pCMV-CNTNAP3(human)-mCherry-Neo
$99.00
In stock
SKU
PL28995
NM_033655.5;CASPR3; CNTNAP3A
100μg liquid plasmid(Instantly):$120
| name | pCMV-CNTNAP3(human)-mCherry-Neo | another name | apCMV-CNTNAP3(human)-mCherry-Neo |
| Insert Size (bp) | 3867bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | Red |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | pCMV-AMN(human)-mCherry-Neo | 5′ sequencing primer | CMV-F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGACTCAG ATCTCGAGCT CAAGCTTCGA ATTCGCCACC ATGGCTTCAG TGGCCTGGGC CGTCCTCAAG GTGCTGCTGC TTCTCCCCAC TCAGACTTGG AGCCCCGTGG GAGCAGGAAA TCCACCTGAC TGTGATGCCC CACTGGCCTC TGCCTTGCCT AGGTCATCCT TCAGCAGCTC CTCAGAGCTG TCCAGCAGCC ACGGCCCGGG GTTTTCAAGG CTTAATCGAA GAGATGGAGC TGGTGGCTGG ACTCCACTTG TGTCAAATAA ATACCAATGG CTGCAAATTG ACCTTGGAGA GAGAATGGAG GTCACTGCTG TCGCCACCCA AGGAGGATAT GGGAGCTCTG ACTGGGTGAC CAGCTACCTC CTGATGTTCA GTGATGGTGG GAGAAACTGG AAGCAGTATC GCCGAGAAGA AAGCATCTGG GGTTTTCCAG GAAACACAAA CGCAGACAGT GTGGTGCACT ACAGACTCCA GCCTCCCTTT GAAGCCAGGT TCCTGCGCTT TCTCCCTTTA GCCTGGAACC CTAGGGGCAG GATTGGGATG CGGATCGAAG TGTACGGATG TGCATATAAA TCTGAGGTGG TTTATTTTGA TGGACAAAGT GCTCTGCTGT ATAGACTTGA TAAAAAACCT TTAAAACCAA TAAGAGACGT TATTTCTTTG AAATTTAAAG CCATGCAGAG CAATGGAATT CTACTTCACA GAGAAGGACA ACATGGAAAT CACATTACTC TGGAATTAAT TAAAGGAAAG CTTGTCTTTT TTCTTAATTC AGGCAATGCT AAGCTGCCTT CCACTATTGC TCCTGTGACC CTCACCCTGG GCAGCCTGCT GGACGACCAG CACTGGCATT CCGTCCTCAT CGAGCTCCTC GACACGCAGG TCAACTTCAC CGTGGACAAA CACACTCATC ATTTCCAAGC AAAGGGAGAT TCCAGTTACT TGGATCTTAA TTTTGAGATC AGCTTTGGGG GAATTCCGAC ACCCGGAAGA TCGCGGGCAT TCAGACGTAA AAGCTTTCAT GGGTGTTTAG AAAATCTTTA TTATAATGGA GTGGATGTTA CCGAATTAGC CAAGAAACAC AAACCACAGA TCCTCATGAT GGGAAATGTG TCCTTCTCAT GTCCACAGCC ACAGACTGTC CCTGTGACTT TTCTGAGCTC CAGGAGTTAT CTGGCTCTGC CAGGCAACTC TGGGGAGGAC AAAGTGTCTG TCACTTTTCA ATTTCGAACG TGGAACAGAG CAGGACATTT GCTTTTCGGC GAACTTCGAC GTGGTTCAGG GAGTTTCGTC CTCTTTCTTA AGGATGGCAA GCTCAAACTG AGTCTCTTCC AGCCGGGACA GTCACCAAGG AATGTCACAG CAGGTGCTGG ATTAAACGAT GGGCAGTGGC ACTCTGTGTC CTTCTCTGCC AAGTGGAGCC ATATGAATGT GGTGGTGGAC GATGACACAG CTGTTCAGCC CCTGGTGGCT GTGCTCATTG ATTCAGGTGA CACCTATTAT TTTGGAGGCT GCCTGGACAA CAGCTCTGGC TCTGGATGTA