pLV-Tet-miniCMV-HERC6(human)-GFP-nanobody-mCherry-Puro
$99.00
In stock
SKU
PL31805
NM_017912.4
100μg liquid plasmid(Instantly):$120
| name | pLV-Tet-miniCMV-HERC6(human)-GFP-nanobody-mCherry-Puro | another name | apLV-Tet-miniCMV-HERC6(human)-GFP-nanobody-mCherry-Puro |
| Insert Size (bp) | 3066bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression,Tetracycline induction | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Red |
| Promoter | Tet-miniCMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | miniCMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCAAATGGCA GTATTCATCC ACAATTTTAA AAGAAAAGGG GGGATTGGGG GGTACAGTGC AGGGGAAAGA ATAGTAGACA TAATAGCAAC AGACATACAA ACTAAAGAAT TACAAAAACA AATTACAAAA ATTCAAAATT TTCGGGTTTA TTACAGGGAC AGCAGAGAGA ATTCACGTGC TGCAGGTCCG AGGTTCTAGA CGAGTTTACT CCCTATCAGT GATAGAGAAC GATGTCGAGT TTACTCCCTA TCAGTGATAG AGAACGTATG TCGAGTTTAC TCCCTATCAG TGATAGAGAA CGTATGTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGTCGAGTTT ATCCCTATCA GTGATAGAGA ACGTATGTCG AGTTTACTCC CTATCAGTGA TAGAGAACGT ATGTCGAGGT AGGCGTGTAC GGTGGGAGGC CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGATCGCC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG ACTCACTATA GGGAGACCCA AGCTGTCAGA TCTCGAGCTC AAGCTTCGAA TTCTTCATTT ACTGCAGTGA ACTCCAAGCC ACCATGTACT TCTGTTGGGG CGCCGACTCC AGGGAGCTGC AGCGCCGGAG GACGGCGGGC AGCCCCGGGG CTGAGCTACT GCAGGCGGCC AGCGGGGAGC GCCACTCTCT GCTGCTGCTG ACCAACCACA GGGTCCTCTC GTGCGGAGAC AACAGCAGGG GTCAGCTGGG CCGCAGGGGC GCGCAGCGCG GGGAGCTGCC AGAACCAATT CAGGCATTGG AAACCCTAAT TGTTGATCTC GTGAGCTGCG GGAAGGAGCA CTCCCTGGCT GTGTGCCACA AAGGAAGGGT CTTCGCATGG GGAGCTGGTT CTGAAGGGCA GCTGGGGATT GGAGAATTCA AGGAAATAAG TTTCACACCT AAGAAAATAA TGACTCTGAA TGATATAAAA ATAATACAAG TTTCCTGTGG ACACTACCAC TCCCTGGCAT TATCAAAAGA TAGCCAAGTG TTTTCGTGGG GAAAGAACAG CCATGGGCAG CTGGGCTTGG GGAAGGAGTT CCCCTCCCAA GCCAGCCCGC AGAGGGTGAG GTCCCTGGAG GGGATCCCAC TGGCTCAGGT GGCTGCCGGA GGGGCTCACA GCTTTGCCCT GTCTCTCTGT GGGACTTCGT TTGGCTGGGG AAGTAACAGT GCCGGGCAGC TGGCCCTCAG TGGGCGTAAT GTCCCAGTGC AAAGCAACAA GCCTCTCTCA GTCGGTGCAC TGAAGAATCT AGGTGTGGTT TATATCAGCT GTGGTGATGC ACACACTGCG GTGCTTACCC AGGACGGGAA AGTGTTCACA TTTGGAGACA ATCGCTCTGG