NM_001359875.1; 2600010B19Rik; 3110050K21Rik;4930570G11Rik
100μg liquid plasmid(Instantly):$149
| name | pLV3-CMV-Zc3h13(mouse)-CopGFP-Puro | another name | apLV3-CMV-Zc3h13(mouse)-CopGFP-Puro |
| Insert Size (bp) | 5193bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | pLV3-CMV-MCS2-EF1a-CopGFP-Puro | 5′ sequencing primer | EF1a-R |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT TATCGATACT AGTATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTTT ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGATTC TAGAGCTAGC CTGATACGAA CTCGGAATTC GCCACCATGT CAAAAATTAG GAGAAAGGTC ACAGTGGAAA ACACCAAGAC CATATCTGAG AGTACATCCC GAAGACCTAG CGTATTTGAG AGGCTTGGCC CCAGCACTGG AAGCACAACA GAGACACAGT GTCGTAACTG GCTGAAGACT GGCAGCTGCC TCTATGGAAA CACATGTAGG TTCATACATG GCCCTTCACC TCGTGGGAAA GGCTACAGCA GCAATTACAG AAGGTCACCA GAAAGACCCA CAGGTGACCT CAGAGAAAGA ATGAAGAATA AGCGTCAGGA TGTGGATTCT GAATCCCAGA AACGGAATAC AGAGGAGCCA TCCTCACCTG TTAGGAAAGA ATCTTCAAGA GGGAGACATA GAGACAAGGA AGACATAAAA ATTGTTAAGG AGAGAACTCC AGAAAGTGAA GAAGAAAATG TAGAATGGGA AACTAACAGA GATGATTCTG ACAATGGAGA TATTAATTAT GATTATGTTC ATGAGTTATC ATTGGAAATG AAGCGGCAGA AAATACAGAG AGAATTGATG AAATTGGAAC AAGAAAATAT GGATAAGAGA GAAGAAATTA TCATTCAAAA GGAGGTTTCC CCAGAAGTGG TTAGATCAAA ATTGTCTCCA TCACCTTCTC TGAGAAAGTC CAGTAAATCT CCAAAACGAA AGTCCAGCCC CAAGGCATCT TCAGCTGGCA AGAAGGAGCG GAAGGCTGCT GTGGTGGCTT CTCCGCTGTT GGACCAGCAG AGAAATTCAA AGGGCAACCA AAGTAAAAAG AAAGGGCCGC GCACTCCGAG TCCACCTCCT CCTATACTAG AAGATATCAT TCTGGGGAAA AAGTACAAAG AAAAGTATAA AGTGAAAGAC AGGATAGAAG AAAAACCAAG AGATGGGAAG GACAGAGGCC GGGATTTTGA AAAGCAAAGA GAAAAACGAG ACAAGCCAAG GTCTTCTTCT CCGGGACAGC ACCATTCTCC TCTGTCTTCT AGACATCACT CGTCATCCTC ACAGTCAGGG TCATCTATTC AGAGACACTC TCCTTCTCCT CGCCGGAAGA GAACTCCTTC CCCATCCTAC CAGAGGACTT TAACTCCATC TTTACGACGT TCTGCCTCTC CTTATCCAAC CCACTGTCTG TCTTCTCCTC AGAGAAAGCA AAGTCCTCCA AGACATCGCT CTCCAATGCG AGAGAAAGGG AGGCATGATC ACGAACGGAC TTCACAGTCA CATGACCGGC GTCACGAAAG GAGGGAAGAA ACTCGAGGCA AAAGGGATAG AGAAAAGGAT ACCAGAGAAG AGCGAGAGTC TGAACATGAC CACAGGGATG ACAGAGAACC ACGAGACAGC CGGGACCGGA GAGACACTAG AGACCGCAGG GAACTTAGAG ACTCCAGAGA CATGCGGGAC TCCAGAGAGA TGAGGGATTA TAGCAGAGAT GCCAAGGAGA GCCGCGATCC CAGAGACTCC CGGTCAGCTC GGGATGTCCA TGACTACAGG GACCGTGAAG CTCGGGATGC TCATGCTCGA GATGTTCGAG ATGCTAGGGA TGCTCGGGAT GCTAGGGATG CTCGGGATAT TCGGGATGTC CGTGATGTCC GGGATGTCCG GGATGTCCGG