pHCMV-Env-TagRFP
$70.00
In stock
SKU
PL28537
·
100μg liquid plasmid(Instantly):$91
| name | pHCMV-Env-TagRFP | another name | apHCMV-Env-TagRFP |
| Insert Size (bp) | Plasmid host | Mammary cell,Lentiviral | |
| Plasmid uses | packaging aid | Fragment type | ORF |
| Fragment species | Virus | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Red | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pHCMV-EcoEnv | 5′ sequencing primer | PSG5-F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GAGCTCCACC GCGGTGGCGG CCGCTCTAGA GAGCTTGGCC CATTGCATAC GTTGTATCCA TATCATAATA TGTACATTTA TATTGGCTCA TGTCCAACAT TACCGCCATG TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTATG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACATCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAC AACTCCGCCC CATTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGATCGCC TGGAGACGCC ATCCACGCTG TTTTGACCTC CATAGAAGAC ACCGGGACCG ATCCAGCCTC CGGTCGACCG ATCCTGAGAA CTTCAGGGTG AGTTTGGGGA CCCTTGATTG TTCTTTCTTT TTCGCTATTG TAAAATTCAT GTTATATGGA GGGGGCAAAG TTTTCAGGGT GTTGTTTAGA ATGGGAAGAT GTCCCTTGTA TCACCATGGA CCCTCATGAT AATTTTGTTT CTTTCACTTT CTACTCTGTT GACAACCATT GTCTCCTCTT ATTTTCTTTT CATTTTCTGT AACTTTTTCG TTAAACTTTA GCTTGCATTT GTAACGAATT TTTAAATTCA CTTTTGTTTA TTTGTCAGAT TGTAAGTACT TTCTCTAATC ACTTTTTTTT CAAGGCAATC AGGGTATATT ATATTGTACT TCAGCACAGT TTTAGAGAAC AATTGTTATA ATTAAATGAT AAGGTAGAAT ATTTCTGCAT ATAAATTCTG GCTGGCGTGG AAATATTCTT ATTGGTAGAA ACAACTACAC CCTGGTCATC ATCCTGCCTT TCTCTTTATG GTTACAATGA TATACACTGT TTGAGATGAG GATAAAATAC TCTGAGTCCA AACCGGGCCC CTCTGCTAAC CATGTTCATG CCTTCTTCTC TTTCCTACAG CTCCTGGGCA ACGTGCTGGT TGTTGTGCTG TCTCATCATT TTGGCAAAGA ATTCCGCGGG CGGCCGCTCT AGAGGATCCG CTAGACTGAC ATGGCGTGTT CAACGCTCCC AAAATCCCCT AAAGATAAGA TTGACCCGCG GGACCTCCTA ATCCCCTTAA TTCTCTTCCT GTCTCTCAAA GGGGCCAGAT CCGCAGCACC CGGCTCCAGC CCTCACCAGG TCTACAACAT TACCTGGGAA GTGACCAATG GGGATCGGGA GACAGTATGG GCAATATCAG GCAACCACCC TCTGTGGACT TGGTGGCCAG TCCTCACCCC AGATTTGTGT ATGTTAGCTC TCAGTGGGCC GCCCCACTGG GGGCTAGAGT ATCAGGCCCC CTATTCCTCG CCCCCGGGGC CCCCTTGTTG CTCAGGGAGC AGCGGGAGCA GTGCAGGCTG TTCCAGAGAC TGCGACGAGC CCTTGACCTC CCTCACCCCT CGGTGCAACA CTGCCTGGAA CAGACTTAAG CTAGATCAGG TAACTCATAA ATCAAGTGAG GGATTTTATG TCTGCCCCGG GTCACATCGC CCCCGGGAAG CCAAGTCCTG TGGAGGTCCA GACTCCTTCT ACTGTGCCTC TTGGGGCTGC GAGACAACCG GTAGAGTATA CTGGAAGCCC TCCTCCTCTT GGGACTACAT CACAGTGGAC AACAATCTCA CCACTAGCCA GGCTGTCCAG GTATGCAAAG ACAATAAGTG GTGCAATCCC TTGGCTATCC AGTTTACAAA CGCCGGGAAA CAGGTCACCT CATGGACAAC TGGACACTAT TGGGGTCTAC GTCTTTATGT CTCTGGGCGG GACCCGGGGC TTACTTTCGG GATCCGACTC AGATATCAAA ATCTAGGACC TCGGGTCCCG ATAGGACCGA ACCCCGTCCT GGCAGACCAA CTTTCGCTCC CGCGACCTAA TCCCCTACCC AAACCTGCCA AGTCTCCCCC CGCCTCTAAT TCGACTCCCA CATTGATTTC