pBR322-HPV16-E2(20aa)-Rluc-F2A-E2
$70.00
In stock
SKU
PL32100
100μg liquid plasmid(Instantly):$91
| name | pBR322-HPV16-E2(20aa)-Rluc-F2A-E2 | another name | apBR322-HPV16-E2(20aa)-Rluc-F2A-E2 |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Virus | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC-rop | |
| Competence | Stbl3 | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | HPV16-F4 | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GAATTCTCAT GTTTGACAGC TTATCATCGA TAAGCTTTAA TGCGGTAGTT TATCACAGTT AAATTGCTAA CGCAGTCAGG CACCGTGTAT GAAATCTAAC AATGCGCTCA TCGTCATCCT CGGCACCGTC ACCCTGGATG CTGTAGGCAT AGGCTTGGTT ATGCCGGTAC TGCCGGGCCT CTTGCGGGAT ATCGTCCATT CCGACAGCAT CGCCAGTCAC TATGGCGTGC TGCTAGCGCT ATATGCGTTG ATGCAATTTC TATGCGCACC CGTTCTCGGA GCACTGTCCG ACCGCTTTGG CCGCCGCCCA GTCCTGCTCG CTTCGCTACT TGGAGCCACT ATCGACTACG CGATCATGGC GACCACACCC GTCCTGTGGA TCCATGGCTG ATCCTGCAGG TACCAATGGG GAAGAGGGTA CGGGATGTAA TGGATGGTTT TATGTAGAGG CTGTAGTGGA AAAAAAAACA GGGGATGCTA TATCAGATGA CGAGAACGAA AATGACAGTG ATACAGGTGA AGATTTGGTA GATTTTATAG TAAATGATAA TGATTATTTA ACACAGGCAG AAACAGAGAC AGCACATGCG TTGTTTACTG CACAGGAAGC AAAACAACAT AGAGATGCAG TACAGGTTCT AAAACGAAAG TATTTGGGTA GTCCACTTAG TGATATTAGT GGATGTGTAG ACAATAATAT TAGTCCTAGA TTAAAAGCTA TATGTATAGA AAAACAAAGT AGAGCTGCAA AAAGGAGATT ATTTGAAAGC GAAGACAGCG GGTATGGCAA TACTGAAGTG GAAACTCAGC AGATGTTACA GGTAGAAGGG CGCCATGAGA CTGAAACACC ATGTAGTCAG TATAGTGGTG GAAGTGGGGG TGGTTGCAGT CAGTACAGTA GTGGAAGTGG GGGAGAGGGT GTTAGTGAAA GACACACTAT ATGCCAAACA CCACTTACAA ATATTTTAAA TGTACTAAAA ACTAGTAATG CAAAGGCAGC AATGTTAGCA AAATTTAAAG AGTTATACGG GGTGAGTTTT TCAGAATTAG TAAGACCATT TAAAAGTAAT AAATCAACGT GTTGCGATTG GTGTATTGCT GCATTTGGAC TTACACCCAG TATAGCTGAC AGTATAAAAA CACTATTACA ACAATATTGT TTATATTTAC ACATTCAAAG TTTAGCATGT TCATGGGGAA TGGTTGTGTT ACTATTAGTA AGATATAAAT GTGGAAAAAA TAGAGAAACA ATTGAAAAAT TGCTGTCTAA ACTATTATGT GTGTCTCCAA TGTGTATGAT GATAGAGCCT CCAAAATTGC GTAGTACAGC AGCAGCATTA TATTGGTATA AAACAGGTAT ATCAAATATT AGTGAAGTGT ATGGAGACAC GCCAGAATGG ATACAAAGAC AAACAGTATT ACAACATAGT TTTAATGATT GTACATTTGA ATTATCACAG ATGGTACAAT GGGCCTACGA TAATGACATA GTAGACGATA GTGAAATTGC ATATAAATAT GCACAATTGG