NM_032807.5;Fbx18; FBXO18; hFBH1
100μg liquid plasmid(Instantly):$120
| name | pLV-Tet-miniCMV-FBH1(human)-GFP-nanobody-mCherry-Puro | another name | apLV-Tet-miniCMV-FBH1(human)-GFP-nanobody-mCherry-Puro |
| Insert Size (bp) | 3282bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression,Tetracycline induction | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Red |
| Promoter | Tet-miniCMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | miniCMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCAAATGGCA GTATTCATCC ACAATTTTAA AAGAAAAGGG GGGATTGGGG GGTACAGTGC AGGGGAAAGA ATAGTAGACA TAATAGCAAC AGACATACAA ACTAAAGAAT TACAAAAACA AATTACAAAA ATTCAAAATT TTCGGGTTTA TTACAGGGAC AGCAGAGAGA ATTCACGTGC TGCAGGTCCG AGGTTCTAGA CGAGTTTACT CCCTATCAGT GATAGAGAAC GATGTCGAGT TTACTCCCTA TCAGTGATAG AGAACGTATG TCGAGTTTAC TCCCTATCAG TGATAGAGAA CGTATGTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGTCGAGTTT ATCCCTATCA GTGATAGAGA ACGTATGTCG AGTTTACTCC CTATCAGTGA TAGAGAACGT ATGTCGAGGT AGGCGTGTAC GGTGGGAGGC CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGATCGCC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG ACTCACTATA GGGAGACCCA AGCTGTCAGA TCTCGAGCTC AAGCTTCGAA TTCTTTGATC GTCGCAGTGA ACTCCAAGCC ACCATGAGCT ACGAGGTGAC TTCAGGCTGC CATTGGACCT GTCAAGTGCC TGAGTCATGT GATAATGGGC TACATTGCGC AGGGCCCCTG GGCCATCTCC ACAGGAGATG CCAGAGGACG AGTGCCCACT TGCTGGTCTT CACAGAGCAC GCTGAAATGA GACGGTTTAA GCGGAAGCAT CTTACTGCCA TTGACTGCCA GCATTTGGCT CGGAGTCACT TGGCTGTGAC CCAGCCCTTC GGTCAAAGAT GGACAAACAG AGATCCGAAC CATGGTCTCT ATCCTAAACC GAGAACAAAA AGAGGGAGTA GGGGTCAGGG AAGTCAAAGA TGCATCCCTG AGTTCTTCCT AGCAGGCAAG CAGCCGTGCA CCAATGACAT GGCCAAAAGC AATTCTGTTG GCCAGGACAG CTGTCAGGAC TCTGAGGGTG ACATGATCTT TCCTGCAGAG AGCAGCTGTG CACTGCCTCA GGAAGGCAGT GCAGGGCCGG GCTCACCAGG GTCTGCCCCG CCCTCCAGGA AGCGGTCTTG GTCCTCTGAG GAAGAGAGTA ACCAGGCTAC CGGGACCAGC CGGTGGGATG GAGTTTCTAA GAAAGCTCCA CGGCACCATT TGTCTGTGCC ATGCACAAGG CCTAGGGAGG CCAGGCAAGA AGCAGAGGAC AGTACGTCTC GGCTCTCTGC GGAGTCTGGT GAAACCGACC AAGATGCTGG GGACGTGGGT CCTGATCCCA TTCCTGACTC ATACTATGGG CTTCTTGGGA CCTTGCCCTG CCAGGAAGCA CTGAGCCACA TTTGCAGCCT GCCTAGTGAG GTCCTGAGGC ACGTGTTTGC CTTCCTCCCG GTGGAAGACC