pLV3-CMV-CAD(human)-Fluc-Puro
$142.00
In stock
SKU
PL29929
NM_004341.5,CDG1Z/DEE50/EIEE50/GATD4
100μg liquid plasmid(Instantly):$163
| name | pLV3-CMV-CAD(human)-Fluc-Puro | another name | apLV3-CMV-CAD(human)-Fluc-Puro |
| Insert Size (bp) | 6678bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Fluc |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | pLV3-CMV-MCS-Fluc-Puro | 5′ sequencing primer | CMV-F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTTAACTTT TAAAAGAAAA GGGGGGATTG GGGGGTACAG TGCAGGGGAA AGAATAGTAG ACATAATAGC AACAGACATA CAAACTAAAG AATTACAAAA ACAAATTACA AAAATTCAAA ATTTTATCGA TACTAGTATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG CCATCCACGC TGTTTTGACC TCCATAGAAG ATTCTAGAGC TAGCGAATTC GCCACCATGG CGGCCCTAGT GTTGGAGGAC GGGTCGGTCC TGCGGGGCCA GCCCTTTGGG GCCGCCGTGT CGACTGCCGG GGAAGTGGTG TTTCAAACCG GCATGGTCGG CTACCCCGAG GCCCTCACTG ATCCCTCCTA CAAGGCACAG ATCTTAGTGC TCACCTATCC TCTGATCGGC AACTATGGCA TCCCCCCAGA TGAAATGGAT GAGTTCGGTC TCTGCAAGTG GTTTGAATCC TCGGGCATCC ACGTAGCAGC ACTGGTAGTG GGAGAGTGCT GTCCTACTCC CAGCCACTGG AGTGCCACCC GCACCCTGCA TGAGTGGCTG CAGCAGCATG GCATCCCTGG CTTGCAAGGA GTAGACACTC GGGAGCTGAC CAAGAAGTTG CGGGAACAGG GGTCTCTGCT GGGGAAGCTG GTCCAGAATG GAACAGAACC TTCATCCCTG CCATTCTTGG ACCCCAATGC CCGCCCCCTG GTACCAGAGG TCTCCATTAA GACTCCACGG GTATTCAATA CAGGGGGTGC CCCTCGGATC CTTGCTTTGG ACTGTGGCCT CAAGTATAAT CAGATCCGAT GCCTCTGCCA GCGTGGGGCT GAGGTCACTG TGGTACCCTG GGACCATGCA CTAGACAGCC AAGAGTATGA GGGTCTCTTC TTAAGTAATG GGCCTGGTGA CCCTGCCTCC TATCCCAGTG TCGTATCCAC ACTGAGCCGT GTTTTATCTG AGCCTAATCC CCGACCTGTC TTTGGGATCT GCCTGGGACA CCAGCTATTG GCCTTAGCCA TTGGGGCCAA GACTTACAAG ATGAGATATG GGAACCGAGG CCATAACCAG CCCTGCTTGT TGGTGGGCTC TGGGCGCTGC TTTCTGACAT CCCAGAACCA TGGGTTTGCT GTGGAGACAG ACTCACTGCC AGCAGACTGG GCTCCTCTCT TCACCAACGC CAATGATGGT TCCAATGAAG GCATTGTGCA CAACAGCTTG CCTTTCTTCA GTGTCCAGTT TCACCCAGAG CACCAAGCTG GCCCTTCAGA TATGGAACTG CTTTTCGATA TCTTTCTGGA AACTGTGAAA GAGGCCACAG CTGGGAACCC TGGGGGCCAG ACAGTTAGAG AGCGGCTGAC TGAGCGCCTC TGTCCCCCTG GGATTCCCAC TCCCGGCTCT