pBiT2.1-N-AFAP1(human)
$84.00
In stock
SKU
PL32512
NM_001134647.2,AFAP1/AFAP/AFAP-110/AFAP110
100μg liquid plasmid(Instantly):$105
| name | pBiT2.1-N-AFAP1(human) | another name | apBiT2.1-N-AFAP1(human) |
| Insert Size (bp) | 2445bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | TK | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | HSV-F1：GGTCCCAGGTCCACTTCGC | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGCCTAACTG GCCGGTACCT GAGTCTAAAT GAGTCTTCGG ACCTCGCGGG GGCCGCTTAA GCGGTGGTTA GGGTTTGTCT GACGCGGGGG GAGGGGGAAG GAACGAAACA CTCTCATTCG GAGGCGGCTC GGGGTTTGGT CTTGGTGGCC ACGGGCACGC AGAAGAGCGC CGCGATCCTC TTAAGCACCC CCCCGCCCTC CGTGGAGGCG GGGGTTTGGT CGGCGGGTGG TAACTGGCGG GCCGCTGACT CGGGCGGGTC GCGCGCCCCA GAGTGTGACC TTTTCGGTCT GCTCGCAGAC CCCCGGGCGG CGCCGCCGCG GCGGCGACGG GCTCGCTGGG TCCTAGGCTC CATGGGGACC GTATACGTGG ACAGGCTCTG GAGCATCCGC ACGACTGCGG TGATATTACC GGAGACCTTC TGCGGGACGA GCCGGGTCAC GCGGCTGACG CGGAGCGTCC GTTGGGCGAC AAACACCAGG ACGGGGCACA GGTACACTAT CTTGTCACCC GGAGGCGCGA GGGACTGCAG GAGCTTCAGG GAGTGGCGCA GCTGCTTCAT CCCCGTGGCC CGTTGCTCGC GTTTGCTGGC GGTGTCCCCG GAAGAAATAT ATTTGCATGT CTTTAGTTCT ATGATGACAC AAACCCCGCC CAGCGTCTTG TCATTGGCGA AGTCGAACAC GCAGATGCAG TCGGGGCGGC GCGGTCCCAG GTCCACTTCG CATATTAAGG TGACGCGTGT GGCCTCGAAC ACCGAGCGAC CCTGCAGCGA CCCGCTTAAA AGCTTGGCAA TCCGGTACTG TTGGTAAAGC CACCATGGTG ACCGGCTACC GGCTGTTCGA GGAGATTCTC GGGAGTTCCG GTGGTGGCGG GAGCGGAGGT GGAGGCTCGA GGATGGAAGA GTTAATAGTT GAACTTCGTC TCTTTCTTGA ACTCCTGGAC CATGAATATC TAACCTCAAC TGTCAGGGAG AAAAAGGCAG TGATAACCAA CATTCTGCTA AGAATACAGT CATCCAAAGG TTTTGATGTG AAGGACCATG CTCAGAAGCA GGAGACCGCT AACAGCCTGC CAGCCCCTCC TCAGATGCCC CTGCCGGAGA TCCCTCAGCC CTGGCTGCCT CCTGACAGTG GGCCTCCACC ATTGCCAACA TCCTCCCTCC CAGAAGGTTA TTATGAGGAA GCTGTGCCGC TGAGCCCCGG AAAAGCTCCG GAATACATCA CATCAAATTA TGATTCCGAT GCGATGAGCA GCTCTTATGA GTCGTATGAT GAAGAGGAGG AGGATGGGAA GGGGAAGAAA ACCCGGCACC AGTGGCCCTC CGAGGAGGCC TCCATGGACC TGGTCAAGGA CGCCAAAATC TGCGCCTTCC TGCTGCGGAA GAAGCGGTTC GGCCAGTGGA CCAAGTTGCT CTGCGTCATC AAAGACACCA AACTGCTGTG CTATAAAAGT TCCAAGGACC AGCAGCCTCA GATGGAACTG CCACTCCAAG GCTGTAACAT TACGTACATC CCGAAAGACA GCAAAAAGAA GAAGCACGAG CTGAAGATTA CTCAGCAGGG CACGGACCCG CTTGTTCTCG CCGTCCAGAG CAAGGAACAG GCCGAGCAGT GGCTGAAGGT GATCAAAGAA GCCTACAGTG GTTGTAGTGG CCCCGTGGAT TCAGAGTGTC CTCCTCCACC AAGCTCCCCG GTGCACAAGG CAGAACTGGA GAAGAAACTG TCTTCAGAGA GACCCAGCTC AGATGGGGAG GGTGTTGTGG AAAATGGAAT TACCACATGT AATGGAAAGG AGCAAGTGAA GAGGAAGAAA AGTTCCAAAT CAGAGGCCAA GGGCACTGTG TCGAAAGTCA CTGGGAAAAA AATCACCAAG ATCATCAGTC TGGGAAAGAA AAAGCCGTCC ACAGACGAGC AGACCTCCTC AGCTGAGGAA GATGTTCCCA CCTGCGGCTA TCTGAACGTG CTCTCCAACA GCCGCTGGCG AGAGCGCTGG TGCCGAGTGA AAGATAACAA GCTCATTTTC CACAAGGACA GGACCGACCT GAAGACCCAT ATTGTGTCTA TTCCGCTCCG TGGCTGCGAG GTGATCCCGG GTTTGGATTC TAAACATCCT CTGACGTTCC GGCTGCTGCG CAACGGCCAG GAGGTTGCAG TATTGGAGGC ATCTTCTTCT GAAGACATGG GCAGGTGGAT TGGGATTTTA CTCGCAGAGA CGGGATCGTC CACAGACCCG