CAGACACTAA TAGTAATGCA AGTGCCTTTC TAAAAAGTAA TTCACAGGCA AAAATTGTAA AGGATTGTGC AACAATGTGT AGACATTATA AACGAGCAGA AAAAAAACAA ATGAGTATGA GTCAATGGAT AAAATATAGA TGTGATAGGG TAGATGATGG AGGTGATTGG AAGCAAATTG TTATGTTTTT AAGGTATCAA GGTGTAGAGT TTATGTCATT TTTAACTGCA TTAAAAAGAT TTTTGCAAGG CATACCTAAA AAAAATTGCA TATTACTATA TGGTGCAGCT AACACAGGTA AATCATTATT TGGTATGAGT TTAATGAAAT TTCTGCAAGG GTCTGTAATA TGTTTTGTAA ATTCTAAAAG CCATTTTTGG TTACAACCAT TAGCAGATGC CAAAATAGGT ATGTTAGATG ATGCTACAGT GCCCTGTTGG AACTACATAG ATGACAATTT AAGAAATGCA TTGGATGGAA ATTTAGTTTC TATGGATGTA AAGCATAGAC CATTGGTACA ACTAAAATGC CCTCCATTAT TAATTACATC TAACATTAAT GCTGGTACAG ATTCTAGGTG GCCTTATTTA CATAATAGAT TGGTGGTGTT TACATTTCCT AATGAGTTTC CATTTGACGA AAACGGAAAT CCAGTGTATG AGCTTAATGA TAAGAACTGG AAATCCTTTT TCTCAAGGAC GTGGTCCAGA TTAAGTTTGC ACGAGGACGA GGACAAGGAA AACGATGGAG ACTCTTTGCC AACGTTTAAA TGTGTGTCAG GACAAAATAC TAACACATTA TGAAATGACT TCGAAAGTTT ATGATCCAGA ACAAAGGAAA CGGATGATAA CTGGTCCGCA GTGGTGGGCC AGATGTAAAC AAATGAATGT TCTTGATTCA TTTATTAATT ATTATGATTC AGAAAAACAT GCAGAAAATG CTGTTATTTT TTTACATGGT AACGCGGCCT CTTCTTATTT ATGGCGACAT GTTGTGCCAC ATATTGAGCC AGTAGCGCGG TGTATTATAC CAGACCTTAT TGGTATGGGC AAATCAGGCA AATCTGGTAA TGGTTCTTAT AGGTTACTTG ATCATTACAA ATATCTTACT GCATGGTTTG AACTTCTTAA TTTACCAAAG AAGATCATTT TTGTCGGCCA TGATTGGGGT GCTTGTTTGG CATTTCATTA TAGCTATGAG CATCAAGATA AGATCAAAGC AATAGTTCAC GCTGAAAGTG TAGTAGATGT GATTGAATCA TGGGATGAAT GGCCTGATAT TGAAGAAGAT ATTGCGTTGA TCAAATCTGA AGAAGGAGAA AAAATGGTTT TGGAGAATAA CTTCTTCGTG GAAACCATGT TGCCATCAAA AATCATGAGA AAGTTAGAAC CAGAAGAATT TGCAGCATAT CTTGAACCAT TCAAAGAGAA AGGTGAAGTT CGTCGTCCAA CATTATCATG GCCTCGTGAA ATCCCGTTAG TAAAAGGTGG TAAACCTGAC GTTGTACAAA TTGTTAGGAA TTATAATGCT TATCTACGTG CAAGTGATGA TTTACCAAAA ATGTTTATTG AATCGGACCC AGGATTCTTT TCCAATGCTA TTGTTGAAGG TGCCAAGAAG TTTCCTAATA CTGAATTTGT CAAAGTAAAA GGTCTTCATT TTTCGCAAGA AGATGCACCT GATGAAATGG GAAAATATAT CAAATCGTTC GTTGAGCGAG TTCTCAAAAA TGAACAAGTG AAACAGACTT TGAATTTTGA CCTTCTCAAG TTGGCGGGAG ACGTCGAGTC CAACCCTGGG CCCAATGATA GTACAGACCT ACGTGACCAT ATAGACTATT GGAAACACAT GCGCCTAGAA TGTGCTATTT ATTACAAGGC CAGAGAAATG GGATTTAAAC ATATTAACCA CCAGGTGGTG CCAACACTGG CTGTATCAAA GAATAAAGCA TTACAAGCAA