pCMV-UBR5(human)-3×HA-Neo
$171.00
In stock
SKU
PL32093
NM_015902.6；DD5; EDD; EDD1; HYD
100μg liquid plasmid(Instantly):$192
| name | pCMV-UBR5(human)-3×HA-Neo | another name | apCMV-UBR5(human)-3×HA-Neo |
| Insert Size (bp) | 8397bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCATGAC GTCCATCCAT TTCGTGGTTC ACCCGCTGCC GGGCACCGAG GACCAGCTCA ATGACAGGTT ACGAGAAGTT TCTGAGAAGC TGAACAAATA TAATTTAAAC AGCCACCCCC CTTTGAATGT ATTGGAACAG GCTACTATTA AACAGTGTGT GGTGGGACCA AATCATGCTG CCTTTCTTCT TGAGGATGGT AGAGTTTGCA GGATTGGTTT TTCAGTACAG CCAGACAGAT TGGAATTGGG TAAACCTGAT AATAATGATG GGTCAAAGTT GAACAGCAAC TCGGGGGCAG GGAGGACGTC AAGGCCTGGT AGGACAAGCG ACTCTCCATG GTTTCTCTCA GGTTCTGAGA CTCTAGGCAG GCTGGCAGGC AACACCTTAG GAAGCCGCTG GAGTTCTGGA GTGGGTGGAA GTGGTGGAGG ATCCTCTGGT AGGTCATCAG CTGGAGCTCG AGATTCCCGC CGGCAGACTC GAGTTATTCG GACAGGACGG GATCGAGGGT CTGGGCTTTT GGGCAGTCAG CCCCAGCCAG TTATTCCAGC ATCTGTCATT CCAGAGGAGC TGATTTCACA GGCCCAAGTT GTTTTACAAG GCAAATCCAG AAGTGTCATT ATTCGAGAAC TTCAGAGAAC AAATCTTGAT GTGAACCTTG CTGTAAATAA TTTACTTAGC CGGGATGATG AAGATGGAGA TGATGGGGAT GATACAGCCA GCGAATCTTA TTTGCCTGGA GAGGATCTTA TGTCTCTCCT TGATGCCGAC ATTCATTCTG CCCACCCAAG TGTCATTATT GATGCAGATG CCATGTTTTC TGAAGACATT AGCTATTTTG GTTACCCTTC TTTTCGTCGT TCATCACTTT CCAGGCTAGG CTCATCTCGA GTTCTCCTTC TTCCCTTAGA GAGAGACTCT GAGCTGTTGC GTGAACGTGA ATCCGTTTTA CGTTTACGTG AACGAAGGTG GCTTGATGGA GCCTCATTTG ATAATGAAAG GGGTTCTACC AGCAAGGAAG GAGAGCCAAA CTTGGATAAG AAGAATACAC CTGTTCAAAG TCCAGTATCT CTAGGAGAAG ATTTGCAGTG GTGGCCTGAT AAGGATGGAA CAAAATTCAT CTGTATTGGG GCTCTGTATT CTGAACTTCT GGCTGTCAGC AGTAAAGGAG AACTTTATCA GTGGAAATGG AGTGAATCTG AGCCTTACAG AAATGCCCAG AATCCTTCAT TACATCATCC ACGAGCAACA TTTTTGGGGT TAACCAATGA AAAGATAGTC CTCCTGTCTG CAAATAGCAT AAGAGCAACT GTAGCTACAG AAAATAACAA GGTTGCTACA TGGGTGGATG AAACTTTAAG TTCTGTGGCT TCTAAATTAG AGCACACTGC TCAGACTTAC TCTGAACTTC AAGGAGAGCG GATAGTTTCT TTACATTGCT GTGCCCTTTA CACCTGCGCT CAGCTGGAAA ACAGTTTATA TTGGTGGGGT GTAGTTCCTT TTAGTCAAAG GAAGAAAATG TTAGAGAAAG CTAGAGCAAA AAATAAAAAG CCTAAATCCA GTGCTGGTAT TTCTTCAATG CCGAACATCA CTGTTGGTAC CCAGGTATGC TTGAGAAATA ATCCTCTTTA TCATGCTGGA GCAGTTGCAT TTTCAATTAG TGCTGGGATT CCTAAAGTTG GTGTCTTAAT GGAGTCAGTT