pEX18Tc-FliH(PAO1)
$99.00
In stock
SKU
PL28769
QPV56742.1
100μg liquid plasmid(Instantly):$120
| name | pEX18Tc-FliH(PAO1) | another name | apEX18Tc-FliH(PAO1) |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene editing | Fragment type | |
| Fragment species | Prokaryotic resistance | Tet | |
| Selective marker | SacB | Fluorescent labeling | |
| Promoter | Replicon | pBR322 | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pEX18TC | 5′ sequencing primer | PUC57-F1 |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACCGATTAG CTTTAATGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGGCACC GTGTATGAAA TCTAACAATG CGCTCATCGT CATCCTCGGC ACCGTCACCC TGGATGCTGT AGGCATAGGC TTGGTTATGC CGGTACTGCC GGGCCTCTTG CGGGATATCG TCCATTCCGA CAGCATCGCC AGTCACTATG GCGTGCTGCT AGCGCTATAT GCGTTGATGC AATTTCTATG CGCACCCGTT CTCGGAGCAC TGTCCGACCG CTTTGGCCGC CGCCCAGTCC TGCTCGCTTC GCTACTTGGA GCCACTATCG ACTACGCGAT CATGGCGACC ACACCCGTCC TGTGGATTCT CTACGCCGGA CGCATCGTGG CCGGCATCAC CGGCGCCACA GGTGCGGTTG CTGGCGCCTA TATCGCCGAC ATCACCGATG GGGAAGATCG GGCTCGCCAC TTCGGGCTCA TGAGCGCTTG TTTCGGCGTG GGTATGGTGG CAGGCCCCGT GGCCGGGGGA CTGTTGGGCG CCATCTCCTT ACATGCACCA TTCCTTGCGG CGGCGGTGCT CAACGGCCTC AACCTACTAC TGGGCTGCTT CCTAATGCAG GAGTCGCATA AGGGAGAGCG CCGACCGATG CCCTTGAGAG CCTTCAACCC AGTCAGCTCC TTCCGGTGGG CGCGGGGCAT GACTATCGTC GCCGCACTTA TGACTGTCTT CTTTATCATG CAACTCGTAG GACAGGTGCC GGCAGCGCTC TGGGTCATTT TCGGCGAGGA CCGCTTTCGC TGGAGCGCGA CGATGATCGG CCTGTCGCTT GCGGTATTCG GAATCTTGCA CGCCCTCGCT CAAGCCTTCG TCACTGGTCC CGCCACCAAA CGTTTCGGCG AGAAGCAGGC CATTATCGCC GGCATGGCGG CCGACGCGCT GGGCTACGTC TTGCTGGCGT TCGCGACGCG AGGCTGGATG GCCTTCCCCA TTATGATTCT TCTCGCTTCC GGCGGCATCG GGATGCCCGC GTTGCAGGCC ATGCTGTCCA GGCAGGTAGA TGACGACCAT CAGGGACAGC TTCAAGGATC GCTCGCGGCT CTTACCAGCC TAACTTCGAT CACTGGACCG CTGATCGTCA CGGCGATTTA TGCCGCCTCG GCGAGCACAT GGAACGGGTT GGCATGGATT GTAGGCGCCG CCCTATACCT TGTCTGCCTC CCCGCGTTGC GTCGCGGTGC ATGGAGCCGG GCCACCTCGA CCTGAATGGA AGCCGGCGGC ACCTCGCTAA CGGATTCACC ACTCCAAGAA TTGGAGCCAA TCAATTCTTG CGGAGAACTG TGAATGCGCA AACCAACCCT TGCATCGATT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGA ATTCGTGGTC CCCCACGACA AGGACAAAGA CAATCCCAGC GAACTGATCC GCGGCAAGGA CGTGGCGGCC TTCGGCCTCT GGTCGTTGCC GAGCTTCGAC GAGCCGCGCG ACGAGCCGGC CGTTGCCGCG CCGCAGGTGC