pKSE401-EGFP
$70.00
In stock
SKU
PL29539
100μg liquid plasmid(Instantly):$113
| name | pKSE401-EGFP | another name | apKSE401-EGFP |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Empty vector | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | Green |
| Promoter | AtU6-26p | Replicon | pVS1 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pKSE401 | 5′ sequencing primer | |
| 3′ sequencing primer | Copy number | Low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAAACGCTCT TTTCTCTTAG GTTTACCCGC CAATATATCC TGTCAAACAC TGATAGTTTA AACTGAAGGC GGGAAACGAC AATCTGATCC AAGCTCAAGC TGCTCTAGCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGCCAAG CTTCGACTTG CCTTCCGCAC AATACATCAT TTCTTCTTAG CTTTTTTTCT TCTTCTTCGT TCATACAGTT TTTTTTTGTT TATCAGCTTA CATTTTCTTG AACCGTAGCT TTCGTTTTCT TCTTTTTAAC TTTCCATTCG GAGTTTTTGT ATCTTGTTTC ATAGTTTGTC CCAGGATTAG AATGATTAGG CATCGAACCT TCAAGAATTT GATTGAATAA AACATCTTCA TTCTTAAGAT ATGAAGATAA TCTTCAAAAG GCCCCTGGGA ATCTGAAAGA AGAGAAGCAG GCCCATTTAT ATGGGAAAGA ACAATAGTAT TTCTTATATA GGCCCATTTA AGTTGAAAAC AATCTTCAAA AGTCCCACAT CGCTTAGATA AGAAAACGAA GCTGAGTTTA TATACAGCTA GAGTCGAAGT AGTGATTGGG AGACCAACCC AGTGGACATA AGCCTGTTCG GTTCGTAAGC TGTAATGCAA GTAGCGTATG CGCTCACGCA ACTGGTCCAG AACCTTGACC GAACGCAGCG GTGGTAACGG CGCAGTGGCG GTTTTCATGG CTTGTTATGA CTGTTTTTTT GGGGTACAGT CTATGCCTCG GGCATCCAAG CAGCAAGCGC GTTACGCCGT GGGTCGATGT TTGATGTTAT GGAGCAGCAA CGATGTTACG CAGCAGGGCA GTCGCCCTAA AACAAAGTTA AACATCATGG GGGAAGCGGT GATCGCCGAA GTATCGACTC AACTATCAGA GGTAGTTGGC GTCATCGAGC GCCATCTCGA ACCGACGTTG CTGGCCGTAC ATTTGTACGG CTCCGCAGTG GATGGCGGCC TGAAGCCACA CAGTGATATT GATTTGCTGG TTACGGTGAC CGTAAGGCTT GATGAAACAA CGCGGCGAGC TTTGATCAAC GACCTTTTGG AAACTTCGGC TTCCCCTGGA GAGAGCGAGA TTCTCCGCGC TGTAGAAGTC ACCATTGTTG TGCACGACGA CATCATTCCG TGGCGTTATC CAGCTAAGCG CGAACTGCAA TTTGGAGAAT GGCAGCGCAA TGACATTCTT GCAGGTATCT TCGAGCCAGC CACGATCGAC ATTGATCTGG CTATCTTGCT GACAAAAGCA AGAGAACATA GCGTTGCCTT GGTAGGTCCA GCGGCGGAGG AACTCTTTGA TCCGGTTCCT GAACAGGATC TATTTGAGGC GCTAAATGAA ACCTTAACGC TATGGAACTC GCCGCCCGAC TGGGCTGGCG ATGAGCGAAA TGTAGTGCTT ACGTTGTCCC