCG TAAGCGGCTG GGTTGTCTGC CGGCCCTGCA ATGGCACTGG AACCCCCAAG CCCGAGGAAT CGGCGTGAGC GGTCGCAAAC CATCCGGCCC GGTACAAATC GGCGCGGCGC TGGGTGATGA CCTGGTGGAG AAGTTGAAGG CCGCGCAGGC CGCCCAGCGG CAACGCATCG AGGCAGAAGC ACGCCCCGGT GAATCGTGGC AAGCGGCCGC TGATCGAATC CGCAAAGAAT CCCGGCAACC GCCGGCAGCC GGTGCGCCGT CGATTAGGAA GCCGCCCAAG GGCGACGAGC AACCAGATTT TTTCGTTCCG ATGCTCTATG ACGTGGGCAC CCGCGATAGT CGCAGCATCA TGGACGTGGC CGTTTTCCGT CTGTCGAAGC GTGACCGACG AGCTGGCGAG GTGATCCGCT ACGAGCTTCC AGACGGGCAC GTAGAGGTTT CCGCAGGGCC GGCCGGCATG GCCAGTGTGT GGGATTACGA CCTGGTACTG ATGGCGGTTT CCCATCTAAC CGAATCCATG AACCGATACC GGGAAGGGAA GGGAGACAAG CCCGGCCGCG TGTTCCGTCC ACACGTTGCG GACGTACTCA AGTTCTGCCG GCGAGCCGAT GGCGGAAAGC AGAAAGACGA CCTGGTAGAA ACCTGCATTC GGTTAAACAC CACGCACGTT GCCATGCAGC GTACGAAGAA GGCCAAGAAC GGCCGCCTGG TGACGGTATC CGAGGGTGAA GCCTTGATTA GCCGCTACAA GATCGTAAAG AGCGAAACCG GGCGGCCGGA GTACATCGAG ATCGAGCTAG CTGATTGGAT GTACCGCGAG ATCACAGAAG GCAAGAACCC GGACGTGCTG ACGGTTCACC CCGATTACTT TTTGATCGAT CCCGGCATCG GCCGTTTTCT CTACCGCCTG GCACGCCGCG CCGCAGGCAA GGCAGAAGCC AGATGGTTGT TCAAGACGAT CTACGAACGC AGTGGCAGCG CCGGAGAGTT CAAGAAGTTC TGTTTCACCG TGCGCAAGCT GATCGGGTCA AATGACCTGC CGGAGTACGA TTTGAAGGAG GAGGCGGGGC AGGCTGGCCC GATCCTAGTC ATGCGCTACC GCAACCTGAT CGAGGGCGAA GCATCCGCCG GTTCCTAATG TACGGAGCAG ATGCTAGGGC AAATTGCCCT AGCAGGGGAA AAAGGTCGAA AAGGTCTCTT TCCTGTGGAT AGCACGTACA TTGGGAACCC AAAGCCGTAC ATTGGGAACC GGAACCCGTA CATTGGGAAC CCAAAGCCGT ACATTGGGAA CCGGTCACAC ATGTAAGTGA CTGATATAAA AGAGAAAAAA GGCGATTTTT CCGCCTAAAA CTCTTTAAAA CTTATTAAAA CTCTTAAAAC CCGCCTGGCC TGTGCATAAC TGTCTGGCCA GCGCACAGCC GAAGAGCTGC AAAAAGCGCC TACCCTTCGG TCGCTGCGCT CCCTACGCCC CGCCGCTTCG CGTCGGCCTA TCGCGGCCGC TGGCCGCTCA AAAATGGCTG GCCTACGGCC AGGCAATCTA CCAGGGCGCG GACAAGCCGC GCCGTCGCCA CTCGACCGCC GGCGCCCACA TCAAGGCACC CTGCCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCGCAGCCAT GACCCAGTCA CGTAGCGATA GCGGAGTGTA TACTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GCATTCTAGG TACTAAAACA ATTCATCCAG TAAAATATAA TATTTTATTT TCTCCCAATC AGGCTTGATC CCCAGTAAGT CAAAAAATAG CTCGACATAC TGTTCTTCCC CGATATCCTC CCTGATCGAC CGGACGCAGA AGGCAATGTC ATACCACTTG TCCGCCCTGC CGCTTCTCCC AAGATCAATA AAGCCACTTA CTTTGCCATC TTTCACAAAG ATGTTGCTGT CTCCCAGGTC GCCGTGGGAA AAGACAAGTT CCTCTTCGGG CTTTTCCGTC TTTAAAAAAT CATACAGCTC GCGCGGATCT TTAAATGGAG TGTCTTCTTC CCAGTTTTCG CAATCCACAT CGGCCAGATC GTTATTCAGT AAGTAATCCA ATTCGGCTAA GCGGCTGTCT AAGCTATTCG TATAGGGACA ATCCGATATG TCGATGGAGT GAAAGAGCCT GATGCACTCC GCATACAGCT CGATAATCTT TTCAGGGCTT TGTTCATCTT CATACTCTTC CGAGCAAAGG ACGCCATCGG CCTCACTCAT GAGCAGATTG CTCCAGCCAT CATGCCGTTC AAAGTGCAGG ACCTTTGGAA CAGGCAGCTT TCCTTCCAGC CATAGCATCA TGTCCTTTTC CCGTTCCACA TCATAGGTGG TCCCTTTATA CCGGCTGTCC GTCATTTTTA AATATAGGTT TTCATTTTCT CCCACCAGCT TATATACCTT AGCAGGAGAC ATTCCTTCCG TATCTTTTAC GCAGCGGTAT TTTTCGATCA GTTTTTTCAA TTCCGGTGAT ATTCTCATTT TAGCCATTTA TTATTTCCTT CCTCTTTTCT ACAGTATTTA AAGATACCCC AAGAAGCTAA TTATAACAAG ACGAACTCCA ATTCACTGTT CCTTGCATTC TAAAACCTTA AATACCAGAA AACAGCTTTT TCAAAGTTGT TTTCAAAGTT GGCGTATAAC ATAGTATCGA CGGAGCCGAT TTTGAAACCG CGGTGATCAC AGGCAGCAAC GCTCTGTCAT CGTTACAATC AACATGCTAC CCTCCGCGAG ATCATCCGTG TTTCAAACCC GGCAGCTTAG TTGCCGTTCT TCCGAATAGC ATCGGTAACA TGAGCAAAGT CTGCCGCCTT ACAACGGCTC TCCCGCTGAC GCCGTCCCGG ACTGATGGGC TGCCTGTATC GAGTGGTGAT TTTGTGCCGA GCTGCCGGTC GGGGAGCTGT TGGCTGGCTG G
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