pB4GALT1(human)-luc2
$70.00
In stock
SKU
PL28713
·
100μg liquid plasmid(Instantly):$91
| name | pB4GALT1(human)-luc2 | another name | apB4GALT1(human)-luc2 |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Promoter detection | Fragment type | Promoter |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Fluc | |
| Promoter | Replicon | ||
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pGL4.10[luc2] | 5′ sequencing primer | RVP3 |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGCCTAACTG GCCGGTACCT CTTTTGACCT CACAAGTCAA CTTCTAGGAA TTCATCCTAA GAAAACAAAG TCACACCCCA AGATGTATGT GCGTCAGTGT TCGCCCTAGT GCTGTTTGTA GCCCTGGAAA ATGAGAAGCC ACCGTCAACG GGGGACTGTG CTAAGTAAAA TGAAGTACAT CCAAACAATC AAGTACTATG CAACCATTGA GAATGATGAA GTGGATCTAT ATTTATCGAT TGGAAAGAGG CCTAAGGAAA CAAAGGAAAC TACAGAATGG TCTTTCCATA TGAGGCAGGG AAATGTATGA ATAATTTAGT GCAGAAATTA CTCTGTAATT GTTTCTGGGA GCAGATGCAT GAGCAAAAAA TTGAAGATGA TTTTCCTTCT CTACATAGCC TGGGGCCAGG CCCCTGTTCT AAGCGTCCCC GCCCCACCCT GGGCTGAGGC CTTTGTTCAG AATGTTGGCC CTGTTGAGAG GACATGCTGG GTTGTCTTCT GGGAGGAGTT GCATTCCCCA TAAGTCCCCC AGTCTTGGAA GGGTGATGCA GGGTTCCTCC CCTGGGGGAA TGGTGGAGGC CTGAACTCTG TTCACAGGTG GTGGGAAATG GCATGGCTGT AGGAAGGTCT GAGAAGGCAG AATGGGAGCT CAACACAGGC TGGGGGGGCT TCTGGCAATT CTCTCAGGTT TAGAAAAATA GTTACAACAT TTCTTGCCAC TCTTTTGTGA GGGCAATTGA ACTGTCAAGC CTCAAGACAC TAAAGAATGG AAAGAATGCG GTAGGAGAGG AACACTGTCT TGGCTCTGGA GGGGCGAGAT TCGCCTCTTG TGCCTGATCA GAGCTCGACA AGGGGGACCC CTTTTCTAGT AGATCTTTGA CTCCAGAATC TAGGAGCCCT GTTGCAGAGG GAGGCCCTGA CATCTGGATT TTGACACAGA CCTGAGCCTG GAGCTGTTGG AAACTCATTT CTGGGACAGA TGTGGAATGC TGGACAATCA AACGTCGCCG GGGCCTTTGG AGTGCTCCCT GTGTCCTGTT GCGGTGGCCA CAGTGACTTT GGTCTCCCCT GATGACAGAA GCACAGGAGA AAGGCCAGCT CCATGGCCCA GCTCTTAGTT CCCACCTTCC TCCAAGGCGG GCCTGTGGGG CAGGGAGGGT GCAGGTGGAC TCGTGAACCC CTCACCCGTG TTCACCCTCC ATGAGCACAC TCGGCGCTTT CCCACGTACC GTGTGTTGCT CCTTACAATA ATTCCAGCAT GGGAAACTTA GGCTCAGGGA GCTCACATGA CTTGCACAAG GTCACACAGA GTTTGGGTCT GACACAGACC TCCTGAGTCC CAGGCTAGGG CTCTGGCCAC TGGACCATGA CCCAGCTCCT CATCCACACA GTGTGGGAGC AGGGCGATGG AGGCTGTGAG GTTCCTTGCC