GCATTTGGTA CAGCGCAGTA ACCGGCAAAA TCGCGCCGAA GGATGTCGCT GCCGACTGGG CAATGGAGCG CCTGCCGGCC CAGTATCAGC CCGTCATACT TGAAGCTAGA CAGGCTTATC TTGGACAAGA AGAAGATCGC TTGGCCTCGC GCGCAGATCA GTTGGAAGAA TTTGTCCACT ACGTGAAAGG CGAGATCACC AAGGTAGTCG GCAAATAATG TCTAGCTAGA AATTCGTTCA AGCCGACGCC GCTTCGCGGC GCGGCTTAAC TCAAGCGTTA GATGCACTAA GCACATAATT GCTCACAGCC AAACTATCAG GTCAAGTCTG CTTTTATTAT TTTTAAGCGT GCATAATAAG CCGGTCTCGG TTTTAGAGCT AGAAATAGCA AGTTAAAATA AGGCTAGTCC GTTATCAACT TGAAAAAGTG GCACCGAGTC GGTGCTTTTT TTTGCAAAAT TTTCCAGATC GATTTCTTCT TCCTCTGTTC TTCGGCGTTC AATTTCTGGG GTTTTCTCTT CGTTTTCTGT AACTGAAACC TAAAATTTGA CCTAAAAAAA ATCTCAAATA ATATGATTCA GTGGTTTTGT ACTTTTCAGT TAGTTGAGTT TTGCAGTTCC GATGAGATAA ACCAATAAGC TTGCATGCCT GCAGGTCAAC ATGGTGGAGC ACGACACACT TGTCTACTCC AAAAATATCA AAGATACAGT CTCAGAAGAC CAAAGGGCAA TTGAGACTTT TCAACAAAGG GTAATATCCG GAAACCTCCT CGGATTCCAT TGCCCAGCTA TCTGTCACTT TATTGTGAAG ATAGTGGAAA AGGAAGGTGG CTCCTACAAA TGCCATCATT GCGATAAAGG AAAGGCCATC GTTGAAGATG CCTCTGCCGA CAGTGGTCCC AAAGATGGAC CCCCACCCAC GAGGAGCATC GTGGAAAAAG AAGACGTTCC AACCACGTCT TCAAAGCAAG TGGATTGATG TGATAACATG GTGGAGCACG ACACACTTGT CTACTCCAAA AATATCAAAG ATACAGTCTC AGAAGACCAA AGGGCAATTG AGACTTTTCA ACAAAGGGTA ATATCCGGAA ACCTCCTCGG ATTCCATTGC CCAGCTATCT GTCACTTTAT TGTGAAGATA GTGGAAAAGG AAGGTGGCTC CTACAAATGC CATCATTGCG ATAAAGGAAA GGCCATCGTT GAAGATGCCT CTGCCGACAG TGGTCCCAAA GATGGACCCC CACCCACGAG GAGCATCGTG GAAAAAGAAG ACGTTCCAAC CACGTCTTCA AAGCAAGTGG ATTGATGTGA TATCTCCACT GACGTAAGGG ATGACGCACA ATCCCACTAT CCTTCGCAAG ACCCTTCCTC TATATAAGGA AGTTCATTTC ATTTGGAGAG GACCTCGACC TCAACACAAC ATATACAAAA CAAACGAATC TCAAGCAATC AAGCATTCTA CTTCTATTGC AGCAATTTAA ATCATTTCTT TTAAAGCAAA AGCAATTTTC TGAAAATTTT CACCATTTAC GAACGATACT CGAGTAATCT AGATGGATTA CAAGGACCAC GACGGGGATT ACAAGGACCA CGACATTGAT TACAAGGATG ATGATGACAA GATGGCTCCG AAGAAGAAGA GGAAGGTTGG CATCCACGGG GTGCCAGCTG CTGACAAGAA GTACTCGATC GGCCTCGATA TTGGGACTAA CTCTGTTGGC TGGGCCGTGA TCACCGACGA GTACAAGGTG CCCTCAAAGA AGTTCAAGGT CCTGGGCAAC ACCGATCGGC ATTCCATCAA GAAGAATCTC ATTGGCGCTC TCCTGTTCGA CAGCGGCGAG ACGGCTGAGG CTACGCGGCT CAAGCGCACC GCCCGCAGGC GGTACACGCG CAGGAAGAAT CGCATCTGCT ACCTGCAGGA GATTTTCTCC AACGAGATGG CGAAGGTTGA CGATTCTTTC TTCCACAGGC TGGAGGAGTC ATTCCTCGTG GAGGAGGATA AGAAGCACGA GCGGCATCCA ATCTTCGGCA ACATTGTCGA CGAGGTTGCC TACCACGAGA AGTACCCTAC GATCTACCAT CTGCGGAAGA AGCTCGTGGA CTCCACAGAT AAGGCGGACC TCCGCCTGAT CTACCTCGCT CTGGCCCACA TGATTAAGTT CAGGGGCCAT TTCCTGATCG AGGGGGATCT CAACCCGGAC AATAGCGATG TTGACAAGCT GTTCATCCAG CTCGTGCAGA CGTACAACCA GCTCTTCGAG GAGAACCCCA TTAATGCGTC AGGCGTCGAC GCGAAGGCTA TCCTGTCCGC TAGGCTCTCG AAGTCTCGGC GCCTCGAGAA CCTGATCGCC CAGCTGCCGG GCGAGAAGAA GAACGGCCTG TTCGGGAATC TCATTGCGCT CAGCCTGGGG CTCACGCCCA ACTTCAAGTC GAATTTCGAT CTCGCTGAGG ACGCCAAGCT GCAGCTCTCC AAGGACACAT ACGACGATGA CCTGGATAAC CTCCTGGCCC AGATCGGCGA TCAGTACGCG GACCTGTTCC TCGCTGCCAA GAATCTGTCG GACGCCATCC TCCTGTCTGA TATTCTCAGG GTGAACACCG AGATTACGAA GGCTCCGCTC TCAGCCTCCA TGATCAAGCG CTACGACGAG CACCATCAGG ATCTGACCCT CCTGAAGGCG CTGGTCAGGC AGCAGCTCCC CGAGAAGTAC AAGGAGATCT TCTTCGATCA GTCGAAGAAC GGCTACGCTG GGTACATTGA CGGCGGGGCC TCTCAGGAGG AGTTCTACAA GTTCATCAAG CCGATTCTGG AGAAGATGGA CGGCACGGAG GAGCTGCTGG TGAAGCTCAA TCGCGAGGAC CTCCTGAGGA AGCAGCGGAC ATTCGATAAC GGCAGCATCC CACACCAGAT TCATCTCGGG GAGCTGCACG CTATCCTGAG GAGGCAGGAG GACTTCTACC CTTTCCTCAA GGATAACCGC GAGAAGATCG AGAAGATTCT GACTTTCAGG ATCCCGTACT ACGTCGGCCC ACTCGCTAGG GGCAACTCCC GCTTCGCTTG GATGACCCGC AAGTCAGAGG AGACGATCAC GCCGTGGAAC TTCGAGGAGG TGGTCGACAA GGGCGCTAGC GCTCAGTCGT TCATCGAGAG GATGACGAAT TTCGACAAGA ACCTGCCAAA TGAGAAGGTG CTCCCTAAGC ACTCGCTCCT GTACGAGTAC TTCACAGTCT ACAACGAGCT GACTAAGGTG AAGTATGTGA CCGAGGGCAT GAGGAAGCCG GCTTTCCTGT CTGGGGAGCA GAAGAAGGCC ATCGTGGACC TCCTGTTCAA GACCAACCGG AAGGTCACGG TTAAGCAGCT CAAGGAGGAC TACTTCAAGA AGATTGAGTG CTTCGATTCG GTCGAGATCT CTGGCGTTGA GGACCGCTTC AACGCCTCCC TGGGGACCTA CCACGATCTC CTGAAGATCA TTAAGGATAA GGACTTCCTG GACAACGAGG AGAATGAGGA TATCCTCGAG GACATTGTGC TGACACTCAC TCTGTTCGAG GACCGGGAGA TGATCGAGGA GCGCCTGAAG ACTTACGCCC ATCTCTTCGA TGACAAGGTC ATGAAGCAGC TCAAGAGGAG GAGGTACACC GGCTGGGGGA GGCTGAGCAG GAAGCTCATC AACGGCATTC GGGACAAGCA GTCCGGGAAG ACGATCCTCG ACTTCCTGAA GAGCGATGGC TTCGCGAACC GCAATTTCAT GCAGCTGATT CACGATGACA GCCTCACATT