TCTATTGGAA CCTGAGCTTG GTGTGCCACT TGTGGAGGGA GATCATCAGT GACCCGCTGT TCATTCCTTG GAAGAAGCTG TACCATCGAT ACCTGATGAA TGAAGAGCAA GCTGTCAGCA AAGTGGACGG CATCCTGTCT AACTGTGGCA TAGAAAAGGA GTCAGACCTG TGTGTGCTGA ACCTCATACG ATACACAGCC ACCACTAAGT GCTCTCCGAG TGTGGATCCC GAGAGGGTGC TGTGGAGTCT GAGGGACCAC CCCCTCCTCC CCGAGGCTGA GGCGTGTGTG CGGCAACACC TCCCCGACCT CTACGCTGCT GCCGGGGGTG TCAACATCTG GGCCCTGGTG GCGGCTGTGG TGCTCCTCTC CAGCAGTGTG AATGACATCC AGCGACTGCT CTTCTGCCTC CGGAGACCCA GCTCCACGGT GACCATGCCA GATGTCACCG AGACCCTGTA CTGCATAGCC GTGCTTCTCT ACGCCATGAG GGAGAAGGGG ATTAACATCA GCAATAGGAT TCACTACAAC ATTTTCTATT GCCTATATCT TCAGGAGAAT TCCTGCACTC AGGCCACAAA AGTTAAAGAG GAGCCATCTG TCTGGCCAGG CAAGAAAACC ATCCAACTTA CACATGAACA ACAGCTGATT CTGAATCACA AGATGGAACC TCTCCAGGTG GTGAAAATTA TGGCCTTTGC CGGCACTGGG AAGACCTCAA CGCTGGTCAA GTATGCAGAG AAGTGGTCTC AGAGCAGGTT TCTGTATGTG ACATTCAACA AGAGCATCGC AAAGCAGGCC GAACGCGTCT TCCCCAGCAA CGTCATCTGC AAAACCTTCC ACTCCATGGC CTACGGGCAC ATAGGGCGGA AGTACCAGTC AAAGAAGAAG TTGAATCTCT TCAAGTTAAC ACCCTTCATG GTCAACTCCG TCCTTGCTGA AGGGAAGGGT GGATTCATAA GAGCCAAGCT TGTGTGTAAG ACTCTAGAAA ACTTCTTTGC CTCGGCTGAC GAAGAGCTGA CCATTGATCA CGTGCCTATT TGGTGTAAGA ACAGCCAAGG ACAGAGAGTC ATGGTTGAGC AGAGTGAAAA ACTGAATGGT GTCCTTGAAG CGAGCCGCCT CTGGGATAAC ATGCGGAAGC TGGGGGAGTG CACAGAAGAG GCGCACCAGA TGACTCATGA CGGCTACTTG AAACTCTGGC AGCTGAGCAA GCCTTCGCTG GCCTCTTTTG ACGCCATCTT TGTGGATGAG GCCCAGGACT GCACACCAGC TATCATGAAC ATAGTTCTGT CTCAGCCATG TGGGAAAATC TTTGTAGGGG ACCCGCACCA GCAGATCTAT ACCTTCCGGG GTGCGGTCAA CGCCCTGTTC ACAGTGCCCC ACACCCACGT CTTCTATCTC ACGCAGAGTT TTCGGTTTGG TGTGGAAATA GCTTATGTGG GAGCTACTAT CTTGGATGTT TGCAAGAGAG TCAGGAAAAA GACTTTGGTT GGAGGAAACC ATCAGAGTGG CATTAGAGGT GACGCAAAGG GGCAAGTGGC CTTGTTGTCC CGGACCAACG CCAACGTGTT TGATGAGGCC GTACGGGTGA CGGAAGGGGA ATTCCCTTCA AGGATACATT TGATTGGGGG GATTAAATCA TTTGGATTGG ACAGAATCAT TGATATTTGG ATCCTTCTTC AGCCAGAGGA AGAACGGAGG AAACAAAACC TCGTCATTAA AGACAAATTT ATCAGAAGAT GGGTGCACAA AGAAGGCTTT AGTGGCTTCA AGAGGTATGT GACCGCTGCC GAGGACAAGG AGCTTGAAGC CAAGATCGCA GTTGTTGAAA AGTATAACAT CAGGATTCCA GAGCTGGTGC AAAGGATAGA AAAATGCCAT ATAGAAGATT TGGACTTTGC AGAGTACATT CTGGGCACTG TGCACAAAGC CAAAGGCCTG GAGTTTGACA CTGTGCATGT