pPR3-C-ACE2(human)
$84.00
In stock
SKU
PL30453
NM_001371415.1;ACEH
100μg liquid plasmid(Instantly):$105
| name | pPR3-C-ACE2(human) | another name | apPR3-C-ACE2(human) |
| Insert Size (bp) | 2418bp | Plasmid host | Yeast bacterium |
| Plasmid uses | hybridization | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Trp | Fluorescent labeling | |
| Promoter | ADH | Replicon | 2U |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pPR3-C | 5′ sequencing primer | ADH1-F1：GCTATACCAAGCATACAATCAAC |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCCGCGGATG ATTACGCCAA GCGCGCAATT AACCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCCGCGGATG ATTACGCCAA GCGCGCAATT AACCCTCACT AAAGGGAACA AAAGCTGGAG CTCATATCCT TTTGTTGTTT CCGGGTGTAC AATATGGACT TCCTCTTTTC TGGCAACCAA ACCCATACAT CGGGATTCCT ATAATACCTT CGTTGGTCTC CCTAACATGT AGGTGGCGGA GGGGAGATAT ACAATAGAAC AGATACCAGA CAAGACATAA TGGGCTAAAC AAGACTACAC CAATTACACT GCCTCATTGA TGGTGGTACA TAACGAACTA ATACTGTAGC CCTAGACTTG ATAGCCATCA TCATATCGAA GTTTCACTAC CCTTTTTCCA TTTGCCATCT ATTGAAGTAA TAATAGGCGC ATGCAACTTC TTTTCTTTTT TTTTCTTTTC TCTCTCCCCC GTTGTTGTCT CACCATATCC GCAATGACAA AAAAATGATG GAAGACACTA AAGGAAAAAA TTAACGACAA AGACAGCACC AACAGATGTC GTTGTTCCAG AGCTGATGAG GGGTATCTCG AAGCACACGA AACTTTTTCC TTCCTTCATT CACGCACACT ACTCTCTAAT GAGCAACGGT ATACGGCCTT CCTTCCAGTT ACTTGAATTT GAAATAAAAA AAAGTTTGCT GTCTTGCTAT CAAGTATAAA TAGACCTGCA ATTATTAATC TTTTGTTTCC TCGTCATTGT TCTCGTTCCC TTTCTTCCTT GTTTCTTTTT CTGCACAATA TTTCAAGCTA TACCAAGCAT ACAATCAACT CACTAGTGGA TCCAAGCAGT GGTATCAACG CAGAGTGGCC ATTACGGCCC GGGAAAAAAC ATGTCGGCCG CCTCGGCCTC TCGAGAATTC GATATCAAGC TTATCGATAT GTACCCATAC GATGTTCCAG ATTACGCTCC TGCAGCCATG TCAAGCTCTT CCTGGCTCCT TCTCAGCCTT GTTGCTGTAA CTGCTGCTCA GTCCACCATT GAGGAACAGG CCAAGACATT TTTGGACAAG TTTAACCACG AAGCCGAAGA CCTGTTCTAT CAAAGTTCAC TTGCTTCTTG GAATTATAAC ACCAATATTA CTGAAGAGAA TGTCCAAAAC ATGAATAATG CTGGGGACAA ATGGTCTGCC TTTTTAAAGG AACAGTCCAC ACTTGCCCAA ATGTATCCAC TACAAGAAAT TCAGAATCTC ACAGTCAAGC TTCAGCTGCA GGCTCTTCAG CAAAATGGGT CTTCAGTGCT CTCAGAAGAC AAGAGCAAAC GGTTGAACAC AATTCTAAAT ACAATGAGCA CCATCTACAG TACTGGAAAA GTTTGTAACC CAGATAATCC ACAAGAATGC TTATTACTTG AACCAGGTTT GAATGAAATA ATGGCAAACA GTTTAGACTA CAATGAGAGG CTCTGGGCTT GGGAAAGCTG GAGATCTGAG GTCGGCAAGC AGCTGAGGCC ATTATATGAA GAGTATGTGG TCTTGAAAAA TGAGATGGCA AGAGCAAATC ATTATGAGGA CTATGGGGAT TATTGGAGAG GAGACTATGA AGTAAATGGG GTAGATGGCT ATGACTACAG CCGCGGCCAG TTGATTGAAG ATGTGGAACA TACCTTTGAA GAGATTAAAC CATTATATGA ACATCTTCAT GCCTATGTGA GGGCAAAGTT GATGAATGCC TATCCTTCCT