CAAGGAGGAT ATCCAGAAGG CTCAGGTGAG CGGCCAGGGG GACTCGCTGC ACGAGCATAT CGCGAACCTC GCTGGCTCGC CAGCTATCAA GAAGGGGATT CTGCAGACCG TGAAGGTTGT GGACGAGCTG GTGAAGGTCA TGGGCAGGCA CAAGCCTGAG AACATCGTCA TTGAGATGGC CCGGGAGAAT CAGACCACGC AGAAGGGCCA GAAGAACTCA CGCGAGAGGA TGAAGAGGAT CGAGGAGGGC ATTAAGGAGC TGGGGTCCCA GATCCTCAAG GAGCACCCGG TGGAGAACAC GCAGCTGCAG AATGAGAAGC TCTACCTGTA CTACCTCCAG AATGGCCGCG ATATGTATGT GGACCAGGAG CTGGATATTA ACAGGCTCAG CGATTACGAC GTCGATCATA TCGTTCCACA GTCATTCCTG AAGGATGACT CCATTGACAA CAAGGTCCTC ACCAGGTCGG ACAAGAACCG GGGCAAGTCT GATAATGTTC CTTCAGAGGA GGTCGTTAAG AAGATGAAGA ACTACTGGCG CCAGCTCCTG AATGCCAAGC TGATCACGCA GCGGAAGTTC GATAACCTCA CAAAGGCTGA GAGGGGCGGG CTCTCTGAGC TGGACAAGGC GGGCTTCATC AAGAGGCAGC TGGTCGAGAC ACGGCAGATC ACTAAGCACG TTGCGCAGAT TCTCGACTCA CGGATGAACA CTAAGTACGA TGAGAATGAC AAGCTGATCC GCGAGGTGAA GGTCATCACC CTGAAGTCAA AGCTCGTCTC CGACTTCAGG AAGGATTTCC AGTTCTACAA GGTTCGGGAG ATCAACAATT ACCACCATGC CCATGACGCG TACCTGAACG CGGTGGTCGG CACAGCTCTG ATCAAGAAGT ACCCAAAGCT CGAGAGCGAG TTCGTGTACG GGGACTACAA GGTTTACGAT GTGAGGAAGA TGATCGCCAA GTCGGAGCAG GAGATTGGCA AGGCTACCGC CAAGTACTTC TTCTACTCTA ACATTATGAA TTTCTTCAAG ACAGAGATCA CTCTGGCCAA TGGCGAGATC CGGAAGCGCC CCCTCATCGA GACGAACGGC GAGACGGGGG AGATCGTGTG GGACAAGGGC AGGGATTTCG CGACCGTCAG GAAGGTTCTC TCCATGCCAC AAGTGAATAT CGTCAAGAAG ACAGAGGTCC AGACTGGCGG GTTCTCTAAG GAGTCAATTC TGCCTAAGCG GAACAGCGAC AAGCTCATCG CCCGCAAGAA GGACTGGGAT CCGAAGAAGT ACGGCGGGTT CGACAGCCCC ACTGTGGCCT ACTCGGTCCT GGTTGTGGCG AAGGTTGAGA AGGGCAAGTC CAAGAAGCTC AAGAGCGTGA AGGAGCTGCT GGGGATCACG ATTATGGAGC GCTCCAGCTT CGAGAAGAAC CCGATCGATT TCCTGGAGGC GAAGGGCTAC AAGGAGGTGA AGAAGGACCT GATCATTAAG CTCCCCAAGT ACTCACTCTT CGAGCTGGAG AACGGCAGGA AGCGGATGCT GGCTTCCGCT GGCGAGCTGC AGAAGGGGAA CGAGCTGGCT CTGCCGTCCA AGTATGTGAA CTTCCTCTAC CTGGCCTCCC ACTACGAGAA GCTCAAGGGC AGCCCCGAGG ACAACGAGCA GAAGCAGCTG TTCGTCGAGC AGCACAAGCA TTACCTCGAC GAGATCATTG AGCAGATTTC CGAGTTCTCC AAGCGCGTGA TCCTGGCCGA CGCGAATCTG GATAAGGTCC TCTCCGCGTA CAACAAGCAC CGCGACAAGC CAATCAGGGA GCAGGCTGAG AATATCATTC ATCTCTTCAC CCTGACGAAC CTCGGCGCCC CTGCTGCTTT CAAGTACTTC GACACAACTA TCGATCGCAA