CGCTAGCATG GTGTCTAAGG GCGAAGAGCT GATTAAGGAG AACATGCACA TGAAGCTGTA CATGGAGGGC ACCGTGAACA ACCACCACTT CAAGTGCACA TCCGAGGGCG AAGGCAAGCC CTACGAGGGC ACCCAGACCA TGAGAATCAA GGTGGTCGAG GGCGGCCCTC TCCCCTTCGC CTTCGACATC CTGGCTACCA GCTTCATGTA CGGCAGCAGA ACCTTCATCA ACCACACCCA GGGCATCCCC GACTTCTTTA AGCAGTCCTT CCCTGAGGGC TTCACATGGG AGAGAGTCAC CACATACGAA GACGGGGGCG TGCTGACCGC TACCCAGGAC ACCAGCCTCC AGGACGGCTG CCTCATCTAC AACGTCAAGA TCAGAGGGGT GAACTTCCCA TCCAACGGCC CTGTGATGCA GAAGAAAACA CTCGGCTGGG AGGCCAACAC CGAGATGCTG TACCCCGCTG ACGGCGGCCT GGAAGGCAGA AGCGACATGG CCCTGAAGCT CGTGGGCGGG GGCCACCTGA TCTGCAACTT CAAGACCACA TACAGATCCA AGAAACCCGC TAAGAACCTC AAGATGCCCG GCGTCTACTA TGTGGACCAC AGACTGGAAA GAATCAAGGA GGCCGACAAA GAGACCTACG TCGAGCAGCA CGAGGTGGCT GTGGCCAGAT ACTGCGACCT CCCTAGCAAA CTGGGGCACA AACTTAATGC TAGCGCTGTT GCCGGCGCCC CGTCCCCCAC TCCCACTCAG CCCCCGCCAG CAGGAACGGG AGACAGGTTA CTAAATCTAG TACAGGGAGC TTACCAGGCA CTCAACCTTA CCAACCCTGA TAAAACTCAA GAGTGCTGGT TATGCCTAGT GTCTGGACCC CCCTATTACG AAGGGGTTGC CGTCCTAGGT ACTTATTCCA ACCATACCTC TGCCCCAGCT AACTGCTCCG TGGCCTCCCA ACACAAGTTG ACCCTGTCCG AAGTGACTGG ACGGGGACTC TGCATAGGAA CAGTCCCAAA AACTCACCAG GCCCTGTGCA ACACTACCCT TAAGATAGAC AAAGGGTCTT ACTATCTAGT TGCCCCCACA GGAACTACGT GGGCATGTAA CACTGGACTC ACTCCATGCC TATCTGCCAC CGTGCTTAAT CGCACCACTG ACTATTGCGT TCTCGTAGAG TTATGGCCCA GGGTCACCTA CCATCCTCCC AGTTACGTCT ATAGCCAGTT TGAAAAATCC TATAGACATA AAAGAGAACC AGTGTCCTTA ACCTTGGCCC TATTATTAGG TGGGCTAACT ATGGGTGGCA TCGCCGCGGG AGTAGGGACA GGAACTACCG CCCTGGTCGC CACCCAGCAG TTCCAGCAGC TCCATGCTGC CGTACAAGAT GATCTCAAAG AAGTCGAAAA GTCAATTACT AACCTAGAAA AGTCTCTTAC TTCGTTGTCT GAGGTTGTGC TGCAGAATCG ACGAGGCCTA GACCTGTTGT TCCTAAAAGA AGGAGGACTG TGTGCTGCCC TAAAAGAAGA ATGTTGTTTC TATGCTGACC ATACAGGCCT AGTAAGAGAT AGTATGGCCA AATTAAGAGA GAGACTCACT CAGAGACAAA AACTATTTGA GTCGAGCCAA GGATGGTTCG AAGGATTGTT TAACAGATCC CCCTGGTTTA CCACGTTAAT ATCCACCATC ATGGGGCCTC TCATTATACT CCTACTAATT CTGCTTTTTG GACCCTGCAT TCTTAATCGA TTAGTCCAAT TTGTTAAAGA CAGGATATCA GTGGTCCAGG CTCTAGTTTT GACTCAACAA TATCACCAGC TGAAGCCACT AGAATACGAG CCATGACTCG AGGGCCCAGA TCTTTTTCCC TCTGCCAAAA ATTATGGGGA CATCATGAAG CCCCTTGAGC ATCTGACTTC TGGCTAATAA AGGAAATTTA TTTTCATTGC AATAGTGTGT TGGAATTTTT TGTGTCTCTC ACTCGGAAGG ACATATGGGA GGGCAAATCA TTTAAAACAT CAGAATGAGT ATTTGGTTTA GAGTTTGGCA ACATATGCCC ATATGCTGGC TGCCATGAAC AAAGGTTGGC TATAAAGAGG TCATCAGTAT ATGAAACAGC CCCCTGCTGT CCATTCCTTA TTCCATAGAA AAGCCTTGAC TTGAGGTTAG ATTTTTTTTA TATTTTGTTT TGTGTTATTT TTTTCTTTAA CATCCCTAAA ATTTTCCTTA CATGTTTTAC TAGCCAGATT TTTCCTCCTC TCCTGACTAC TCCCAGTCAT AGCTGTCCCT CTTCTCTTAT GGAGATCCCT CGACGGATCG GCCGCAATTC GTAATCATGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAGCGC GCGTAATACG ACTCACTATA GGGCGAATTG
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