CCGCGGTCGC CGAACCCGCG CCGGCGCCGC CGGCAGTGGA AGAGGTCGAG CTGGAGACCG TCAAGCCGCC GACGCTGGAG GAGATCGAGG CGATCCGCCA GGACGCCTAC AACGAAGGCT TCGCCACCGG CGAGCGCGAC GGTTTCCACG CCGGCCAGTT GAAGGCGCGG CAGGAAGCCG AGGAGGCGCT GAAGGAGCGT CTGCAAAGTC TCGAGCGTCT GATGACGCAG CTTCTGGAAC CCATCGCCGA GCAGGACGCC CTGATCGAGC AGGGCATGGT CAACCTGGTC AACCACGTTG CCCGCCAAGT GATCCAGCGC GAACTGCACA TGGACTCCAG CCATGTCCGC CAGGTCCTGC GCGAGGCTCT GAAGCTGCTG CCGATGGGCG CGGCGAACAT CCGTATCCAT GTCAACCCGC AGGACTTCGA GCGGGTCAAG GCGCTGCGCG AGCGCCACGA AGAGAGCTGG CGGATTCTCG AGGACGATTC GTTGCTGCCC GGCGGCTGCC GCATCGAGAC CGAGCACAGT CGCATCGACG CGACCATCGA GACGCGCCTG GCGCAGGCAG TGAAGCAACT GTTCGAGCAA CAGCGCGAAC AGGCGACCCA TCCGCTGGCC AGCGACATCC GGATCGACCT CGACGCCCCC GGAGACGTCG ATGCGCCTTG AGGATCCTCT AGAGTCGACC TGCAGGCATG CAAGCTTGGC ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGCGA GCTTGGCTGT TTTGGCGGAT GAGAGAAGAT TTTCAGCCTG ATACAGATTA AATCAGAACG CAGAAGCGGT CTGATAAAAC AGAATTTGCC TGGCGGCAGT AGCGCGGTGG TCCCACCTGA CCCCATGCCG AACTCAGAAG TGAAACGCCG TAGCGCCGAT GGTAGTGTGG GGTCTCCCCA TGCGAGAGTA GGGAACTGCC AGGCATCAAA TAAAACGAAA GGCTCAGTCG AAAGACTGGG CCTTTCGTTT TATCTGTTGT TTGTCGGTGA ACGCTCTCCT GAGTAGGACA AATCCGCCGG GAGCGGATTT GAACGTTGCG AAGCAACGGC CCGGAGGGTG GCGGGCAGGA CGCCCGCCAT AAACTGCCAG GCATCAAATT AAGCAGAAGG CCATCCTGAC GGATGGCCTT TTTGCGTTTC TACAAACTCT TTTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGCGCCTGA TGCGGTATTT TCTCCTTACG CATATCGACA TCCGCCCTCA CCGCCAGGAA CGCAACCGCA GCCTCATCAC GCCGGCGCTT CTTGGCCGCG CGGGATTCAA CCCACTCGGC CAGCTCGTCG GTGTAGCTCT TTGGCATCGT CTCTCGCCTG TCCCCTCAGT TCAGTAATTT CCTGCATTTG CCTGTTTCCA GTCGGTAGAT ATTCCACAAA ACAGCAGGGA AGCAGCGCTT TTCCGCTGCA TAACCCTGCT TCGGGGTCAT TATAGCGATT TTTTCGGTAT ATCCATCCTT TTTCGCACGA TATACAGGAT TTTGCCAAAG GGTTCGTGTA GACTTTCCTT GGTGTATCCA ACGGCGTCAG CCGGGCAGGA TAGGTGAAGT AGGCCCACCC GCGAGCGGGT GTTCCTTCTT CACTGTCCCT TATTCGCACC TGGCGGTGCT CAACGGGAAT CCTGCTCTGC GAGGCTGGCC GGCTACCGCC GGCGTAACAG ATGAGGGCAA GCGGATGGCT GATGAAACCA AGCCAACCAG GAAGGGCAGC CCACCTATCA AGGTGTACTG CCTTCCAGAC GAACGAAGAG CGATTGAGGA AAAGGCGGCG GCGGCCGGCA TGAGCCTGTC GGCCTACCTG CTGGCCGTCG GCCAGGGCTA CAAAATCACG GGCGTCGTGG ACTATGAGCA CGTCCGCGAG CTGGCCCGCA TCAATGGCGA CCTGGGCCGC CTGGGCGGCC TGCTGAAACT CTGGCTCACC GACGACCCGC GCACGGCGCG GTTCGGTGAT GCCACGATCC