GAGGTACACA AGCACTAAGG AGGTCCTGGA CGCGACCCTC ATCCACCAGT CGATTACCGG CCTCTACGAG ACGCGCATCG ACCTGTCTCA GCTCGGGGGC GACAAGCGGC CAGCGGCGAC GAAGAAGGCG GGGCAGGCGA AGAAGAAGAA GGGATCCGGC GCAACAAACT TCTCTCTGCT GAAACAAGCC GGAGATGTCG AAGAGAATCC TGGACCGATG GTGTCTAAGG GCGAAGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTGAGCT CAGAGCTTTC GTTCGTATCA TCGGTTTCGA CAACGTTCGT CAAGTTCAAT GCATCAGTTT CATTGCGCAC ACACCAGAAT CCTACTGAGT TTGAGTATTA TGGCATTGGG AAAACTGTTT TTCTTGTACC ATTTGTTGTG CTTGTAATTT ACTGTGTTTT TTATTCGGTT TTCGCTATCG AACTGTGAAA TGGAAATGGA TGGAGAAGAG TTAATGAATG ATATGGTCCT TTTGTTCATT CTCAAATTAA TATTATTTGT TTTTTCTCTT ATTTGTTGTG TGTTGAATTT GAAATTATAA GAGATATGCA AACATTTTGT TTTGAGTAAA AATGTGTCAA ATCGTGGCCT CTAATGACCG AAGTTAATAT GAGGAGTAAA ACACTTGTAG TTGTACCATT ATGCTTATTC ACTAGGCAAC AAATATATTT TCAGACCTAG AAAAGCTGCA AATGTTACTG AATACAAGTA TGTCCTCTTG TGTTTTAGAC ATTTATGAAC TTTCCTTTAT GTAATTTTCC AGAATCCTTG TCAGATTCTA ATCATTGCTT TATAATTATA GTTATACTCA TGGATTTGTA GTTGAGTATG AAAATATTTT TTAATGCATT TTATGACTTG CCAATTGATT GACAACGAAT TCGTAATCAT GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGCTAG AGCAGCTTGC CAACATGGTG GAGCACGACA CTCTCGTCTA CTCCAAGAAT ATCAAAGATA CAGTCTCAGA AGACCAAAGG GCTATTGAGA CTTTTCAACA AAGGGTAATA TCGGGAAACC TCCTCGGATT CCATTGCCCA GCTATCTGTC ACTTCATCAA AAGGACAGTA GAAAAGGAAG GTGGCACCTA CAAATGCCAT CATTGCGATA AAGGAAAGGC TATCGTTCAA GATGCCTCTG CCGACAGTGG TCCCAAAGAT GGACCCCCAC CCACGAGGAG CATCGTGGAA AAAGAAGACG TTCCAACCAC GTCTTCAAAG CAAGTGGATT GATGTGAACA TGGTGGAGCA CGACACTCTC GTCTACTCCA AGAATATCAA AGATACAGTC TCAGAAGACC AAAGGGCTAT TGAGACTTTT CAACAAAGGG TAATATCGGG AAACCTCCTC GGATTCCATT GCCCAGCTAT CTGTCACTTC ATCAAAAGGA CAGTAGAAAA GGAAGGTGGC ACCTACAAAT GCCATCATTG CGATAAAGGA AAGGCTATCG TTCAAGATGC CTCTGCCGAC AGTGGTCCCA AAGATGGACC CCCACCCACG AGGAGCATCG TGGAAAAAGA AGACGTTCCA ACCACGTCTT CAAAGCAAGT GGATTGATGT GATATCTCCA CTGACGTAAG GGATGACGCA CAATCCCACT ATCCTTCGCA AGACCCTTCC TCTATATAAG GAAGTTCATT TCATTTGGAG AGGACACGCT GAAATCACCA GTCTCTCTCT ACAAATCTAT CTCTCTCGAG CTTTCGCAGA TCTGTCGATC