pTac-GST-HK2(human)
$84.00
In stock
SKU
PL29243
NM_000189.5;HKII;HXK2
100μg liquid plasmid(Instantly):$127
| name | pTac-GST-HK2(human) | another name | apTac-GST-HK2(human) |
| Insert Size (bp) | 2754bp | Plasmid host | Escherichia coli |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | Tac | Replicon | pBR322 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pGEX-4T-1 | 5′ sequencing primer | pGEX-3' |
| 3′ sequencing primer | Copy number | Low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACGTTATCGA CTGCACGGTG CACCAATGCT TCTGGCGTCA GGCAGCCATC GGAAGCTGTG GTATGGCTGT GCAGGTCGTA AATCACTGCA TAATTCGTGT CGCTCAAGGC GCACTCCCGT TCTGGATAAT GTTTTTTGCG CCGACATCAT AACGGTTCTG GCAAATATTC TGAAATGAGC TGTTGACAAT TAATCATCGG CTCGTATAAT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGTATTCATG TCCCCTATAC TAGGTTATTG GAAAATTAAG GGCCTTGTGC AACCCACTCG ACTTCTTTTG GAATATCTTG AAGAAAAATA TGAAGAGCAT TTGTATGAGC GCGATGAAGG TGATAAATGG CGAAACAAAA AGTTTGAATT GGGTTTGGAG TTTCCCAATC TTCCTTATTA TATTGATGGT GATGTTAAAT TAACACAGTC TATGGCCATC ATACGTTATA TAGCTGACAA GCACAACATG TTGGGTGGTT GTCCAAAAGA GCGTGCAGAG ATTTCAATGC TTGAAGGAGC GGTTTTGGAT ATTAGATACG GTGTTTCGAG AATTGCATAT AGTAAAGACT TTGAAACTCT CAAAGTTGAT TTTCTTAGCA AGCTACCTGA AATGCTGAAA ATGTTCGAAG ATCGTTTATG TCATAAAACA TATTTAAATG GTGATCATGT AACCCATCCT GACTTCATGT TGTATGACGC TCTTGATGTT GTTTTATACA TGGACCCAAT GTGCCTGGAT GCGTTCCCAA AATTAGTTTG TTTTAAAAAA CGTATTGAAG CTATCCCACA AATTGATAAG TACTTGAAAT CCAGCAAGTA TATAGCATGG CCTTTGCAGG GCTGGCAAGC CACGTTTGGT GGTGGCGACC ATCCTCCAAA ATCGGATCTG GTTCCGCGTG GATCCATGAT TGCCTCGCAT CTGCTTGCCT ACTTCTTCAC GGAGCTCAAC CATGACCAAG TGCAGAAGGT TGACCAGTAT CTCTACCACA TGCGCCTCTC TGATGAGACC CTCTTGGAGA TCTCTAAGCG GTTCCGCAAG GAGATGGAGA AAGGGCTTGG AGCCACCACT CACCCTACTG CAGCAGTGAA GATGCTGCCC ACCTTTGTGA GGTCCACTCC AGATGGGACA GAACACGGAG AGTTCCTGGC TCTGGATCTT GGAGGGACCA ACTTCCGTGT GCTTTGGGTG AAAGTAACGG ACAATGGGCT CCAGAAGGTG GAGATGGAGA ATCAGATCTA TGCCATCCCT GAGGACATCA TGCGAGGCAG TGGCACCCAG CTGTTTGACC ACATTGCCGA ATGCCTGGCT AACTTCATGG ATAAGCTACA AATCAAAGAC AAGAAGCTCC CACTGGGTTT TACCTTCTCG TTCCCCTGCC ACCAGACTAA ACTAGACGAG AGTTTCCTGG TCTCATGGAC CAAGGGATTC AAGTCCAGTG GAGTGGAAGG CAGAGACGTT GTGGCTCTGA TCCGGAAGGC CATCCAGAGG AGAGGGGACT TTGATATCGA CATTGTGGCT GTGGTGAATG ACACAGTTGG GACCATGATG ACCTGTGGTT ATGATGACCA CAACTGTGAG ATTGGTCTCA TTGTGGGCAC GGGCAGCAAC GCCTGCTACA TGGAAGAGAT GCGCCACATC GACATGGTGG AAGGCGATGA GGGGCGGATG TGTATCAATA TGGAGTGGGG GGCCTTCGGG GACGATGGCT CGCTCAACGA CATTCGCACT GAGTTTGACC AGGAGATTGA CATGGGCTCA CTGAACCCGG GAAAGCAACT GTTTGAGAAG ATGATCAGTG GGATGTACAT GGGGGAGCTG GTGAGGCTTA TCCTGGTGAA GATGGCCAAG GAGGAGCTGC TCTTTGGGGG GAAGCTCAGC CCAGAGCTTC TCAACACCGG TCGCTTTGAG ACCAAAGACA TCTCAGACAT TGAAGGGGAG AAGGATGGCA TCCGGAAGGC CCGTGAGGTC CTGATGCGGT TGGGCCTGGA CCCGACTCAG GAGGACTGCG TGGCCACTCA CCGGATCTGC CAGATCGTGT CCACACGCTC CGCCAGCCTG TGCGCAGCCA CCCTGGCCGC CGTGCTGCAG CGCATCAAGG AGAACAAAGG CGAGGAGCGG CTGCGCTCTA CTATTGGGGT CGACGGTTCC GTCTACAAGA AACACCCCCA TTTTGCCAAG CGTCTACATA AGACCGTGCG GCGGCTGGTG CCCGGCTGCG ATGTCCGCTT CCTCCGCTCC GAGGATGGCA GTGGCAAAGG TGCAGCCATG GTGACAGCAG TGGCTTACCG GCTGGCCGAT CAACACCGTG CCCGCCAGAA GACATTAGAG CATCTGCAGC TGAGCCATGA CCAGCTGCTG GAGGTCAAGA GGAGGATGAA GGTAGAAATG GAGCGAGGTC TGAGCAAGGA GACTCATGCC AGTGCCCCCG TCAAGATGCT GCCCACCTAC GTGTGTGCTA CCCCGGACGG CACAGAGAAA GGGGACTTCT TGGCCTTGGA CCTTGGAGGA ACAAATTTCC GGGTCCTGCT GGTCCGTGTT CGGAATGGGA AGTGGGGTGG AGTGGAGATG CACAACAAGA TCTACGCCAT CCCGCAGGAG GTCATGCACG GCACCGGGGA CGAGCTCTTT GACCACATTG TCCAGTGCAT CGCGGACTTC CTCGAGTACA TGGGCATGAA GGGCGTGTCC CTGCCTCTGG GTTTTACCTT CTCCTTCCCC TGCCAGCAGA ACAGCCTGGA CGAGAGCATC CTCCTCAAGT GGACAAAAGG CTTCAAGGCA TCTGGCTGCG AGGGCGAGGA CGTGGTGACC CTGCTGAAGG AAGCGATCCA CCGGCGAGAG GAGTTTGACC TGGATGTGGT TGCTGTGGTG AACGACACAG TCGGAACTAT GATGACCTGT GGCTTTGAAG ACCCTCACTG TGAAGTTGGC CTCATTGTTG GCACGGGCAG CAATGCCTGC TACATGGAGG AGATGCGCAA CGTGGAACTG GTGGAAGGAG AAGAGGGGCG GATGTGTGTG AACATGGAAT GGGGGGCCTT CGGGGACAAT GGATGCCTAG ATGACTTCCG CACAGAATTT GATGTGGCTG TGGATGAGCT TTCACTCAAC CCCGGCAAGC AGAGGTTCGA GAAAATGATC AGTGGAATGT ACCTGGGTGA GATTGTCCGT AACATTCTCA TCGATTTCAC CAAGCGTGGA CTACTCTTCC GAGGCCGCAT CTCAGAGCGG CTCAAGACAA GGGGCATCTT TGAAACCAAG TTCTTGTCTC AGATTGAGAG TGACTGCCTG GCCCTGCTGC AAGTCCGAGC CATCCTGCAA CACTTAGGGC TTGAGAGCAC CTGTGACGAC AGCATCATTG TTAAGGAGGT GTGCACTGTG GTGGCCCGGC GGGCAGCCCA GCTCTGTGGC GCAGGCATGG CCGCTGTGGT GGACAGGATA CGAGAAAACC GTGGGCTGGA CGCTCTCAAA GTGACAGTGG GTGTGGATGG GACCCTCTAC AAGCTACATC CTCACTTTGC CAAAGTCATG CATGAGACAG TGAAGGACCT GGCTCCGAAA TGTGATGTGT CTTTCCTGCA GTCAGAGGAT GGCAGCGGGA AGGGGGCGGC GCTCATCACT GCTGTGGCCT GCCGCATCCG TGAGGCTGGA CAGCGATAGC TCGAGCGGCC GCATCGTGAC TGACTGACGA TCTGCCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCGCAGCCA TGACCCAGTC ACGTAGCGAT AGCGGAGTGT ATAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAA ATTCCGACAC CATCGAATGG TGCAAAACCT TTCGCGGTAT GGCATGATAG CGCCCGGAAG AGAGTCAATT CAGGGTGGTG AATGTGAAAC CAGTAACGTT ATACGATGTC GCAGAGTATG CCGGTGTCTC TTATCAGACC GTTTCCCGCG TGGTGAACCA GGCCAGCCAC GTTTCTGCGA AAACGCGGGA AAAAGTGGAA GCGGCGATGG CGGAGCTGAA TTACATTCCC AACCGCGTGG CACAACAACT GGCGGGCAAA CAGTCGTTGC TGATTGGCGT TGCCACCTCC AGTCTGGCCC TGCACGCGCC GTCGCAAATT GTCGCGGCGA TTAAATCTCG CGCCGATCAA CTGGGTGCCA GCGTGGTGGT GTCGATGGTA GAACGAAGCG GCGTCGAAGC CTGTAAAGCG GCGGTGCACA ATCTTCTCGC GCAACGCGTC AGTGGGCTGA TCATTAACTA TCCGCTGGAT GACCAGGATG CCATTGCTGT GGAAGCTGCC TGCACTAATG TTCCGGCGTT ATTTCTTGAT GTCTCTGACC AGACACCCAT CAACAGTATT ATTTTCTCCC ATGAAGACGG TACGCGACTG GGCGTGGAGC ATCTGGTCGC ATTGGGTCAC CAGCAAATCG CGCTGTTAGC GGGCCCATTA AGTTCTGTCT CGGCGCGTCT GCGTCTGGCT GGCTGGCATA AATATCTCAC TCGCAATCAA ATTCAGCCGA TAGCGGAACG GGAAGGCGAC TGGAGTGCCA TGTCCGGTTT TCAACAAACC ATGCAAATGC TGAATGAGGG CATCGTTCCC ACTGCGATGC TGGTTGCCAA CGATCAGATG GCGCTGGGCG CAATGCGCGC CATTACCGAG TCCGGGCTGC GCGTTGGTGC GGATATCTCG GTAGTGGGAT ACGACGATAC CGAAGACAGC TCATGTTATA TCCCGCCGTT AACCACCATC AAACAGGATT TTCGCCTGCT GGGGCAAACC AGCGTGGACC GCTTGCTGCA ACTCTCTCAG GGCCAGGCGG TGAAGGGCAA TCAGCTGTTG CCCGTCTCAC TGGTGAAAAG AAAAACCACC CTGGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGGATTCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGCGCTT TGCCTGGTTT CCGGCACCAG AAGCGGTGCC GGAAAGCTGG CTGGAGTGCG ATCTTCCTGA GGCCGATACT GTCGTCGTCC CCTCAAACTG GCAGATGCAC GGTTACGATG CGCCCATCTA CACCAACGTA ACCTATCCCA TTACGGTCAA TCCGCCGTTT GTTCCCACGG AGAATCCGAC GGGTTGTTAC TCGCTCACAT TTAATGTTGA TGAAAGCTGG CTACAGGAAG GCCAGACGCG AATTATTTTT GATGGCGTTG GAATT
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