TCGCCCTGCT GGCGAAGATC GACTCTAGCT AGAGGATCGA TCCTTTTTAA CCCATCACAT ATACCTGCCG TTCACTATTA TTTAGTGAAA TGAGATATTA TGATATTTTC TGAATTGTGA TTAAAAAGGC AACTTTATGC CCATGCAACA GAAACTATAA AAAATACAGA GAATGAAAAG AAACAGATAG ATTTTTTAGT TCTTTAGGCC CGTAGTCTGC AAATCCTTTT ATGATTTTCT ATCAAACAAA AGAGGAAAAT AGACCAGTTG CAATCCAAAC GAGAGTCTAA TAGAATGAGG TCGAAAAGTA AATCGCGCGG GTTTGTTACT GATAAAGCAG GCAAGACCTA AAATGTGTAA AGGGCAAAGT GTATACTTTG GCGTCACCCC TTACATATTT TAGGTCTTTT TTTATTGTGC GTAACTAACT TGCCATCTTC AAACAGGAGG GCTGGAAGAA GCAGACCGCT AACACAGTAC ATAAAAAAGG AGACATGAAC GATGAACATC AAAAAGTTTG CAAAACAAGC AACAGTATTA ACCTTTACTA CCGCACTGCT GGCAGGAGGC GCAACTCAAG CGTTTGCGAA AGAAACGAAC CAAAAGCCAT ATAAGGAAAC ATACGGCATT TCCCATATTA CACGCCATGA TATGCTGCAA ATCCCTGAAC AGCAAAAAAA TGAAAAATAT CAAGTTCCTG AGTTCGATTC GTCCACAATT AAAAATATCT CTTCTGCAAA AGGCCTGGAC GTTTGGGACA GCTGGCCATT ACAAAACGCT GACGGCACTG TCGCAAACTA TCACGGCTAC CACATCGTCT TTGCATTAGC CGGAGATCCT AAAAATGCGG ATGACACATC GATTTACATG TTCTATCAAA AAGTCGGCGA AACTTCTATT GACAGCTGGA AAAACGCTGG CCGCGTCTTT AAAGACAGCG ACAAATTCGA TGCAAATGAT TCTATCCTAA AAGACCAAAC ACAAGAATGG TCAGGTTCAG CCACATTTAC ATCTGACGGA AAAATCCGTT TATTCTACAC TGATTTCTCC GGTAAACATT ACGGCAAACA AACACTGACA ACTGCACAAG TTAACGTATC AGCATCAGAC AGCTCTTTGA ACATCAACGG TGTAGAGGAT TATAAATCAA TCTTTGACGG TGACGGAAAA ACGTATCAAA ATGTACAGCA GTTCATCGAT GAAGGCAACT ACAGCTCAGG CGACAACCAT ACGCTGAGAG ATCCTCACTA CGTAGAAGAT AAAGGCCACA AATACTTAGT ATTTGAAGCA AACACTGGAA CTGAAGATGG CTACCAAGGC GAAGAATCTT TATTTAACAA AGCATACTAT GGCAAAAGCA CATCATTCTT CCGTCAAGAA AGTCAAAAAC TTCTGCAAAG CGATAAAAAA CGCACGGCTG AGTTAGCAAA CGGCGCTCTC GGTATGATTG AGCTAAACGA TGATTACACA CTGAAAAAAG TGATGAAACC GCTGATTGCA TCTAACACAG TAACAGATGA AATTGAACGC GCGAACGTCT TTAAAATGAA CGGCAAATGG TATCTGTTCA CTGACTCCCG CGGATCAAAA ATGACGATTG ACGGCATTAC GTCTAACGAT ATTTACATGC TTGGTTATGT TTCTAATTCT TTAACTGGCC CATACAAGCC GCTGAACAAA ACTGGCCTTG TGTTAAAAAT GGATCTTGAT CCTAACGATG TAACCTTTAC TTACTCACAC TTCGCTGTAC CTCAAGCGAA AGGAAACAAT GTCGTGATTA CAAGCTATAT GACAAACAGA GGATTCTACG CAGACAAACA ATCAACGTTT GCGCCTAGCT TCCTGCTGAA CATCAAAGGC AAGAAAACAT CTGTTGTCAA AGACAGCATC CTTGAACAAG GACAATTAAC AGTTAACAAA TAAAAACGCA AAAGAAAATG CCGATTATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG A
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