GACCATGGGG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTTCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTCCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACACA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG GATCGATCCT CTAGCTAGAG TCGATCGACA AGCTCGAGTT TCTCCATAAT AATGTGTGAG TAGTTCCCAG ATAAGGGAAT TAGGGTTCCT ATAGGGTTTC GCTCATGTGT TGAGCATATA AGAAACCCTT AGTATGTATT TGTATTTGTA AAATACTTCT ATCAATAAAA TTTCTAATTC CTAAAACCAA AATCCAGTAC TAAAATCCAG ATCCCCCGAA TTAATTCGGC GTTAATTCAG TACATTAAAA ACGTCCGCAA TGTGTTATTA AGTTGTCTAA GCGTCAATTT GTTTACACCA CAATATATCC TGCCACCAGC CAGCCAACAG CTCCCCGACC GGCAGCTCGG CACAAAATCA CCACTCGATA CAGGCAGCCC ATCAGTCCGG GACGGCGTCA GCGGGAGAGC CGTTGTAAGG CGGCAGACTT TGCTCATGTT ACCGATGCTA TTCGGAAGAA CGGCAACTAA GCTGCCGGGT TTGAAACACG GATGATCTCG CGGAGGGTAG CATGTTGATT GTAACGATGA CAGAGCGTTG CTGCCTGTGA TCACCGCGGT TTCAAAATCG GCTCCGTCGA TACTATGTTA TACGCCAACT TTGAAAACAA CTTTGAAAAA GCTGTTTTCT GGTATTTAAG GTTTTAGAAT GCAAGGAACA GTGAATTGGA GTTCGTCTTG TTATAATTAG CTTCTTGGGG TATCTTTAAA TACTGTAGAA AAGAGGAAGG AAATAATAAA TGGCTAAAAT GAGAATATCA CCGGAATTGA AAAAACTGAT CGAAAAATAC CGCTGCGTAA AAGATACGGA AGGAATGTCT CCTGCTAAGG TATATAAGCT GGTGGGAGAA AATGAAAACC TATATTTAAA AATGACGGAC AGCCGGTATA AAGGGACCAC CTATGATGTG GAACGGGAAA AGGACATGAT GCTATGGCTG GAAGGAAAGC TGCCTGTTCC AAAGGTCCTG CACTTTGAAC GGCATGATGG CTGGAGCAAT CTGCTCATGA GTGAGGCCGA TGGCGTCCTT TGCTCGGAAG AGTATGAAGA TGAACAAAGC CCTGAAAAGA TTATCGAGCT GTATGCGGAG TGCATCAGGC TCTTTCACTC CATCGACATA TCGGATTGTC CCTATACGAA TAGCTTAGAC AGCCGCTTAG CCGAATTGGA TTACTTACTG AATAACGATC TGGCCGATGT GGATTGCGAA AACTGGGAAG AAGACACTCC ATTTAAAGAT CCGCGCGAGC TGTATGATTT TTTAAAGACG GAAAAGCCCG AAGAGGAACT TGTCTTTTCC CACGGCGACC TGGGAGACAG CAACATCTTT GTGAAAGATG GCAAAGTAAG TGGCTTTATT GATCTTGGGA GAAGCGGCAG GGCGGACAAG TGGTATGACA TTGCCTTCTG CGTCCGGTCG ATCAGGGAGG ATATCGGGGA AGAACAGTAT GTCGAGCTAT TTTTTGACTT ACTGGGGATC AAGCCTGATT GGGAGAAAAT AAAATATTAT ATTTTACTGG ATGAATTGTT TTAGTACCTA GAATGCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGGGTG CCTTGATGTG GGCGCCGGCG GTCGAGTGGC GACGGCGCGG CTTGTCCGCG CCCTGGTAGA TTGCCTGGCC GTAGGCCAGC CATTTTTGAG CGGCCAGCGG CCGCGATAGG CCGACGCGAA GCGGCGGGGC GTAGGGAGCG CAGCGACCGA AGGGTAGGCG CTTTTTGCAG CTCTTCGGCT GTGCGCTGGC CAGACAGTTA TGCACAGGCC AGGCGGGTTT TAAGAGTTTT AATAAGTTTT AAAGAGTTTT AGGCGGAAAA ATCGCCTTTT TTCTCTTTTA TATCAGTCAC TTACATGTGT GACCGGTTCC CAATGTACGG CTTTGGGTTC CCAATGTACG GGTTCCGGTT CCCAATGTAC GGCTTTGGGT TCCCAATGTA CGTGCTATCC ACAGGAAACA GACCTTTTCG ACCTTTTTCC CCTGCTAGGG CAATTTGCCC TAGCATCTGC TCCGTACATT AGGAACCGGC GGATGCTTCG CCCTCGATCA GGTTGCGGTA GCGCATGACT AGGATCGGGC CAGCCTGCCC CGCCTCCTCC TTCAAATCGT ACTCCGGCAG GTCATTTGAC CCGATCAGCT TGCGCACGGT GAAACAGAAC TTCTTGAACT CTCCGGCGCT GCCACTGCGT TCGTAGATCG TCTTGAACAA CCATCTGGCT TCTGCCTTGC CTGCGGCGCG GCGTGCCAGG CGGTAGAGAA AACGGCCGAT GCCGGGATCG ATCAAAAAGT AATCGGGGTG AACCGTCAGC ACGTCCGGGT TCTTGCCTTC TGTGATCTCG CGGTACATCC AATCAGCTAG CTCGATCTCG ATGTACTCCG GCCGCCCGGT TTCGCTCTTT ACGATCTTGT AGCGGCTAAT CAAGGCTTCA CCCTCGGATA CCGTCACCAG GCGGCCGTTC TTGGCCTTCT TCGTACGCTG CATGGCAACG TGCGTGGTGT TTAACCGAAT GCAGGTTTCT ACCAGGTCGT CTTTCTGCTT TCCGCCATCG GCTCGCCGGC AGAACTTGAG TACGTCCGCA ACGTGTGGAC GGAACACGCG GCCGGGCTTG TCTCCCTTCC CTTCCCGGTA TCGGTTCATG GATTCGGTTA GATGGGAAAC CGCCATCAGT ACCAGGTCGT AATCCCACAC ACTGGCCATG CCGGCCGGCC CTGCGGAAAC CTCTACGTGC CCGTCTGGAA GCTCGTAGCG GATCACCTCG CCAGCTCGTC GGTCACGCTT CGACAGACGG AAAACGGCCA CGTCCATGAT GCTGCGACTA TCGCGGGTGC CCACGTCATA GAGCATCGGA ACGAAAAAAT CTGGTTGCTC GTCGCCCTTG GGCGGCTTCC TAATCGACGG CGCACCGGCT GCCGGCGGTT GCCGGGATTC TTTGCGGATT CGATCAGCGG CCGCTTGCCA CGATTCACCG GGGCGTGCTT CTGCCTCGAT GCGTTGCCGC TGGGCGGCCT GCGCGGCCTT CAACTTCTCC ACCAGGTCAT CACCCAGCGC CGCGCCGATT TGTACCGGGC CGGATGGTTT GCGACCGCTC ACGCCGATTC CTCGGGCTTG GGGGTTCCAG TGCCATTGCA GGGCCGGCAG GCAACCCAGC CGCTTACGCC TGGCCAACCG CCCGTTCCTC CACACATGGG GCATTCCACG GCGTCGGTGC CTGGTTGTTC TTGATTTTCC ATGCCGCCTC CTTTAGCCGC TAAAATTCAT CTACTCATTT ATTCATTTGC TCATTTACTC TGGTAGCTGC GCGATGTATT CAGATAGCAG CTCGGTAATG GTCTTGCCTT GGCGTACCGC GTACATCTTC AGCTTGGTGT GATCCTCCGC CGGCAACTGA AAGTTGACCC GCTTCATGGC TGGCGTGTCT GCCAGGCTGG CCAACGTTGC AGCCTTGCTG CTGCGTGCGC TCGGACGGCC GGCACTTAGC GTGTTTGTGC TTTTGCTCAT TTTCTCTTTA CCTCATTAAC TCAAATGAGT TTTGATTTAA TTTCAGCGGC CAGCGCCTGG ACCTCGCGGG CAGCGTCGCC CTCGGGTTCT GATTCAAGAA CGGTTGTGCC GGCGGCGGCA GTGCCTGGGT AGCTCACGCG CTGCGTGATA CGGGACTCAA GAATGGGCAG CTCGTACCCG GCCAGCGCCT CGGCAACCTC ACCGCCGATG CGCGTGCCTT TGATCGCCCG CGACACGACA AAGGCCGCTT GTAGCCTTCC ATCCGTGACC TCAATGCGCT GCTTAACCAG CTCCACCAGG TCGGCGGTGG CCCATATGTC GTAAGGGCTT GGCTGCACCG GAATCAGCAC GAAGTCGGCT GCCTTGATCG CGGACACAGC CAAGTCCGCC GCCTGGGGCG CTCCGTCGAT CACTACGAAG TCGCGCCGGC CGATGGCCTT CACGTCGCGG TCAATCGTCG GGCGGTCGAT GCCGACAACG GTTAGCGGTT GATCTTCCCG CACGGCCGCC CAATCGCGGG CACTGCCCTG GGGATCGGAA TCGACTAACA GAACATCGGC CCCGGCGAGT TGCAGGGCGC GGGCTAGATG GGTTGCGATG GTCGTCTTGC CTGACCCGCC TTTCTGGTTA AGTACAGCGA TAACCTTCAT GCGTTCCCCT TGCGTATTTG TTTATTTACT CATCGCATCA TATACGCAGC GACCGCATGA CGCAAGCTGT TTTACTCAAA TACACATCAC CTTTTTAGAC GGCGGCGCTC GGTTTCTTCA GCGGCCAAGC TGGCCGGCCA GGCCGCCAGC TTGGCATCAG ACAAACCGGC CAGGATTTCA TGCAGCCGCA CGGTTGAGAC GTGCGCGGGC GGCTCGAACA CGTACCCGGC CGCGATCATC TCCGCCTCGA TCTCTTCGGT AATGAAAAAC GGTTCGTCCT GGCCGTCCTG GTGCGGTTTC ATGCTTGTTC CTCTTGGCGT TCATTCTCGG CGGCCGCCAG GGCGTCGGCC TCGGTCAATG CGTCCTCACG GAAGGCACCG CGCCGCCTGG CCTCGGTGGG CGTCACTTCC TCGCTGCGCT CAAGTGCGCG GTACAGGGTC GAGCGATGCA CGCCAAGCAG TGCAGCCGCC TCTTTCACGG TGCGGCCTTC CTGGTCGATC AGCTCGCGGG CGTGCGCGAT CTGTGCCGGG GTGAGGGTAG GGCGGGGGCC AAACTTCACG CCTCGGGCCT TGGCGGCCTC GCGCCCGCTC CGGGTGCGGT CGATGATTAG GGAACGCTCG AACTCGGCAA TGCCGGCGAA CACGGTCAAC ACCATGCGGC CGGCCGGCGT GGTGGTGTCG GCCCACGGCT CTGCCAGGCT ACGCAGGCCC GCGCCGGCCT CCTGGATGCG CTCGGCAATG TCCAGTAGGT CGCGGGTGCT GCGGGCCAGG CGGTCTAGCC TGGTCACTGT CACAACGTCG CCAGGGCGTA GGTGGTCAAG CATCCTGGCC AGCTCCGGGC GGTCGCGCCT GGTGCCGGTG ATCTTCTCGG AAAACAGCTT GGTGCAGCCG GCCGCGTGCA GTTCGGCCCG TTGGTTGGTC AAGTCCTGGT CGTCGGTGCT GACGCGGGCA TAGCCCAGCA GGCCAGCGGC GGCGCTCTTG TTCATGGCGT AATGTCTCCG GTTCTAGTCG CAAGTATTCT ACTTTATGCG ACTAAAACAC GCGACAAGAA AACGCCAGGA AAAGGGCAGG GCGGCAGCCT GTCGCGTAAC TTAGGACTTG TGCGACATGT CGTTTTCAGA AGACGGCTGC ACTGAACGTC AGAAGCCGAC TGCACTATAG CAGCGGAGGG GTTGGATCAA AGTACTTTGA TCCCGAGGGG AACCCTGTGG TTGGCATGCA CATACAAATG GACGAACGGA TAAACCTTTT CACGCCCTTT TAAATATCCG ATTATTCTAA
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