NM_001284338.2;NEDD4-1; RPF1
100μg liquid plasmid(Instantly):$120
| name | pLV-Tet-miniCMV-NEDD4(human)-GFP-nanobody-mCherry-Puro | another name | apLV-Tet-miniCMV-NEDD4(human)-GFP-nanobody-mCherry-Puro |
| Insert Size (bp) | 3957bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression,Tetracycline induction | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Red |
| Promoter | Tet-miniCMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | miniCMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCAAATGGCA GTATTCATCC ACAATTTTAA AAGAAAAGGG GGGATTGGGG GGTACAGTGC AGGGGAAAGA ATAGTAGACA TAATAGCAAC AGACATACAA ACTAAAGAAT TACAAAAACA AATTACAAAA ATTCAAAATT TTCGGGTTTA TTACAGGGAC AGCAGAGAGA ATTCACGTGC TGCAGGTCCG AGGTTCTAGA CGAGTTTACT CCCTATCAGT GATAGAGAAC GATGTCGAGT TTACTCCCTA TCAGTGATAG AGAACGTATG TCGAGTTTAC TCCCTATCAG TGATAGAGAA CGTATGTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGTCGAGTTT ATCCCTATCA GTGATAGAGA ACGTATGTCG AGTTTACTCC CTATCAGTGA TAGAGAACGT ATGTCGAGGT AGGCGTGTAC GGTGGGAGGC CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGATCGCC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG ACTCACTATA GGGAGACCCA AGCTGTCAGA TCTCGAGCTC AAGCTTCGAA TTCTCTATAT ATTGCAGTGA ACTCCAAGCC ACCATGGCAC AAAGCTTACG ATTGCACTTT GCAGCCAGAA GAAGCAATAC TTACCCTTTG TCAGAAACCT CCGGAGATGA CTTGGATAGC CATGTTCACA TGTGCTTCAA AAGACCAACA CGGATTTCAA CGTCTAACGT TGTTCAAATG AAGCTGACTC CCAGACAGAC TGCACTAGCT CCGTTAATAA AGGAAAACGT TCAGTCTCAA GAAAGATCAT CTGTTCCCTC ATCTGAAAAT GTTAATAAAA AGAGCAGCTG TCTACAGATT TCACTACAGC CAACAAGGTA CAGTGGATAT CTTCAGTCTA GCAATGTCTT AGCTGATAGT GATGATGCTT CGTTTACTTG TATCTTGAAG GATGGTATTT ACAGTAGTGC TGTGGTCGAT AATGAATTGA ATGCTGTGAA TGATGGTCAC CTTGTAAGCA GTCCAGCCAT TTGTAGTGGT AGCCTTAGTA ACTTTTCAAC CAGTGATAAT GGGTCTTACA GCAGCAACGG TAGTGATTTT GGGTCATGTG CAAGTATCAC AAGTGGAGGT TCATATACTA ACAGTGTCAT CAGTGACAGT AGTAGTTATA CTTTTCCACC AAGTGATGAT ACTTTTTTGG GTGGAAACTT ACCTTCTGAC AGCACCTCCA ATAGAAGTGT GCCAAACAGG AATACTACTC CTTGTGAAAT TTTTTCAAGA AGTACAAGTA CAGATCCTTT TGTCCAGGAT GACTTGGAAC ATGGATTAGA GATTATGAAA TTGCCAGTGA GCAGGAACAC AAAAATTCCA CTAAAACGTT ACTCCTCCTT AGTCATTTTT CCTAGGAGTC CTTCAACTAC CCGACCGACT TCTCCAACAA GTCTGTGTAC TCTTCTGAGC AAAGGATCCT ATCAAACTTC ACACCAGTTT ATTATTTCTC CTAGTGAAAT TGCACATAAT GAGGATGGCA CTAGTGCTAA AGGATTTCTT TCAACAGCTG TCAATGGACT TCGGTTATCT AAAACAATTT GTACCCCCGG AGAAGTAAGA GACATACGGC CGCTTCACAG GAAGGGCTCG TTACAGAAGA AAATTGTTCT TTCGAATAAT ACTCCCAGAC AGACTGTCTG TGAAAAGTCA TCTGAAGGAT ATTCTTGTGT TTCAGTGCAT TTCACCCAAC GAAAAGCAGC TACATTAGAC TGTGAAACAA CAAATGGTGA TTGTAAACCA GAAATGTCAG AAATTAAGCT TAATTCTGAT TCAGAGTATA TTAAGCTCAT GCATAGGACA TCTGCATGTT TGCCATCCTC CCAAAATGTA GATTGTCAAA TAAATATCAA TGGAGAATTG GAAAGACCAC ATTCACAGAT GAACAAAAAC CATGGTATTT TACGAAGAAG TATTTCATTG GGAGGAGCTT ATCCAAATAT TTCCTGTCTA TCCAGCCTTA AGCACAATTG TTCTAAAGGG GGACCATCTC AGTTACTCAT AAAGTTTGCA TCTGGAAATG AAGGTAAAGT GGATAATTTA TCAAGAGACA GCAACAGAGA TTGCACAAAT GAACTGTCTA ATTCTTGCAA GACAAGAGAT GATTTCCTAG GTCAAGTGGA TGTTCCACTT TATCCATTAC CGACAGAAAA TCCAAGATTG GAGAGACCAT ATACATTTAA GGATTTTGTT CTTCATCCAA GAAGTCACAA ATCAAGAGTT AAAGGTTATC TGAGACTAAA AATGACTTAT TTACCTAAAA CCAGTGGCTC AGAAGATGAT AATGCAGAAC AGGCTGAGGA ATTAGAGCCT GGCTGGGTTG TTTTGGACCA ACCAGATGCT GCTTGCCATT TGCAGCAACA ACAAGAACCT TCTCCTCTAC CTCCAGGGTG GGAAGAGAGG CAGGATATCC TTGGAAGGAC CTATTATGTA AACCATGAAT CTAGAAGAAC ACAGTGGAAA AGACCAACCC CTCAGGACAA CCTAACAGAT GCTGAGAATG GCAACATTCA ACTGCAAGCA CAACGTGCAT TTACCACCAG GCGGCAGATA TCCGAGGAAA CAGAAAGTGT TGACAACCGA GAGTCTTCCG AGAACTGGGA AATTATAAGA GAAGATGAAG CCACCATGTA TAGCAACCAG GCCTTCCCAT CACCTCCACC GTCAAGTAAC TTGGATGTTC CAACTCATCT TGCAGAAGAA TTGAATGCCA GACTCACCAT TTTTGGAAAT TCAGCCGTGA GCCAGCCAGC ATCGAGCTCA AATCATTCCA GCAGAAGAGG CAGCTTACAA GCCTATACTT TTGAGGAACA ACCTACACTT CCTGTGCTTT TGCCTACTTC ATCTGGATTA CCACCAGGTT GGGAAGAAAA ACAAGATGAA AGAGGAAGAT CATATTATGT AGATCACAAT TCCAGAACGA CTACTTGGAC AAAGCCCACT GTACAGGCCA CAGTGGAGAC CAGTCAGCTG ACCTCAAGCC AGAGTTCTGC AGGCCCTCAA TCACAAGCCT CCACCAGTGA TTCAGGCCAG CAGGTGACCC AGCCATCTGA AATTGAGCAA GGATTCCTTC CTAAAGGCTG GGAAGTCCGG CATGCACCAA ATGGGAGGCC TTTCTTTATT GACCACAACA CTAAAACCAC CACCTGGGAA GATCCAAGAT TGAAAATTCC AGCCCATCTG AGAGGAAAGA CATCACTTGA TACTTCCAAT GATCTAGGGC CTTTACCTCC AGGATGGGAA GAGAGAACTC ACACAGATGG AAGAATCTTC TACATAAATC ACAATATAAA AAGAACACAA TGGGAAGATC CTCGGTTGGA GAATGTAGCA ATAACTGGAC CAGCAGTGCC CTACTCCAGG GATTACAAAA GAAAGTATGA GTTCTTCCGA AGAAAGTTGA AGAAGCAGAA TGACATTCCA AACAAATTTG