GGACTTCCAC CACCACGAAA GGTTCTGATC CTGGGCTCAG GGGGCCTCTC CATTGGCCAA GCTGGAGAAT TTGACTACTC GGGCTCTCAG GCAATTAAGG CCCTGAAGGA GGAAAACATC CAGACGTTGC TGATCAACCC CAATATTGCC ACAGTGCAGA CCTCCCAGGG GCTGGCCGAC AAGGTCTATT TTCTTCCCAT AACACCTCAT TATGTAACCC AGGTGATACG TAATGAACGC CCCGATGGTG TGTTACTGAC TTTTGGGGGC CAGACTGCTC TGAACTGTGG TGTGGAGCTG ACCAAGGCCG GGGTGCTGGC TCGGTATGGG GTCCGGGTCC TGGGCACACC AGTGGAGACC ATTGAGCTGA CCGAGGATCG ACGGGCCTTT GCTGCCAGAA TGGCAGAGAT CGGAGAGCAT GTGGCCCCGA GCGAGGCAGC AAATTCTCTT GAACAGGCCC AGGCAGCCGC TGAACGGCTG GGGTACCCTG TGCTAGTGCG TGCAGCCTTT GCCCTGGGTG GCCTGGGCTC TGGCTTTGCC TCTAACAGGG AGGAGCTCTC TGCTCTCGTG GCCCCAGCTT TTGCCCATAC CAGCCAAGTG CTAGTAGACA AGTCTCTGAA GGGATGGAAG GAGATTGAGT ACGAGGTGGT GAGAGACGCC TATGGCAACT GTGTCACGGT GTGTAACATG GAGAACTTGG ACCCACTGGG CATCCACACT GGTGAGTCCA TAGTGGTGGC CCCTAGCCAG ACACTGAATG ACAGGGAGTA TCAGCTCCTG AGGCAGACAG CTATCAAGGT GACCCAGCAC CTGGGAATTG TTGGGGAGTG CAATGTGCAG TATGCCTTGA ACCCTGAGTC TGAGCAGTAT TACATCATTG AAGTGAATGC CAGGCTCTCT CGCAGCTCTG CCCTGGCCAG TAAGGCCACA GGTTATCCAC TGGCTTATGT GGCAGCCAAG CTAGCATTGG GCATCCCTTT GCCTGAGCTC AGGAACTCTG TGACAGGGGG TACAGCAGCC TTTGAACCCA GCGTGGATTA TTGTGTGGTG AAGATTCCTC GATGGGACCT TAGCAAGTTC CTGCGAGTCA GCACAAAGAT TGGGAGCTGC ATGAAGAGCG TTGGTGAAGT CATGGGCATT GGGCGTTCAT TTGAGGAGGC CTTCCAGAAG GCCCTGCGCA TGGTGGATGA GAACTGTGTG GGCTTTGATC ACACAGTGAA ACCAGTCAGC GATATGGAGT TGGAGACTCC AACAGATAAG CGGATTTTTG TGGTGGCAGC TGCTTTGTGG GCTGGTTATT CAGTGGACCG CCTGTATGAG CTCACACGCA TCGACCGCTG GTTCCTGCAC CGAATGAAGC GTATCATCGC ACATGCCCAG CTGCTAGAAC AACACCGTGG ACAGCCTTTG CCGCCAGACC TGCTGCAACA GGCCAAGTGT CTTGGCTTCT CAGACAAACA GATTGCCCTT GCAGTTCTGA GCACAGAGCT GGCTGTTCGC AAGCTGCGTC AGGAACTGGG GATCTGTCCA GCAGTGAAAC AGATTGACAC AGTTGCAGCT GAGTGGCCAG CCCAGACAAA TTACCTATAC CTAACGTATT GGGGCACCAC CCATGACCTC ACCTTTCGAA CACCTCATGT CCTAGTCCTT GGCTCTGGCG TCTACCGTAT TGGCTCTAGC GTTGAATTTG ACTGGTGTGC TGTAGGCTGC ATCCAGCAGC TCCGAAAGAT GGGATATAAG ACCATCATGG TGAACTATAA CCCAGAGACA GTCAGCACCG ACTATGACAT GTGTGATCGA CTCTACTTTG ATGAGATCTC TTTTGAGGTG GTGATGGACA TCTATGAGCT CGAGAACCCT GAAGGTGTGA TCCTATCCAT GGGTGGACAG CTGCCCAACA ACATGGCCAT GGCGTTGCAT CGGCAGCAGT GCCGGGTGCT GGGCACCTCC CCTGAAGCCA TTGACTCGGC TGAGAACCGT TTCAAGTTTT CCCGGCTCCT TGACACCATT GGTATCAGCC AGCCTCAGTG GAGGGAGCTC AGTGACCTCG AGTCTGCTCG CCAATTCTGC CAGACCGTGG GGTACCCCTG TGTGGTGCGC CCCTCCTATG TGCTGAGCGG TGCTGCTATG AATGTGGCCT ACACGGATGG AGACCTGGAG CGCTTCCTGA GCAGCGCAGC AGCCGTCTCC AAAGAGCATC CCGTGGTCAT CTCCAAGTTC ATCCAGGAGG CTAAGGAGAT TGACGTGGAT GCCGTGGCCT CTGATGGTGT GGTGGCAGCC ATCGCCATCT CTGAGCATGT GGAGAATGCA GGTGTGCATT CAGGTGATGC GACGCTGGTG ACCCCCCCAC AAGATATCAC TGCCAAAACC CTGGAGCGGA TCAAAGCCAT TGTGCATGCT GTGGGCCAGG AGCTACAGGT CACAGGACCC TTCAATCTGC AGCTCATTGC CAAGGATGAC CAGCTGAAAG TTATTGAATG CAACGTACGT GTCTCTCGCT CCTTCCCCTT CGTTTCCAAG ACACTGGGTG TGGACCTAGT AGCCTTGGCC ACGCGGGTCA TCATGGGGGA AGAAGTGGAA CCTGTGGGGC TAATGACTGG TTCTGGAGTC GTGGGAGTAA AGGTGCCTCA GTTCTCCTTC TCCCGCTTGG CGGGTGCTGA CGTGGTGTTG GGTGTGGAAA TGACCAGTAC TGGGGAGGTG GCCGGCTTTG GGGAGAGCCG CTGTGAGGCA TACCTCAAGG CCATGCTAAG CACTGGCTTT AAGATCCCCA AGAAGAATAT CCTGCTGACC ATTGGCAGCT ATAAGAACAA AAGCGAGCTG CTCCCAACTG TGCGGCTACT GGAGAGCCTG GGCTACAGCC TCTATGCCAG TCTCGGCACA GCTGACTTCT ACACTGAGCA TGGCGTCAAG GTAACAGCTG TGGACTGGCA CTTTGAGGAG GCTGTGGATG GTGAGTGCCC ACCACAGCGG AGCATCCTGG AGCAGCTAGC TGAGAAAAAC TTTGAGCTGG TGATTAACCT GTCAATGCGT GGAGCTGGGG GCCGGCGTCT CTCTTCCTTT GTCACCAAGG GCTACCGCAC CCGACGCTTG GCCGCTGACT TCTCCGTGCC CCTAATCATC GATATCAAGT GCACCAAACT CTTTGTGGAG GCCCTAGGCC AGATCGGGCC AGCCCCTCCT TTGAAGGTGC ATGTTGACTG TATGACCTCC CAAAAGCTTG TGCGACTGCC GGGATTGATT GATGTCCATG TGCACCTGCG GGAACCAGGT GGGACACATA AGGAGGACTT TGCTTCAGGC ACAGCCGCTG CCCTGGCTGG GGGTATCACC ATGGTGTGTG CCATGCCTAA TACCCGGCCC CCCATCATTG ACGCCCCTGC TCTGGCCCTG GCCCAGAAGC TGGCAGAGGC TGGCGCCCGG TGCGACTTTG CGCTATTCCT TGGGGCCTCG TCTGAAAATG CAGGAACCTT GGGCACCGTG GCCGGGTCTG CAGCCGGGCT GAAGCTTTAC CTCAATGAGA CCTTCTCTGA GCTGCGGCTG GACAGCGTGG TCCAGTGGAT GGAGCATTTC GAGACATGGC CCTCCCACCT CCCCATTGTG GCTCACGCAG AGCAGCAAAC CGTGGCTGCT GTCCTCATGG TGGCTCAGCT CACTCAGCGC TCAGTGCACA TATGTCACGT GGCACGGAAG GAGGAGATCC TGCTAATTAA AGCTGCAAAG GCACGGGGCT TGCCAGTGAC CTGCGAGGTG GCTCCCCACC ACCTGTTCCT AAGCCATGAT GACCTGGAGC GCCTGGGGCC TGGGAAGGGG GAGGTCCGGC CTGAGCTTGG CTCCCGCCAG GATGTGGAAG CCCTGTGGGA GAACATGGCT GTCATCGACT GCTTTGCCTC AGACCATGCT CCCCATACCT TGGAGGAGAA GTGTGGGTCC AGGCCCCCAC CTGGGTTCCC AGGGTTAGAG ACCATGCTGC CACTACTCCT GACGGCTGTA AGCGAGGGCC GGCTCAGCCT GGACGACCTG CTGCAGCGAT TGCACCACAA TCCTCGGCGC ATCTTTCACC TGCCCCCGCA GGAGGACACC TATGTGGAGG TGGATCTGGA GCATGAGTGG ACAATTCCCA GCCACATGCC CTTCTCCAAG GCCCACTGGA CACCTTTTGA AGGGCAGAAA GTGAAGGGCA CCGTCCGCCG TGTGGTCCTG CGAGGGGAGG TTGCCTATAT CGATGGGCAG GTTCTGGTAC CCCCGGGCTA TGGACAGGAT GTACGGAAGT GGCCACAGGG GGCTGTTCCT CAGCTCCCAC CCTCAGCCCC TGCCACTAGT GAGATGACCA CGACACCTGA AAGACCCCGC CGTGGCATCC CAGGGCTTCC TGATGGCCGC TTCCATCTGC CGCCCCGAAT CCATCGAGCC TCCGACCCAG GTTTGCCAGC TGAGGAGCCA AAGGAGAAGT CCTCTCGGAA GGTAGCCGAG CCAGAGCTGA TGGGAACCCC TGATGGCACC TGCTACCCTC CACCACCAGT ACCGAGACAG GCATCTCCCC AGAACCTGGG GACCCCTGGC TTGCTGCACC CCCAGACCTC ACCCCTGCTG CACTCATTAG TGGGCCAACA TATCCTGTCC GTCCAGCAGT TCACCAAGGA TCAGATGTCT CACCTGTTCA ATGTGGCACA CACACTGCGT ATGATGGTGC AGAAGGAGCG GAGCCTCGAC ATCCTGAAGG GGAAGGTCAT GGCCTCCATG TTCTATGAAG TGAGCACACG GACCAGCAGC TCCTTTGCAG CAGCCATGGC CCGGCTGGGA GGTGCTGTGC TCAGCTTCTC GGAAGCCACA TCGTCCGTCC AGAAGGGCGA ATCCCTGGCT GACTCCGTGC AGACCATGAG CTGCTATGCC GACGTCGTCG TGCTCCGGCA CCCCCAGCCT GGAGCAGTGG AGCTGGCCGC CAAGCACTGC CGGAGGCCAG TGATCAATGC TGGGGATGGG GTCGGAGAGC ACCCCACCCA GGCCCTGCTG GACATCTTCA CCATCCGTGA GGAGCTGGGA ACTGTCAATG GCATGACGAT CACGATGGTG GGTGACCTGA AGCACGGACG CACAGTACAT TCCCTGGCCT GCCTGCTCAC CCAGTATCGT GTCAGCCTGC GCTACGTGGC ACCTCCCAGC CTGCGCATGC CACCCACTGT GCGGGCCTTC GTGGCCTCCC GCGGCACCAA GCAGGAGGAA TTCGAGAGCA TTGAGGAGGC GCTGCCTGAC ACTGATGTGC TCTACATGAC TCGAATCCAG AAGGAACGAT TTGGCTCTAC CCAGGAGTAC GAAGCTTGCT TTGGTCAGTT CATCCTCACT CCCCACATCA TGACCCGGGC CAAGAAGAAG ATGGTGGTGA TGCACCCGAT GCCCCGTGTC AACGAGATAA GCGTGGAAGT GGACTCGGAT CCCCGCGCAG CCTACTTCCG CCAGGCTGAG AACGGCATGT ACATCCGCAT GGCTCTGTTA GCCACCGTGC TGGGCCGTTT CTAGGCGGCC GCAAGGATCT GCGATCGCTC CGGTGCCCGT CAGTGGGCAG AGCGCACATC GCCCACAGTC CCCGAGAAGT TGGGGGGAGG GGTCGGCAAT TGAACGGGTG CCTAGAGAAG GTGGCGCGGG GTAAACTGGG AAAGTGATGT CGTGTACTGG CTCCGCCTTT TTCCCGAGGG TGGGGGAGAA CCGTATATAA GTGCAGTAGT CGCCGTGAAC GTTCTTTTTC GCAACGGGTT TGCCGCCAGA ACACAGCTGA AGCTTCGAGG GGCTCGCATC TCTCCTTCAC GCGCCCGCCG CCCTACCTGA GGCCGCCATC CACGCCGGTT GAGTCGCGTT CTGCCGCCTC CCGCCTGTGG TGCCTCCTGA ACTGCGTCCG CCGTCTAGGT AAGTTTAAAG CTCAGGTCGA GACCGGGCCT TTGTCCGGCG CTCCCTTGGA GCCTACCTAG ACTCAGCCGG CTCTCCACGC TTTGCCTGAC CCTGCTTGCT CAACTCTACG TCTTTGTTTC GTTTTCTGTT CTGCGCCGTT ACAGATCCAA GCTGTGACCG GCGCCTACGC TAGACGCCAC CATGGAGAGC GACGAGAGCG GCCTGATGGA AGACGCCAAA AACATAAAGA AAGGCCCGGC GCCATTCTAT CCGCTGGAAG ATGGAACCGC TGGAGAGCAA CTGCATAAGG CTATGAAGAG ATACGCCCTG GTTCCTGGAA CAATTGCTTT TACAGATGCA CATATCGAGG TGGACATCAC TTACGCTGAG TACTTCGAAA TGTCCGTTCG GTTGGCAGAA GCTATGAAAC GATATGGGCT GAATACAAAT CACAGAATCG TCGTATGCAG TGAAAACTCT CTTCAATTCT TTATGCCGGT GTTGGGCGCG TTATTTATCG GAGTTGCAGT TGCGCCCGCG AACGACATTT ATAATGAACG TGAATTGCTC AACAGTATGG GCATTTCGCA GCCTACCGTG GTGTTCGTTT CCAAAAAGGG GTTGCAAAAA ATTTTGAACG TGCAAAAAAA GCTCCCAATC ATCCAAAAAA TTATTATCAT GGATTCTAAA ACGGATTACC AGGGATTTCA GTCGATGTAC ACGTTCGTCA CATCTCATCT ACCTCCCGGT TTTAATGAAT ACGATTTTGT GCCAGAGTCC TTCGATAGGG ACAAGACAAT TGCACTGATC ATGAACTCCT CTGGATCTAC TGGTCTGCCT AAAGGTGTCG CTCTGCCTCA TAGAACTGCC TGCGTGAGAT TCTCGCATGC CAGAGATCCT ATTTTTGGCA ATCAAATCAT TCCGGATACT GCGATTTTAA GTGTTGTTCC ATTCCATCAC GGTTTTGGAA TGTTTACTAC ACTCGGATAT TTGATATGTG GATTTCGAGT CGTCTTAATG TATAGATTTG AAGAAGAGCT GTTTCTGAGG AGCCTTCAGG ATTACAAGAT TCAAAGTGCG CTGCTGGTGC CAACCCTATT CTCCTTCTTC GCCAAAAGCA CTCTGATTGA CAAATACGAT TTATCTAATT TACACGAAAT TGCTTCTGGT GGCGCTCCCC TCTCTAAGGA AGTCGGGGAA GCGGTTGCCA AGAGGTTCCA TCTGCCAGGT ATCAGGCAAG GATATGGGCT CACTGAGACT ACATCAGCTA TTCTGATTAC ACCCGAGGGG GATGATAAAC CGGGCGCGGT CGGTAAAGTT GTTCCATTTT TTGAAGCGAA GGTTGTGGAT CTGGATACCG GGAAAACGCT GGGCGTTAAT CAAAGAGGCG AACTGTGTGT GAGAGGTCCT ATGATTATGT CCGGTTATGT AAACAATCCG GAAGCGACCA ACGCCTTGAT