ACAGCTGGGA TACAGCCCCA CTCCTGAGAA GAGAGGTCCA CAACTTGTGG AAAGAATTGA TGGCCTAGTT TCGCAGATAG ATTGTGGAAG TTATCACACC CTGGCATATG TGCACACCAC TGGTCAGGTG GTATCTTTTG GTCATGGACC AAGTGACACA AGCAAGCCAA CTCATCCGGA GGCCCTGACA GAGAACTTTG ACATTAGCTG CCTGATTTCT GCTGAAGACT TCGTGGATGT TCAAGTCAAA CACATTTTTG CTGGAACATA TGCCAACTTT GTGACAACTC ATCAGGATAC TAGTTCCACA CGTGCTCCCG GGAAAACCCT GCCAGAAATA AGCCGAATTA GCCAGTCCAT GGCAGAAAAA TGGATAGCAG TGAAAAGAAG AAGTACTGAA CATGAAATGG CTAAAAGTGA AATTAGAATG ATATTTTCAT CTCCTGCTTG TCTGACTGCA AGTTTTTTAA AGAAAAGAGG AACTGGAGAA ACGACTTCCA TTGATGTGGA CTTAGAAATG GCAAGAGATA CCTTCAAGAA GTTAACAAAA AAGGAATGGA TTTCTTCCAT GATAACTACG TGTCTCGAGG ATGATCTGCT CAGAGCTCTT CCATGCCATT CTCCACACCA AGAAGCTTTA TCAGTTTTCC TCCTGCTCCC AGAATGTCCT GTGATGCATG ATTCTAAGAA CTGGAAGAAC CTGGTGGTTC CATTTGCAAA GGCTGTGTGT GAAATGAGTA AACAATCTTT GCAAGTCCTA AAGAAGTGTT GGGCATTTTT GCAAGAATCT TCTCTGAATC CGCTGATCCA GATGCTTAAA GCAGCCATCA TCTCTCAGCT GCTTCATCAG ACTAAAACCG AACAGGATCA CTGTAATGTT AAAGCTCTTT TAGGAATGAT GAAAGAACTG CATAAGGTAA ACAAAGCTAA CTGTCGACTA CCAGAAAATA CTTTCAACAT AAATGAACTC TCCAACTTAT TAAACTTTTA TATAGATAGA GGAAGACAGC TCTTTCGGGA TAACCACCTG ATACCTGCAG AAACCCCCAG TCCTGTTATT TTCAGTGATT TTCCATTTAT CTTTAATTCG CTATCCAAAA TTAAATTATT GCAAGCTGAT TCACATATAA AGATGCAGAT GTCAGAAAAG AAAGCATACA TGCTTATGCA TGAAACAATT CTGCAAAAAA AGGATGAATT TCCTCCATCA CCCAGATTTA TACTTAGAGT CAGACGAAGT CGCCTGGTTA AAGATGCTCT GCGTCAATTA AGTCAAGCTG AAGCTACTGA CTTCTGCAAA GTATTAGTGG TTGAATTTAT TAATGAAATT TGTCCTGAGT CTGGAGGGGT TAGTTCAGAG TTCTTCCACT GTATGTTTGA AGAGATGACC AAGCCAGAAT ATGGAATGTT CATGTATCCT GAAATGGGTT CCTGCATGTG GTTTCCTGCC AAGCCTAAAC CTGAGAAGAA AAGATATTTC CTCTTTGGAA TGCTGTGTGG ACTCTCCTTA TTCAATTTAA ATGTTGCTAA CCTTCCTTTC CCACTGGCTC TGTATAAAAA ACTTCTGGAC CAAAAGCCAT CATTGGAAGA TTTAAAAGAA CTCAGTCCTC GGTTGGGGAA GAGTTTGCAA GAAGTTCTAG ATGATGCTGC TGATGACATT GGAGATGCGC TCTGCATACG CTTTTCTATA CACTGGGACC AAAATGATGT TGACTTAATT CCAAATGGGA TCTCCATACC TGTGGACCAA ACCAACAAGA GAGACTATGT TTCTAAGTAT ATTGATTACA TTTTCAACGT CTCTGTAAAA GCAGTTTATG AGGAATTTCA GAGAGGATTT TATAGAGTCT GTGAGAAGGA GATACTTAGA CATTTCTACC CTGAAGAACT AATGACAGCA ATCATTGGAA ATACTGATTA TGACTGGAAA CAGTTTGAAC AGAATTCAAA GTATGAGCAA