pCMV-SMC2(human)-EGFP-Neo
$99.00
In stock
SKU
PL32600
NM_001042550.2,SMC2/CAP-E/CAPE/SMC-2/SMC2L1
100μg liquid plasmid(Instantly):$120
| name | pCMV-SMC2(human)-EGFP-Neo | another name | apCMV-SMC2(human)-EGFP-Neo |
| Insert Size (bp) | 3591bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CGACGGATCG GGAGATCTCC CGATCCCCTA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TCTGCTCCCT GCTTGTGTGT TGGAGGTCGC TGAGTAGTGC GCGAGCAAAA TTTAAGCTAC AACAAGGCAA GGCTTGACCG ACAATTGCAT GAAGAATCTG CTTAGGGTTA GGCGTTTTGC GCTGCTTCGC GATGTACGGG CCAGATATAC GCGTTGACAT TGATTATTGA CTAGTTATTA ATAGTAATCA ATTACGGGGT CATTAGTTCA TAGCCCATAT ATGGAGTTCC GCGTTACATA ACTTACGGTA AATGGCCCGC CTGGCTGACC GCCCAACGAC CCCCGCCCAT TGACGTCAAT AATGACGTAT GTTCCCATAG TAACGCCAAT AGGGACTTTC CATTGACGTC AATGGGTGGA GTATTTACGG TAAACTGCCC ACTTGGCAGT ACATCAAGTG TATCATATGC CAAGTACGCC CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT TATGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC ATCGCTATTA CCATGGTGAT GCGGTTTTGG CAGTACATCA ATGGGCGTGG ATAGCGGTTT GACTCACGGG GATTTCCAAG TCTCCACCCC ATTGACGTCA ATGGGAGTTT GTTTTGGCAC CAAAATCAAC GGGACTTTCC AAAATGTCGT AACAACTCCG CCCCATTGAC GCAAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTGGT ACCGAGCTCG GATCCACTAG TCCAGTGTGG TGGAATTCGC CACCATGCAT ATTAAGTCAA TTATTCTAGA GGGATTCAAG TCCTATGCTC AGAGGACCGA AGTCAATGGT TTTGACCCCC TCTTCAATGC TATCACTGGC TTAAATGGTA GTGGGAAATC CAACATATTG GACTCCATCT GCTTTTTGCT GGGCATCTCC AACCTGTCTC AGGTTCGGGC TTCTAATTTA CAAGATTTAG TTTACAAAAA TGGGCAGGCT GGTATTACCA AAGCCTCTGT GTCAATCACT TTTGATAATT CTGACAAAAA GCAAAGTCCT TTAGGATTTG AGGTTCATGA TGAAATCACA GTAACAAGGC AGGTGGTTAT TGGTGGTAGA AATAAATATT TAATCAATGG AGTCAATGCC AACAACACCA GAGTACAGGA TCTCTTCTGT TCTGTTGGCC TTAATGTTAA CAACCCTCAC TTTCTCATCA TGCAGGGCCG AATTACAAAA GTATTGAATA TGAAACCTCC AGAGATTTTA TCCATGATAG AAGAAGCAGC TGGAACCAGG ATGTATGAAT ACAAAAAAAT AGCTGCACAG AAAACTATAG AAAAAAAGGA GGCTAAGCTG AAAGAAATTA AGACGATACT TGAAGAAGAG ATTACTCCAA CCATTCAAAA ATTAAAAGAG GAAAGATCGT CCTACTTGGA GTACCAAAAA GTAATGAGAG AAATAGAACA TTTGAGTCGT TTATATATTG CTTATCAGTT TTTGCTGGCT GAAGATACCA AAGTACGCTC AGCTGAGGAA TTAAAAGAAA TGCAAGATAA AGTTATAAAG CTTCAGGAAG AATTGTCTGA GAATGATAAA AAAATAAAAG CACTTAATCA TGAAATAGAA GAATTGGAAA AAAGAAAAGA TAAGGAAACT GGAGGTATAC TTCGATCTTT AGAAGATGCT CTTGCAGAGG CTCAGCGAGT TAATACTAAA TCTCAAAGCG CATTTGATCT CAAGAAGAAA AATCTGGCAT GTGAGGAAAG CAAACGCAAA GAGCTGGAAA AAAATATGGT TGAGGACTCA AAAACTTTAG CAGCAAAGGA AAAAGAGGTT AAAAAGATAA CAGATGGACT GCATGCCCTT CAAGAAGCAA GTAATAAAGA TGCTGAAGCT CTGGCAGCTG CACAGCAGCA CTTCAATGCT GTTTCCGCTG GCCTGTCCAG TAATGAAGAT GGAGCAGAAG CAACTCTTGC TGGTCAAATG ATGGCCTGTA AAAATGATAT AAGTAAAGCT CAGACAGAAG CCAAACAGGC TCAGATGAAG TTGAAGCATG CTCAACAGGA ATTAAAGAAT AAACAAGCTG AAGTTAAGAA GATGGATAGT GGCTACAGGA AGGATCAAGA AGCTCTAGAA GCTGTAAAAA GACTTAAAGA AAAACTTGAA GCTGAAATGA AAAAGCTAAA TTATGAAGAA AATAAAGAGG AAAGCCTTTT GGAAAAGCGC AGGCAGCTGT CTCGTGATAT TGGTAGATTG AAAGAAACAT ATGAAGCTCT ATTAGCCAGA TTTCCCAATC TTCGATTTGC ATACAAGGAT CCAGAGAAGA ACTGGAATAG AAATTGTGTG AAAGGACTTG TGGCTTCTCT GATTAGTGTG AAAGACACTT CTGCAACCAC AGCTTTAGAA TTAGTGGCTG GAGAACGACT CTACAATGTT GTAGTAGACA CAGAAGTTAC TGGTAAAAAG CTACTAGAAA GGGGGGAACT GAAACGTCGA TACACTATAA TTCCACTCAA TAAAATTTCA GCCAGATGTA TTGCACCAGA AACTCTGAGA GTTGCTCAGA ATCTTGTTGG CCCTGACAAC GTTCATGTGG CTCTTTCCTT GGTTGAATAT AAACCAGAAC TTCAGAAAGC AATGGAGTTT GTCTTTGGAA CAACATTTGT TTGTGACAAT ATGGATAATG CCAAAAAAGT GGCCTTTGAT AAGAGGATAA TGACTAGAAC TGTAACTCTC GGAGGTGATG TGTTTGATCC TCATGGGACA TTGAGTGGAG GTGCTCGATC CCAGGCAGCT TCCATTTTAA CCAAGTTTCA AGAACTCAAA GATGTTCAGG ATGAACTGAG AATCAAAGAG AATGAGCTGC GGGCTCTAGA AGAGGAATTA GCAGGTCTTA AAAACACTGC TGAAAAGTAT CGCCAACTAA AACAGCAGTG GGAGATGAAA ACTGAAGAGG CAGATTTATT ACAAACCAAG CTCCAGCAAA GCTCATATCA CAAGCAACAA GAAGAATTAG ATGCCCTTAA AAAAACCATT GAGGAAAGTG AGGAGACTTT GAAAAACACT AAAGAAATCC AAAGAAAAGC AGAAGAAAAA TATGAAGTAT TGGAAAATAA AATGAAAAAT GCAGAAGCTG AAAGAGAGCG AGAACTGAAA GATGCTCAGA AAAAACTGGA TTGTGCCAAA ACAAAGGCAG ATGCATCTAG CAAGAAGATG AAAGAAAAAC AACAGGAAGT TGAAGCTATC ACTCTGGAAC TGGAAGAGCT CAAGAGAGAG CATACATCTT ACAAACAACA GCTTGAAGCT GTAAATGAAG CTATCAAATC CTATGAAAGT CAGATTGAAG TAATGGCAGC TGAGGTGGCT AAAAATAAGG AGTCAGTAAA TAAAGCTCAA GAAGAGGTGA CCAAGCAAAA AGAGGTGATA ACAGCCCAAG ACACTGTAAT TAAAGCTAAA TATGCAGAAG TGGCAAAACA CAAGGAGCAA AACAATGATT CTCAGCTTAA AATTAAGGAA TTAGACCACA ACATCAGCAA ACATAAACGG GAGGCTGAAG ATGGTGCTGC AAAGGTATCC AAAATGTTGA AAGATTATGA CTGGATTAAT GCAGAGAGAC ACCTCTTTGG CCAACCCAAT AGTGCCTATG ATTTCAAAAC TAACAACCCT AAAGAAGCTG GTCAGAGACT TCAGAAGTTG CAAGAAATGA AGGAGAAACT AGGAAGAAAT GTCAATATGA GAGCTATGAA TGTATTGACA GAAGCTGAAG AGCGATACAA TGACTTGATG AAGAAGAAGA GAATTGTAGA AAATGACAAA TCCAAAATTC TTACAACTAT AGAAGACCTT GACCAGAAGA AAAACCAAGC CCTAAATATT GCATGGCAAA AGGTGAACAA GGACTTTGGG TCTATTTTTT CTACTCTTTT GCCTGGTGCT AATGCTATGC TTGCACCACC AGAGGGTCAA ACTGTTTTGG ATGGTCTGGA GTTCAAGGTT GCCTTGGGAA ATACCTGGAA AGAAAACCTA ACTGAACTTA GTGGTGGTCA GAGGTCTTTA GTGGCCTTGT CATTAATACT GTCCATGCTT CTCTTCAAAC CTGCTCCAAT TTATATCCTT GATGAGGTAG ATGCAGCCTT GGATCTTTCT CATACCCAAA ACATTGGACA GATGCTGCGT ACTCATTTCA CACATTCTCA GTTCATTGTG GTGTCACTAA AAGAAGGTAT GTTCAACAAT GCAAACGTTC TTTTCAAAAC CAAGTTTGTG GATGGTGTTT CTACAGTAGC CAGATTTACT CAATGTCAAA ATGGAAAGAT TTCAAAGGAA GCAAAATCCA AGGCAAAACC ACCCAAAGGA GCACATGTGG AAGTTGGTGG TGGAGGATCA CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGT AAAGCGGCCG CTCGAGTCTA GAGGGCCCGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AAAGAACCAG CTGGGGCTCT AGGGGGTATC CCCACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTAATTCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGT
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