pCMV-Usp19(mouse)-Neo
$113.00
In stock
SKU
PL32533
NM_027804.4;8430421I07Rik; Zmynd9;
100μg liquid plasmid(Instantly):$134
| name | pCMV-Usp19(mouse)-Neo | another name | apCMV-Usp19(mouse)-Neo |
| Insert Size (bp) | 4083bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC CACTAGTCCA GTGTGGTGGA ATTCGCCACC ATGTCTGCAG GGGCCAGTGC TACAGGGCCC AGGAGGGGGC CGCCAGGACT GGAAGAGGCC ACTAGTAAGA AGAAACAGAA GGATCGAGCA AACCTGGAAA GTAAAGATGG AGATGCTAGG AGAGTGTCCC TTCCTCGAAA GGAACCAACC AAAGATGAAT TGTTGCTCGA TTGGAGGCAG AGTGCAGATG AGGTGATTGT TAAGCTGCGC GTGGGAACAG GTCCCGTACG TCTGGAGGAT GTAGATGCTG CGTTCACAGA CACGGACTGT GTGGTGAGGC TTCCAGATGG TCGGCAGTGG GGTGGTGTCT TCTTTGCTGA AATACAAAGT TCTTGCACCA AAGTGCAGGC TCGCAAGGGT GGTCTTCTAC AGCTAGTACT ACCCAAGAAG GTGCCTCTGC TCACGTGGCC CTCTCTCCTG AAACCTCTGG GAACCCAAGA GCTGGTGCCA GGTTTGCAGT GCCAGGAGAA CGGGCAAGAG CTGTCTCCCA TTGCCCTGGA GCCAGGCTCT GAGCCCCGCA GAGCTAAACA GGAAGCCCGA AACCAGAAGC GGGCCCAGGG CCGTGGTGAG GTAGGCTCGG GGGCTGGCCC TGGGACACAG GCAGGGCCCA GCGCCAAGAG GGCTGTTCAC CTCCGCAGAG GGCCAGAAGG GGAAGGGTCC ATGGATGGCC CCGGCCCCCA GGGTGATGCC CCGTCTTTCC TGTCTGACTC AGCTACCCAG GTTGAGGCTG AGGAGAAGCT CTGTGCTCCA CCAATGAACA CTCAAACAAG TCTCTTGAGC TCAGAGAAGA GTTTAGCCCT TCTGACAGTA GAGAAGACAG TGTCCCCCAG GAATGACCCA GTCGCCCCGG TTATGGTCCA GGACAGAGAC CCTGAGCCTG AGCAAGAAGA CCAAGTCAAA GAGGAGATGG CACTTGGGGC TGATCCTACA GCCTTGGTGG AGGAACCAGA GTCTATGGTG AACCTGGCAT TTGTCAAGAA CGACTCGTAT GAGAAGGGCC CGGATTCGGT GGTGGTGCAC GTGTACGTGA AGGAGATCCG CAGGGACAGC TCCCGAGTGC TCTTCCGAGA GCAGGACTTC ACACTGATCT TCCAGACCAG GGACGGAAAC TTTCTGAGGC TGCATCCGGG CTGTGGGCCC CACACCATCT TCCGATGGCA GGTGAAGCTC AGAAACTTGA TTGAACCAGA GCAGTGTACG TTCTGTTTCA CGGCCTCTCG AATCGATATC TGCCTCCGGA AGCGGCAGAG TCAGCGCTGG GGGGGACTGG AGGCCCCTGC TACACGAGGT GCAGTGGGTG GTGCAAAGGT TGCCGTGCCG ACAGGCCCAA CCCCTTTGGA TTCAACCCCT CCAGGAGGTG GCCCCCACCC CCTGACAGGC CAGGAGGAAG CCAGGGCTGT GGAGAAGGAA AAACCCAAGG CTCGATCAGA GGACTCAGGG CTGGATGGTG TGGTGGCCCG CACCCCCTTG GAGCATGTAG CCCCAAAGCC AGACCCACAC TTGGCCTCGC CCAAACCCAC GTGTATGGTG CCTCCAATGC CGCACAGTCC AGTTAGTGGG GATAGTGTGG AGGAGGACGA AGAGGAAGAG AAGAAGGTGT GCCTGCCAGG CTTCACTGGC CTTGTCAACT TAGGGAACAC CTGCTTCATG AATAGCGTCA TTCAGTCTTT GTCCAACACT CGGGAACTTC GTGACTTCTT TCACGACCGA TCCTTTGAAG CTGAGATTAA CTACAATAAC CCATTGGGGA CTGGTGGGCG CCTCGCCATT GGCTTTGCTG TGCTGCTCCG GGCCCTATGG AAGGGTACTC ACCAAGCCTT TCAGCCCTCC AAGCTAAAGG CCATTGTGGC AAGCAAGGCC AGCCAGTTCA CAGGCTATGC ACAGCATGAT GCTCAAGAGT TCATGGCTTT CTTGTTGGAT GGGCTACATG AAGACCTCAA TCGAATCCAA AACAAACCCT ACACAGAGAC TGTGGACTCG GACGGGCGGC CCGATGAGGT GGTAGCCGAG GAAGCATGGC