AAATGAAACT TCGCCGAGCA ACTGTTCTTG AAGACTCTTA CCGGAGAATT ATGGGTGTCA AGAGAGCAGA CTTCCTGAAG GCTCGACTGT GGATTGAGTT TGATGGTGAA AAGGGATTGG ATTATGGAGG AGTTGCCAGA GAATGGTTCT TCCTGATCTC AAAGGAAATG TTTAACCCTT ATTATGGGTT GTTTGAATAT TCTGCTACGG ACAATTATAC CCTACAGATA AATCCAAACT CTGGATTGTG TAACGAAGAT CACCTCTCTT ACTTCAAGTT TATTGGTCGG GTAGCTGGAA TGGCAGTTTA TCATGGCAAA CTGTTGGATG GTTTTTTCAT CCGCCCATTT TACAAGATGA TGCTTCACAA ACCAATAACC CTTCATGATA TGGAATCTGT GGATAGTGAA TATTACAATT CCCTAAGATG GATTCTTGAA AATGACCCAA CAGAATTGGA CCTCAGGTTT ATCATAGATG AAGAACTTTT TGGACAGACA CATCAACATG AGCTGAAAAA TGGTGGATCA GAAATAGTTG TCACCAATAA GAACAAAAAG GAATATATTT ATCTTGTAAT ACAATGGCGA TTTGTAAACC GAATCCAGAA GCAAATGGCT GCTTTTAAAG AGGGATTCTT TGAACTAATA CCACAGGATC TCATCAAAAT TTTTGATGAA AATGAACTAG AGCTTCTTAT GTGTGGACTG GGAGATGTTG ATGTGAATGA CTGGAGGGAA CATACAAAGT ATAAAAATGG CTACAGTGCA AATCATCAGG TTATACAGTG GTTTTGGAAG GCTGTTTTAA TGATGGATTC AGAAAAAAGA ATAAGATTAC TTCAGTTTGT CACTGGCACA TCTCGGGTGC CTATGAATGG ATTTGCTGAA CTATACGGTT CAAATGGACC ACAGTCATTT ACAGTTGAAC AGTGGGGTAC TCCTGAAAAG CTGCCAAGAG CTCATACCTG TTTTAATCGC CTGGACTTGC CACCTTATGA ATCATTTGAA GAATTATGGG ATAAACTTCA GATGGCAATT GAAAACACCC AGGGCTTTGA TGGAGTTGAT TACCCCTACG ACGTGCCTGA CTACGCCGGC GGCGGTGGTT CAGGCGGTGG CGGTTCCGGT GGTGGGGGAT CAGTCCAACT GGTGGAGTCT GGTGGCGCTT TGGTGCAGCC AGGTGGCTCT CTGCGTTTGT CCTGTGCCGC TTCTGGCTTC CCAGTGAACC GCTATTCCAT GCGCTGGTAT CGCCAGGCTC CAGGCAAAGA GCGTGAGTGG GTAGCCGGTA TGTCCAGCGC GGGTGATCGT AGCTCCTATG AAGACTCCGT GAAGGGCCGT TTCACCATCA GCCGTGACGA TGCCCGTAAC ACGGTGTATC TGCAAATGAA CAGCTTGAAA CCTGAAGATA CGGCCGTGTA TTACTGTAAT GTGAACGTGG GCTTCGAGTA TTGGGGCCAA GGCACCCAGG TCACCGTCTC CAGCTAAGAT CCTTGACTTG CGGCCGCAAC TCCCACCTGC AACATGCGTG ACTGACTGAG GCCGCGACTC TAGAGTCGAC CTGCATCTAG GCGCCGGAAT TAGATCTCTC GAGGTTAACG AATTCTACCG GGTAGGGGAG GCGCTTTTCC CAAGGCAGTC TGGAGCATGC GCTTTAGCAG CCCCGCTGGG CACTTGGCGC TACACAAGTG GCCTCTGGCC TCGCACACAT TCCACATCCA CCGGTAGGCG CCAACCGGCT CCGTTCTTTG GTGGCCCCTT CGCGCCACCT TCTACTCCTC CCCTAGTCAG GAAGTTCCCC CCCGCCCCGC AGCTCGCGTC GTGCAGGACG TGACAAATGG AAGTAGCACG TCTCACTAGT CTCGTGCAGA TGGACAGCAC CGCTGAGCAA TGGAAGCGGG TAGGCCTTTG GGGCAGCGGC CAATAGCAGC TTTGCTCCTT CGCTTTCTGG GCTCAGAGGC TGGGAAGGGG TGGGTCCGGG GGCGGGCTCA GGGGCGGGCT