TGACAAGGAT GGATGGCTAC ATTCTGGAGA CATAGCTTAC TGGGACGAAG ACGAACACTT CTTCATCGTT GACCGCCTGA AGTCTCTGAT TAAGTACAAA GGCTATCAGG TGGCTCCCGC TGAATTGGAA TCCATCTTGC TCCAACACCC CAACATCTTC GACGCAGGTG TCGCAGGTCT TCCCGACGAT GACGCCGGTG AACTTCCCGC CGCCGTTGTT GTTTTGGAGC ACGGAAAGAC GATGACGGAA AAAGAGATCG TGGATTACGT CGCCAGTCAA GTAACAACCG CGAAAAAGTT GCGCGGAGGA GTTGTGTTTG TGGACGAAGT ACCGAAAGGT CTTACCGGAA AACTCGACGC AAGAAAAATC AGAGAGATCC TCATAAAGGC CAAGAAGGGC GGAAAGATCG CCGTGAGATC CCGCGCTCAG TCGTCCAATT CTGCCGTGGA CGGCACCGCC GGACCCGGCT CCACCGGATC TCGCGAGGGC AGAGGAAGTC TTCTAACATG CGGTGACGTG GAGGAGAATC CCGGCCCTAT GACCGAGTAC AAGCCCACGG TGCGCCTCGC CACCCGCGAC GACGTCCCCA GGGCCGTACG CACCCTCGCC GCCGCGTTCG CCGACTACCC CGCCACGCGC CACACCGTCG ATCCGGACCG CCACATCGAG CGGGTCACCG AGCTGCAAGA ACTCTTCCTC ACGCGCGTCG GGCTCGACAT CGGCAAGGTG TGGGTCGCGG ACGACGGCGC CGCGGTGGCG GTCTGGACCA CGCCGGAGAG CGTCGAAGCG GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC GCGCAGCAAC AGATGGAAGG CCTCCTGGCG CCGCACCGGC CCAAGGAGCC CGCGTGGTTC CTGGCCACCG TCGGCGTCTC GCCCGACCAC CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG CTCCCCGGAG TGGAGGCGGC CGAGCGCGCC GGGGTGCCCG CCTTCCTGGA GACCTCCGCG CCCCGCAACC TCCCCTTCTA CGAGCGGCTC GGCTTCACCG TCACCGCCGA CGTCGAGGTG CCCGAAGGAC CGCGCACCTG GTGCATGACC CGCAAGCCCG GTGCCTGAAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAGCTGACG TCCTTTCCAT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTCCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGCCGCCT CCCCGCCTGG TACCTTTAAG ACCAATGACT TACAAGGCAG CTGTAGATCT TAGCCACTTT TTAAAAGAAA AGGGGGGACT GGAAGGGCTA ATTCACTCCC AACGAAAATA AGATCTGCTT TTTGCTTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA GTCAGTGTGG AAAATCTCTA GCAGTAGTAG TTCATGTCAT CTTATTATTC AGTATTTATA ACTTGCAAAG AAATGAATAT CAGAGAGTGA GAGGAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGCTCT AGCTATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGACTTTTG CAGAGACGGC CCAAATTCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGCCAAG CTG
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