AAAGCCCCCT GGGAGGGTTT CAGGGCTGCC TAAGGCTCAT CACCATTGGT GACAAAGCGG TGGATCCCAT CTTAGTACAG CAGGGGGCGC TGGGGAGTTT CAGGGACCTC CAGATAGACT CCTGCGGCAT CACAGACAGG TGCTTGCCCA GCTACTGTGA GCATGGGGGC GAGTGTTCCC AGTCGTGGGA CACCTTCTCC TGTGACTGTC TAGGCACAGG CTATACGGGC GAGACCTGCC ATTCCTCTCT CTACGAGCAG TCTTGTGAAG CCCACAAGCA CCGAGGGAAC CCGTCTGGGC TTTACTATAT TGATGCAGAT GGAAGTGGCC CCCTGGGACC ATTTCTTGTG TACTGCAATA TGACAGCAGA CGCCGCGTGG ACGGTGGTGC AGCACGGTGG CCCCGACGCG GTGACCCTCC GAGGTGCCCC CAGCGGGCAC CCGCGCTCGG CTGTGTCCTT CGCGTACGCA GCGGGCGCGG GGCAGCTGCG GTCCGCGGTG AACCTGGCGG AGCGCTGCGA GCAGCGGCTG GCTCTGCGCT GCGGGACGGC GCGGCGCCCG GACTCACGAG ATGGAACCCC ACTGAGCTGG TGGGTTGGAA GAACCAATGA AACACACACT TCCTGGGGAG GTTCTCTGCC TGATGCTCAA AAGTGTACTT GTGGATTAGA GGGGAACTGC ATTGATTCTC AGTATTACTG CAACTGTGAT GCTGGCCGGA ATGAATGGAC TAGTGACACA ATAGTCCTTT CCCAAAAGGA GCACCTGCCA GTCACTCAGA TTGTGATGAC AGACGCAGGC CGACCACATT CCGAAGCAGC TTATACACTG GGGCCACTGC TCTGCCGCGG AGATCAGTCA TTCTGGAATT CAGCTTCCTT CAACACTGAG ACTTCATACC TTCATTTCCC TGCTTTCCAC GGAGAACTCA CTGCTGACGT GTGCTTCTTT TTTAAGACCA CAGTTTCCTC CGGGGTGTTT ATGGAGAACC TGGGGATCAC AGATTTCATC AGGATTGAGC TGCGTGCTCC CACAGAAGTG ACCTTTTCCT TCGATGTGGG GAATGGACCT TGTGAGGTCA CGGTGCAGTC ACCCACTCCC TTTAATGACA ATCAGTGGCA CCACGTGAGG GCAGAGAGAA ATGTTAAAGG AGCGTCTCTT CAAGTTGATC AGCTTCCTCA GAAGATGCAG CCTGCCCCTG CTGATGGGCA CGTTCGTTTA CAGCTCAACA GCCAGCTCTT CATTGGTGGA ACGGCCACCA GACAGAGAGG CTTTCTAGGA TGCATTCGGT CTCTGCAGTT GAACGGGGTG GCCCTGGATC TGGAAGAAAG AGCCACAGTG ACGCCAGGAG TGGAGCCAGG GTGTGCAGGA CACTGCAGCA CCTATGGACA CTTGTGTCGC AATGGAGGGA GATGCAGAGA GAAACGCAGG GGGGTCACCT GTGACTGTGC CTTCTCAGCC TATGATGGGC CGTTCTGCTC CAATGAGATT TCCGCATATT TTGCAACTGG CTCCTCAATG ACATACCATT TTCAAGAACA TTACACTTTA AGTGAAAACT CCAGCTCTCT CGTTTCTTCA TTACACAGAG ATGTAACATT GACCAGAGAA ATGATCACAC TGAGCTTCCG AACCACACGA ACTCCGAGCT TATTGCTGTA TGTGAGCTCT TTCTATGAGG AATACCTTTC AGTTATCCTC GCCAACAATG GAAGTTTGCA GATTAGGTAC AAGCTAGATA GACATCAAAA TCCTGATGCA TTTACCTTTG ATTTTAAAAA CATGGCTGAT GGGCAACTTC ACCAAGTGAA GATTAACAGA GAAGAAGCTG TGGTCATGGT AGAGGTTAAC CAGAGCACAA AGAAACAAGT CATCTTGTCC TCAGGGACAG AATTCAACGC CGTCAAATCT CTCATATTGG GAAAGGTTTT AGAGGCTGCC GGCGCGGACC CGGACACAAG GCGGGCGGCG ACTAGTGGCT