CAGGGGCGGG GCGGGCGCCC GAAGGTCCTC CGGAGGCCCG GCATTCTGCA CGCTTCAAAA GCGCACGTCT GCCGCGCTGT TCTCCTCTTC CTCATCTCCG GGCCTTTCGA CCTGCAGCCC AAGCTTACCA TGGTGAGCAA GGGCGAGGAG GATAACATGG CCATCATCAA GGAGTTCATG CGCTTCAAGG TGCACATGGA GGGCTCCGTG AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC GCCCCTACGA GGGCACCCAG ACCGCCAAGC TGAAGGTGAC CAAGGGTGGC CCCCTGCCCT TCGCCTGGGA CATCCTGTCC CCTCAGTTCA TGTACGGCTC CAAGGCCTAC GTGAAGCACC CCGCCGACAT CCCCGACTAC TTGAAGCTGT CCTTCCCCGA GGGCTTCAAG TGGGAGCGCG TGATGAACTT CGAGGACGGC GGCGTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACG GCGAGTTCAT CTACAAGGTG AAGCTGCGCG GCACCAACTT CCCCTCCGAC GGCCCCGTAA TGCAGAAGAA GACCATGGGC TGGGAGGCCT CCTCCGAGCG GATGTACCCC GAGGACGGCG CCCTGAAGGG CGAGATCAAG CAGAGGCTGA AGCTGAAGGA CGGCGGCCAC TACGACGCTG AGGTCAAGAC CACCTACAAG GCCAAGAAGC CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT GGACATCACC TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG CCGCCACTCC ACCGGCGGCA TGGACGAGCT GTACAAGGCA ACAAACTTCT CTCTGCTGAA ACAAGCCGGA GATGTCGAAG AGAATCCTGG ACCGATGACC GAGTACAAGC CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCAGGGC CGTACGCACC CTCGCCGCCG CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGATCC GGACCGCCAC ATCGAGCGGG TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGGTGCCCG AAGGACCGCG CACCTGGTGC ATGACCCGCA AGCCCGGTGC CTGATCTTAT CGATAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAA TCATCGTCCT TTCCTTGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCATCGATA CCGTCGACCA AGGCAGCTGT AGATCTTAGC CACTTTTTAA AAGAAAAGGG GGGACTGGAA GGGCTAATTC ACTCCCAACG AAGACAAGAT CTGCTTTTTG CTTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG CATCTAGAAT TAATTCCGTG TATTCTATAG TGTCACCTAA ATCGTATGTG TATGATACAT AAGGTTATGT ATTAATTGTA GCCGCGTTCT AACGACAATA TGTACAAGCC TAATTGTGTA GCATCTGGCT TACTGAAGCA GACCCTATCA TCTCTCTCGT AAACTGCCGT CAGAGTCGGT TTGGTTGGAC GAACCTTCTG AGTTTCTGGT AACGCCGTCC CGCACCCGGA AATGGTCAGC GAACCAATCA GCAGGGTCAT CGCTAGCCAG ATCCTCTACG CCGGACGCAT CGTGGCCGGC ATCACCGGCG CCACAGGTGC GGTTGCTGGC GCCTATATCG CCGACATCAC CGATGGGGAA GATCGGGCTC GCCACTTCGG GCTCATGAGC GCTTGTTTCG GCGTGGGTAT GGTGGCAGGC CCCGTGGCCG GGGGACTGTT GGGCGCCATC TCCTTGCATG CACCATTCCT TGCGGCGGCG GTGCTCAACG GCCTCAACCT ACTACTGGGC TGCTTCCTAA