GAGGCTCTGC ACTATGACTA CATTGATGTG GAGATGTCTG CAAGTGTCAT TCAGACAGCC AAACAGACCT TCTGTTTCAT GAACAGGCGT GTTATATCTG CTAACCCATA TCTAGGGGGC ACCTCCAACG GCTATGCCCA CCCCAGCGGG ACGGCACTTC ATTATGACGA TGTCCCGTGC ATCAACGGCT CGTGGGAACC GGAAGACGGC TTTCCTGCTT CCTGCAGCAG AGGCTTGGGA GAAGAGGTGC TTTATGATAA CGCAGGCCTG TACGATAACT TGCCGCCTCC GCACATCTTT GCCCGCTACT CTCCTGCTGA CAGAAAGGCC TCTAGGCTGT CTGCTGACAA GCTGTCCTCT AACCATTACA AATACCCTGC CTCCGCTCAG TCTGTCACTA ATACCTCTTC TGTGGGGAGG GCGTCTCTCG GGCTCAACTC GCAGCTCAAG GGTAAAAAGC CCCCCGTGGC GTCTAATGGG GTCACAGGAA AAGGGAAGAC TCTGAGCAGT CAGCCAAAGA AAGCGGATCC CGCGGCTGTT GTGAAAAGGA CGGGTTCGAA TGCTGCCCAG TACAAGTATG GCAAGAACCG GGTAGAAGCA GATGCCAAGC GGCTACAGAC CAAAGAGGAG GAGCTGCTGA AGAGGAAAGA GGCCCTGCGG AATAGGCTGG CCCAGCTCCG CAAGGAAAGA AAAGACCTTC GAGCGGCTAT TGAAGTGAAC GCCGGCAGGA AGCCGCAGGC GATCCTGGAG GAGAAGCTGA AGCAGCTGGA GGAGGAGTGC CGGCAGAAGG AGGCGGAGCG TGTCAGCCTG GAGCTGGAGC TGACGGAGGT CAAGGAGAGC CTGAAGAAAG CGCTGGCGGG CGGAGTCACC CTGGGGCTGG CCATCGAGCC CAAGTCAGGG ACATCGAGTC CACAGTCTCC AGTGTTCCGG CACCGGACCC TGGAAAACTC GCCCATCTCC AGCTGTGACA CCAGTGACAC CGAGGGCCCC GTGCCGGTGA ACAGCGCGGC CGTCTTGAAG AAGAGCCAGG CTGCCCCGGG CAGCTCCCCC TGCCGAGGGC ATGTGCTGCG GAAGGCCAAG GAATGGGAAT TGAAGAACGG GACCTAGTCT AGAGTCGGGG CGGCCGGCCG CTTCGAGCAG ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAA AATCGATAAG GATCCGTCGA CCGATGCCCT TGAGAGCCTT CAACCCAGTC AGCTCCTTCC GGTGGGCGCG GGGCATGACT ATCGTCGCCG CACTTATGAC TGTCTTCTTT ATCATGCAAC TCGTAGGACA GGTGCCGGCA GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGCGGCC GCAAATGCTA AACCACTGCA GTGGTTACCA GTGCTTGATC AGTGAGGCAC CGATCTCAGC GATCTGCCTA TTTCGTTCGT CCATAGTGGC CTGACTCCCC GTCGTGTAGA TCACTACGAT TCGTGAGGGC TTACCATCAG GCCCCAGCGC AGCAATGATG CCGCGAGAGC CGCGTTCACC GGCCCCCGAT TTGTCAGCAA TGAACCAGCC AGCAGGGAGG GCCGAGCGAA GAAGTGGTCC TGCTACTTTG TCCGCCTCCA TCCAGTCTAT GAGCTGCTGT CGTGATGCTA GAGTAAGAAG TTCGCCAGTG AGTAGTTTCC GAAGAGTTGT GGCCATTGCT ACTGGCATCG TGGTATCACG CTCGTCGTTC GGTATGGCTT CGTTCAACTC TGGTTCCCAG CGGTCAAGCC GGGTCACATG ATCACCCATA TTATGAAGAA ATGCAGTCAG CTCCTTAGGG CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCGGTGTTGT CGCTCATGGT AATGGCAGCA CTACACAATT CTCTTACCGT CATGCCATCC GTAAGATGCT TTTCCGTGAC CGGCGAGTAC TCAACCAAGT CGTTTTGTGA GTAGTGTATA CGGCGACCAA GCTGCTCTTG CCCGGCGTCT ATACGGGACA ACACCGCGCC ACATAGCAGT ACTTTGAAAG TGCTCATCAT CGGGAATCGT TCTTCGGGGC GGAAAGACTC AAGGATCTTG CCGCTATTGA GATCCAGTTC GATATAGCCC ACTCTTGCAC CCAGTTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC GGGGTGTGCA AAAACAGGCA AGCAAAATGC CGCAAAGAAG GGAATGAGTG CGACACGAAA ATGTTGGATG CTCATACTCG TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTACTA GTACGTCTCT CAAGGATAAG TAAGTAATAT TAAGGTACGG GAGGTATTGG ACAGGCCGCA ATAAAATATC TTTATTTTCA TTACATCTGT GTGTTGGTTT TTTGTGTGAA TCGATAGTAC TAACATACGC TCTCCATCAA AACAAAACGA AACAAAACAA ACTAGCAAAA TAGGCTGTCC CCAGTGCAAG TGCAGGTGCC AGAACATTTC TCT
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