TCACTGGCTG CCTCTCGGCG GTGCGCTTCG GCCGCGCTGC TCCCCTGAAG GCGGCGCTGC GCCCCAGCGG CCCCTCCCGG GTCACCGTCC GCGGCCACGT GGCCCCTATG GCCCGCTGCG CAGCGGGGGC GGCGTCCGGC TCCCCGGCGC GGGAACTGGC TCCCCGACTC GCGGGGGGCG CAGGTCGTTC TGGACCAGCG GATGAGGGAG AGCCCTTGGT TAATGCAGAC AGAAGAGACT CTGCTGTCAT CGGAGGTGTG ATAGCAGTGG TGATATTTAT TTTGCTTTGC ATCACTGCCA TAGCCATACG CATCTATCAA CAGAGAAAGT TACGCAAAGA AAATGAGTCA AAAGTCTCAA AAAAAGAAGA GTGCGATATC GGCGGTGGAG GAAGCGGCGG AGGTGGATCT ATGGATCCAC CGGTCGCCAC CATGGTGAGC AAGGGCGAGG AGGATAACAT GGCCATCATC AAGGAGTTCA TGCGCTTCAA GGTGCACATG GAGGGCTCCG TGAACGGCCA CGAGTTCGAG ATCGAGGGCG AGGGCGAGGG CCGCCCCTAC GAGGGCACCC AGACCGCCAA GCTGAAGGTG ACCAAGGGTG GCCCCCTGCC CTTCGCCTGG GACATCCTGT CCCCTCAGTT CATGTACGGC TCCAAGGCCT ACGTGAAGCA CCCCGCCGAC ATCCCCGACT ACTTGAAGCT GTCCTTCCCC GAGGGCTTCA AGTGGGAGCG CGTGATGAAC TTCGAGGACG GCGGCGTGGT GACCGTGACC CAGGACTCCT CCCTGCAGGA CGGCGAGTTC ATCTACAAGG TGAAGCTGCG CGGCACCAAC TTCCCCTCCG ACGGCCCCGT AATGCAGAAG AAGACCATGG GCTGGGAGGC CTCCTCCGAG CGGATGTACC CCGAGGACGG CGCCCTGAAG GGCGAGATCA AGCAGAGGCT GAAGCTGAAG GACGGCGGCC ACTACGACGC TGAGGTCAAG ACCACCTACA AGGCCAAGAA GCCCGTGCAG CTGCCCGGCG CCTACAACGT CAACATCAAG TTGGACATCA CCTCCCACAA CGAGGACTAC ACCATCGTGG AACAGTACGA ACGCGCCGAG GGCCGCCACT CCACCGGCGG CATGGACGAG CTGTACAAGT AGCGGCCGCG ACTCTAGATC ATAATCAGCC ATACCACATT TGTAGAGGTT TTACTTGCTT TAAAAAACCT CCCACACCTC CCCCTGAACC TGAAACATAA AATGAATGCA ATTGTTGTTG TTAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTAAGGCGT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTCCT GAGGCGGAAA GAACCAGCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAGATC GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAAGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGAG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCTAG GGGGAGGCTA ACTGAAACAC GGAAGGAGAC AATACCGGAA GGAACCCGCG CTATGACGGC AATAAAAAGA CAGAATAAAA CGCACGGTGT TGGGTCGTTT GTTCATAAAC GCGGGGTTCG GTCCCAGGGC TGGCACTCTG TCGATACCCC ACCGAGACCC CATTGGGGCC AATACGCCCG CGTTTCTTCC TTTTCCCCAC CCCACCCCCC AAGTTCGGGT GAAGGCCCAG GGCTCGCAGC CAACGTCGGG GCGGCAGGCC CTGCCATAGC CTCAGGTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCA TGCAT
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