TTGAACTGCA ACTAACGTTA GAAACAATAT ATAACTCACA ATATAGTAAT GAAAAGTGGA CATTACAAGA CGTTAGCCTT GAAGTGTATT TAACTGCACC AACAGGATGT ATAAAAAAAC ATGGATATAC AGTGGAAGTG CAGTTTGATG GAGACATATG CAATACAATG CATTATACAA ACTGGACACA TATATATATT TGTGAAGAAG CATCAGTAAC TGTGGTAGAG GGTCAAGTTG ACTATTATGG TTTATATTAT GTTCATGAAG GAATACGAAC ATATTTTGTG CAGTTTAAAG ATGATGCAGA AAAATATAGT AAAAATAAAG TATGGGAAGT TCATGCGGGT GGTCAGGTAA TATTATGTCC TACATCTGTG TTTAGCAGCA ACGAAGTATC CTCTCCTGAA ATTATTAGGC AGCACTTGGC CAACCACCCC GCCGCGACCC ATACCAAAGC CGTCGCCTTG GGCACCGAAG AAACACAGAC GACTATCCAG CGACCAAGAT CAGAGCCAGA CACCGGAAAC CCCTGCCACA CCACTAAGTT GTTGCACAGA GACTCAGTGG ACAGTGCTCC AATCCTCACT GCATTTAACA GCTCACACAA AGGACGGATT AACTGTAATA GTAACACTAC ACCCATAGTA CATTTAAAAG GTGATGCTAA TACTTTAAAA TGTTTAAGAT ATAGATTTAA AAAGCATTGT ACATTGTATA CTGCAGTGTC GTCTACATGG CATTGGACAG GACATAATGT AAAACATAAA AGTGCAATTG TTACACTTAC ATATGATAGT GAATGGCAAC GTGACCAATT TTTGTCTCAA GTTAAAATAC CAAAAACTAT TACAGTGTCT ACTGGATTTA TGTCTATATG ACAAATCTTG ATACTGCATC CACAACATTA CTGGCGTGCT TTTTGCTTTG CTTTTGTGTG CTTTTGTGTG TCTGCCTATT AATACGTCCG CTGCTTTTGT CTGTGTCTAC ATACACATCA TTAATAATAT TGGTATTACT ATTGTGGATA ACAGCAGCCT CTGCGTTTAG GTGTTTTATT GTATATATTA TATTTGTTTA TATACCATTA TTTTTAATAC ATACACATGC ACGCTTTTTA ATTACATAAT GTATATGTAC ATAATGTAAT TGTTACATAT AATTGTTGTA TACCATAACT TACTATTTTT TCTTTTTTAT TTTCATATAT AATTTTTTTT TTTGTTTGTT TGTTTGTTTT TTAATAAACT GTTATTACTT AACAATGCGA CACAAACGTT CTGCAAAACG CACAAAACGT GCATCGGCTA CCCAACTTTA TAAAACATGC AAACAGGCAG GTACATGTCC ACCTGACATT ATACCTAAGG TTGAAGGCAA AACTATTGCT GATCAAATAT TACAATATGG AAGTATGGGT GTATTTTTTG GTGGGTTAGG AATTGGAACA GGGTCGGGTA CAGGCGGACG CACTGGGTAT ATTCCATTGG GAACAAGGCC TCCCACAGCT ACAGATACAC TTGCTCCTGT AAGACCCCCT TTAACAGTAG ATCCTGTGGG CCCTTCTGAT CCTTCTATAG TTTCTTTAGT GGAAGAAACT AGTTTTATTG ATGCTGGTGC ACCAACATCT GTACCTTCCA TTCCCCCAGA TGTATCAGGA TTTAGTATTA CTACTTCAAC TGATACCACA CCTGCTATAT TAGATATTAA TAATACTGTT ACTACTGTTA CTACACATAA TAATCCCACT TTCACTGACC CATCTGTATT GCAGCCTCCA ACACCTGCAG AAACTGGAGG GCATTTTACA CTTTCATCAT CCACTATTAG TACACATAAT TATGAAGAAA TTCCTATGGA TACATTTATT GTTAGCACAA ACCCTAACAC AGTAACTAGT AGCACACCCA TACCAGGGTC TCGCCCAGTG GCACGCCTAG GATTATATAG