GGATACCAAA AATCACATCC TACTATACAG TTGTTTTGGA AGGCTTTCCA CAAACTAACC TTGGATGAAA AGAAAAAATT CCTCTTTTTC CTTACAGGAC GTGATAGGCT GCATGCAAGA GGCATACAGA AAATGGAAAT AGTATTTCGC TGTCCTGAAA CTTTCAGTGA AAGAGATCAC CCAACATCAA TAACTTGTCA TAATATTCTC TCCCTCCCTA AGTATTCTAC AATGGAAAGA ATGGAGGAAG CACTTCAAGT AGCCATCAAC AACAACAGAG GATTTGTCTC ACCCATGCTC ACACAGTCAT ACCCCTACGA CGTGCCTGAC TACGCCGGCG GCGGTGGTTC AGGCGGTGGC GGTTCCGGTG GTGGGGGATC AGTCCAACTG GTGGAGTCTG GTGGCGCTTT GGTGCAGCCA GGTGGCTCTC TGCGTTTGTC CTGTGCCGCT TCTGGCTTCC CAGTGAACCG CTATTCCATG CGCTGGTATC GCCAGGCTCC AGGCAAAGAG CGTGAGTGGG TAGCCGGTAT GTCCAGCGCG GGTGATCGTA GCTCCTATGA AGACTCCGTG AAGGGCCGTT TCACCATCAG CCGTGACGAT GCCCGTAACA CGGTGTATCT GCAAATGAAC AGCTTGAAAC CTGAAGATAC GGCCGTGTAT TACTGTAATG TGAACGTGGG CTTCGAGTAT TGGGGCCAAG GCACCCAGGT CACCGTCTCC AGCTAAGATC CTTGACTTGC GGCCGCAACT CCCACCTGCA ACATGCGTGA CTGACTGAGG CCGCGACTCT AGAGTCGACC TGCATCTAGG CGCCGGAATT AGATCTCTCG AGGTTAACGA ATTCTACCGG GTAGGGGAGG CGCTTTTCCC AAGGCAGTCT GGAGCATGCG CTTTAGCAGC CCCGCTGGGC ACTTGGCGCT ACACAAGTGG CCTCTGGCCT CGCACACATT CCACATCCAC CGGTAGGCGC CAACCGGCTC CGTTCTTTGG TGGCCCCTTC GCGCCACCTT CTACTCCTCC CCTAGTCAGG AAGTTCCCCC CCGCCCCGCA GCTCGCGTCG TGCAGGACGT GACAAATGGA AGTAGCACGT CTCACTAGTC TCGTGCAGAT GGACAGCACC GCTGAGCAAT GGAAGCGGGT AGGCCTTTGG GGCAGCGGCC AATAGCAGCT TTGCTCCTTC GCTTTCTGGG CTCAGAGGCT GGGAAGGGGT GGGTCCGGGG GCGGGCTCAG GGGCGGGCTC AGGGGCGGGG CGGGCGCCCG AAGGTCCTCC GGAGGCCCGG CATTCTGCAC GCTTCAAAAG CGCACGTCTG CCGCGCTGTT CTCCTCTTCC TCATCTCCGG GCCTTTCGAC CTGCAGCCCA AGCTTACCAT GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCTGCCCTT CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG TGAAGCACCC CGCCGACATC CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT GCAGAAGAAG ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA CCGGCGGCAT GGACGAGCTG TACAAGGCAA CAAACTTCTC TCTGCTGAAA CAAGCCGGAG ATGTCGAAGA GAATCCTGGA CCGATGACCG AGTACAAGCC CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGATCTTATC GATAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAAT CATCGTCCTT TCCTTGGCTG CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCATCGATAC CGTCGACCAA GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAGGGG GGACTGGAAG GGCTAATTCA CTCCCAACGA AGACAAGATC TGCTTTTTGC TTGTACTGGG TCTCTCTGGT TAGACCAGAT CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGC ATCTAGAATT AATTCCGTGT ATTCTATAGT GTCACCTAAA TCGTATGTGT ATGATACATA AGGTTATGTA TTAATTGTAG CCGCGTTCTA ACGACAATAT GTACAAGCCT AATTGTGTAG CATCTGGCTT ACTGAAGCAG ACCCTATCAT CTCTCTCGTA AACTGCCGTC AGAGTCGGTT TGGTTGGACG AACCTTCTGA GTTTCTGGTA ACGCCGTCCC GCACCCGGAA ATGGTCAGCG AACCAATCAG CAGGGTCATC GCTAGCCAGA TCCTCTACGC CGGACGCATC GTGGCCGGCA TCACCGGCGC CACAGGTGCG GTTGCTGGCG CCTATATCGC CGACATCACC GATGGGGAAG ATCGGGCTCG CCACTTCGGG CTCATGAGCG CTTGTTTCGG CGTGGGTATG GTGGCAGGCC CCGTGGCCGG GGGACTGTTG GGCGCCATCT CCTTGCATGC ACCATTCCTT GCGGCGGCGG TGCTCAACGG CCTCAACCTA CTACTGGGCT GCTTCCTAAT GCAGGAGTCG CATAAGGGAG AGCGTCGAAT GGTGCACTCT CAGTACAATC TAGCTCTGAT GCCGCATAGT TAAGCCAGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTTGGACA CAAGACAGGC TTGCGAGATA TGTTTGAGAA TACCACTTTA TCCCGCGTCA GGGAGAGGCA GTGCGTAAAA AGACGCGGAC TCATGTGAAA TACTGGTTTT TAGTGCGCCA GATCTCTATA ATCTCGCGCA ACCTATTTTC CCCTCGAACA CTTTTTAAGC CGTAGATAAA CAGGCTGGGA CACTTCACAT GAGCGAAAAA TACATCGTCA CCTGGGACAT GTTGCAGATC CATGCACGTA AACTCGCAAG CCGACTGATG CCTTCTGAAC AATGGAAAGG CATTATTGCC GTAAGCCGTG GCGGTCTGTA CCGGGTGCGT TACTGGCGCG TGAACTGGGT ATTCGTCATG TCGATACCGT TTGTATTTCC AGCTACGATC ACGACAACCA GCGCGAGCTT AAAGTGCTGA AACGCGCAGA AGGCGATGGC GAAGGCTTCA TCGTTATTGA TGACCTGGTG GATACCGGTG GTACTGCGGT TGCGATTCGT GAAATGTATC CAAAAGCGCA CTTTGTCACC ATCTTCGCAA AACCGGCTGG TCGTCCGCTG GTTGATGACT ATGTTGTTGA TATCCCGCAA GATACCTGGA TTGAACAGCC GTGGGATATG GGCGTCGTAT TCGTCCCGCC AATCTCCGGT CGCTAATCTT TTCAACGCCT GGCACTGCCG GGCGTTGTTC TTTTTAACTT CAGGCGGGTT ACAATAGTTT CCAGTAAGTA TTCTGGAGGC TGCATCCATG ACACAGGCAA ACCTGAGCGA AACCCTGTTC AAACCCCGCT TTAAACATCC TGAAACCTCG ACGCTAGTCC GCCGCTTTAA TCACGGCGCA CAACCGCCTG TGCAGTCGGC CCTTGATGGT AAAACCATCC CTCACTGGTA TCGCATGATT AACCGTCTGA TGTGGATCTG GCGCGGCATT GACCCACGCG AAATCCTCGA CGTCCAGGCA CGTATTGTGA TGAGCGATGC CGAACGTACC GACGATGATT TATACGATAC GGTGATTGGC TACCGTGGCG GCAACTGGAT TTATGAGTGG GCCCCGGATC TTTGTGAAGG AACCTTACTT CTGTGGTGTG ACATAATTGG ACAAACTACC TACAGAGATT TAAAGCTCTA AGGTAAATAT AAAATTTTTA AGTGTATAAT GTGTTAAACT ACTGATTCTA ATTGTTTGTG TATTTTAGAT TCCAACCTAT GGAACTGATG AATGGGAGCA GTGGTGGAAT GCCTTTAATG AGGAAAACCT GTTTTGCTCA GAAGAAATGC CATCTAGTGA TGATGAGGCT ACTGCTGACT CTCAACATTC TACTCCTCCA AAAAAGAAGA GAAAGGTAGA AGACCCCAAG GACTTTCCTT CAGAATTGCT AAGTTTTTTG AGTCATGCTG TGTTTAGTAA TAGAACTCTT GCTTGCTTTG CTATTTACAC CACAAAGGAA AAAGCTGCAC TGCTATACAA GAAAATTATG GAAAAATATT CTGTAACCTT TATAAGTAGG CATAACAGTT ATAATCATAA CATACTGTTT TTTCTTACTC CACACAGGCA TAGAGTGTCT GCTATTAATA ACTATGCTCA AAAATTGTGT ACCTTTAGCT TTTTAATTTG TAAAGGGGTT AATAAGGAAT ATTTGATGTA TAGTGCCTTG ACTAGAGATC ATAATCAGCC ATACCACATT TGTAGAGGTT TTACTTGCTT TAAAAAACCT CCCACACCTC CCCCTGAACC TGAAACATAA AATGAATGCA ATTGTTGTTG TTAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGGATCAAC TGGATAACTC AAGCTAACCA AAATCATCCC AAACTTCCCA CCCCATACCC TATTACCACT GCCAATTACC TAGTGGTTTC ATTTACTCTA AACCTGTGAT TCCTCTGAAT TATTTTCATT TTAAAGAAAT TGTATTTGTT AAATATGTAC TACAAACTTA GTAGTTGGAA GGGCTAATTC ACTCCCAAAG AAGACAAGAT ATCCTTGATC TGTGGATCTA CCACACACAA GGCTACTTCC CTGATTAGCA GAACTACACA CCAGGGCCAG GGGTCAGATA TCCACTGACC TTTGGATGGT GCTACAAGCT AGTACCAGTT GAGCCAGATA AGGTAGAAGA GGCCAATAAA GGAGAGAACA CCAGCTTGTT ACACCCTGTG AGCCTGCATG GGATGGATGA CCCGGAGAGA GAAGTGTTAG AGTGGAGGTT TGACAGCCGC CTAGCATTTC ATCACGTGGC CCGAGAGCTG CATCCGGAGT ACTTCAAGAA CTGCTGATAT CGAGCTTGCT ACAAGGGACT TTCCGCTGGG GACTTTCCAG GGAGGCGTGG CCTGGGCGGG ACTGGGGAGT GGCGAGCCCT CAGATCCTGC ATATAAGCAG CTGCTTTTTG CCTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG TGGCGCCCGA ACAGGGACTT GAAAGCGAAA GGGAAACCAG AGGAGCTCTC TCGACGCAGG ACTCGGCTTG CTGAAGCGCG CACGGCAAGA GGCGAGGGGC GGCGACTGGT GAGTACGCCA AAAATTTTGA CTAGCGGAGG CTAGAAGGAG AGAGATGGGT GCGAGAGCGT CAGTATTAAG CGGGGGAGAA TTAGATCGCG ATGGGAAAAA ATTCGGTTAA GGCCAGGGGG AAAGAAAAAA TATAAATTAA AACATATAGT ATGGGCAAGC AGGGAGCTAG AACGATTCGC AGTTAATCCT GGCCTGTTAG AAACATCAGA AGGCTGTAGA CAAATACTGG GACAGCTACA ACCATCCCTT CAGACAGGAT CAGAAGAACT TAGATCATTA TATAATACAG TAGCAACCCT CTATTGTGTG CATCAAAGGA TAGAGATAAA AGACACCAAG GAAGCTTTAG ACAAGATAGA GGAAGAGCAA AACAAAAGTA AGACCACCGC ACAGCAAGCG GCCGGTGATC TTCAGACCTG GACGATATAT ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCTCGACGG TCG
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