TTTGGATGAT TTTGTGAAAG TGCCTTGTGC CCGGCATAAC CTGCCCCAGC TTCCGCACTT CAGAGTTGAG TCATTTTCTG AGGATGAATG GAATTTACTG TATGTTGCAG TAACTCGAGC CAAGAAGCGT CTCATCATGA CCAAATCATT GGAAAACATT TTGACTTTGG CTGGGGAGTA CTTCTTGCAA GCAGAGCTGA CAAGCAACGT CTTAAAAACA GGCGTGGTGC GCTGCTGCGT GGGACAGTGC AACAATGCCA TCCCTGTTGA CACCGTCCTT ACCATGAAGA AGCTGCCCAT CACCTATAGC AACAGGAAGG AAAACAAGGG GGGCTACCTC TGCCACTCCT GTGCGGAGCA GCGCATCGGG CCCCTGGCGT TCCTGACAGC CTCCCCGGAG CAGGTGCGCG CCATGGAGCG CACTGTGGAG AACATCGTAC TGCCCCGGCA TGAGGCCCTG CTCTTCCTCG TCTTCTACCC CTACGACGTG CCTGACTACG CCGGCGGCGG TGGTTCAGGC GGTGGCGGTT CCGGTGGTGG GGGATCAGTC CAACTGGTGG AGTCTGGTGG CGCTTTGGTG CAGCCAGGTG GCTCTCTGCG TTTGTCCTGT GCCGCTTCTG GCTTCCCAGT GAACCGCTAT TCCATGCGCT GGTATCGCCA GGCTCCAGGC AAAGAGCGTG AGTGGGTAGC CGGTATGTCC AGCGCGGGTG ATCGTAGCTC CTATGAAGAC TCCGTGAAGG GCCGTTTCAC CATCAGCCGT GACGATGCCC GTAACACGGT GTATCTGCAA ATGAACAGCT TGAAACCTGA AGATACGGCC GTGTATTACT GTAATGTGAA CGTGGGCTTC GAGTATTGGG GCCAAGGCAC CCAGGTCACC GTCTCCAGCT AAGATCCTTG ACTTGCGGCC GCAACTCCCA CCTGCAACAT GCGTGACTGA CTGAGGCCGC GACTCTAGAG TCGACCTGCA TCTAGGCGCC GGAATTAGAT CTCTCGAGGT TAACGAATTC TACCGGGTAG GGGAGGCGCT TTTCCCAAGG CAGTCTGGAG CATGCGCTTT AGCAGCCCCG CTGGGCACTT GGCGCTACAC AAGTGGCCTC TGGCCTCGCA CACATTCCAC ATCCACCGGT AGGCGCCAAC CGGCTCCGTT CTTTGGTGGC CCCTTCGCGC CACCTTCTAC TCCTCCCCTA GTCAGGAAGT TCCCCCCCGC CCCGCAGCTC GCGTCGTGCA GGACGTGACA AATGGAAGTA GCACGTCTCA CTAGTCTCGT GCAGATGGAC AGCACCGCTG AGCAATGGAA GCGGGTAGGC CTTTGGGGCA GCGGCCAATA GCAGCTTTGC TCCTTCGCTT TCTGGGCTCA GAGGCTGGGA AGGGGTGGGT CCGGGGGCGG GCTCAGGGGC GGGCTCAGGG GCGGGGCGGG CGCCCGAAGG TCCTCCGGAG GCCCGGCATT CTGCACGCTT CAAAAGCGCA CGTCTGCCGC GCTGTTCTCC TCTTCCTCAT CTCCGGGCCT TTCGACCTGC AGCCCAAGCT TACCATGGTG AGCAAGGGCG AGGAGGATAA CATGGCCATC ATCAAGGAGT TCATGCGCTT CAAGGTGCAC ATGGAGGGCT CCGTGAACGG CCACGAGTTC GAGATCGAGG GCGAGGGCGA GGGCCGCCCC TACGAGGGCA CCCAGACCGC CAAGCTGAAG GTGACCAAGG GTGGCCCCCT GCCCTTCGCC TGGGACATCC TGTCCCCTCA GTTCATGTAC GGCTCCAAGG CCTACGTGAA GCACCCCGCC GACATCCCCG ACTACTTGAA GCTGTCCTTC CCCGAGGGCT TCAAGTGGGA GCGCGTGATG AACTTCGAGG ACGGCGGCGT GGTGACCGTG ACCCAGGACT CCTCCCTGCA GGACGGCGAG TTCATCTACA AGGTGAAGCT GCGCGGCACC AACTTCCCCT CCGACGGCCC