TCGCACAACA CAACAGGTTA AAGTTGTAGA CCCTGCTTTT GTAACCACTC CCACTAAACT TATTACATAT GATAATCCTG CATATGAAGG TATAGATGTG GATAATACAT TATATTTTTC TAGTAATGAT AATAGTATTA ATATAGCTCC AGATCCTGAC TTTTTGGATA TAGTTGCTTT ACATAGGCCA GCATTAACCT CTAGGCGTAC TGGCATTAGG TACAGTAGAA TTGGTAATAA ACAAACACTA CGTACTCGTA GTGGAAAATC TATAGGTGCT AAGGTACATT ATTATTATGA TTTAAGTACT ATTGATCCTG CAGAAGAAAT AGAATTACAA ACTATAACAC CTTCTACATA TACTACCACT TCACATGCAG CCTCACCTAC TTCTATTAAT AATGGATTAT ATGATATTTA TGCAGATGAC TTTATTACAG ATACTTCTAC AACCCCGGTA CCATCTGTAC CCTCTACATC TTTATCAGGT TATATTCCTG CAAATACAAC AATTCCTTTT GGTGGTGCAT ACAATATTCC TTTAGTATCA GGTCCTGATA TACCCATTAA TATAACTGAC CAAGCTCCTT CATTAATTCC TATAGTTCCA GGGTCTCCAC AATATACAAT TATTGCTGAT GCAGGTGACT TTTATTTACA TCCTAGTTAT TACATGTTAC GAAAACGACG TAAACGTTTA CCATATTTTT TTTCAGATGT CTCTTTGGCT GCCTAGTGAG GCCACTGTCT ACTTGCCTCC TGTCCCAGTA TCTAAGGTTG TAAGCACGGA TGAATATGTT GCACGCACAA ACATATATTA TCATGCAGGA ACATCCAGAC TACTTGCAGT TGGACATCCC TATTTTCCTA TTAAAAAACC TAACAATAAC AAAATATTAG TTCCTAAAGT ATCAGGATTA CAATACAGGG TATTTAGAAT ACATTTACCT GACCCCAATA AGTTTGGTTT TCCTGACACC TCATTTTATA ATCCAGATAC ACAGCGGCTG GTTTGGGCCT GTGTAGGTGT TGAGGTAGGT CGTGGTCAGC CATTAGGTGT GGGCATTAGT GGCCATCCTT TATTAAATAA ATTGGATGAC ACAGAAAATG CTAGTGCTTA TGCAGCAAAT GCAGGTGTGG ATAATAGAGA ATGTATATCT ATGGATTACA AACAAACACA ATTGTGTTTA ATTGGTTGCA AACCACCTAT AGGGGAACAC TGGGGCAAAG GATCCCCATG TACCAATGTT GCAGTAAATC CAGGTGATTG TCCACCATTA GAGTTAATAA ACACAGTTAT TCAGGATGGT GATATGGTTG ATACTGGCTT TGGTGCTATG GACTTTACTA CATTACAGGC TAACAAAAGT GAAGTTCCAC TGGATATTTG TACATCTATT TGCAAATATC CAGATTATAT TAAAATGGTG TCAGAACCAT ATGGCGACAG CTTATTTTTT TATTTACGAA GGGAACAAAT GTTTGTTAGA CATTTATTTA ATAGGGCTGG TACTGTTGGT GAAAATGTAC CAGACGATTT ATACATTAAA GGCTCTGGGT CTACTGCAAA TTTAGCCAGT TCAAATTATT TTCCTACACC TAGTGGTTCT ATGGTTACCT CTGATGCCCA AATATTCAAT AAACCTTATT GGTTACAACG AGCACAGGGC CACAATAATG GCATTTGTTG GGGTAACCAA CTATTTGTTA CTGTTGTTGA TACTACACGC AGTACAAATA TGTCATTATG TGCTGCCATA TCTACTTCAG AAACTACATA TAAAAATACT AACTTTAAGG AGTACCTACG ACATGGGGAG GAATATGATT TACAGTTTAT TTTTCAACTG TGCAAAATAA CCTTAACTGC AGACGTTATG ACATACATAC ATTCTATGAA TTCCACTATT TTGGAGGACT GGAATTTTGG TCTACAACCT CCCCCAGGAG