pBiFC-CMV-SF3B3(human)-VC155
$99.00
In stock
SKU
PL30033
NM_012426.5;RSE1; SAP130; SF3b130; STAF130
100μg liquid plasmid(Instantly):$120
| name | pBiFC-CMV-SF3B3(human)-VC155 | another name | apBiFC-CMV-SF3B3(human)-VC155 |
| Insert Size (bp) | 3654bp | Plasmid host | Mammary cell |
| Plasmid uses | Bimolecular fluorescence | Fragment type | BIFC |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pBiFC-VC155 | 5′ sequencing primer | SV40 intron-F1 |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ATGTACCCAT ACGATGTTCC AGATTACGCT CTTATGGCCA TGGAGGCCCG AATTCATATG TTTCTGTACA ACTTAACCTT GCAGAGAGCC ACTGGCATCA GCTTTGCCAT TCATGGAAAC TTTTCTGGAA CCAAACAACA AGAAATTGTT GTTTCCCGTG GGAAGATCTT GGAGCTGCTT CGCCCAGACC CCAACACTGG CAAAGTACAT ACCCTACTCA CTGTGGAAGT ATTCGGTGTT ATCCGGTCAC TCATGGCCTT TAGGCTGACA GGTGGCACCA AAGACTACAT TGTAGTTGGC AGTGACTCTG GTCGAATTGT TATTTTGGAA TACCAGCCAT CTAAGAATAT GTTTGAGAAG ATTCACCAAG AAACCTTTGG CAAGAGTGGA TGCCGTCGCA TCGTTCCTGG CCAGTTCTTA GCTGTGGATC CCAAAGGGCG AGCCGTTATG ATTAGTGCCA TTGAGAAACA GAAATTGGTG TATATTTTGA ACAGAGATGC TGCAGCCCGA CTTACCATTT CATCTCCCCT GGAAGCCCAC AAAGCAAACA CTTTAGTGTA TCATGTAGTT GGAGTAGATG TCGGATTTGA AAATCCAATG TTTGCTTGTC TGGAAATGGA TTATGAGGAA GCAGACAATG ATCCAACAGG GGAAGCAGCA GCTAATACCC AGCAGACACT TACTTTCTAT GAGCTAGACC TTGGTTTAAA TCATGTGGTC CGAAAATACA GTGAACCTTT GGAGGAACAC GGCAACTTCC TTATTACAGT TCCAGGAGGG TCAGATGGTC CAAGTGGAGT ACTGATCTGC TCTGAAAACT ATATTACTTA CAAGAACTTT GGTGACCAGC CAGATATCCG CTGTCCAATT CCCAGGAGGC GGAATGACCT GGATGACCCT GAAAGAGGAA TGATTTTTGT CTGCTCTGCA ACCCATAAAA CCAAATCGAT GTTCTTCTTT TTGGCTCAAA CTGAGCAGGG AGATATCTTT AAGATCACTT TGGAGACAGA TGAAGATATG GTTACTGAGA TCCGGCTCAA ATATTTTGAT ACTGTACCCG TTGCTGCTGC CATGTGTGTG CTTAAAACAG GGTTCCTTTT TGTAGCATCA GAATTTGGAA ACCATTACTT ATATCAAATT GCACATCTTG GAGATGATGA TGAAGAACCT GAGTTTTCAT CAGCCATGCC TCTGGAAGAA GGAGACACAT TCTTTTTTCA GCCAAGACCA CTTAAAAACC TTGTGCTGGT TGATGAGTTG GACAGCCTCT CTCCCATTCT GTTTTGCCAG ATAGCTGATC TGGCCAATGA AGATACTCCA CAGTTGTATG TGGCCTGTGG TAGGGGACCC CGATCATCTC TGAGAGTCCT AAGACATGGA CTTGAGGTGT CAGAAATGGC TGTTTCTGAG CTACCTGGTA ACCCCAACGC TGTCTGGACA GTGCGTCGAC ACATTGAAGA TGAGTTTGAT GCCTACATCA TTGTGTCTTT CGTGAATGCC ACCCTAGTGT TGTCCATTGG AGAAACTGTA GAAGAAGTGA CTGACTCTGG GTTCCTGGGG ACCACCCCGA CCTTGTCCTG CTCCTTATTA GGAGATGATG CCTTGGTGCA GGTCTATCCA GATGGCATTC GGCACATACG AGCAGACAAG AGAGTCAATG AGTGGAAGAC CCCTGGAAAG AAAACAATTG TGAAGTGTGC AGTGAACCAG CGACAAGTGG TGATTGCCCT GACAGGAGGA GAGCTGGTCT ATTTCGAGAT GGATCCTTCA GGACAGCTGA ATGAGTACAC AGAACGGAAG GAGATGTCAG CAGATGTGGT GTGCATGAGT CTGGCCAATG TACCCCCTGG AGAGCAGCGG TCTCGCTTCC TGGCTGTGGG GCTTGTGGAC AACACTGTCA GAATCATCTC CCTGGATCCC TCAGACTGTT TGCAACCTCT AAGCATGCAG GCTCTCCCAG CCCAGCCTGA GTCCTTGTGT ATCGTGGAAA TGGGTGGGAC TGAGAAGCAG GATGAGCTGG GTGAGAGGGG CTCGATTGGC TTCCTATACC TGAATATTGG GCTACAGAAC GGTGTGCTGC TGAGGACTGT CTTGGACCCT GTCACTGGGG ATTTGTCTGA TACTCGCACT CGGTACCTGG GGTCCCGTCC TGTGAAGCTC TTCCGAGTCC GAATGCAAGG CCAGGAGGCA GTATTGGCCA TGTCAAGCCG CTCATGGTTG AGCTATTCTT ACCAATCTCG CTTCCATCTC ACCCCACTGT CTTACGAGAC ACTGGAATTT GCATCGGGTT TTGCCTCGGA ACAGTGTCCC GAGGGCATTG TGGCCATCTC CACCAACACC CTACGGATTT TGGCATTAGA GAAGCTCGGT GCTGTCTTCA ATCAAGTAGC CTTCCCACTG CAGTACACAC CCAGGAAATT TGTCATCCAC CCTGAGAGTA ACAACCTTAT TATCATTGAA ACGGACCACA ATGCCTACAC TGAGGCCACG AAAGCTCAGA GAAAGCAGCA GATGGCAGAG GAAATGGTGG AAGCAGCAGG GGAGGATGAG CGGGAGCTGG CCGCAGAGAT GGCAGCAGCA TTCCTCAATG AAAACCTCCC TGAATCCATC TTTGGAGCTC CCAAGGCTGG CAATGGGCAG TGGGCCTCTG TGATCCGAGT GATGAATCCC ATTCAAGGGA ACACACTGGA CCTTGTCCAG CTGGAACAGA ATGAGGCAGC TTTTAGTGTG GCTGTGTGCA GGTTTTCCAA CACTGGTGAA GACTGGTATG TGCTGGTGGG TGTGGCCAAG GACCTGATAC TAAACCCCCG ATCTGTGGCA GGGGGCTTCG TCTATACTTA CAAGCTTGTG AACAATGGGG AAAAACTGGA GTTTTTGCAC AAGACTCCTG TGGAAGAGGT CCCTGCTGCT ATTGCCCCAT TCCAGGGGAG GGTGTTGATT GGTGTGGGGA AGCTGTTGCG TGTCTATGAC CTGGGAAAGA AGAAGTTACT CCGAAAATGT GAGAATAAGC ATATTGCCAA TTATATCTCT GGGATCCAGA CTATCGGACA TAGGGTAATT GTATCTGATG TCCAAGAAAG TTTCATCTGG GTTCGCTACA AGCGTAATGA AAACCAGCTT ATCATCTTTG CTGATGATAC CTACCCCCGA TGGGTCACTA CAGCCAGCCT CCTGGACTAT GACACTGTGG CTGGGGCAGA CAAGTTTGGC AACATATGTG TGGTGAGGCT CCCACCTAAC ACCAATGATG AAGTAGATGA GGATCCTACA GGAAACAAAG CCCTGTGGGA CCGTGGCTTG CTCAATGGGG CCTCCCAGAA GGCAGAGGTG ATCATGAACT ACCATGTCGG GGAGACGGTG CTGTCCTTGC AGAAGACCAC GCTGATCCCT GGAGGCTCAG AATCACTTGT CTATACCACC TTGTCTGGAG GAATTGGCAT CCTTGTGCCA TTCACGTCCC ATGAGGACCA TGACTTCTTC CAGCATGTGG AAATGCACCT GCGGTCTGAA CATCCCCCTC TCTGTGGGCG GGACCACCTC AGCTTTCGCT CCTACTACTT CCCTGTGAAG AATGTGATTG ATGGAGACCT CTGTGAGCAG TTCAATTCCA TGGAACCCAA CAAACAAAAG AACGTCTCTG AAGAACTGGA CCGAACCCCA CCCGAAGTGT CCAAGAAACT CGAGGATATC CGGACCCGCT ACGCCTTCGG TACCCGTCCG GCGTGCAAAA TCCCGAACGA CCTGAAACAG AAAGTCATGA ACCACGACAA GCAGAAGAAC GGCATCAAGG CCAACTTCAA GATCCGCCAC AACATCGAGG ACGGCGGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCT ACCAGTCCAA ACTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTAA GCGGCCGCGG GGATCCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA GGTGTGGGAG GTTTTTTCGG ATCCTCTAGA GTCGATCTGC AGGCATGCTA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCAGCTGG CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC AGTGAGTTCG AGCTTGCATG CCTGCAGGTC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC ATGGTGATGC GGTTTTGGCA GTACATCAAT GGGCGTGGAT AGCGGTTTGA CTCACGGGGA TTTCCAAGTC TCCACCCCAT TGACGTCAAT GGGAGTTTGT TTTGGCACCA AAATCAACGG GACTTTCCAA AATGTCGTAA CAACTCCGCC CCATTGACGC AAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG CAGAGCTCGT TTAGTGAACC GTCAGATCGC CTGGAGACGC CATCCACGCT GTTTTGACCT CCATAGAAGA CACCGGGACC GATCCAGCCT CCGGACTCTA GAGGATCCGG TACTAGAGGA ACTGAAAAAC CAGAAAGTTA ACTGGTAAGT TTAGTCTTTT TGTCTTTTAT TTCAGGTCCC GGATCCGGTG GTGGTGCAAA TCAAAGAACT GCTCCTCAGT GGATGTTGCC TTTACTTCTA GGCCTGTACG GAAGTGTTAC TTCTGCTCTA AAAGCTGCGG AATTGTACCC GCGGGCCCAC C
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