ATATCAGTCC AATTGGATGC CTCCCTGCTC ATTTGCTTGG TGATATGTGG GGTAGATTTT GGACAAATCT GTACTCTTTG ACAGTTCCCT TTGGACAGAA ACCAAACATA GATGTTACTG ATGCAATGGT GGACCAGGCC TGGGATGCAC AGAGAATATT CAAGGAGGCC GAGAAGTTCT TTGTATCTGT TGGTCTTCCT AATATGACTC AAGGATTCTG GGAAAATTCC ATGCTAACGG ACCCAGGAAA TGTTCAGAAA GCAGTCTGCC ATCCCACAGC TTGGGACCTG GGGAAGGGCG ACTTCAGGAT CCTTATGTGC ACAAAGGTGA CAATGGACGA CTTCCTGACA GCTCATCATG AGATGGGGCA TATCCAGTAT GATATGGCAT ATGCTGCACA ACCTTTTCTG CTAAGAAATG GAGCTAATGA AGGATTCCAT GAAGCTGTTG GGGAAATCAT GTCACTTTCT GCAGCCACAC CTAAGCATTT AAAATCCATT GGTCTTCTGT CACCCGATTT TCAAGAAGAC AATGAAACAG AAATAAACTT CCTGCTCAAA CAAGCACTCA CGATTGTTGG GACTCTGCCA TTTACTTACA TGTTAGAGAA GTGGAGGTGG ATGGTCTTTA AAGGGGAAAT TCCCAAAGAC CAGTGGATGA AAAAGTGGTG GGAGATGAAG CGAGAGATAG TTGGGGTGGT GGAACCTGTG CCCCATGATG AAACATACTG TGACCCCGCA TCTCTGTTCC ATGTTTCTAA TGATTACTCA TTCATTCGAT ATTACACAAG GACCCTTTAC CAATTCCAGT TTCAAGAAGC ACTTTGTCAA GCAGCTAAAC ATGAAGGCCC TCTGCACAAA TGTGACATCT CAAACTCTAC AGAAGCTGGA CAGAAACTGT TCAATATGCT GAGGCTTGGA AAATCAGAAC CCTGGACCCT AGCATTGGAA AATGTTGTAG GAGCAAAGAA CATGAATGTA AGGCCACTGC TCAACTACTT TGAGCCCTTA TTTACCTGGC TGAAAGACCA GAACAAGAAT TCTTTTGTGG GATGGAGTAC CGACTGGAGT CCATATGCAG ACCAAAGCAT CAAAGTGAGG ATAAGCCTAA AATCAGCTCT TGGAGATAAA GCATATGAAT GGAACGACAA TGAAATGTAC CTGTTCCGAT CATCTGTTGC ATATGCTATG AGGCAGTACT TTTTAAAAGT AAAAAATCAG ATGATTCTTT TTGGGGAGGA GGATGTGCGA GTGGCTAATT TGAAACCAAG AATCTCCTTT AATTTCTTTG TCACTGCACC TAAAAATGTG TCTGATATCA TTCCTAGAAC TGAAGTTGAA AAGGCCATCA GGATGTCCCG GAGCCGTATC AATGATGCTT TCCGTCTGAA TGACAACAGC CTAGAGTTTC TGGGGATACA GCCAACACTT GGACCTCCTA ACCAGCCCCC TGTTTCCATA TGGCTGATTG TTTTTGGAGT TGTGATGGGA GTGATAGTGG TTGGCATTGT CATCCTGATC TTCACTGGGA TCAGAGATCG GAAGAAGAAA AATAAAGCAA GAAGTGGAGA AAATCCTTAT GCCTCCATCG ATATTAGCAA AGGAGAAAAT AATCCAGGAT TCCAAAACAC TGATGATGTT CAGACCTCCT TTTAGTAGTC GACCTCGAGT CATGTAATTA GTTATGTCAC GCTTACATTC ACGCCCTCCC CCCACATCCG CTCTAACCGA AAAGGAAGGA GTTAGACAAC CTGAAGTCTA GGTCCCTATT TATTTTTTTA TAGTTATGTT AGTATTAAGA ACGTTATTTA TATTTCAAAT TTTTCTTTTT TTTCTGTACA GACGCGTGTA CGCATGTAAC ATTATACTGA AAACCTTGCT TGAGAAGGTT TTGGGACGCT CGAAGGCTTT AATTTGCGGC CGGTACCCAA TTCGCCCTAT AGTGAGTCGT ATTACGCGCG CTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGTTTA CAATTTCCTG ATGCGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATAGGC AAGTGCACAA ACAATACTTA AATAAATACT ACTCAGTAAT AACCTATTTC TTAGCATTTT TGACGAAATT TGCTATTTTG TTAGAGTCTT TTACACCATT TGTCTCCACA CCTCCGCTTA CATCAACACC AATAACGCCA TTTAATCTAA GCGCATCACC AACATTTTCT GGCGTCAGTC