GCACACTAGA AGATACTTAT AGGTTTGTAA CATCCCAGGC AATTGCTTGT CAAAAACATA CACCTCCAGC ACCTAAAGAA GATCCCCTTA AAAAATACAC TTTTTGGGAA GTAAATTTAA AGGAAAAGTT TTCTGCAGAC CTAGATCAGT TTCCTTTAGG ACGCAAATTT TTACTACAAG CAGGATTGAA GGCCAAACCA AAATTTACAT TAGGAAAACG AAAAGCTACA CCCACCACCT CATCTACCTC TACAACTGCT AAACGCAAAA AACGTAAGCT GTAAGTATTG TATGTATGTT GAATTAGTGT TGTTTGTTGT GTATATGTTT GTATGTGCTT GTATGTGCTT GTAAATATTA AGTTGTATGT GTGTTTGTAT GTATGGTATA ATAAACACGT GTGTATGTGT TTTTAAATGC TTGTGTAACT ATTGTGTCAT GCAACATAAA TAAACTTATT GTTTCAACAC CTACTAATTG TGTTGTGGTT ATTCATTGTA TATAAACTAT ATTTGCTACA TCCTGTTTTT GTTTTATATA TACTATATTT TGTAGCGCCA GCGGCCATTT TGTAGCTTCA ACCGAATTCG GTTGCATGCT TTTTGGCACA AAATGTGTTT TTTTAAATAG TTCTATGTCA GCAACTATGG TTTAAACTTG TACGTTTCCT GCTTGCCATG CGTGCCAAAT CCCTGTTTTC CTGACCTGCA CTGCTTGCCA ACCATTCCAT TGTTTTTTAC ACTGCACTAT GTGCAACTAC TGAATCACTA TGTACATTGT GTCATATAAA ATAAATCACT ATGCGCCAAC GCCTTACATA CCGCTGTTAG GCACATATTT TTGGCTTGTT TTAACTAACC TAATTGCATA TTTGGCATAA GGTTTAAACT TCTAAGGCCA ACTAAATGTC ACCCTAGTTC ATACATGAAC TGTGTAAAGG TTAGTCATAC ATTGTTCATT TGTAAAACTG CACATGGGTG TGTGCAAACC GTTTTGGGTT ACACATTTAC AAGCAACTTA TATAATAATA CTAAACTACA ATAATTCATG TATAAAACTA AGGGCGTAAC CGAAATCGGT TGAACCGAAA CCGGTTAGTA TAAAAGCAGA CATTTTATGC ACCAAAAGAG AACTGCAATG TTTCAGGACC CACAGGAGCG ACCCAGAAAG TTACCACAGT TATGCACAGA GCTGCAAACA ACTATACATG ATATAATATT AGAATGTGTG TACTGCAAGC AACAGTTACT GCGACGTGAG GTATATGACT TTGCTTTTCG GGATTTATGC ATAGTATATA GAGATGGGAA TCCATATGCT GTATGTGATA AATGTTTAAA GTTTTATTCT AAAATTAGTG AGTATAGACA TTATTGTTAT AGTTTGTATG GAACAACATT AGAACAGCAA TACAACAAAC CGTTGTGTGA TTTGTTAATT AGGTGTATTA ACTGTCAAAA GCCACTGTGT CCTGAAGAAA AGCAAAGACA TCTGGACAAA AAGCAAAGAT TCCATAATAT AAGGGGTCGG TGGACCGGTC GATGTATGTC TTGTTGCAGA TCATCAAGAA CACGTAGAGA AACCCAGCTG TAATCATGCA TGGAGATACA CCTACATTGC ATGAATATAT GTTAGATTTG CAACCAGAGA CAACTGATCT CTACTGTTAT GAGCAATTAA ATGACAGCTC AGAGGAGGAG GATGAAATAG ATGGTCCAGC TGGACAAGCA GAACCGGACA GAGCCCATTA CAATATTGTA ACCTTTTGTT GCAAGTGTGA CTCTACGCTT CGGTTGTGCG TACAAAGCAC ACACGTAGAC ATTCGTACTT TGGAAGACCT GTTAATGGGC ACACTAGGAA TTGTGTGCCC CATCTGTTCT CAGAAACCAT AATCTACCTC TACGCCGGAC GCATCGTGGC CGGCATCACC GGCGCCACAG GTGCGGTTGC TGGCGCCTAT ATCGCCGACA TCACCGATGG GGAAGATCGG GCTCGCCACT TCGGGCTCAT GAGCGCTTGT TTCGGCGTGG GTATGGTGGC AGGCCCCGTG GCCGGGGGAC TGTTGGGCGC CATCTCCTTG CATGCACCAT TCCTTGCGGC GGCGGTGCTC AACGGCCTCA ACCTACTACT GGGCTGCTTC CTAATGCAGG AGTCGCATAA GGGAGAGCGT CGACCGATGC CCTTGAGAGC CTTCAACCCA GTCAGCTCCT TCCGGTGGGC GCGGGGCATG ACTATCGTCG CCGCACTTAT GACTGTCTTC TTTATCATGC AACTCGTAGG ACAGGTGCCG GCAGCGCTCT GGGTCATTTT CGGCGAGGAC CGCTTTCGCT GGAGCGCGAC GATGATCGGC CTGTCGCTTG CGGTATTCGG AATCTTGCAC GCCCTCGCTC AAGCCTTCGT CACTGGTCCC GCCACCAAAC GTTTCGGCGA GAAGCAGGCC ATTATCGCCG GCATGGCGGC CGACGCGCTG GGCTACGTCT TGCTGGCGTT CGCGACGCGA GGCTGGATGG CCTTCCCCAT TATGATTCTT CTCGCTTCCG GCGGCATCGG GATGCCCGCG TTGCAGGCCA TGCTGTCCAG GCAGGTAGAT GACGACCATC AGGGACAGCT TCAAGGATCG CTCGCGGCTC TTACCAGCCT AACTTCGATC ACTGGACCGC TGATCGTCAC GGCGATTTAT GCCGCCTCGG CGAGCACATG GAACGGGTTG GCATGGATTG TAGGCGCCGC CCTATACCTT GTCTGCCTCC CCGCGTTGCG TCGCGGTGCA TGGAGCCGGG CCACCTCGAC CTGAATGGAA GCCGGCGGCA CCTCGCTAAC GGATTCACCA CTCCAAGAAT TGGAGCCAAT CAATTCTTGC GGAGAACTGT GAATGCGCAA ACCAACCCTT GGCAGAACAT ATCCATCGCG TCCGCCATCT CCAGCAGCCG CACGCGGCGC ATCTCGGGCA GCGTTGGGTC CTGGCCACGG GTGCGCATGA TCGTGCTCCT GTCGTTGAGG ACCCGGCTAG GCTGGCGGGG TTGCCTTACT GGTTAGCAGA ATGAATCACC GATACGCGAG CGAACGTGAA GCGACTGCTG CTGCAAAACG TCTGCGACCT GAGCAACAAC ATGAATGGTC TTCGGTTTCC GTGTTTCGTA AAGTCTGGAA ACGCGGAAGT CAGCGCCCTG CACCATTATG TTCCGGATCT GCATCGCAGG ATGCTGCTGG CTACCCTGTG GAACACCTAC ATCTGTATTA ACGAAGCGCT GGCATTGACC CTGAGTGATT TTTCTCTGGT CCCGCCGCAT CCATACCGCC AGTTGTTTAC CCTCACAACG TTCCAGTAAC CGGGCATGTT CATCATCAGT AACCCGTATC GTGAGCATCC TCTCTCGTTT CATCGGTATC ATTACCCCCA TGAACAGAAA TCCCCCTTAC ACGGAGGCAT CAGTGACCAA ACAGGAAAAA ACCGCCCTTA ACATGGCCCG CTTTATCAGA AGCCAGACAT TAACGCTTCT GGAGAAACTC AACGAGCTGG ACGCGGATGA ACAGGCAGAC ATCTGTGAAT CGCTTCACGA CCACGCTGAT GAGCTTTACC GCAGCTGCCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCGCAG CCATGACCCA GTCACGTAGC GATAGCGGAG TGTATACTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTGCAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAACAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG GCCCTTTCGT CTTCAA
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