pSLC1A1(human)-Fluc
$70.00
In stock
SKU
PL31856
-
100μg liquid plasmid(Instantly):$91
| name | pSLC1A1(human)-Fluc | another name | apSLC1A1(human)-Fluc |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Luciferase Report | Fragment type | Promoter |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Fluc | |
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGTACCTGCC CAAGGTCACC AAGCTGATAA GTGGTGGTGC TGGGATTTAA GCCTAAATAC TTTACCCCGA GAACCCTAGT GTAATTGTCT TATTACAACC CAGAAAGTTG TGATGTACAC ATAGTTCCTC AGATGCTTCC ACTTTTTTCT CCCCTCAGGA ATGTTTATTT TGTAAATACT GCCCTATCTA CAACTGTAAT GAGCATGGGT ATGCTTATGG TCAACACTTT TTTTTTTTTT TAAATAGATA GAGTCTCGCT CTGTCACCCA GGCTGGAGTA CAGTGGTACA ATTATAGCTC CCTGCAACCT CGAACTCCCG GGCTCAAACT ATCCTCTTGC CTCAGCCTCC CAAGTAGCAG AGACTACAGG CATGTGCCAT CATGGCCAGA TTATTATTAT TATTAAGTTT TGTAGAATCA AGATTTCACT AAGTTGCCCA GGTTGGTCTC AAACTCCTAG CCTCAAGTGA TCCTTCTGCC TCAGCCTCCC AAACTGCTGG GAATATAATT GTGAGCCAAC ACGCCCAGCT CTATGGTAGA CACTTTGGAA ATATAAAAGA TAGAAGGCAA AGATGACCAT TGTTAAAATT TTGGCATATT TCTTCTGGTC TTTTTTTCTA AGCACATGGT TTTATTTCGT CTGTTTGTTG GTTGGTTGGT CGGTTTAGTT TTTGTAGAGA CAGTGGTCTA ATTATGTTGT CCAGGCTGGT CCTAAACTCC TGGGCTTAAG CAGTCCTACC ATCTCGGCTT CCCAAAGTGC TGGGATTACG GGTGTGAGCC ACTGCACCTG GCCAATATGT TTATTTTACA CACATGTGAT CAAATTGTAC ATGCAATTTT AGTATTTCTT TCTTCCCTTT ATATTTATAA ATATAGATTT ATAACATGTG CCATCCCATG CTGTTAAAAA CTTCATAAAC TTCCTTTATG TAGGCTGCAT AAGGTACTGT TGCTTGGCAG TTCCATAATA TATTTACCAT TCCATTTATG TTGGACATTT GCGTTTTTTC CAGCTGTTTT TTTACTGTGG GACAAATCTG TTCTCTCGCG CACGCTCTCT CTCTTTTTTT TTTATGAGAC AGGGTTTCCC TCCGGTCGCC CAGGCAGGAG TGCTGCAGCG TGATCTCGGC TCACCGCAAC CTCCACCTCC TGGGTTCAAG TGATTCTCCT GTCTCAGCCT CCCGAATAGC TGGGATTACA GGTGCATGGC CCTAAACCCG GCTAATTTTT GTATTTTTGG TAGACGGGAT TTCCCCATGT TGGCCAGGCT GGTCTCGAAC TCTTGACCTC AAGTGATCCG CCTGCCTTGG CCTCCCAAAT TGCTGGAATT ACAGGTGTGA GCCATCGCGC CCGGGACTAT TCTCCCTTTC TTGATAGTTT CCCTTCTGAC ATTTCCTTCC TTCTTTCTTT CTTTTTTCTT TTTTTTTGAC AGTCTCGCTC TGTCGCCTAG GCTGGAGCGC AGTGGCTTGA TCTCAGCTTA CTGCAATCTC CACCTTCCAG GTTCAAGCGA TTCTCCTCCC TCAGCCTCCC AAGTAGCTGC GACTACAGGC ACGTGCCACC ACGCCCGGCT