NM_001530.4;bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha;HIF1; HIF1-ALPHA; MOP1; PASD8
100μg liquid plasmid(Instantly):$105
| name | pCMV-DsRed2-HIF1A(human)-Neo | another name | apCMV-DsRed2-HIF1A(human)-Neo |
| Insert Size (bp) | 2481bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | Red |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pDsRed2-C1 | 5′ sequencing primer | |
| 3′ sequencing primer | HIF1A-R1： | Copy number | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGTCGCCA CCATGGCCTC CTCCGAGAAC GTCATCACCG AGTTCATGCG CTTCAAGGTG CGCATGGAGG GCACCGTGAA CGGCCACGAG TTCGAGATCG AGGGCGAGGG CGAGGGCCGC CCCTACGAGG GCCACAACAC CGTGAAGCTG AAGGTGACCA AGGGCGGCCC CCTGCCCTTC GCCTGGGACA TCCTGTCCCC CCAGTTCCAG TACGGCTCCA AGGTGTACGT GAAGCACCCC GCCGACATCC CCGACTACAA GAAGCTGTCC TTCCCCGAGG GCTTCAAGTG GGAGCGCGTG ATGAACTTCG AGGACGGCGG CGTGGCGACC GTGACCCAGG ACTCCTCCCT GCAGGACGGC TGCTTCATCT ACAAGGTGAA GTTCATCGGC GTGAACTTCC CCTCCGACGG CCCCGTGATG CAGAAGAAGA CCATGGGCTG GGAGGCCTCC ACCGAGCGCC TGTACCCCCG CGACGGCGTG CTGAAGGGCG AGACCCACAA GGCCCTGAAG CTGAAGGACG GCGGCCACTA CCTGGTGGAG TTCAAGTCCA TCTACATGGC CAAGAAGCCC GTGCAGCTGC CCGGCTACTA CTACGTGGAC GCCAAGCTGG ACATCACCTC CCACAACGAG GACTACACCA TCGTGGAGCA GTACGAGCGC ACCGAGGGCC GCCACCACCT GTTCCTGAGA TCTCGAGGAG GCGGAGGTAG TGGAGGAGGG GGAAGCGGAG GCGGAGGGAG TGAATTCATG GAGGGCGCCG GCGGCGCGAA CGACAAGAAA AAGATAAGTT CTGAACGTCG AAAAGAAAAG TCTCGAGATG CAGCCAGATC TCGGCGAAGT AAAGAATCTG AAGTTTTTTA TGAGCTTGCT CATCAGTTGC CACTTCCACA TAATGTGAGT TCGCATCTTG ATAAGGCCTC TGTGATGAGG CTTACCATCA GCTATTTGCG TGTGAGGAAA CTTCTGGATG CTGGTGATTT GGATATTGAA GATGACATGA AAGCACAGAT GAATTGCTTT TATTTGAAAG CCTTGGATGG TTTTGTTATG GTTCTCACAG ATGATGGTGA CATGATTTAC ATTTCTGATA ATGTGAACAA ATACATGGGA TTAACTCAGT TTGAACTAAC TGGACACAGT GTGTTTGATT TTACTCATCC ATGTGACCAT GAGGAAATGA GAGAAATGCT TACACACAGA AATGGCCTTG TGAAAAAGGG TAAAGAACAA AACACACAGC GAAGCTTTTT TCTCAGAATG AAGTGTACCC TAACTAGCCG AGGAAGAACT ATGAACATAA AGTCTGCAAC ATGGAAGGTA TTGCACTGCA CAGGCCACAT TCACGTATAT GATACCAACA GTAACCAACC TCAGTGTGGG TATAAGAAAC CACCTATGAC CTGCTTGGTG CTGATTTGTG AACCCATTCC TCACCCATCA AATATTGAAA TTCCTTTAGA TAGCAAGACT TTCCTCAGTC GACACAGCCT GGATATGAAA TTTTCTTATT GTGATGAAAG AATTACCGAA TTGATGGGAT ATGAGCCAGA AGAACTTTTA GGCCGCTCAA TTTATGAATA TTATCATGCT TTGGACTCTG ATCATCTGAC CAAAACTCAT CATGATATGT TTACTAAAGG ACAAGTCACC ACAGGACAGT ACAGGATGCT TGCCAAAAGA GGTGGATATG TCTGGGTTGA AACTCAAGCA ACTGTCATAT ATAACACCAA GAATTCTCAA CCACAGTGCA TTGTATGTGT GAATTACGTT GTGAGTGGTA TTATTCAGCA CGACTTGATT TTCTCCCTTC AACAAACAGA ATGTGTCCTT AAACCGGTTG AATCTTCAGA TATGAAAATG ACTCAGCTAT TCACCAAAGT TGAATCAGAA GATACAAGTA GCCTCTTTGA CAAACTTAAG AAGGAACCTG ATGCTTTAAC TTTGCTGGCC CCAGCCGCTG GAGACACAAT CATATCTTTA GATTTTGGCA GCAACGACAC AGAAACTGAT GACCAGCAAC TTGAGGAAGT ACCATTATAT AATGATGTAA TGCTCCCCTC ACCCAACGAA AAATTACAGA ATATAAATTT GGCAATGTCT CCATTACCCA CCGCTGAAAC GCCAAAGCCA CTTCGAAGTA GTGCTGACCC TGCACTCAAT CAAGAAGTTG CATTAAAATT AGAACCAAAT CCAGAGTCAC TGGAACTTTC TTTTACCATG CCCCAGATTC AGGATCAGAC ACCTAGTCCT TCCGATGGAA GCACTAGACA AAGTTCACCT GAGCCTAATA GTCCCAGTGA ATATTGTTTT TATGTGGATA GTGATATGGT CAATGAATTC AAGTTGGAAT TGGTAGAAAA ACTTTTTGCT GAAGACACAG AAGCAAAGAA CCCATTTTCT ACTCAGGACA CAGATTTAGA CTTGGAGATG TTAGCTCCCT ATATCCCAAT GGATGATGAC TTCCAGTTAC GTTCCTTCGA TCAGTTGTCA CCATTAGAAA GCAGTTCCGC AAGCCCTGAA AGCGCAAGTC CTCAAAGCAC AGTTACAGTA TTCCAGCAGA CTCAAATACA AGAACCTACT GCTAATGCCA CCACTACCAC TGCCACCACT GATGAATTAA AAACAGTGAC AAAAGACCGT ATGGAAGACA TTAAAATATT GATTGCATCT CCATCTCCTA CCCACATACA TAAAGAAACT ACTAGTGCCA CATCATCACC ATATAGAGAT ACTCAAAGTC GGACAGCCTC ACCAAACAGA GCAGGAAAAG GAGTCATAGA ACAGACAGAA AAATCTCATC CAAGAAGCCC TAACGTGTTA TCTGTCGCTT TGAGTCAAAG AACTACAGTT CCTGAGGAAG AACTAAATCC AAAGATACTA GCTTTGCAGA ATGCTCAGAG AAAGCGAAAA ATGGAACATG ATGGTTCACT TTTTCAAGCA GTAGGAATTG GAACATTATT ACAGCAGCCA GACGATCATG CAGCTACTAC ATCACTTTCT TGGAAACGTG TAAAAGGATG CAAATCTAGT GAACAGAATG GAATGGAGCA AAAGACAATT ATTTTAATAC CCTCTGATTT AGCATGTAGA CTGCTGGGGC AATCAATGGA TGAAAGTGGA TTACCACAGC TGACCAGTTA TGATTGTGAA GTTAATGCTC CTATACAAGG CAGCAGAAAC CTACTGCAGG GTGAAGAATT ACTCAGAGCT TTGGATCAAG TTAACTGAGG ATCCACCGGA TCTAGATAAC TGATCATAAT CAGCCATACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAA CGCGTAAATT GTAAGCGTTA ATATTTTGTT AAAATTCGCG TTAAATTTTT GTTAAATCAG CTCATTTTTT AACCAATAGG CCGAAATCGG CAAAATCCCT TATAAATCAA AAGAATAGAC CGAGATAGGG TTGAGTGTTG TTCCAGTTTG GAACAAGAGT CCACTATTAA AGAACGTGGA CTCCAACGTC AAAGGGCGAA AAACCGTCTA TCAGGGCGAT GGCCCACTAC GTGAACCATC ACCCTAATCA AGTTTTTTGG GGTCGAGGTG CCGTAAAGCA CTAAATCGGA ACCCTAAAGG GAGCCCCCGA TTTAGAGCTT GACGGGGAAA GCCGGCGAAC GTGGCGAGAA AGGAAGGGAA GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT GGCAAGTGTA GCGGTCACGC TGCGCGTAAC CACCACACCC GCCGCGCTTA ATGCGCCGCT ACAGGGCGCG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTCCTGAGGC GGAAAGAACC AGCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AGATCGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAAGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCTAGGGGGA GGCTAACTGA AACACGGAAG GAGACAATAC CGGAAGGAAC CCGCGCTATG ACGGCAATAA AAAGACAGAA TAAAACGCAC GGTGTTGGGT CGTTTGTTCA TAAACGCGGG GTTCGGTCCC AGGGCTGGCA CTCTGTCGAT ACCCCACCGA GACCCCATTG GGGCCAATAC GCCCGCGTTT CTTCCTTTTC CCCACCCCAC CCCCCAAGTT CGGGTGAAGG CCCAGGGCTC GCAGCCAACG TCGGGGCGGC AGGCCCTGCC ATAGCCTCAG GTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCA
