pLV3-CMV-MDC1(human-repeat-opt)-3×FLAG-Puro
$142.00
In stock
SKU
PL30452
NM_014641.3;NFBD1
100μg liquid plasmid(Instantly):$163
| name | pLV3-CMV-MDC1(human-repeat-opt)-3×FLAG-Puro | another name | apLV3-CMV-MDC1(human-repeat-opt)-3×FLAG-Puro |
| Insert Size (bp) | 6267bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | pLV3-CMV-MCS-3×FLAG-Puro | 5′ sequencing primer | MDC1-R1（high GC） |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT AGAGCTAGCG AATTCGCCAC CATGGAGGAC ACCCAGGCTA TTGACTGGGA TGTTGAAGAA GAGGAGGAGA CAGAGCAATC CAGTGAATCC TTGAGGTGTA ACGTGGAGCC AGTAGGGCGG CTACATATCT TTAGTGGTGC CCATGGACCA GAAAAAGATT TCCCACTACA CCTCGGGAAG AATGTGGTAG GCCGAATGCC TGACTGCTCT GTGGCCCTGC CCTTTCCATC TATCTCCAAA CAACATGCAG AGATTGAAAT CTTAGCCTGG GACAAGGCAC CTATCCTCCG AGACTGTGGG AGCCTTAATG GTACTCAAAT CCTGAGACCT CCTAAGGTTT TGAGCCCTGG GGTGAGTCAC CGTCTGAGGG ACCAGGAATT GATTCTCTTT GCTGACTTGC TCTGCCAGTA CCATCGCCTG GATGTCTCTC TGCCCTTTGT CTCCCGGGGC CCTCTGACAG TAGAAGAGAC ACCCAGAGTA CAGGGAGAAA CTCAACCCCA GAGGCTTCTG TTGGCTGAGG ACTCGGAGGA GGAAGTAGAT TTTCTTTCTG AAAGGCGTAT GGTAAAAAAA TCAAGGACCA CATCTTCCTC TGTGATAGTT CCAGAGAGTG ATGAAGAGGG GCATTCCCCG GTCCTGGGCG GCCTTGGGCC GCCTTTTGCC TTCAATTTGA ACAGTGACAC AGATGTGGAA GAAGGTCAGC AACCAGCCAC AGAGGAGGCC TCCTCAGCTG CCAGAAGAGG TGCCACTGTA GAGGCAAAGC AGTCTGAAGC TGAAGTTGTA ACTGAAATCC AGCTTGAAAA GGATCAGCCT TTAGTGAAGG AGAGGGACAA TGATACAAAA GTCAAGAGGG GTGCAGGGAA TGGGGTGGTT CCAGCTGGGG TGATTCTGGA GAGGAGCCAA CCTCCTGGAG AGGACAGTGA CACAGATGTG GATGATGACA GCAGGCCTCC TGGAAGGCCA GCTGAGGTCC ATTTGGAAAG GGCTCAGCCT TTTGGCTTCA TCGACAGCGA CACTGATGCG GAAGAAGAGA GGATCCCAGC AACCCCAGTT GTCATTCCTA TGAAGAAGAG GAAGATCTTC CATGGAGTAG GTACAAGGGG TCCTGGAGCA CCAGGCCTGG CCCATCTGCA GGAGAGCCAG GCTGGTAGTG ATACAGATGT GGAAGAAGGC AAGGCCCCAC AGGCTGTCCC TCTGGAGAAA AGCCAAGCTT CCATGGTTAT CAACAGCGAT ACAGATGACG AGGAAGAAGT CTCAGCAGCG CTGACTTTGG CACATCTGAA AGAGAGCCAG CCTGCTATAT GGAACAGAGA TGCAGAAGAG GACATGCCCC AACGTGTGGT CCTTCTGCAG CGAAGCCAAA CCACCACTGA GAGAGACAGT GACACAGACG TGGAGGAGGA AGAGCTCCCA GTGGAAAATA GAGAAGCTGT CCTCAAGGAT CACACAAAGA TTAGAGCCCT TGTTAGAGCA CATTCAGAAA AGGACCAACC TCCTTTTGGG GACAGTGATG ACAGTGTGGA AGCAGATAAG AGCTCACCTG GGATCCACCT GGAGAGAAGC CAAGCCTCCA CCACAGTGGA CATCAACACA CAAGTGGAGA AGGAAGTCCC GCCAGGGTCA GCCATTATAC ATATAAAGAA GCATCAGGTG TCTGTGGAGG GGACAAATCA AACAGATGTG AAAGCAGTTG GGGGACCAGC AAAGCTGCTT GTGGTATCTC TAGAGGAAGC CTGGCCTCTG CATGGGGACT GTGAAACAGA TGCAGAGGAG GGCACCTCCC TAACAGCCTC AGTAGTTGCA GATGTAAGAA AGAGCCAGCT TCCAGCAGAA GGGGATGCTG GGGCAGAGTG GGCTGCAGCT GTTCTTAAGC AGGAGAGAGC TCATGAGGTG GGGGCCCAGG GTGGGCCACC TGTGGCACAA GTGGAGCAGG ACCTCCCTAT CTCAAGAGAG AACCTCACAG ATCTGGTGGT GGACACAGAC ACTCTAGGGG AATCCACCCA GCCACAGAGA GAGGGAGCCC AGGTCCCCAC AGGAAGGGAG AGAGAACAAC ATGTGGGTGG GACCAAGGAC TCTGAAGACA ACTATGGTGA TTCTGAAGAT CTGGACCTAC AAGCTACCCA GTGCTTTCTG GAGAATCAGG GCCTGGAAGC AGTCCAGAGC ATGGAGGATG AACCTACCCA GGCCTTCATG TTGACTCCAC CCCAAGAGCT TGGCCCTTCC CATTGCAGCT TCCAGACAAC AGGTACCCTA GATGAACCAT GGGAGGTCCT GGCTACACAG CCATTCTGTC TGAGAGAGTC TGAGGACTCT GAGACCCAGC CTTTTGACAC GCACCTTGAG GCCTATGGAC CTTGCCTGTC TCCACCTAGG GCAATACCAG GAGACCAACA TCCAGAGAGC CCAGTTCACA CAGAGCCAAT GGGGATTCAA GGCAGAGGGA GGCAGACTGT GGATAAAGTC ATGGGTATAC CAAAAGAAAC AGCAGAGAGG GTGGGCCCTG AGAGAGGGCC ATTGGAGAGA GAAACTGAGA AACTGCTACC AGAAAGACAG ACAGATGTGA CAGGAGAGGA AGAATTAACC AAGGGGAAAC AGGACAGAGA ACAAAAACAG TTGTTAGCTA GAGACACCCA GAGACAAGAA TCTGACAAAA ATGGGGAAAG TGCAAGTCCT GAAAGAGATA GGGAGAGTTT GAAGGTAGAA ATTGAGACAT CTGAGGAAAT ACAAGAGAAA CAAGTACAGA AGCAGACCCT TCCAAGCAAA GCATTTGAGA GAGAAGTAGA GAGACCAGTA GCAAACAGAG AGTGCGATCC AGCCGAGTTA GAAGAGAAGG TGCCCAAAGT GATCCTGGAG AGAGATACAC AGAGAGGGGA GCCAGAGGGA GGGAGCCAGG ACCAGAAAGG GCAGGCCTCC AGCCCAACAC CAGAGCCTGG GGTGGGGGCG GGGGACCTTC CGGGACCTAC CTCAGCCCCC GTACCTTCTG GGAGCCAGTC AGGTGGAAGG GGATCCCCAG TGAGCCCCAG GAGGCATCAG AAAGGCCTCC TGAATTGCAA GATGCCACCT GCTGAGAAGG CTTCCAGGAT CAGAGCTGCT GAGAAGGTTT CCAGGGGCGA TCAGGAATCT CCAGATGCTT GTCTGCCTCC TACAGTCCCC GAAGCCCCTG CCCCCCCTCA AAAACCCCTG AACAGCCAAT CCCAGAAGCA CCTCGCCCCT CCTCCGCTGC TCTCACCCCT TCTTCCAAGC ATCAAGCCAA CCGTGCGAAA GACCCGGCAG GACGGTTCTC AGGAAGCACC TGAGGCACCC CTTTCCTCAG AACTTGAGCC CTTCCATCCG AAGCCAAAGA TTAGGACAAG GAAGTCCAGC CGGATGACTC CTTTTCCTGC CACAAGTGCC GCTCCAGAAC CCCACCCATC TACCAGCACC GCTCAGCCAG TGACCCCGAA GCCCACTTCA CAGGCCACCA GGAGCCGCAC CAACCGCTCA TCTGTCAAGA CACCTGAACC AGTCGTGCCA ACGGCCCCAG AGCTCCAGCC CTCTACGTCC ACTGACCAAC CTGTGACCTC TGAGCCCACT TCACAAGTGA CTCGCGGTAG AAAGAGTAGA TCATCAGTGA AAACACCAGA GACGGTGGTG CCTACAGCCC TGGAACTGCA GCCCAGTACT AGTACTGATA GGCCGGTAAC ATCTGAACCT ACTTCACAGG CGACAAGGGG CCGAAAGAAT CGGAGCAGCG TGAAGACTCC TGAGCCCGTC GTGCCCACAG CACCTGAACT GCAGCCTTCC ACCTCAACTG ATCAGCCTGT CACCAGTGAA CCAACCTACC AAGCCACAAG AGGACGCAAG AACCGGTCAA GTGTTAAAAC GCCCGAGCCC GTAGTGCCTA CTGCTCCAGA ACTCCGGCCC TCTACCTCAA CTGACAGGCC CGTAACCCCC AAACCAACTA GCCGGACAAC CCGGAGCCGG ACCAACATGA GTTCCGTGAA AACGCCCGAA ACCGTTGTTC CAACAGCACC CGAACTGCAG ATAAGCACGT CCACAGACCA GCCAGTGACT CCAAAGCCCA CATCTCGCAC GACGAGGTCT AGGACCAATA TGAGTTCCGT GAAAAACCCC GAGTCTACTG TGCCAATTGC ACCCGAACTG CCTCCTTCCA CATCTACGGA ACAGCCTGTT ACCCCTGAGC CCACAAGTAG AGCCACTAGA GGCAGGAAAA ACCGATCAAG CGGCAAGACT CCTGAAACCC TCGTTCCAAC CGCACCTAAG CTGGAACCGT CCACATCCAC TGATCAGCCT GTGACACCCG AGCCCACAAG CCAGGCTACA AGGGGAAGAA CCAATCGGAG CTCTGTTAAG ACTCCAGAGA CTGTCGTGCC TACCGCTCCA GAGCTGCAGC CCAGTACGAG CACTGATCAG CCCGTCACCC CCGAACCTAC CTCACAAGCT ACCCGGGGTA GGACAGACAG ATCCTCAGTG AAGACACCCG AGACCGTAGT CCCCACCGCT CCAGAACTGC AGGCATCCGC CTCAACAGAC CAGCCTGTCA CTAGCGAGCC TACCTCTAGA ACCACTCGCG GACGAAAAAA CCGGAGCAGT GTCAAGACTC CCGAGACCGT GGTCCCTGCT GCACCAGAAC TTCAGCCCAG CACGAGCACC GACCAGCCGG TGACCCCAGA ACCCACTTCT CGCGCCACAC GGGGGCGGAC TAATCGCAGT TCCGTCAAAA CTCCAGAGTC AATCGTTCCT ATCGCACCCG AGCTGCAGCC ATCAACATCT CGAAACCAAC TCGTTACTCC GGAGCCAACC AGTAGAGCAA CAAGGTGCCG GACAAACAGA AGCAGTGTAA AAACTCCCGA ACCCGTGGTA CCTACAGCCC CTGAACCACA TCCCACTACA TCTACAGACC AGCCAGTTAC TCCCAAACTG ACATCACGGG CTACTCGGCG AAAAACCAAC AGAAGCAGTG TGAAGACGCC CAAGCCCGTC GAGCCAGCTG CTTCTGACCT GGAACCGTTC ACTCCTACTG ACCAGAGCGT GACTCCTGAG GCTATTGCCC AGGGGGGGCA GTCCAAAACC CTGCGGTCTT CCACTGTGAG AGCAATGCCG GTGCCGACTA CGCCCGAATT CCAGAGTCCA GTGACCACCG ATCAGCCGAT CAGCCCGGAG CCCATCACTC AACCCTCTTG TATCAAGAGG CAGCGGGCCG CCGGGAATCC CGGAAGTCTG GCTGCTCCCA TTGACCACAA ACCATGCTCT GCGCCCTTGG AACCTAAGTC CCAAGCGAGT CGCAATCAGC GGTGGGGTGC AGTTAGGGCT GCTGAAAGCT TGACCGCTAT TCCTGAGCCA GCCTCACCCC AGTTGCTGGA GACCCCGATC CACGCTAGTC AAATCCAGAA GGTAGAACCG GCCGGAAGAA GCCGATTCAC GCCGGAACTG CAGCCCAAAG CTTCTCAGAG TAGAAAACGG AGCTTGGCTA CAATGGATTC TCCTCCCCAC CAAAAGCAGC CGCAAAGGGG AGAAGTCAGT CAGAAGACCG TGATCATAAA GGAAGAAGAA GAGGACACCG CCGAGAAGCC AGGGAAGGAG GAAGACGTAG TCACCCCAAA GCCGGGGAAG AGGAAAAGAG ACCAGGCAGA AGAGGAACCC AACCGCATCC CCTCCAGATC CCTTCGGAGG ACGAAACTCA ATCAGGAATC TACAGCGCCC AAGGTCCTCT TCACAGGCGT GGTTGATGCA AGGGGCGAGA GAGCGGTGCT TGCATTGGGG GGATCCCTTG CTGGGAGCGC TGCCGAGGCC TCTCACTTGG TGACAGATCG AATAAGACGA ACAGTTAAGT TCTTGTGTGC CCTGGGAAGA GGTATTCCTA TTCTGTCACT GGACTGGCTC CATCAAAGCA GGAAAGCTGG GTTTTTCCTT CCGCCCGACG AGTACGTAGT GACAGACCCG GAGCAGGAAA AGAACTTTGG GTTTTCACTG CAAGATGCTT TGTCTAGAGC TCGCGAACGC CGGCTCCTGG AGGGGTACGA GATCTATGTA ACTCCTGGCG TGCAGCCACC ACCTCCCCAG ATGGGCGAAA TAATATCTTG TTGCGGGGGT ACTTACCTCC CCTCTATGCC CAGGTCCTAC AAGCCACAGC GGGTGGTGAT CACCTGCCCC CAGGACTTTC CCCATTGCTC CATCCCTCTG CGCGTTGGAT TGCCACTGTT GAGCCCCGAG TTCCTGCTGA CTGGCGTTCT GAAACAGGAG GCCAAGCCTG AGGCCTTTGT ACTCTCCCCG CTCGAGATGA GCTCAACAGG ATCCGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGACT ACAAGGATGA CGATGACAAG TAGCGGCCGC GAAGGATCTG CGATCGCTCC GGTGCCCGTC AGTGGGCAGA GCGCACATCG CCCACAGTCC CCGAGAAGTT GGGGGGAGGG GTCGGCAATT GAACGGGTGC CTAGAGAAGG TGGCGCGGGG TAAACTGGGA AAGTGATGTC GTGTACTGGC TCCGCCTTTT TCCCGAGGGT GGGGGAGAAC CGTATATAAG TGCAGTAGTC GCCGTGAACG TTCTTTTTCG CAACGGGTTT GCCGCCAGAA CACAGCTGAA GCTTCGAGGG GCTCGCATCT CTCCTTCACG CGCCCGCCGC CCTACCTGAG GCCGCCATCC ACGCCGGTTG AGTCGCGTTC TGCCGCCTCC CGCCTGTGGT GCCTCCTGAA CTGCGTCCGC CGTCTAGGTA AGTTTAAAGC TCAGGTCGAG ACCGGGCCTT TGTCCGGCGC TCCCTTGGAG CCTACCTAGA CTCAGCCGGC TCTCCACGCT TTGCCTGACC CTGCTTGCTC AACTCTACGT CTTTGTTTCG TTTTCTGTTC TGCGCCGTTA CAGATCCAAG CTGTGACCGG CGCCTACGCT AGATGACCGA GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCCGGGCCG TACGCACCCT CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGACCCGG ACCGCCACAT CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GAGTCGACAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAATCATCG TCCTTTCCTT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCCTG GTACCTTTAA GACCAATGAC TTACAAGGCA GCTGTAGATC TTAGCCACTT TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT AATTCACTCC CAACGAAAAT AAGATCTGCT TTTTGCTTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTAGTA GTTCATGTCA TCTTATTATT CAGTATTTAT AACTTGCAAA GAAATGAATA TCAGAGAGTG AGAGGAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGCTC TAGCTATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGACTTTT GCAGAGACGG CCCAAATTCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGCCAA GCTG
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