NM_001135191.1;AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha;SHAG1;
100μg liquid plasmid(Instantly):$105
| name | pCMV-ASAP2(human)-mCherry-Neo | another name | apCMV-ASAP2(human)-mCherry-Neo |
| Insert Size (bp) | 2883bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | Red |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGACTCAG ATCTCGAGCT CAAGCTTCGA ATTCGCCACC ATGCCGGACC AGATCTCCGT GTCGGAATTC GTGGCCGAGA CCCATGAGGA CTACAAGGCG CCCACGGCCT CCAGCTTCAC CACCCGCACG GCGCAGTGCC GGAACACTGT GGCGGCCATC GAGGAGGCTT TGGACGTGGA CCGGATGGTT CTTTACAAAA TGAAGAAATC CGTGAAAGCA ATCAACAGCT CTGGGCTGGC TCACGTGGAA AATGAAGAGC AGTACACCCA GGCTCTGGAG AAGTTTGGCG GCAACTGTGT ATGCAGAGAT GACCCAGATT TAGGAAGTGC GTTCCTGAAG TTCTCAGTGT TTACAAAGGA GTTGACAGCA CTTTTCAAAA ACCTGATTCA GAATATGAAC AACATAATCT CCTTCCCTTT GGACAGTTTG CTGAAGGGGG ACCTGAAAGG AGTGAAAGGG GATCTGAAAA AGCCTTTTGA TAAAGCTTGG AAGGACTATG AAACAAAAAT AACCAAGATA GAAAAGGAGA AAAAGGAACA CGCCAAGCTC CATGGGATGA TTCGGACTGA AATAAGCGGA GCGGAAATTG CCGAAGAGAT GGAAAAGGAG AGGCGCTTCT TCCAGCTACA GATGTGCGAG TATCTGCTGA AGGTCAACGA AATCAAGATT AAAAAGGGAG TAGATTTACT TCAGAATCTG ATCAAATACT TTCATGCCCA ATGCAATTTT TTTCAGGATG GACTCAAAGC CGTGGAAAGC CTCAAACCTT CCATTGAAAC GCTGTCTACG GATCTTCACA CGATCAAACA GGCCCAGGAT GAAGAAAGAA GGCAGTTGAT ACAGCTTCGA GATATTTTGA AATCCGCATT GCAGGTTGAA CAGAAAGAGG ACTCCCAAAT TCGTCAGAGC ACAGCTTATA GCTTACATCA GCCTCAGGGA AACAAGGAAC ATGGGACCGA GCGGAACGGC AGCCTCTACA AGAAGAGTGA CGGGATCCGA AAAGTGTGGC AGAAAAGGAA ATGTTCAGTT AAAAATGGTT TTCTGACCAT ATCCCATGGT ACCGCTAACC GGCCTCCTGC AAAGCTCAAC CTGCTAACCT GCCAGGTGAA GACCAACCCT GAGGAGAAGA AGTGCTTTGA CCTCATTTCA CATGACAGAA CTTACCACTT TCAAGCTGAA GATGAACAGG AATGTCAAAT ATGGATGTCT GTGCTGCAAA ATAGCAAAGA AGAAGCTTTA AACAATGCAT TTAAGGGGGA TGACAATACT GGAGAAAATA ACATCGTCCA AGAACTGACA AAGGAGATCA TCTCAGAAGT GCAGAGGATG ACGGGCAATG ACGTCTGCTG TGACTGTGGG GCGCCAGATC CTACATGGCT TTCCACCAAC CTGGGCATCC TGACCTGCAT CGAGTGTTCC GGAATCCACC GAGAGCTGGG GGTTCATTAT TCCAGGATGC AGTCCCTGAC CTTAGATGTA CTGGGAACAT CTGAGCTGCT GCTCGCCAAG AATATTGGGA ATGCAGGCTT TAATGAGATC ATGGAATGTT GCCTACCAGC TGAGGACTCA GTCAAACCCA ACCCAGGCAG CGACATGAAT GCAAGAAAGG ACTACATCAC AGCCAAGTAC ATCGAGAGGA GATACGCAAG GAAGAAGCAC GCGGATAACG CGGCGAAGCT TCACAGTCTT TGCGAGGCCG TCAAAACGAG AGATATTTTT GGATTGCTCC AAGCTTATGC TGATGGTGTG GATCTTACGG AAAAAATCCC ACTGGCCAAC GGACATGAGC CGGATGAAAC GGCCCTCCAC CTTGCAGTCA GATCCGTGGA TCGAACCTCT CTTCACATTG TAGACTTTTT AGTTCAGAAC AGTGGGAACC TGGATAAACA GACAGGGAAA GGCAGCACAG CCCTGCACTA CTGCTGCCTG ACCGACAATG CCGAGTGCCT CAAGTTGCTC CTGCGGGGGA AGGCCTCCAT CGAGATAGCA AACGAGTCAG GAGAGACTCC GCTGGACATT GCCAAGCGCC TCAAGCACGA GCACTGTGAG GAGCTGCTGA CCCAAGCCTT ATCTGGAAGA TTTAATTCTC ACGTTCACGT TGAATATGAA TGGCGACTAC TCCACGAAGA CCTGGATGAA AGTGATGACG ACATGGATGA GAAATTGCAG CCCAGTCCCA ACCGGCGGGA AGACCGGCCC ATCAGCTTCT ACCAGCTGGG CTCCAACCAG