AGCGGCACAA GATGAGAAAT GATTCATTCA TTGTGGACCT GTTTCAGGGC CAGTACAAGT CAAAGCTGGT GTGCCCTGTG TGTGCCAAGG TCTCCATCAC TTTTGACCCG TTCCTTTATC TGCCGGTACC CTTGCCACAA AAGCAAAAGG TTCTCCCCAT ATTTTATTTT GCCAGGGAGC CCCACAGCAA GCCCATCAAG TTCCTGGTGA GTGTCAGCAA GGAGAACTCC AGCGCGAGTG AAGTGTTGGA CTCCCTCTCT CAGAGTGTCC ACGTGAAGCC TGAGAACCTG CGCCTAGCCG AGGTAATTAA GAACCGTTTC CACCGTGTTT TCTTGCCCTC CCACTCACTG GACGCTGTGT CCCCCACGGA CGTGCTCCTC TGCTTTGAGC TGCTCTCCCC AGAACTGGCT AAGGAGCGGG TAGTAGTGCT GGAGGTGCAG CAGCGCCCCC AGGTACCCAG CATCCCTATC TCCAAGTGCG CAGCCTGCCA GCGGAAGCAG CAATCAGAAG AAGAAAAGCT GAAGCGCTGT ACCCGTTGCT ACCGTGTGGG CTACTGCAAC CAGTTCTGCC AGAAAACCCA TTGGCCTGAC CACAAAGGCC TCTGCCGCCC TGAGAACATT GGCTACCCCT TCCTGGTCAG TGTGCCTGCT TCACGCCTCA CTTATGCCCG TCTTGCTCAG CTACTAGAAG GTTATGCCCG GTACTCTGTG AGTGTATTCC AACCGCCCTT CCAGCCTGGC CGCATGGCTT TGGAATCGCA GAGCCCTGGC TGTACCACGT TGCTTTCAAC CAGCTCTCTG GAGGCTGGGG ACAGTGAAAG AGAACCCATT CAGCCTTCTG AGCTCCAGCT GGTGACCCCT GTGGCTGAAG GGGATACAGG GGCTCACCGA GTATGGCCGC CTGCTGATAG GGGTCCTGTG CCTAGCACCA GTGGACTCTC TTCTGAGATG CTGGCCAGTG GGCCTATCGA AGGTTGTCCC TTGCTTGCTG GTGAGAGGGT ATCTCGGCCT GAAGCTGCTG TGCCTGGGTA CCAACACTCA AGTGAATCTG TGAATACCCA CACGCCCCAG TTCTTCATCT ATAAAATTGA TGCATCAAAC CGTGAGCAGC GGCTGGAGGA CAAAGGGGAG ACACCATTGG AGCTAGGTGA TGACTGTAGC CTGGCTCTGG TGTGGCGGAA CAATGAACGC CTGCAGGAGT TTGTGTTGGT AGCCTCCAAG GAGCTGGAAT GTGCTGAAGA TCCAGGCTCT GCTGGTGAGG CTGCCCGTGC TGGCCACTTC ACCCTGGACC AGTGCCTCAA CCTCTTTACA CGGCCTGAAG TGCTGGCACC TGAGGAGGCC TGGTACTGCC CACAGTGCAA ACAGCATCGT GAGGCCTCCA AACAGCTGCT GTTGTGGCGC CTACCGAACG TGCTGATTGT GCAGCTCAAG CGCTTCTCCT TTCGTAGTTT CATTTGGCGA GACAAGATCA ATGACTTGGT GGAGTTTCCT GTTCGGAACC TGGACTTGAG CAAGTTCTGT ATCGGTCAGA AAGAGGAGCA GCTGCCTAGC TATGACCTGT ATGCTGTCAT CAACCACTAC GGAGGCATGA TCGGTGGCCA CTATACTGCT TGTGCACGGC TGCCCAATGA TCGCAGTAGC CAGCGCAGTG ACGTGGGCTG GCGCTTGTTT GATGACAGCA CGGTGACAAC AGTAGACGAA AGCCAGGTCG TGACGCGCTA TGCCTATGTT CTCTTCTACC GTCGTCGGAA CTCTCCTGTG GAGAGACCCC CCAGGGCAAG TCACTCTGAA CACCACCCAG ACCTAGGCCC TGCAGCTGAG GCTGCTGCCA GCCAGGCTTC CCGGATTTGG CAGGAGCTCG AGGCCGAGGA GGAGATGGTA CCTGAGGGGC CTGGGCCTCT GGGTCCTTGG GGGCCCCAAG ACTGGGTGGG GCCCCCGCCA CGTGGCCCTA CCACACCAGA CGAGGGTTGC CTCCGATACT TTGTCCTGGG TACCGTGGCG GCTTTGGTGG CCCTTGTGCT CAACGTATTC TATCCTCTGG TATCTCAGAG TCGCTGGAGA TGACTCGAGT CTAGAGGGCC CGTTTAAACC CGCTGATCAG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG GCTTCTGAGG CGGAAAGAAC CAGCTGGGGC TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCGAC GGATCGGGAG ATCTCCCGAT CCCCTATGGT
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