AATTTTTTTG TATTTTTAGC AGAGACGGGG TTTCACTGTG TTAGCCAGGA TGGTCTCAAT CTCCTGACCT CGTGATCCGC TCGCCTCGGA CTCCCAAAGT CCTGAGATTA CAGGCAAGAG CCACCGCACC CGGGTCCCCT TCTGACATTT CTTCAGATCT TCCTCGGACA CCCAACGACC TTAAAATCCC TCTAACCTTC TTTCTCTCAA AGTTTACTTT CTATAAAGAG CTGATAAACT CATTCATACT AAAGTCTACA GGGTTGTAAA TCTTAATCTA TTATCGCTTA AAAGATGCTT GTATCAATTT ACACCAAAGG GATGAAGCTC TAATTGTGGG GATTTAGTCA TCAGAAAATC TAGTAGGGGA AATTCTCCCT ACCCACAATC AGAGTAGATT CTACAATAGC AAAAAGGGTT CCAGGCTCAC TTAACTTTAG GAACGCTGAT AAGAAGACCG CTTACAGCTG CGGAGGTACT GATTTGGTAA AGGGAGGGAA AGAGATTGTA TACGTGAGAG TGAAAAAATT GGGTAGAAAA ATGGAAGAGC ATCAGAAATT CACTTAGCAA CACCAAATAT ATTTGGCACC ACTAACCTAG TTTCTCCTGT GTTTGCCTTT CCAATCCGTT GGACCTTAGG GGATGGACCG CCCACTTTCT CCCCAGAGCG GCTCCCGGGT CCGGTTCTCA GTTCTAATCA CTGCCCCAAG CCTCCATGCT CCAGGGCCTG TGGTCACAGA GCAAGTCCCA AGGAGCAGAA GTAAAGTTCT AAGAGAAATC ACCATCTCCT TGTGCAGGGA ACACTCCCAA GCCCAGTGTC CACAGACAAA TCACCTCCAG CGACTCGAAG AGGAGAATAA TCCCTGTGGG GGCACTGGAG GCTCCGCGTG AAAGTTTCCA AAGCCATGTT CTGCCCCAGG ACTCCTTTCT CTCCTCCCAG AAGCTTCAGG CTTTCGCGGC TGCTGGCACA TCCCAGCCTG AGTAGCCGAG CAAGAGGGAC CCAAGGCAGG ATCAGCTTCC CCCGACTTGA AAGGGCACCC GTTCTGCAGG TGCCGGCGCT GGAAATAGCT GGTGTGGAGA ACGGAGAGGG AGACGTGCAG GATGAAGTCC GCGCCCAGGA GGGCAGTGGC TCCGCCTCTC TGGGTTAGGG ACAGAAGAAA ACCCTTAACC CAAAGCCCTA GCATGAGTGA GGCGTGGAAA GCGAGACAGA CTCCAGTGGA AGGCTGTGGG GCGGGACTCA GGGTTCCCGG AGAGGCCGCC CCAGCTCCTG CCGCAGCGCT GGGCGCCCTA CCACGCCTGC TCCCTAGGGA CCCCGGGCGT CCCACGCACG CACCCACCCT CCTTCCCGGG CCTGCCGCTC CTCCCTCCTT CCTCGCTGGT TGGCCCAGGC GCCGCAGAGC GCGGCGGCAG CAGGAGGAGC CGGGCGCGCC TGCCACGCTC GAGATCTGCG ATCTAAGTAA GCTTGGCATT CCGGTACTGT TGGTAAAGCC ACCATGGAAG ACGCCAAAAA CATAAAGAAA GGCCCGGCGC CATTCTATCC GCTGGAAGAT GGAACCGCTG GAGAGCAACT GCATAAGGCT ATGAAGAGAT ACGCCCTGGT TCCTGGAACA ATTGCTTTTA CAGATGCACA TATCGAGGTG GACATCACTT ACGCTGAGTA CTTCGAAATG TCCGTTCGGT TGGCAGAAGC TATGAAACGA TATGGGCTGA ATACAAATCA CAGAATCGTC GTATGCAGTG AAAACTCTCT TCAATTCTTT ATGCCGGTGT TGGGCGCGTT ATTTATCGGA GTTGCAGTTG CGCCCGCGAA CGACATTTAT AATGAACGTG AATTGCTCAA CAGTATGGGC ATTTCGCAGC CTACCGTGGT GTTCGTTTCC AAAAAGGGGT TGCAAAAAAT