CTTCAGTCTA ACGCTGTATC TTTGGCCAGA GATGCTGCAA ACCTTGCCAA GGAGAAGCAG AGGGCTTTCA TGCCCAGCAT CTTGCAGAAT GAGACTTACG GAGCCCTCCT GAGTGGCAGC CCACCTCCCG CCCAGCCTGC AGCCCCCAGC ACCACCAGCG CCCCCCCGCT TCCTCCACGG AATGTTGGCA AAGATCCCCT GACCCCCACG CCGCCCCCAC CCGTTGCCAA GACGCCCAGC GTAATGGAAG CCTTGAGCCA GCCGAGCAAG CCTGCCCCGC CTGGGATCTC ACAGATCAGG CCCCCACCTC TGCCCCCACA GCCGCCCAGC CGCCTCCCGC AGAAGAAGCC TGCGCCGGGG GCTGACAAGT CCACCCCACT GACCAACAAA GGCCAACCGA GAGGACCTGT GGATCTCTCT GCAACGGAAG CTCTGGGTCC TCTGTCCAAT GCTATGGTCC TGCAGCCCCC TGCACCCATG CCTAGGAAGT CGCAGGCAAC CAAGTTGAAG CCTAAGCGGG TGAAAGCGCT CTATAACTGT GTGGCTGACA ACCCCGATGA GCTCACCTTC TCCGAGGGGG ATGTGATCAT CGTGGACGGG GAGGAGGACC AGGAGTGGTG GATTGGCCAC ATTGATGGAG ATCCTGGTCG CAAAGGCGCA TTCCCGGTGT CATTTGTGCA CTTTATCGCT GACGGTGGAG GCGGATCAGG AGGCGGTGGA TCTTCGGATC CACCGGTCGC CACCATGGTG AGCAAGGGCG AGGAGGATAA CATGGCCATC ATCAAGGAGT TCATGCGCTT CAAGGTGCAC ATGGAGGGCT CCGTGAACGG CCACGAGTTC GAGATCGAGG GCGAGGGCGA GGGCCGCCCC TACGAGGGCA CCCAGACCGC CAAGCTGAAG GTGACCAAGG GTGGCCCCCT GCCCTTCGCC TGGGACATCC TGTCCCCTCA GTTCATGTAC GGCTCCAAGG CCTACGTGAA GCACCCCGCC GACATCCCCG ACTACTTGAA GCTGTCCTTC CCCGAGGGCT TCAAGTGGGA GCGCGTGATG AACTTCGAGG ACGGCGGCGT GGTGACCGTG ACCCAGGACT CCTCCCTGCA GGACGGCGAG TTCATCTACA AGGTGAAGCT GCGCGGCACC AACTTCCCCT CCGACGGCCC CGTAATGCAG AAGAAGACCA TGGGCTGGGA GGCCTCCTCC GAGCGGATGT ACCCCGAGGA CGGCGCCCTG AAGGGCGAGA TCAAGCAGAG GCTGAAGCTG AAGGACGGCG GCCACTACGA CGCTGAGGTC AAGACCACCT ACAAGGCCAA GAAGCCCGTG CAGCTGCCCG GCGCCTACAA CGTCAACATC AAGTTGGACA TCACCTCCCA CAACGAGGAC TACACCATCG TGGAACAGTA CGAACGCGCC GAGGGCCGCC ACTCCACCGG CGGCATGGAC GAGCTGTACA AGTAGCGGCC GCGACTCTAG ATCATAATCA GCCATACCAC ATTTGTAGAG GTTTTACTTG CTTTAAAAAA CCTCCCACAC CTCCCCCTGA ACCTGAAACA TAAAATGAAT GCAATTGTTG TTGTTAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTAAGG CGTAAATTGT AAGCGTTAAT ATTTTGTTAA AATTCGCGTT AAATTTTTGT TAAATCAGCT CATTTTTTAA CCAATAGGCC GAAATCGGCA AAATCCCTTA TAAATCAAAA GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA ACCGTCTATC AGGGCGATGG CCCACTACGT GAACCATCAC CCTAATCAAG TTTTTTGGGG TCGAGGTGCC GTAAAGCACT AAATCGGAAC CCTAAAGGGA GCCCCCGATT TAGAGCTTGA CGGGGAAAGC CGGCGAACGT GGCGAGAAAG GAAGGGAAGA AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC GGTCACGCTG CGCGTAACCA CCACACCCGC CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT CCTGAGGCGG AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAG ATCGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC TAGGGGGAGG CTAACTGAAA CACGGAAGGA GACAATACCG GAAGGAACCC GCGCTATGAC GGCAATAAAA AGACAGAATA AAACGCACGG TGTTGGGTCG TTTGTTCATA AACGCGGGGT TCGGTCCCAG GGCTGGCACT CTGTCGATAC CCCACCGAGA CCCCATTGGG GCCAATACGC CCGCGTTTCT TCCTTTTCCC CACCCCACCC CCCAAGTTCG GGTGAAGGCC CAGGGCTCGC AGCCAACGTC GGGGCGGCAG GCCCTGCCAT AGCCTCAGGT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCATGCAT
