pCMV-NAT10(human)-3×HA-Neo
$99.00
In stock
SKU
PL32165
NM_024662.3;ALP; Kre33; NET43;
100μg liquid plasmid(Instantly):$120
| name | pCMV-NAT10(human)-3×HA-Neo | another name | apCMV-NAT10(human)-3×HA-Neo |
| Insert Size (bp) | 3075bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCATGCA TCGGAAAAAG GTGGATAACC GAATCCGGAT TCTCATTGAG AATGGAGTAG CTGAGCGGCA AAGATCTCTC TTTGTTGTAG TTGGGGATCG AGGAAAAGAT CAGGTGGTAA TACTTCATCA CATGTTATCC AAAGCAACTG TGAAGGCTCG GCCTTCAGTG CTGTGGTGTT ATAAGAAAGA GCTGGGGTTT AGCAGTCACC GGAAGAAAAG AATGCGACAG CTGCAGAAGA AAATAAAGAA TGGAACACTG AACATAAAGC AGGACGACCC CTTTGAACTC TTCATAGCAG CCACAAACAT TCGCTACTGC TACTACAACG AGACCCACAA GATCCTGGGC AATACCTTCG GCATGTGTGT GCTGCAGGAT TTTGAAGCCT TAACTCCAAA CTTGCTGGCC AGGACTGTAG AAACAGTGGA AGGTGGTGGG CTAGTGGTCA TCCTCCTACG GACCATGAAC TCACTCAAGC AATTGTACAC AGTGACTATG GATGTGCATT CCAGGTACAG AACTGAGGCC CATCAGGATG TGGTGGGAAG ATTTAATGAA AGGTTTATTC TGTCTCTGGC CTCTTGTAAG AAGTGTCTCG TCATTGATGA CCAGCTCAAC ATCCTGCCCA TCTCCTCCCA CGTTGCCACC ATGGAGGCCC TGCCTCCCCA GACTCCGGAT GAGAGTCTTG GTCCTTCTGA TCTGGAGCTG AGGGAGTTGA AGGAGAGCTT GCAGGACACC CAGCCTGTGG GTGTGTTGGT GGACTGCTGT AAGACTCTAG ACCAGGCCAA AGCTGTCTTG AAATTTATCG AGGGCATCTC TGAAAAGACC CTGAGGAGTA CTGTTGCACT CACAGCTGCT CGAGGACGGG GAAAATCTGC AGCCCTGGGA TTGGCGATTG CTGGGGCGGT GGCATTTGGG TACTCCAATA TCTTTGTTAC CTCCCCAAGC CCTGATAACC TCCATACTCT GTTTGAATTT GTATTTAAAG GATTTGATGC TCTGCAATAT CAGGAACATC TGGATTATGA GATTATCCAG TCTCTAAATC CTGAATTTAA CAAAGCAGTG ATCAGAGTGA ATGTATTTCG AGAACACAGG CAGACTATTC AGTATATACA TCCTGCAGAT GCTGTGAAGC TGGGCCAGGC TGAACTAGTT GTGATTGATG AAGCTGCCGC CATCCCCCTC CCCTTGGTGA AGAGCCTACT TGGCCCCTAC CTTGTTTTCA TGGCATCCAC CATCAATGGC TATGAGGGCA CTGGCCGGTC ACTGTCCCTC AAGCTAATTC AGCAGCTCCG TCAACAGAGC GCCCAGAGCC AGGTCAGCAC CACTGCTGAG AATAAGACCA CGACGACAGC CAGATTGGCA TCAGCGCGGA CACTGTATGA GGTTTCCCTC CAGGAGTCAA TCCGATACGC CCCTGGGGAT GCAGTGGAGA AGTGGCTGAA TGACTTGCTG TGCCTGGATT GCCTCAACAT CACTCGGATA GTCTCAGGCT GCCCCTTGCC TGAAGCTTGT GAACTGTACT ATGTTAATAG AGATACCCTC TTTTGCTACC ACAAGGCCTC TGAAGTTTTC CTCCAACGGC TTATGGCCCT CTACGTGGCT TCTCACTACA AGAACTCTCC CAATGATCTC CAGATGCTCT CCGATGCACC TGCTCACCAT CTCTTCTGCC TTCTGCCTCC CGTGCCCCCC ACCCAGAATG CCCTTCCAGA AGTGCTTGCT GTTATCCAGG TGTGCCTTGA AGGGGAGATT TCTCGCCAGT CCATCTTGAA CAGTCTGTCT CGAGGCAAGA AGGCTTCAGG GGACCTGATT CCATGGACAG TGTCAGAACA GTTCCAAGAT CCAGACTTTG GTGGTCTGTC TGGTGGAAGG GTCGTTCGCA TTGCTGTTCA CCCAGATTAT CAAGGGATGG GCTATGGCAG CCGTGCTCTG CAGCTGCTGC AGATGTACTA TGAAGGCAGG TTTCCTTGTC TGGAGGAAAA GGTCCTTGAG ACACCACAGG AAATTCACAC CGTAAGCAGC GAGGCTGTCA GCTTGTTGGA AGAGGTCATC ACTCCCCGGA AGGACCTGCC TCCTTTACTC CTCAAATTGA ATGAGAGGCC TGCCGAACGC CTGGATTACC TGGGTGTTTC CTATGGCTTG ACCCCCAGGC TCCTCAAGTT CTGGAAACGA GCTGGATTTG TTCCTGTTTA TCTGAGACAG ACCCCGAATG ACCTGACCGG AGAGCACTCG TGCATCATGC TGAAGACGCT CACTGATGAG GATGAGGCTG ACCAGGGAGG CTGGCTTGCA GCCTTCTGGA AAGATTTCCG ACGGCGGTTC CTAGCCTTGC TCTCCTACCA GTTCAGTACC TTCTCTCCTT CCCTGGCTCT GAACATCATT CAGAACAGGA ACATGGGGAA GCCAGCCCAG CCTGCCCTGA GCCGGGAGGA GCTGGAAGCA CTCTTCCTCC CCTATGACCT GAAGCGGCTG GAGATGTATT CACGGAATAT GGTGGACTAT CACCTCATCA TGGACATGAT CCCGGCCATC TCTCGCATCT ATTTCCTGAA CCAGCTGGGG GACCTGGCCC TGTCTGCGGC TCAGTCGGCT CTTCTCTTGG GGATTGGCCT GCAGCATAAG TCTGTGGACC AGCTGGAAAA GGAGATTGAG CTGCCCTCGG GCCAGTTGAT GGGACTTTTC AACCGGATCA TCCGCAAAGT TGTGAAGCTA TTTAATGAAG TTCAGGAAAA GGCCATTGAG GAGCAGATGG TGGCAGCGAA GGATGTGGTC ATGGAGCCCA CGATGAAGAC CCTCAGTGAC GACCTAGATG AAGCAGCAAA GGAATTTCAG GAGAAACACA AGAAGGAAGT AGGGAAGCTG AAGAGCATGG ACCTCTCTGA ATACATAATC CGTGGGGACG ATGAAGAGTG GAATGAAGTT TTGAACAAAG CTGGGCCGAA CGCCTCGATC ATCAGCCTGA AAAGTGACAA GAAAAGGAAG TTAGAGGCCA AACAAGAACC CAAACAGAGC AAGAAGTTGA AGAACAGAGA GACAAAGAAC AAAAAAGATA TGAAACTGAA GCGGAAGAAA CTCGAGTACC CATACGACGT ACCTGATTAT GCAACTTATC CTTATGATGT ACCTGATTAT GCTAGCTATC CTTATGATGT CCCAGACTAC GCATAGTGAG GGCCCGATAT CTCGACAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AGCTGACGTC CTTTCCATGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCCTGGA AACGGGGGAG GCTAACTGAA ACACGGAAGG AGACAATACC GGAAGGAACC CGCGCTATGA CGGCAATAAA AAGACAGAAT AAAACGCACG GGTGTTGGGT CGTTTGTTCA TAAACGCGGG GTTCGGTCCC AGGGCTGGCA CTCTGTCGAT ACCCCACCGA GACCCCATTG GGGCCAATAC GCCCGCGTTT CTTCCTTTTC CCCACCCCAC CCCCCAAGTT CGGGTGAAGG CCCAGGGCTC GCAGCCAACG TCGGGGCGGC AGGCCCTGCC ATAGCAGATC TGCGCAGCTG GGGCTCTAGG GGGTATCCCC ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGCAT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GGGGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTA ATTCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CTGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCAATGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCGACG GATCGGGAGA TCTCCCGATC CCCTATGGTC GACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATCTGC TCCCTGCTTG TGTGTTGGAG GTCGCTGAGT AGTGCGCGAG CAAAATTTAA GCTACAACAA GGCAAGGCTT GACCGACAAT TGCATGAAGA ATCTGCTTAG G
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