TGGAATATGA ATGACAGCTG TAGATTTCAA CTTAGATCTC CTGAAAGCTT GAAAAACATG GAAAAAGCTA GCAAAACTAC TGAAGCTAAG CCTGAAAGTA AGCAGGAGCC AGTGAAAACA GAAATGGGTC CTCCACCATC TCCAGCATCC ACGTGTAGTG ATGCATCCTC AATTGCCAGC AGTGCATCAA TGCCATACAA ACGACGACGG TCAACCCCTG CACCAAAAGA AGAGGAAAAG GTGAATGAAG AGCAGTGGTC TCTTCGGGAA GTGGTTTTTG TGGAAGATGT CAAGAATGTT CCTGTTGGCA AGGTGCTAAA AGTAGATGGT GCCTATGTTG CTGTAAAATT TCCAGGAACC TCCAGTAATA CTAACTGTCA GAACAGCTCT GGTCCAGATG CTGACCCTTC TTCTCTCCTG CAGGATTGTA GGTTACTTAG AATTGATGAA TTGCAGGTTG TCAAAACTGG TGGAACACCG AAGGTTCCCG ACTGTTTCCA AAGGACTCCT AAAAAGCTTT GTATACCTGA AAAAACAGAA ATATTAGCAG TGAATGTAGA TTCCAAAGGT GTTCATGCTG TTCTGAAGAC TGGAAATTGG GTACGATACT GTATCTTTGA TCTTGCTACA GGAAAAGCAG AACAGGAAAA TAATTTTCCT ACAAGCAGCA TTGCTTTCCT TGGTCAGAAT GAGAGGAATG TAGCCATTTT CACTGCTGGA CAGGAATCTC CCATTATTCT TCGAGATGGA AATGGTACCA TCTACCCAAT GGCCAAAGAT TGCATGGGAG GAATAAGGGA TCCCGATTGG CTGGATCTTC CACCTATTAG TAGTCTTGGA ATGGGTGTGC ATTCTTTAAT AAATCTTCCT GCCAATTCAA CAATCAAAAA GAAAGCTGCT GTTATCATCA TGGCTGTAGA GAAACAAACC TTAATGCAAC ACATTCTGCG CTGTGACTAT GAGGCCTGTC GACAATATCT AATGAATCTT GAGCAAGCGG TTGTTTTAGA GCAGAATCTA CAGATGCTGC AGACATTCAT CAGCCACAGA TGTGATGGAA ATCGAAATAT TTTGCATGCT TGTGTATCAG TTTGCTTTCC AACCAGCAAT AAAGAAACTA AAGAAGAAGA GGAAGCGGAG CGTTCTGAAA GAAATACATT TGCAGAAAGG CTTTCTGCTG TTGAGGCCAT TGCAAATGCA ATATCAGTTG TTTCAAGTAA TGGCCCAGGT AATCGGGCTG GATCATCAAG TAGCCGAAGT TTGAGATTAC GGGAAATGAT GAGACGTTCG TTGAGAGCAG CTGGTTTGGG TAGACATGAA GCTGGAGCTT CATCCAGTGA CCACCAGGAT CCAGTTTCAC CCCCCATAGC TCCCCCTAGT TGGGTTCCTG ACCCTCCTGC GATGGATCCT GATGGTGACA TTGATTTTAT CCTGGCCCCC GCTGTGGGAT CTCTTACCAC AGCAGCAACC GGTACTGGTC AAGGACCAAG CACCTCCACT ATTCCAGGTC CTTCCACAGA GCCATCTGTA GTAGAATCCA AGGATCGAAA GGCGAATGCT CATTTTATAT TGAAATTGTT ATGTGACAGT GTGGTTCTCC AGCCCTATCT ACGAGAACTT CTTTCTGCCA AGGATGCAAG AGGGATGACC CCATTTATGT CAGCTGTAAG TGGCCGAGCT TATCCTGCTG CAATTACCAT CTTAGAAACT GCTCAGAAAA TTGCAAAAGC TGAAATATCC TCAAGTGAAA AAGAGGAAGA TGTATTCATG GGAATGGTTT GCCCATCAGG TACCAACCCT GATGACTCTC CTTTATATGT TTTATGTTGT AATGACACTT GCAGTTTTAC ATGGACTGGA GCAGAGCACA TTAACCAGGA TATTTTTGAG TGTCGAACTT GTGGCTTGCT GGAGTCACTG TGTTGTTGTA CGGAATGTGC AAGGGTTTGT