GATGTCCGGG ATGTGCGAGA TGTGCGTGAT GTCCGAGATG TTCGAGATGC TCGCGATGTC CGGGATGTCC GGGATGCTCG CGATGTCCGG GATGTCCGGG ATGTTCGGGA TGGTCATCGA AAGGAGGATG TATACCAGGA AGAAGCCCGC AGTTATGGCA GAAACCACCT GAGAGAAGAG AGTTCTAGAG TTGAACTAAG GAATGACTCT AGAAATGAGT CTCGAAGTGA AATTAGAAAC GATCGGATGG GCAGAAGTAG GGGGAGAGGT CCAGAACTGC CTGAAAAGGG AAGTCGAGGA ACAAGAGGTT CCCAAATGGA TAGCCACAGT AGTGGCAGCA ACTACCATGA CAGCTGGGAA ACTCGCAGTA GCTATCCTGA AAGAGACAGA TACCCTGAGA GAGACACTCG GGATCCGGCC AGGGATTCTT CCTTTGAGAG AAGGCATGGA GAAAGAGACC GTCGGGATAA CAGGGAACGA GATCAAAGAC CAAGCTCACC TATTCGCCAT CAGGGAAGGA GTGAGGAGCT TGAGCGAGAT GAAAGAAGAG AGGAACGAAG AATAGACAGA GTGGATGAGA GACGAGACGA TAGGGTCAGG GACAGAGATA GGGATCGAGA GCGAGAAAGG GAGCGGGAGA GGGAACGAGA GCGGGAGCGA GAAAAGGAAA GAGAAAGAGA ACTAGAAAGA GAGAGAGCTA GAGAGCGGGA AAGAGAGAGA GAGAAAGAGA GGGAGAGGGA GCGAGAACGA GAGAGAGATC AGCGAGATCG TGACCATGAT CGAGAGCGAG AAAGAGAGAG GGAGCGAGAG AGGGAAAAAG AACGGGAGCG AGAGCGAGAA GAGAGAGAGA GGGAGAGAGA AAGGGAAAGG GAACGGGAGA GAGAGAGGGA ACGGGAGCGG GAGAGAGAGC GAGAACGGGA GCGGGAGCGA GAGAGGGCCA GAGAAAGAGA AAAAGAACGA GAACGGCAAA GAGAGTGGGA AGATAAAGAC AAGGGACGCG ATGACCGGAG GGAGAAGCGC GAGGATATTC ATGTTCGAGA GGACAGGATC CCCAGAGACA GCCACGAGGA GAGGAAGTCC AAGAAACGCT ATAGAAATGA AGGGAGTCCC AGCCCCCGGC AGTCCCCCAA GCGCCGGCGG GAGCATTCTC CTGACAGTGA CACCTACCAC AGTGGGGATG ACAAAAATGA AAAACATAGA CTCTTGAGCC AGGTTGTACG GCCACAGGAA TCTCGTTCTC TCAGCCCATC CCACCTCACA GAAGATAGGC AAGGTAGATG GAAAGAAGAG GATCGTAAAT CAGAAAGAAA GGAGAGTTCC AGGCGCTACG AAGAGCAAGA ACTCAAAGAG AAGCTTTCTT GTGGCGACAG GCAAAGGGAG CAGGCCGAAA GCGTGGACAG CTCGAGAGTG CGCGCCCAAG ACCTCCTCAG CCATCGCCAG GCTGAAGACC GCGACCGTGA CGGGTCTGAT CGAGCCCATG ATGAAAAGAA AAAAGCAAAA GCTCCGAAGA AACCTGTAAA GAAAAAGAAG GAGGAGGATG TTGGAGTAGA GAGGGGCAAC CTTGAGACAC ATGAGGATAG TCAGGTATTT TCACCAAAAA AAGGACAGAA GAAGAAAAAC ATTGAAAAAA AGCGTAAAAG ATCCAAGGGT GATTCTGATG TTTCTGATGA GGAGGCAGCC CCGCAGAATA AGAAGAAGAG AGGGCCTCGC ACACCCCCTC TGGCCATCAA GGAGGAGCTG GCTGACATCT CCACTGACAA GGATGGCGTC CTGGAGGATC CACTGAAGAA AGAAAATACA GCCTTTAGTG ACTGGTCTGA TGAGGATGTG CCTGACCGCA CAGAGGGGCT GGAAGCCGAG CACACCGCAG CCACTGCCAC TCCTGGTAGC ACCCCTTCCC CCCTGTCTTC TCTTCTCCCT CCTCCACCCC CTGTGGCTGC TGCCAGTACT GCAGCCACTG CTCTTGCCTC CTCAGCTGTT TCTGCTACCA CCTCTGCCAC CTCTTCAAGC TCTGCTGCCA CCTCCAATAC CAATGGCAGT GAAGACTCAC ATAGAAAGTG CCACAGGGCA CGAGGGGAGA AGGTAGAGGT GTCCCATGTG ACCCTAGAAG ACACACCACA TCGCAAGCTG GTGGATCAAA AAAGAAGCAG CAGCCTCGGA AGCAATCGAA GTCACCGCAG CCACACCTCT GGTCGCCTAC GCTCCCCATC TAATGATTCT GCTCATCGAA GTGGGGATGA CCAGGGTAGT CGGAAGAGAG TGCTCCATAG TGGCTCCAGG GATAGAGAGA AAACAAAAAG CCTAGAAATC ACAGGAGAAA GAAAATCTAG GATTGATCAG TTAAAGCGTG GAGAACCCAG TCGAAGTACT TCTTCAGATC GCCAGGATTC CAGAAGCCAT AGCTCAAGAA GAAGCTCTCC TGAGTCGGAT CGGCAGGTTC ACTCAAGGTC CGGGTCGTTT GACAGTAGAG ACCGACTTCA AGAACGAGAT AGGTATGAGC ATGACAGAGA GCGGGAGAGG GACCGGAGAG ATCCCAGACA GCGGGAATGG GATCGTGAAG CGGAGAAAGA GTGGCCTCGG ACCAGAGACC GAGACAGGCT TCGGGAACGA GACAGAGACC GGGACAGAAG GAGAGACTTG GACAGAGAAA GAGAGAGACT GATCTCTGAC CCCATGGAAA GGGACAGAGA ACGAGAGAGA ACTTTTGAGA CATCTCAGCT GGAATCTGGG AAACGAAGCG AAGTCAAATT AGAGAGTGAA CATGAGAGAG ATCTGGAAGG CAGCTCCCGG GACTCCGTGG CCTTGGACAA AGAAAGGATG GACAGAGACC TGGGCTCTGT GCAGGGATTT GAAGATGTGA GTAAAGCTGA GAGAACAGAG AGTCTTGAAG CAGGAGATGA TGAATCCAAG TTAGATGATG CACATTCATT AGGATCTGGT GCTGGGGAAG GATATGAGCC CATCAGTGAT GACGAGCTAG ATGAAATTCT GGCAGGGGAT GCAGAAAAGA GAGAGGACCA GCAGGAAGAA GAGAAGATGC CAGATCCATT AGATGTAATA GATGTGGACT GGTCTGGACT AATGCCAAAG CACCCGAAAG AACCGAGAGA GCCTGGGGCT GCACTCTTAA AGTTCACGCC TGGAGCTGTT CTGCTACGAG TTGGGATTTC TAAAAAGTTG GCTGGTTCTG AGCTCTTTAC CAAAGTTAAA GAAACATGTC AACAACTTGT AGAAAAACCC AAAGATGCAG ACAGCCTTTT CGAACATGAA CTAGGGGCTC TCAACATGGC TGCTTTACTT CGAAAAGAAG AGCGAGCAAG TCTTCTTAGT GACCTGGGAC CATGTTGTAA AGCATTATGT TTCAGACGGG ACTCTGCAAT CCGGAAGCAG CTTGTGAAGA ACGAAAAGGG AACTGTGAAG CAGGCGTACA CAAACACTCC GATGGTAGAC AATGAGCTGC TTCGGCTAAG TCTTCGGTTA TTTAAGAAGA AGGCCACCTG CCATGCCCCT GGACAGGAAA AGACTGAAGA TGGCAAATTA GGACCGTGCA GTATCCAGCA GGAGCTGTGT GTGTCCTAGG GATCCCTGCG ATATCCAGCG CGGCCGCGAA GGATCTGCGA TCGCTCCGGT GCCCGTCAGT GGGCAGAGCG CACATCGCCC ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC GGCAATTGAA CGGGTGCCTA GAGAAGGTGG CGCGGGGTAA ACTGGGAAAG TGATGTCGTG TACTGGCTCC GCCTTTTTCC CGAGGGTGGG GGAGAACCGT ATATAAGTGC AGTAGTCGCC GTGAACGTTC TTTTTCGCAA CGGGTTTGCC GCCAGAACAC AGCTGAAGCT TCGAGGGGCT CGCATCTCTC CTTCACGCGC CCGCCGCCCT ACCTGAGGCC GCCATCCACG CCGGTTGAGT CGCGTTCTGC CGCCTCCCGC CTGTGGTGCC TCCTGAACTG CGTCCGCCGT CTAGGTAAGT TTAAAGCTCA GGTCGAGACC GGGCCTTTGT CCGGCGCTCC CTTGGAGCCT ACCTAGACTC AGCCGGCTCT CCACGCTTTG CCTGACCCTG CTTGCTCAAC TCTACGTCTT TGTTTCGTTT TCTGTTCTGC GCCGTTACAG ATCCAAGCTG TGACCGGCGC CTACGCTAGA CGCCACCATG GAGAGCGACG AGAGCGGCCT GCCCGCCATG GAGATCGAGT GCCGCATCAC CGGCACCCTG AACGGCGTGG AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA CCCCCAAGCA GGGCCGCATG ACCAACAAGA TGAAGAGCAC CAAAGGCGCC CTGACCTTCA GCCCCTACCT GCTGAGCCAC GTGATGGGCT ACGGCTTCTA CCACTTCGGC ACCTACCCCA GCGGCTACGA GAACCCCTTC CTGCACGCCA TCAACAACGG CGGCTACACC AACACCCGCA TCGAGAAGTA CGAGGACGGC GGCGTGCTGC ACGTGAGCTT CAGCTACCGC TACGAGGCCG GCCGCGTGAT CGGCGACTTC AAGGTGGTGG GCACCGGCTT CCCCGAGGAC AGCGTGATCT TCACCGACAA GATCATCCGC AGCAACGCCA CCGTGGAGCA CCTGCACCCC ATGGGCGATA ACGTGCTGGT GGGCAGCTTC GCCCGCACCT TCAGCCTGCG CGACGGCGGC TACTACAGCT TCGTGGTGGA CAGCCACATG CACTTCAAGA GCGCCATCCA CCCCAGCATC CTGCAGAACG GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG AGGAGCTGCA CAGCAACACC GAGCTGGGCA TCGTGGAGTA CCAGCACGCC TTCAAGACCC CCATCGCCTT CGCCAGATCC CGCGCTCAGT CGTCCAATTC TGCCGTGGAC GGCACCGCCG GACCCGGCTC CACCGGATCT CGCGAGGGCA GAGGAAGTCT TCTAACATGC GGTGACGTGG AGGAGAATCC CGGCCCTATG ACCGAGTACA AGCCCACGGT GCGCCTCGCC ACCCGCGACG ACGTCCCCAG GGCCGTACGC ACCCTCGCCG CCGCGTTCGC CGACTACCCC GCCACGCGCC ACACCGTCGA TCCGGACCGC CACATCGAGC GGGTCACCGA GCTGCAAGAA CTCTTCCTCA CGCGCGTCGG GCTCGACATC GGCAAGGTGT GGGTCGCGGA CGACGGCGCC GCGGTGGCGG TCTGGACCAC GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT CGCCGAGATC GGCCCGCGCA TGGCCGAGTT GAGCGGTTCC CGGCTGGCCG CGCAGCAACA GATGGAAGGC CTCCTGGCGC CGCACCGGCC CAAGGAGCCC GCGTGGTTCC TGGCCACCGT CGGCGTCTCG CCCGACCACC AGGGCAAGGG TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT GGAGGCGGCC GAGCGCGCCG GGGTGCCCGC CTTCCTGGAG ACCTCCGCGC CCCGCAACCT CCCCTTCTAC GAGCGGCTCG GCTTCACCGT CACCGCCGAC GTCGAGGTGC CCGAAGGACC GCGCACCTGG TGCATGACCC GCAAGCCCGG TGCCTGAAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAGCTGACGT CCTTTCCATG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTCCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT TGGCCGCCTC CCCGCCTGGT ACCTTTAAGA CCAATGACTT ACAAGGCAGC TGTAGATCTT AGCCACTTTT TAAAAGAAAA GGGGGGACTG GAAGGGCTAA TTCACTCCCA ACGAAAATAA GATCTGCTTT TTGCTTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG TCAGTGTGGA AAATCTCTAG CAGTAGTAGT TCATGTCATC TTATTATTCA GTATTTATAA CTTGCAAAGA AATGAATATC AGAGAGTGAG AGGAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGCTCTA GCTATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGACTTTTGC AGAGACGGCC CAAATTCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCAGCTGG CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC AGTGCCAAGC TG