TTTGAACGTG CAAAAAAAGC TCCCAATCAT CCAAAAAATT ATTATCATGG ATTCTAAAAC GGATTACCAG GGATTTCAGT CGATGTACAC GTTCGTCACA TCTCATCTAC CTCCCGGTTT TAATGAATAC GATTTTGTGC CAGAGTCCTT CGATAGGGAC AAGACAATTG CACTGATCAT GAACTCCTCT GGATCTACTG GTCTGCCTAA AGGTGTCGCT CTGCCTCATA GAACTGCCTG CGTGAGATTC TCGCATGCCA GAGATCCTAT TTTTGGCAAT CAAATCATTC CGGATACTGC GATTTTAAGT GTTGTTCCAT TCCATCACGG TTTTGGAATG TTTACTACAC TCGGATATTT GATATGTGGA TTTCGAGTCG TCTTAATGTA TAGATTTGAA GAAGAGCTGT TTCTGAGGAG CCTTCAGGAT TACAAGATTC AAAGTGCGCT GCTGGTGCCA ACCCTATTCT CCTTCTTCGC CAAAAGCACT CTGATTGACA AATACGATTT ATCTAATTTA CACGAAATTG CTTCTGGTGG CGCTCCCCTC TCTAAGGAAG TCGGGGAAGC GGTTGCCAAG AGGTTCCATC TGCCAGGTAT CAGGCAAGGA TATGGGCTCA CTGAGACTAC ATCAGCTATT CTGATTACAC CCGAGGGGGA TGATAAACCG GGCGCGGTCG GTAAAGTTGT TCCATTTTTT GAAGCGAAGG TTGTGGATCT GGATACCGGG AAAACGCTGG GCGTTAATCA AAGAGGCGAA CTGTGTGTGA GAGGTCCTAT GATTATGTCC GGTTATGTAA ACAATCCGGA AGCGACCAAC GCCTTGATTG ACAAGGATGG ATGGCTACAT TCTGGAGACA TAGCTTACTG GGACGAAGAC GAACACTTCT TCATCGTTGA CCGCCTGAAG TCTCTGATTA AGTACAAAGG CTATCAGGTG GCTCCCGCTG AATTGGAATC CATCTTGCTC CAACACCCCA ACATCTTCGA CGCAGGTGTC GCAGGTCTTC CCGACGATGA CGCCGGTGAA CTTCCCGCCG CCGTTGTTGT TTTGGAGCAC GGAAAGACGA TGACGGAAAA AGAGATCGTG GATTACGTCG CCAGTCAAGT AACAACCGCG AAAAAGTTGC GCGGAGGAGT TGTGTTTGTG GACGAAGTAC CGAAAGGTCT TACCGGAAAA CTCGACGCAA GAAAAATCAG AGAGATCCTC ATAAAGGCCA AGAAGGGCGG AAAGATCGCC GTGTAATTCT AGAGTCGGGG CGGCCGGCCG CTTCGAGCAG ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAA AATCGATAAG GATCCGTCGA CCGATGCCCT TGAGAGCCTT CAACCCAGTC AGCTCCTTCC GGTGGGCGCG GGGCATGACT ATCGTCGCCG CACTTATGAC TGTCTTCTTT ATCATGCAAC TCGTAGGACA GGTGCCGGCA GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC AATGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA ACGTTTACAA TTTCCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCCCAAG CTACCATGAT AAGTAAGTAA TATTAAGGTA CGGGAGGTAC TTGGAGCGGC CGCAATAAAA TATCTTTATT TTCATTACAT CTGTGTGTTG GTTTTTTGTG TGAATCGATA GTACTAACAT ACGCTCTCCA TCAAAACAAA ACGAAACAAA ACAAACTAGC AAAATAGGCT GTCCCCAGTG CAAGTGCAGG TGCCAGAACA TTTCTCTATC GATA
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