CGTAATGCAG AAGAAGACCA TGGGCTGGGA GGCCTCCTCC GAGCGGATGT ACCCCGAGGA CGGCGCCCTG AAGGGCGAGA TCAAGCAGAG GCTGAAGCTG AAGGACGGCG GCCACTACGA CGCTGAGGTC AAGACCACCT ACAAGGCCAA GAAGCCCGTG CAGCTGCCCG GCGCCTACAA CGTCAACATC AAGTTGGACA TCACCTCCCA CAACGAGGAC TACACCATCG TGGAACAGTA CGAACGCGCC GAGGGCCGCC ACTCCACCGG CGGCATGGAC GAGCTGTACA AGGCAACAAA CTTCTCTCTG CTGAAACAAG CCGGAGATGT CGAAGAGAAT CCTGGACCGA TGACCGAGTA CAAGCCCACG GTGCGCCTCG CCACCCGCGA CGACGTCCCC AGGGCCGTAC GCACCCTCGC CGCCGCGTTC GCCGACTACC CCGCCACGCG CCACACCGTC GATCCGGACC GCCACATCGA GCGGGTCACC GAGCTGCAAG AACTCTTCCT CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG GACGACGGCG CCGCGGTGGC GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG TTCGCCGAGA TCGGCCCGCG CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA CAGATGGAAG GCCTCCTGGC GCCGCACCGG CCCAAGGAGC CCGCGTGGTT CCTGGCCACC GTCGGCGTCT CGCCCGACCA CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT GCTCCCCGGA GTGGAGGCGG CCGAGCGCGC CGGGGTGCCC GCCTTCCTGG AGACCTCCGC GCCCCGCAAC CTCCCCTTCT ACGAGCGGCT CGGCTTCACC GTCACCGCCG ACGTCGAGGT GCCCGAAGGA CCGCGCACCT GGTGCATGAC CCGCAAGCCC GGTGCCTGAT CTTATCGATA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAATCATC GTCCTTTCCT TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCAT CGATACCGTC GACCAAGGCA GCTGTAGATC TTAGCCACTT TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT AATTCACTCC CAACGAAGAC AAGATCTGCT TTTTGCTTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGCATCT AGAATTAATT CCGTGTATTC TATAGTGTCA CCTAAATCGT ATGTGTATGA TACATAAGGT TATGTATTAA TTGTAGCCGC GTTCTAACGA CAATATGTAC AAGCCTAATT GTGTAGCATC TGGCTTACTG AAGCAGACCC TATCATCTCT CTCGTAAACT GCCGTCAGAG TCGGTTTGGT TGGACGAACC TTCTGAGTTT CTGGTAACGC CGTCCCGCAC CCGGAAATGG TCAGCGAACC AATCAGCAGG GTCATCGCTA GCCAGATCCT CTACGCCGGA CGCATCGTGG CCGGCATCAC CGGCGCCACA GGTGCGGTTG CTGGCGCCTA TATCGCCGAC ATCACCGATG GGGAAGATCG GGCTCGCCAC TTCGGGCTCA TGAGCGCTTG TTTCGGCGTG GGTATGGTGG CAGGCCCCGT GGCCGGGGGA CTGTTGGGCG CCATCTCCTT GCATGCACCA TTCCTTGCGG CGGCGGTGCT CAACGGCCTC AACCTACTAC TGGGCTGCTT CCTAATGCAG GAGTCGCATA AGGGAGAGCG TCGAATGGTG CACTCTCAGT ACAATCTAGC TCTGATGCCG CATAGTTAAG CCAGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT TGGACACAAG ACAGGCTTGC GAGATATGTT TGAGAATACC ACTTTATCCC GCGTCAGGGA GAGGCAGTGC GTAAAAAGAC GCGGACTCAT GTGAAATACT GGTTTTTAGT GCGCCAGATC TCTATAATCT CGCGCAACCT ATTTTCCCCT CGAACACTTT TTAAGCCGTA GATAAACAGG CTGGGACACT TCACATGAGC GAAAAATACA TCGTCACCTG GGACATGTTG CAGATCCATG CACGTAAACT CGCAAGCCGA CTGATGCCTT CTGAACAATG GAAAGGCATT ATTGCCGTAA GCCGTGGCGG TCTGTACCGG GTGCGTTACT GGCGCGTGAA CTGGGTATTC GTCATGTCGA TACCGTTTGT ATTTCCAGCT ACGATCACGA CAACCAGCGC GAGCTTAAAG TGCTGAAACG CGCAGAAGGC GATGGCGAAG GCTTCATCGT TATTGATGAC CTGGTGGATA CCGGTGGTAC TGCGGTTGCG ATTCGTGAAA TGTATCCAAA AGCGCACTTT GTCACCATCT TCGCAAAACC GGCTGGTCGT CCGCTGGTTG ATGACTATGT TGTTGATATC CCGCAAGATA CCTGGATTGA ACAGCCGTGG GATATGGGCG TCGTATTCGT CCCGCCAATC TCCGGTCGCT AATCTTTTCA ACGCCTGGCA CTGCCGGGCG TTGTTCTTTT TAACTTCAGG CGGGTTACAA TAGTTTCCAG TAAGTATTCT GGAGGCTGCA TCCATGACAC AGGCAAACCT GAGCGAAACC CTGTTCAAAC CCCGCTTTAA ACATCCTGAA ACCTCGACGC TAGTCCGCCG CTTTAATCAC GGCGCACAAC CGCCTGTGCA GTCGGCCCTT GATGGTAAAA CCATCCCTCA CTGGTATCGC ATGATTAACC GTCTGATGTG GATCTGGCGC GGCATTGACC CACGCGAAAT CCTCGACGTC CAGGCACGTA TTGTGATGAG CGATGCCGAA CGTACCGACG ATGATTTATA CGATACGGTG ATTGGCTACC GTGGCGGCAA CTGGATTTAT GAGTGGGCCC CGGATCTTTG TGAAGGAACC TTACTTCTGT GGTGTGACAT AATTGGACAA ACTACCTACA GAGATTTAAA GCTCTAAGGT AAATATAAAA TTTTTAAGTG TATAATGTGT TAAACTACTG ATTCTAATTG TTTGTGTATT TTAGATTCCA ACCTATGGAA CTGATGAATG GGAGCAGTGG TGGAATGCCT TTAATGAGGA AAACCTGTTT TGCTCAGAAG AAATGCCATC TAGTGATGAT GAGGCTACTG CTGACTCTCA ACATTCTACT CCTCCAAAAA AGAAGAGAAA GGTAGAAGAC CCCAAGGACT TTCCTTCAGA ATTGCTAAGT TTTTTGAGTC ATGCTGTGTT TAGTAATAGA ACTCTTGCTT GCTTTGCTAT TTACACCACA AAGGAAAAAG CTGCACTGCT ATACAAGAAA ATTATGGAAA AATATTCTGT AACCTTTATA AGTAGGCATA ACAGTTATAA TCATAACATA CTGTTTTTTC TTACTCCACA CAGGCATAGA GTGTCTGCTA TTAATAACTA TGCTCAAAAA TTGTGTACCT TTAGCTTTTT AATTTGTAAA GGGGTTAATA AGGAATATTT GATGTATAGT GCCTTGACTA GAGATCATAA TCAGCCATAC CACATTTGTA GAGGTTTTAC TTGCTTTAAA AAACCTCCCA CACCTCCCCC TGAACCTGAA ACATAAAATG AATGCAATTG TTGTTGTTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGG ATCAACTGGA TAACTCAAGC TAACCAAAAT CATCCCAAAC TTCCCACCCC ATACCCTATT ACCACTGCCA ATTACCTAGT GGTTTCATTT ACTCTAAACC TGTGATTCCT CTGAATTATT TTCATTTTAA AGAAATTGTA