CCAGCCAGGG AAGCTGTGGC CTCAGCAGCA CTGCCGAGAG AAGTACAGCC TGGATCCAGG GCTGGAGCCA AGAGCACCTT TGGGATGAGA TTGTTTGTGT TTTGTTTTGT TTTCCCTTTA CTGTTTTAGA GGACTTAGGA ACCTAGTAAA CTTGACAGCG ACAGTTCCGT ATACCAGGGG CATTGCATTG GTGTCACCAC GGTCTGGGAG ATGTACAGGA ATGGTTAACA CTGAAACAAT CCATGAGGAT GCAAGGAGGC CTGAGGCGTG GGGAAGAGGG AAGAAGGGAG ACTCCTGAGG GCTGGGGATG CAAGTGGACC TGAAGACATG GGGTTTTCTG CCTCTGAGGC ATCACTCTGG GCAGCAACAA CCAGTTCCTG CTTCATGGAC AACACAGCTT CTTAATCCTC CTGGACCTGC CCTGCCTGGG CCAACACCTG GTGCTCAGGA GGGCTCAGCA CAGTGTCTGT CTGAAGACTG CGGGGACCCA GAAGTCCAGG TGGAGACCTA GGATTCCCCA ATAAGCTTGG CAATCCGGTA CTGTTGGTAA AGCCACCATG GAAGATGCCA AAAACATTAA GAAGGGCCCA GCGCCATTCT ACCCACTCGA AGACGGGACC GCCGGCGAGC AGCTGCACAA AGCCATGAAG CGCTACGCCC TGGTGCCCGG CACCATCGCC TTTACCGACG CACATATCGA GGTGGACATT ACCTACGCCG AGTACTTCGA GATGAGCGTT CGGCTGGCAG AAGCTATGAA GCGCTATGGG CTGAATACAA ACCATCGGAT CGTGGTGTGC AGCGAGAATA GCTTGCAGTT CTTCATGCCC GTGTTGGGTG CCCTGTTCAT CGGTGTGGCT GTGGCCCCAG CTAACGACAT CTACAACGAG CGCGAGCTGC TGAACAGCAT GGGCATCAGC CAGCCCACCG TCGTATTCGT GAGCAAGAAA GGGCTGCAAA AGATCCTCAA CGTGCAAAAG AAGCTACCGA TCATACAAAA GATCATCATC ATGGATAGCA AGACCGACTA CCAGGGCTTC CAAAGCATGT ACACCTTCGT GACTTCCCAT TTGCCACCCG GCTTCAACGA GTACGACTTC GTGCCCGAGA GCTTCGACCG GGACAAAACC ATCGCCCTGA TCATGAACAG TAGTGGCAGT ACCGGATTGC CCAAGGGCGT AGCCCTACCG CACCGCACCG CTTGTGTCCG ATTCAGTCAT GCCCGCGACC CCATCTTCGG CAACCAGATC ATCCCCGACA CCGCTATCCT CAGCGTGGTG CCATTTCACC ACGGCTTCGG CATGTTCACC ACGCTGGGCT ACTTGATCTG CGGCTTTCGG GTCGTGCTCA TGTACCGCTT CGAGGAGGAG CTATTCTTGC GCAGCTTGCA AGACTATAAG ATTCAATCTG CCCTGCTGGT GCCCACACTA TTTAGCTTCT TCGCTAAGAG CACTCTCATC GACAAGTACG ACCTAAGCAA CTTGCACGAG ATCGCCAGCG GCGGGGCGCC GCTCAGCAAG GAGGTAGGTG AGGCCGTGGC CAAACGCTTC CACCTACCAG GCATCCGCCA GGGCTACGGC CTGACAGAAA CAACCAGCGC CATTCTGATC ACCCCCGAAG GGGACGACAA GCCTGGCGCA GTAGGCAAGG TGGTGCCCTT CTTCGAGGCT AAGGTGGTGG ACTTGGACAC CGGTAAGACA CTGGGTGTGA ACCAGCGCGG CGAGCTGTGC GTCCGTGGCC CCATGATCAT GAGCGGCTAC GTTAACAACC CCGAGGCTAC AAACGCTCTC ATCGACAAGG ACGGCTGGCT GCACAGCGGC GACATCGCCT ACTGGGACGA GGACGAGCAC TTCTTCATCG TGGACCGGCT GAAGAGCCTG ATCAAATACA AGGGCTACCA GGTAGCCCCA GCCGAACTGG AGAGCATCCT GCTGCAACAC CCCAACATCT TCGACGCCGG GGTCGCCGGC CTGCCCGACG ACGATGCCGG CGAGCTGCCC GCCGCAGTCG TCGTGCTGGA ACACGGTAAA ACCATGACCG AGAAGGAGAT CGTGGACTAT GTGGCCAGCC AGGTTACAAC CGCCAAGAAG CTGCGCGGTG GTGTTGTGTT CGTGGACGAG GTGCCTAAAG GACTGACCGG CAAGTTGGAC GCCCGCAAGA TCCGCGAGAT TCTCATTAAG GCCAAGAAGG GCGGCAAGAT CGCCGTGTAA TAATTCTAGA GTCGGGGCGG CCGGCCGCTT CGAGCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTAAAAT CGATAAGGAT CCGTCGACCG ATGCCCTTGA GAGCCTTCAA CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG CATGACTATC GTCGCCGCAC TTATGACTGT CTTCTTTATC ATGCAACTCG TAGGACAGGT GCCGGCAGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGCGGCCGCA AATGCTAAAC CACTGCAGTG GTTACCAGTG CTTGATCAGT GAGGCACCGA TCTCAGCGAT CTGCCTATTT CGTTCGTCCA TAGTGGCCTG ACTCCCCGTC GTGTAGATCA CTACGATTCG TGAGGGCTTA CCATCAGGCC CCAGCGCAGC AATGATGCCG CGAGAGCCGC GTTCACCGGC CCCCGATTTG TCAGCAATGA ACCAGCCAGC AGGGAGGGCC GAGCGAAGAA GTGGTCCTGC TACTTTGTCC GCCTCCATCC AGTCTATGAG CTGCTGTCGT GATGCTAGAG TAAGAAGTTC GCCAGTGAGT AGTTTCCGAA GAGTTGTGGC CATTGCTACT GGCATCGTGG TATCACGCTC GTCGTTCGGT ATGGCTTCGT TCAACTCTGG TTCCCAGCGG TCAAGCCGGG TCACATGATC ACCCATATTA TGAAGAAATG CAGTCAGCTC CTTAGGGCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCG GTGTTGTCGC TCATGGTAAT GGCAGCACTA CACAATTCTC TTACCGTCAT GCCATCCGTA AGATGCTTTT CCGTGACCGG CGAGTACTCA ACCAAGTCGT TTTGTGAGTA GTGTATACGG CGACCAAGCT GCTCTTGCCC GGCGTCTATA CGGGACAACA CCGCGCCACA TAGCAGTACT TTGAAAGTGC TCATCATCGG GAATCGTTCT TCGGGGCGGA AAGACTCAAG GATCTTGCCG CTATTGAGAT CCAGTTCGAT ATAGCCCACT CTTGCACCCA GTTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCGGG GTGTGCAAAA ACAGGCAAGC AAAATGCCGC AAAGAAGGGA ATGAGTGCGA CACGAAAATG TTGGATGCTC ATACTCGTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTACTAGTA CGTCTCTCAA GGATAAGTAA GTAATATTAA GGTACGGGAG GTATTGGACA GGCCGCAATA AAATATCTTT ATTTTCATTA CATCTGTGTG TTGGTTTTTT GTGTGAATCG ATAGTACTAA CATACGCTCT CCATCAAAAC AAAACGAAAC AAAACAAACT AGCAAAATAG GCTGTCCCCA GTGCAAGTGC AGGTGCCAGA ACATTTCTCT
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