pAAV-ITR-U6-sgRNA(backbone)-DIO2(human)-Cre-hGHpA-ITR
$55.00
In stock
SKU
PL28988
SC82233
100μg liquid plasmid(Instantly):$76
| name | pAAV-ITR-U6-sgRNA(backbone)-DIO2(human)-Cre-hGHpA-ITR | another name | apAAV-ITR-U6-sgRNA(backbone)-DIO2(human)-Cre-hGHpA-ITR |
| Insert Size (bp) | Plasmid host | Mammary cell,Adeno-associated virus | |
| Plasmid uses | Gene editing | Fragment type | CRISPR,Cre |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | U6 | Replicon | |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR | 5′ sequencing primer | G36004-F3 |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT AGGGGTTCCT GCGGCCGCAC GCGTGAGGGC CTATTTCCCA TGATTCCTTC ATATTTGCAT ATACGATACA AGGCTGTTAG AGAGATAATT GGAATTAATT TGACTGTAAA CACAAAGATA TTAGTACAAA ATACGTGACG TAGAAAGTAA TAATTTCTTG GGTAGTTTGC AGTTTTAAAA TTATGTTTTA AAATGGACTA TCATATGCTT ACCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAAC ACCGGAAGAG CGAGCTCTTC TGTTTTAGAG CTAGAAATAG CAAGTTAAAA TAAGGCTAGT CCGTTATCAA CTTGAAAAAG TGGCACCGAG TCGGTGCTTT TTTGTTTTAG AGCTAGAAAT AGCAAGTTAA AATAAGGCTA GTCCGTAGCG CGTGCGCCAA TTCTGCAGAC AAATTCTAGA GGTACCTAAA GTGGGGGTCA TGCCATTCTG CCAAAGCATT GTCTGCCTAA ATACCTGTCC TAAAGACTCA TGGGAGAGGC AAGCCAATGA ACAATGGTCA ATGTGGCACC TTGAACCAAT TCAGTCGGCT CTTAACTTCC CATACATTTA ATTAAAATTT TTTTCAAACA AAAAGGAAGA TTCCTTAAAG TATCAGGTCT CTTAGGGGTT ATGCTAACAG AAGCAGGTGA AACACCAAAT TTAAAGAGCT TTCATTAATA ATTTCTCAGC CTTATGGCTT CTTAATGCTA TTCAATGGAA AGTTTTAATT TTATCTGCAA AATGAAGCTA AAAGAATGTC GCCTAGCTCC TTCCCTGTCT GGAGGAACTT GGATTTTTTT TTTTTTTTTC ATTAGAAGCT TGGGTAAAAC TAAAAAGAAA CCTGTATTCA AGTTTCTGCA AGAAGCTTTA AAGAAATGCT GGGATGGTAC TCAAATAGTT TACAAGTTTT CCACCTACAA CTCATTTTGC ATTTTCTTAA ATAAGATAAT GACTCAAAAA AATTTAAAGG ACCTAGGTCA CAGATCTTAC AAAGGGTTGG AGTCATGGTA TAAAGGTATA AAGATTGTAT ATAAATGTTC ATATTTTCCA AGAAACAGAG CAAAGACCGC CTGTGATAAC TCTTCCTTTT TTCTACCTGC CTTCCAGGAT CCTTTCTTTC CAAAACCCCT CATAAATTAA TGATAATGAT AATCAGAAGA GAGAGTGTTG GCCATATCAA TCTGCACATT ATATTAAATT ACTTTTCCCA AAGTTTGGAA GCATTTCCTG AAGGCTGTCA AGGGTATTAG TTTTTTTCTC TCTTTAAATC ACAAAGCAAA ATTCCACTGT CTCTCCCCAA CCCCTTCTAA AAAAAAAAAG TTGTCGAGTT TTGCTTTTCT ATTCACTGCA ATCCTGGCCA AAGTAAAGCC CTCTTTCTCA ATGACGTCAA GATCTTTACC AAGATTAGGC TTTCACTTCT CTATTGCAGC AATTAGCCAG GGAATGTATA AAAGGCGTCA GGGAGACTCA CTGGGCTGCA GAGAGAGGCA CTTTGCACCA CAGACAGATA GCAAGAAGGG AAAGACAGAG AGTGAGAAAA AAGAGGAGTC AGTCGCTCCT GGGGAAGGGA GAGAGTGAGA CTGGGAGAAA GAGAAGCACA GAAAGTGTGT GTAAAACGGA GTAAAGAAAG AAAAAAAAAA AACTACCCTT AAAGCACATT TAAAAAAAAA AAAAACTCTG GCAATTCAAG AAAGAAACAG GCTACGTTTA AAGAGCATAG AGACAATGAA AGGCTAAAGA AAATTTTAAA ATCTCTGCCA CAGTCTCATA GGTGCTTGGA AATGAAAGTA GAACTGCCTG TCTTTAACGG ACTCTGACAG AGGTAACTGG ATTAGGGACG AGTACGCCAG CTTTTTTTTT TTTTTTTTTT TTTTTTTTTT