TTTGTTAAAT ATGTACTACA AACTTAGTAG TTGGAAGGGC TAATTCACTC CCAAAGAAGA CAAGATATCC TTGATCTGTG GATCTACCAC ACACAAGGCT ACTTCCCTGA TTAGCAGAAC TACACACCAG GGCCAGGGGT CAGATATCCA CTGACCTTTG GATGGTGCTA CAAGCTAGTA CCAGTTGAGC CAGATAAGGT AGAAGAGGCC AATAAAGGAG AGAACACCAG CTTGTTACAC CCTGTGAGCC TGCATGGGAT GGATGACCCG GAGAGAGAAG TGTTAGAGTG GAGGTTTGAC AGCCGCCTAG CATTTCATCA CGTGGCCCGA GAGCTGCATC CGGAGTACTT CAAGAACTGC TGATATCGAG CTTGCTACAA GGGACTTTCC GCTGGGGACT TTCCAGGGAG GCGTGGCCTG GGCGGGACTG GGGAGTGGCG AGCCCTCAGA TCCTGCATAT AAGCAGCTGC TTTTTGCCTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGTGGC GCCCGAACAG GGACTTGAAA GCGAAAGGGA AACCAGAGGA GCTCTCTCGA CGCAGGACTC GGCTTGCTGA AGCGCGCACG GCAAGAGGCG AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA TTTTGACTAG CGGAGGCTAG AAGGAGAGAG ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG GGAGAATTAG ATCGCGATGG GAAAAAATTC GGTTAAGGCC AGGGGGAAAG AAAAAATATA AATTAAAACA TATAGTATGG GCAAGCAGGG AGCTAGAACG ATTCGCAGTT AATCCTGGCC TGTTAGAAAC ATCAGAAGGC TGTAGACAAA TACTGGGACA GCTACAACCA TCCCTTCAGA CAGGATCAGA AGAACTTAGA TCATTATATA ATACAGTAGC AACCCTCTAT TGTGTGCATC AAAGGATAGA GATAAAAGAC ACCAAGGAAG CTTTAGACAA GATAGAGGAA GAGCAAAACA AAAGTAAGAC CACCGCACAG CAAGCGGCCG GTGATCTTCA GACCTGGACG ATATATATGA GGGACAATTG GAGAAGTGAA TTATATAAAT ATAAAGTAGT AAAAATTGAA CCATTAGGAG TAGCACCCAC CAAGGCAAAG AGAAGAGTGG TGCAGAGAGA AAAAAGAGCA GTGGGAATAG GAGCTTTGTT CCTTGGGTTC TTGGGAGCAG CAGGAAGCAC TATGGGCGCA GCGTCAATGA CGCTGACGGT ACAGGCCAGA CAATTATTGT CTGGTATAGT GCAGCAGCAG AACAATTTGC TGAGGGCTAT TGAGGCGCAA CAGCATCTGT TGCAACTCAC AGTCTGGGGC ATCAAGCAGC TCCAGGCAAG AATCCTGGCT GTGGAAAGAT ACCTAAAGGA TCAACAGCTC CTGGGGATTT GGGGTTGCTC TGGAAAACTC ATTTGCACCA CTGCTGTGCC TTGGAATGCT AGTTGGAGTA ATAAATCTCT GGAACAGATT TGGAATCACA CGACCTGGAT GGAGTGGGAC AGAGAAATTA ACAATTACAC AAGCTTAATA CACTCCTTAA TTGAAGAATC GCAAAACCAG CAAGAAAAGA ATGAACAAGA ATTATTGGAA TTAGATAAAT GGGCAAGTTT GTGGAATTGG TTTAACATAA CAAATTGGCT GTGGTATATA AAATTATTCA TAATGATAGT AGGAGGCTTG GTAGGTTTAA GAATAGTTTT TGCTGTACTT TCTATAGTGA ATAGAGTTAG GCAGGGATAT TCACCATTAT CGTTTCAGAC CCACCTCCCA ACCCCGAGGG GACCCGACAG GCCCGAAGGA ATAGAAGAAG AAGGTGGAGA GAGAGACAGA GACAGATCCA TTCGATTAGT GAACGGATCT CGACGGTCG