TGCAGGAGTC GCATAAGGGA GAGCGTCGAA TGGTGCACTC TCAGTACAAT CTAGCTCTGA TGCCGCATAG TTAAGCCAGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTTGGAC ACAAGACAGG CTTGCGAGAT ATGTTTGAGA ATACCACTTT ATCCCGCGTC AGGGAGAGGC AGTGCGTAAA AAGACGCGGA CTCATGTGAA ATACTGGTTT TTAGTGCGCC AGATCTCTAT AATCTCGCGC AACCTATTTT CCCCTCGAAC ACTTTTTAAG CCGTAGATAA ACAGGCTGGG ACACTTCACA TGAGCGAAAA ATACATCGTC ACCTGGGACA TGTTGCAGAT CCATGCACGT AAACTCGCAA GCCGACTGAT GCCTTCTGAA CAATGGAAAG GCATTATTGC CGTAAGCCGT GGCGGTCTGT ACCGGGTGCG TTACTGGCGC GTGAACTGGG TATTCGTCAT GTCGATACCG TTTGTATTTC CAGCTACGAT CACGACAACC AGCGCGAGCT TAAAGTGCTG AAACGCGCAG AAGGCGATGG CGAAGGCTTC ATCGTTATTG ATGACCTGGT GGATACCGGT GGTACTGCGG TTGCGATTCG TGAAATGTAT CCAAAAGCGC ACTTTGTCAC CATCTTCGCA AAACCGGCTG GTCGTCCGCT GGTTGATGAC TATGTTGTTG ATATCCCGCA AGATACCTGG ATTGAACAGC CGTGGGATAT GGGCGTCGTA TTCGTCCCGC CAATCTCCGG TCGCTAATCT TTTCAACGCC TGGCACTGCC GGGCGTTGTT CTTTTTAACT TCAGGCGGGT TACAATAGTT TCCAGTAAGT ATTCTGGAGG CTGCATCCAT GACACAGGCA AACCTGAGCG AAACCCTGTT CAAACCCCGC TTTAAACATC CTGAAACCTC GACGCTAGTC CGCCGCTTTA ATCACGGCGC ACAACCGCCT GTGCAGTCGG CCCTTGATGG TAAAACCATC CCTCACTGGT ATCGCATGAT TAACCGTCTG ATGTGGATCT GGCGCGGCAT TGACCCACGC GAAATCCTCG ACGTCCAGGC ACGTATTGTG ATGAGCGATG CCGAACGTAC CGACGATGAT TTATACGATA CGGTGATTGG CTACCGTGGC GGCAACTGGA TTTATGAGTG GGCCCCGGAT CTTTGTGAAG GAACCTTACT TCTGTGGTGT GACATAATTG GACAAACTAC CTACAGAGAT TTAAAGCTCT AAGGTAAATA TAAAATTTTT AAGTGTATAA TGTGTTAAAC TACTGATTCT AATTGTTTGT GTATTTTAGA TTCCAACCTA TGGAACTGAT GAATGGGAGC AGTGGTGGAA TGCCTTTAAT GAGGAAAACC TGTTTTGCTC AGAAGAAATG CCATCTAGTG ATGATGAGGC TACTGCTGAC TCTCAACATT CTACTCCTCC AAAAAAGAAG AGAAAGGTAG AAGACCCCAA GGACTTTCCT TCAGAATTGC TAAGTTTTTT GAGTCATGCT GTGTTTAGTA ATAGAACTCT TGCTTGCTTT GCTATTTACA CCACAAAGGA AAAAGCTGCA CTGCTATACA AGAAAATTAT GGAAAAATAT TCTGTAACCT TTATAAGTAG GCATAACAGT TATAATCATA ACATACTGTT TTTTCTTACT CCACACAGGC ATAGAGTGTC TGCTATTAAT AACTATGCTC AAAAATTGTG TACCTTTAGC TTTTTAATTT GTAAAGGGGT TAATAAGGAA TATTTGATGT ATAGTGCCTT GACTAGAGAT CATAATCAGC CATACCACAT TTGTAGAGGT TTTACTTGCT TTAAAAAACC TCCCACACCT CCCCCTGAAC CTGAAACATA AAATGAATGC AATTGTTGTT GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGATCAA CTGGATAACT CAAGCTAACC AAAATCATCC CAAACTTCCC ACCCCATACC