AACATCTTAA ATCCTGAAAA AAAAAAAAAA AAAAAAAAAA AGGCAGCAGC TCCGAATTGA ATGAATTGAT GGGCACACTC CAACTGCTGG GCTGGAGAGA CTGGACTTAG TCTTGCCATT TCTGCTTCTT TGAAAGAGGA GACAACTTGG GCTTCCTTTT AATTTAGTTT TTTTTCCCCT TCTCCCCCAA CCCCCAACCT TCCCCCTTAC CTCCCCCACC CCCTTTATCA CCACCCCCCT TTTAAATAAG AGGGTGAAGG GGAACCAGAG CGCACAAGGG AACTGACTCA GGAGGCAGAG AAGATGGGCA TCCTCAGCGT AGACTTGCTG ATCACACTGC AAATTCTGCC AGTTTTTTTC TCCAACTGCC TCTTCCTGGC TCTCTATGAC TCGGTCATTC TGCTCAAGCA CGTGGTGCTG CTGTTGAGCC GCTCCAAGTC CACTCGCGGA GAGTGGCGGC ACCGGTGCCA CCATGCCCAA GAAGAAGAGG AAGGTGTCCA ATCTCCTGAC TGTTCACCAG AACCTCCCTG CGCTGCCAGT AGATGCCACT AGCGATGAGG TCAGGAAAAA TCTCATGGAT ATGTTTAGGG ATAGACAGGC GTTTTCTGAA CACACCTGGA AAATGCTGCT TAGCGTGTGC CGATCCTGGG CAGCCTGGTG TAAGCTGAAC AATCGCAAAT GGTTCCCCGC CGAGCCGGAG GACGTGCGCG ATTACCTGCT GTATCTCCAG GCAAGAGGGC TGGCTGTCAA GACTATCCAG CAGCACTTGG GCCAACTGAA TATGCTGCAT CGACGCAGCG GGCTCCCCCG GCCTAGCGAT TCAAACGCAG TCTCCCTTGT TATGAGGAGA ATTAGAAAGG AAAACGTAGA TGCGGGTGAG AGGGCTAAGC AGGCTCTCGC TTTTGAGCGG ACTGATTTCG ACCAGGTCAG ATCCCTGATG GAGAACAGCG ATCGGTGCCA GGACATCAGG AACCTCGCAT TTCTGGGAAT TGCATATAAC ACACTTCTGC GCATAGCTGA GATCGCCCGG ATCAGAGTGA AAGACATCAG TCGAACGGAC GGCGGCCGGA TGCTTATTCA TATTGGACGC ACAAAGACAT TGGTCAGCAC CGCTGGCGTT GAAAAGGCCT TGTCCCTGGG CGTAACGAAG CTGGTGGAAA GATGGATCTC AGTGTCCGGC GTGGCTGACG ACCCTAATAA TTACTTGTTC TGTCGAGTGA GAAAAAACGG AGTCGCCGCG CCCTCTGCCA CCAGCCAATT GAGTACACGG GCCCTTGAAG GGATCTTTGA GGCAACCCAC CGACTCATAT ACGGAGCCAA GGATGACAGT GGCCAGAGGT ATCTCGCCTG GTCAGGTCAT TCTGCTAGGG TGGGGGCCGC ACGAGACATG GCGCGGGCAG GAGTCTCCAT ACCAGAGATT ATGCAAGCTG GAGGTTGGAC AAATGTGAAC ATCGTTATGA ACTATATCCG CAATCTTGAC TCTGAAACCG GGGCCATGGT GAGACTGCTC GAAGATGGTG ACTACCCATA CGATGTTCCA GATTACGCTT AAGAATTCGA TATCAAGCTT ATCGATAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGAACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AATCATCGTC CTTTCCTTGG CTGCTCGCCT ATGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCATCGA TACCGAGCGC TGCTCGAGAG ATCTACGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TGTAGGTAAC CACGTGCGGA CCGAGCGGCC GCAGGAACCC CTAGTGATGG AGTTGGCCAC TCCCTCTCTG CGCGCTCGCT CGCTCACTGA GGCCGGGCGA CCAAAGGTCG CCCGACGCCC GGGCTTTGCC CGGGCGGCCT CAGTGAGCGA GCGAGCGCGC AGCTGCCTGC AGGGGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAC GTCAAAGCAA CCATAGTACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCTTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTTGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACT CTATCTCGGG CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGTC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA ACGTTTACAA TTTTATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT
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