CACCAGCTAA CATAAAATGT AAGCTTTCGG GGCTCTCTTG CCTTCCAACC CAGTCAGAAA TCGAGTTCCA ATCCAAAAGT TCACCTGTCC CACCTGCTTC TGAATCAAAC AAGGGAATAA ACGAATGAGG TTTCTGTGAA GCTGCACTGA GTAGTATGTT GCAGTCTTTT GGAAATACGA GTCTTTTAAT AACTGGCAAA CCGAGGAACT CTTGGTATTC TTGCCACGAC TCATCTCCAT GCAGTTGGAC GATATCAATG CCGTAATCAT TGACCAGAGC CAAAACATCC TCCTTAGGTT GATTACGAAA CACGCCAACC AAGTATTTCG GAGTGCCTGA ACTATTTTTA TATGCTTTTA CAAGACTTGA AATTTTCCTT GCAATAACCG GGTCAATTGT TCTCTTTCTA TTGGGCACAC ATATAATACC CAGCAAGTCA GCATCGGAAT CTAGAGCACA TTCTGCGGCC TCTGTGCTCT GCAAGCCGCA AACTTTCACC AATGGACCAG AACTACCTGT GAAATTAATA ACAGACATAC TCCAAGCTGC CTTTGTGTGC TTAATCACGT ATACTCACGT GCTCAATAGT CACCAATGCC CTCCCTCTTG GCCCTCTCCT TTTCTTTTTT CGACCGAATT AATTCTTAAT CGGCAAAAAA AGAAAAGCTC CGGATCAAGA TTGTACGTAA GGTGACAAGC TATTTTTCAA TAAAGAATAT CTTCCACTAC TGCCATCTGG CGTCATAACT GCAAAGTACA CATATATTAC GATGCTGTCT ATTAAATGCT TCCTATATTA TATATATAGT AATGTCGTTT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAA TATGATCCAA TATCAAAGGA AATGATAGCA TTGAAGGATG AGACTAATCC AATTGAGGAG TGGCAGCATA TAGAACAGCT AAAGGGTAGT GCTGAAGGAA GCATACGATA CCCCGCATGG AATGGGATAA TATCACAGGA GGTACTAGAC TACCTTTCAT CCTACATAAA TAGACGCATA TAAGTACGCA TTTAAGCATA AACACGCACT ATGCCGTTCT TCTCATGTAT ATATATATAC AGGCAACACG CAGATATAGG TGCGACGTGA ACAGTGAGCT GTATGTGCGC AGCTCGCGTT GCATTTTCGG AAGCGCTCGT TTTCGGAAAC GCTTTGAAGT TCCTATTCCG AAGTTCCTAT TCTCTAGAAA GTATAGGAAC TTCAGAGCGC TTTTGAAAAC CAAAAGCGCT CTGAAGACGC ACTTTCAAAA AACCAAAAAC GCACCGGACT GTAACGAGCT ACTAAAATAT TGCGAATACC GCTTCCACAA ACATTGCTCA AAAGTATCTC TTTGCTATAT ATCTCTGTGC TATATCCCTA TATAACCTAC CCATCCACCT TTCGCTCCTT GAACTTGCAT CTAAACTCGA CCTCTACATT TTTTATGTTT ATCTCTAGTA TTACTCTTTA GACAAAAAAA TTGTAGTAAG AACTATTCAT AGAGTGAATC GAAAACAATA CGAAAATGTA AACATTTCCT ATACGTAGTA TATAGAGACA AAATAGAAGA AACCGTTCAT AATTTTCTGA CCAATGAAGA ATCATCAACG CTATCACTTT CTGTTCACAA AGTATGCGCA ATCCACATCG GTATAGAATA TAATCGGGGA TGCCTTTATC TTGAAAAAAT GCACCCGCAG CTTCGCTAGT AATCAGTAAA CGCGGGAAGT GGAGTCAGGC TTTTTTTATG GAAGAGAAAA TAGACACCAA AGTAGCCTTC TTCTAACCTT AACGGACCTA CAGTGCAAAA AGTTATCAAG AGACTGCATT ATAGAGCGCA CAAAGGAGAA AAAAAGTAAT CTAAGATGCT TTGTTAGAAA AATAGCGCTC TCGGGATGCA TTTTTGTAGA ACAAAAAAGA AGTATAGATT CTTTGTTGGT AAAATAGCGC TCTCGCGTTG CATTTCTGTT CTGTAAAAAT GCAGCTCAGA TTCTTTGTTT GAAAAATTAG CGCTCTCGCG TTGCATTTTT GTTTTACAAA AATGAAGCAC AGATTCTTCG TTGGTAAAAT AGCGCTTTCG CGTTGCATTT CTGTTCTGTA AAAATGCAGC TCAGATTCTT TGTTTGAAAA ATTAGCGCTC TCGCGTTGCA TTTTTGTTCT ACAAAATGAA GCACAGATGC TTCGTTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