CTATTACCAC TGCCAATTAC CTAGTGGTTT CATTTACTCT AAACCTGTGA TTCCTCTGAA TTATTTTCAT TTTAAAGAAA TTGTATTTGT TAAATATGTA CTACAAACTT AGTAGTTGGA AGGGCTAATT CACTCCCAAA GAAGACAAGA TATCCTTGAT CTGTGGATCT ACCACACACA AGGCTACTTC CCTGATTAGC AGAACTACAC ACCAGGGCCA GGGGTCAGAT ATCCACTGAC CTTTGGATGG TGCTACAAGC TAGTACCAGT TGAGCCAGAT AAGGTAGAAG AGGCCAATAA AGGAGAGAAC ACCAGCTTGT TACACCCTGT GAGCCTGCAT GGGATGGATG ACCCGGAGAG AGAAGTGTTA GAGTGGAGGT TTGACAGCCG CCTAGCATTT CATCACGTGG CCCGAGAGCT GCATCCGGAG TACTTCAAGA ACTGCTGATA TCGAGCTTGC TACAAGGGAC TTTCCGCTGG GGACTTTCCA GGGAGGCGTG GCCTGGGCGG GACTGGGGAG TGGCGAGCCC TCAGATCCTG CATATAAGCA GCTGCTTTTT GCCTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GTGGCGCCCG AACAGGGACT TGAAAGCGAA AGGGAAACCA GAGGAGCTCT CTCGACGCAG GACTCGGCTT GCTGAAGCGC GCACGGCAAG AGGCGAGGGG CGGCGACTGG TGAGTACGCC AAAAATTTTG ACTAGCGGAG GCTAGAAGGA GAGAGATGGG TGCGAGAGCG TCAGTATTAA GCGGGGGAGA ATTAGATCGC GATGGGAAAA AATTCGGTTA AGGCCAGGGG GAAAGAAAAA ATATAAATTA AAACATATAG TATGGGCAAG CAGGGAGCTA GAACGATTCG CAGTTAATCC TGGCCTGTTA GAAACATCAG AAGGCTGTAG ACAAATACTG GGACAGCTAC AACCATCCCT TCAGACAGGA TCAGAAGAAC TTAGATCATT ATATAATACA GTAGCAACCC TCTATTGTGT GCATCAAAGG ATAGAGATAA AAGACACCAA GGAAGCTTTA GACAAGATAG AGGAAGAGCA AAACAAAAGT AAGACCACCG CACAGCAAGC GGCCGGTGAT CTTCAGACCT GGACGATATA TATGAGGGAC AATTGGAGAA GTGAATTATA TAAATATAAA GTAGTAAAAA TTGAACCATT AGGAGTAGCA CCCACCAAGG CAAAGAGAAG AGTGGTGCAG AGAGAAAAAA GAGCAGTGGG AATAGGAGCT TTGTTCCTTG GGTTCTTGGG AGCAGCAGGA AGCACTATGG GCGCAGCGTC AATGACGCTG ACGGTACAGG CCAGACAATT ATTGTCTGGT ATAGTGCAGC AGCAGAACAA TTTGCTGAGG GCTATTGAGG CGCAACAGCA TCTGTTGCAA CTCACAGTCT GGGGCATCAA GCAGCTCCAG GCAAGAATCC TGGCTGTGGA AAGATACCTA AAGGATCAAC AGCTCCTGGG GATTTGGGGT TGCTCTGGAA AACTCATTTG CACCACTGCT GTGCCTTGGA ATGCTAGTTG GAGTAATAAA TCTCTGGAAC AGATTTGGAA TCACACGACC TGGATGGAGT GGGACAGAGA AATTAACAAT TACACAAGCT TAATACACTC CTTAATTGAA GAATCGCAAA ACCAGCAAGA AAAGAATGAA CAAGAATTAT TGGAATTAGA TAAATGGGCA AGTTTGTGGA ATTGGTTTAA CATAACAAAT TGGCTGTGGT ATATAAAATT ATTCATAATG ATAGTAGGAG GCTTGGTAGG TTTAAGAATA GTTTTTGCTG TACTTTCTAT AGTGAATAGA GTTAGGCAGG GATATTCACC ATTATCGTTT CAGACCCACC TCCCAACCCC GAGGGGACCC GACAGGCCCG AAGGAATAGA AGAAGAAGGT GGAGAGAGAG ACAGAGACAG ATCCATTCGA TTAGTGAACG GATCTCGACG GTCG