CATAAAGGTC ATGATTGCAA ACTCAAACGG ACATCACCAA CAGCCTACTG TGATTGTTGG GAGAAATGTA AATGTAAAAC TCTTATTGCT GGACAGAAAT CTGCTCGTCT TGATCTACTT TATCGCCTGC TCACTGCTAC TAATCTGGTT ACTCTGCCAA ACAGCAGGGG AGAGCACCTC TTACTATTCT TAGTACAGAC AGTCGCAAGG CAGACGGTGG AGCATTGTCA ATACAGGCCA CCTCGAATCA GGGAAGATCG TAACCGAAAA ACAGCCAGTC CTGAAGATTC AGATATGCCA GATCATGATT TAGAGCCTCC AAGATTTGCC CAGCTTGCAT TGGAGCGTGT TCTACAGGAC TGGAATGCCT TGAAATCTAT GATTATGTTT GGGTCGCAGG AGAATAAAGA CCCTCTTAGT GCCAGCAGTA GAATAGGCCA TCTTTTGCCA GAAGAGCAAG TATACCTCAA TCAGCAAAGT GGCACAATTC GGCTGGACTG TTTCACTCAT TGCCTTATAG TTAAGTGTAC AGCAGATATT TTGCTTTTAG ATACTCTACT AGGTACACTA GTGAAAGAAC TCCAAAACAA ATATACACCT GGACGTAGAG AAGAAGCTAT TGCTGTGACA ATGAGGTTTC TACGTTCAGT GGCAAGAGTT TTTGTTATTC TGAGTGTGGA AATGGCTTCA TCCAAAAAGA AAAACAACTT TATTCCACAG CCAATTGGAA AATGCAAGCG TGTATTCCAA GCATTGCTAC CTTACGCTGT GGAAGAATTG TGCAACGTAG CAGAGTCACT GATTGTTCCT GTCAGAATGG GGATTGCTCG TCCAACTGCA CCATTTACCC TGGCTAGTAC TAGCATAGAT GCCATGCAGG GCAGTGAAGA ATTATTTTCA GTGGAACCAC TACCACCACG ACCATCATCT GATCAGTCTA GCAGCTCCAG TCAGTCTCAG TCATCCTACA TCATCAGGAA TCCACAGCAG AGGCGCATCA GCCAGTCACA GCCCGTTCGG GGCAGAGATG AAGAACAGGA TGATATTGTT TCAGCAGATG TGGAAGAGGT TGAGGTGGTG GAGGGTGTGG CTGGAGAAGA GGATCATCAT GATGAACAGG AAGAACACGG GGAAGAAAAT GCTGAGGCAG AGGGACAACA TGATGAGCAT GATGAAGACG GGAGTGATAT GGAGCTGGAC TTGTTAGCAG CAGCTGAAAC AGAAAGTGAT AGTGAAAGTA ACCACAGCAA CCAAGATAAT GCTAGTGGGC GCAGAAGCGT TGTCACTGCA GCAACTGCTG GTTCAGAAGC AGGAGCAAGC AGTGTTCCTG CCTTCTTTTC TGAAGATGAT TCTCAATCGA ATGACTCAAG TGATTCTGAT AGCAGTAGTA GTCAGAGTGA CGACATAGAA CAGGAGACCT TTATGCTTGA TGAGCCATTA GAAAGAACCA CAAATAGCTC CCATGCCAAT GGTGCTGCCC AAGCTCCCCG TTCAATGCAG TGGGCTGTCC GCAACACCCA GCATCAGCGA GCAGCCAGTA CAGCCCCTTC CAGTACATCT ACACCAGCAG CAAGTTCAGC GGGTTTGATT TATATTGATC CTTCAAACTT ACGCCGGAGT GGTACCATCA GTACAAGTGC TGCAGCTGCA GCAGCTGCTT TGGAAGCTAG CAACGCCAGC AGTTACCTAA CATCTGCAAG CAGTTTAGCC AGGGCTTACA GCATTGTCAT TAGACAAATC TCGGACTTGA TGGGCCTTAT TCCTAAGTAT AATCACCTAG TATACTCTCA GATTCCAGCA GCTGTGAAAT TGACTTACCA AGATGCAGTA AACTTACAGA ACTATGTAGA AGAAAAGCTT ATTCCCACTT GGAACTGGAT GGTCAGTATT ATGGATTCTA CTGAAGCTCA ATTACGTTAT GGTTCTGCAT TAGCATCTGC TGGTGATCCT GGACATCCAA ATCATCCTCT TCACGCTTCT CAGAATTCAG CGAGAAGAGA GAGGATGACT GCGCGAGAAG AAGCTAGCTT ACGAACACTT GAAGGCAGAC GACGTGCCAC CTTGCTTAGC GCCCGTCAAG GAATGATGTC TGCACGAGGA GACTTCCTAA ATTATGCTCT GTCTCTAATG CGGTCTCATA ATGATGAGCA TTCTGATGTT CTTCCAGTTT TGGATGTTTG CTCATTGAAG CATGTGGCAT ATGTTTTTCA AGCACTTATA TACTGGATTA AGGCAATGAA TCAGCAGACA ACATTGGATA CACCTCAACT AGAACGCAAA AGGACGCGAG AACTCTTGGA ACTGGGTATT GATAATGAAG ATTCAGAACA TGAAAATGAT GATGACACCA ATCAAAGTGC TACTTTGAAT GATAAGGATG ATGACTCTCT TCCTGCAGAA ACTGGCCAAA ACCATCCATT TTTCCGACGT TCAGACTCCA TGACATTCCT TGGGTGTATA CCCCCAAATC CATTTGAAGT GCCTCTGGCT GAAGCCATCC CCTTGGCTGA TCAGCCACAT CTGTTGCAGC CAAATGCTAG AAAGGAGGAT CTTTTTGGCC GTCCAAGTCA GGGTCTTTAT TCTTCATCTG CCAGTAGTGG GAAATGTTTA ATGGAGGTTA CAGTGGATAG AAACTGCCTA GAGGTTCTTC CAACAAAAAT GTCTTATGCT GCCAATCTGA AAAATGTAAT GAACATGCAA AACCGGCAAA AAAAAGAAGG GGAAGAACAG CCCGTGCTGC CAGAAGAAAC TGAGAGTTCA AAACCAGGGC CATCTGCTCA TGATCTTGCT GCACAATTAA AAAGTAGCTT ACTAGCAGAA ATAGGACTTA CTGAAAGTGA AGGGCCACCT CTCACATCTT TCAGGCCACA GTGTAGCTTT ATGGGAATGG TTATTTCCCA TGATATGCTG CTAGGACGTT GGCGCCTTTC TTTAGAACTG TTCGGCAGGG TATTCATGGA AGATGTTGGA GCAGAACCTG GATCAATCCT AACTGAATTG GGTGGTTTTG AGGTAAAAGA ATCAAAATTC CGCAGAGAAA TGGAAAAACT GAGAAACCAG CAGTCAAGAG ATTTGTCACT AGAGGTTGAT CGGGATCGAG ATCTTCTCAT TCAGCAGACT ATGAGGCAGC TTAACAATCA CTTTGGTCGA AGATGTGCTA CTACACCAAT GGCTGTACAC AGAGTAAAAG TCACATTTAA GGATGAGCCA GGAGAGGGCA GTGGTGTAGC ACGAAGTTTT TATACAGCCA TTGCACAAGC ATTTTTATCA AATGAAAAAT TGCCAAATCT AGAGTGTATC CAAAATGCCA ACAAAGGCAC CCACACAAGT TTAATGCAGA GATTAAGGAA CCGAGGAGAG AGAGACCGGG AAAGGGAGAG AGAAAGGGAA ATGAGGAGGA GTAGTGGTTT GCGAGCAGGT TCTCGGAGGG ACCGGGATAG AGACTTTAGA AGACAGCTTT CCATCGACAC TAGGCCCTTT AGACCAGCCT CTGAAGGGAA TCCTAGCGAT GATCCTGAGC CTTTGCCAGC ACATCGGCAG GCACTTGGAG AGAGGCTTTA TCCTCGTGTA CAAGCAATGC AACCAGCATT TGCAAGTAAA ATCACTGGCA TGTTGTTGGA ATTATCCCCA GCTCAGCTGC TTCTCCTTCT AGCAAGTGAG GATTCTCTGA GAGCAAGAGT GGATGAGGCC ATGGAACTCA TTATTGCACA TGGACGGGAA AATGGAGCTG ATAGTATCCT GGATCTTGGA TTAGTAGACT CCTCAGAAAA GGTACAGCAG GAAAACCGAA AGCGCCATGG CTCTAGTCGA AGTGTAGTAG ATATGGATTT AGATGATACA GATGATGGTG ATGACAATGC CCCTTTGTTT TACCAACCTG GGAAAAGAGG ATTTTATACT CCAAGGCCTG GCAAGAACAC AGAAGCAAGG TTGAATTGTT TCAGAAACAT TGGCAGGATT CTTGGACTAT GTCTGTTACA GAATGAACTA TGTCCTATCA CATTGAATAG ACATGTAATT AAAGTATTGC TTGGTAGAAA AGTCAATTGG CATGATTTTG CTTTTTTTGA TCCTGTAATG TATGAGAGTT TGCGGCAACT AATCCTCGCG TCTCAGAGTT CAGATGCTGA TGCTGTTTTC TCAGCAATGG ATTTGGCATT TGCAATTGAC CTGTGTAAAG AAGAAGGTGG AGGACAGGTT GAACTCATTC CTAATGGTGT AAATATACCA GTCACTCCAC AGAATGTATA TGAGTATGTG CGGAAATACG CAGAACACAG AATGTTGGTA GTTGCAGAAC AGCCCTTACA TGCAATGAGG AAAGGTCTAC TAGATGTGCT TCCAAAAAAT TCATTAGAAG ATTTAACGGC AGAAGATTTT AGGCTTTTGG TAAATGGCTG CGGTGAAGTC AATGTGCAAA TGCTGATCAG TTTTACCTCT TTCAATGATG AATCAGGAGA AAATGCTGAG AAGCTTCTGC AGTTCAAGCG TTGGTTCTGG TCAATAGTAG AGAAGATGAG CATGACAGAA CGACAAGATC TTGTTTACTT TTGGACATCA AGCCCATCAC TGCCAGCCAG TGAAGAAGGA TTCCAGCCTA TGCCCTCAAT CACAATAAGA CCACCAGATG ACCAACATCT TCCTACTGCA AATACTTGCA TTTCTCGACT TTACGTCCCA CTCTATTCCT CTAAACAGAT TCTCAAACAG AAATTGTTAC TCGCCATTAA GACCAAGAAT TTTGGTTTTG TGCTCGAGTA CCCATACGAC GTACCTGATT ATGCAACTTA TCCTTATGAT GTACCTGATT ATGCTAGCTA TCCTTATGAT GTCCCAGACT ACGCATAGTG AGGGCCCGAT ATCTCGACAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAGCTGACG TCCTTTCCAT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCCTG GAAACGGGGG AGGCTAACTG AAACACGGAA GGAGACAATA CCGGAAGGAA CCCGCGCTAT GACGGCAATA AAAAGACAGA ATAAAACGCA CGGGTGTTGG GTCGTTTGTT CATAAACGCG GGGTTCGGTC CCAGGGCTGG CACTCTGTCG ATACCCCACC GAGACCCCAT TGGGGCCAAT ACGCCCGCGT TTCTTCCTTT TCCCCACCCC ACCCCCCAAG TTCGGGTGAA GGCCCAGGGC TCGCAGCCAA CGTCGGGGCG GCAGGCCCTG CCATAGCAGA TCTGCGCAGC TGGGGCTCTA GGGGGTATCC CCACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGC ATCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGGGGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TAATTCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCTGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCAATG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCGA CGGATCGGGA GATCTCCCGA TCCCCTATGG TCGACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATCT GCTCCCTGCT TGTGTGTTGG AGGTCGCTGA GTAGTGCGCG AGCAAAATTT AAGCTACAAC AAGGCAAGGC TTGACCGACA ATTGCATGAA GAATCTGCTT AGG
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