pET28a-TEV-AsCpf1-6×His
$55.00
In stock
SKU
PL28522
·
100μg liquid plasmid(Instantly):$98
| name | pET28a-TEV-AsCpf1-6×His | another name | apET28a-TEV-AsCpf1-6×His |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Fluorescent labeling | ||
| Promoter | T7 | Replicon | pBR322 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pET-28a | 5′ sequencing primer | T7 |
| 3′ sequencing primer | Copy number | Low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCAGCA GCCATCATCA TCATCATCAC AGCAGCGGCC TGGTGCCGCG CGGCAGCCAT ATGGAAAACC TGTATTTTCA GGGCATGACA CAGTTCGAGG GCTTTACCAA CCTGTATCAG GTGAGCAAGA CACTGCGGTT TGAGCTGATC CCACAGGGCA AGACCCTGAA GCACATCCAG GAGCAGGGCT TCATCGAGGA GGACAAGGCC CGCAATGATC ACTACAAGGA GCTGAAGCCC ATCATCGATC GGATCTACAA GACCTATGCC GACCAGTGCC TGCAGCTGGT GCAGCTGGAT TGGGAGAACC TGAGCGCCGC CATCGACTCC TATAGAAAGG AGAAAACCGA GGAGACAAGG AACGCCCTGA TCGAGGAGCA GGCCACATAT CGCAATGCCA TCCACGACTA CTTCATCGGC CGGACAGACA ACCTGACCGA TGCCATCAAT AAGAGACACG CCGAGATCTA CAAGGGCCTG TTCAAGGCCG AGCTGTTTAA TGGCAAGGTG CTGAAGCAGC TGGGCACCGT GACCACAACC GAGCACGAGA ACGCCCTGCT GCGGAGCTTC GACAAGTTTA CAACCTACTT CTCCGGCTTT TATGAGAACA GGAAGAACGT GTTCAGCGCC GAGGATATCA GCACAGCCAT CCCACACCGC ATCGTGCAGG ACAACTTCCC CAAGTTTAAG GAGAATTGTC ACATCTTCAC ACGCCTGATC ACCGCCGTGC CCAGCCTGCG GGAGCACTTT GAGAACGTGA AGAAGGCCAT CGGCATCTTC GTGAGCACCT CCATCGAGGA GGTGTTTTCC TTCCCTTTTT ATAACCAGCT GCTGACACAG ACCCAGATCG ACCTGTATAA CCAGCTGCTG GGAGGAATCT CTCGGGAGGC AGGCACCGAG AAGATCAAGG GCCTGAACGA GGTGCTGAAT CTGGCCATCC AGAAGAATGA TGAGACAGCC CACATCATCG CCTCCCTGCC ACACAGATTC ATCCCCCTGT TTAAGCAGAT CCTGTCCGAT AGGAACACCC TGTCTTTCAT CCTGGAGGAG TTTAAGAGCG ACGAGGAAGT GATCCAGTCC TTCTGCAAGT ACAAGACACT GCTGAGAAAC GAGAACGTGC TGGAGACAGC CGAGGCCCTG TTTAACGAGC TGAACAGCAT CGACCTGACA CACATCTTCA TCAGCCACAA GAAGCTGGAG ACAATCAGCA GCGCCCTGTG CGACCACTGG GATACACTGA GGAATGCCCT GTATGAGCGG AGAATCTCCG AGCTGACAGG CAAGATCACC AAGTCTGCCA AGGAGAAGGT GCAGCGCAGC CTGAAGCACG AGGATATCAA CCTGCAGGAG ATCATCTCTG CCGCAGGCAA GGAGCTGAGC GAGGCCTTCA AGCAGAAAAC CAGCGAGATC CTGTCCCACG CACACGCCGC CCTGGATCAG CCACTGCCTA CAACCCTGAA GAAGCAGGAG GAGAAGGAGA TCCTGAAGTC TCAGCTGGAC AGCCTGCTGG GCCTGTACCA CCTGCTGGAC TGGTTTGCCG TGGATGAGTC CAACGAGGTG GACCCCGAGT TCTCTGCCCG GCTGACCGGC ATCAAGCTGG AGATGGAGCC TTCTCTGAGC TTCTACAACA AGGCCAGAAA TTATGCCACC AAGAAGCCCT ACTCCGTGGA GAAGTTCAAG CTGAACTTTC AGATGCCTAC ACTGGCCTCT GGCTGGGACG TGAATAAGGA GAAGAACAAT GGCGCCATCC TGTTTGTGAA GAACGGCCTG TACTATCTGG GCATCATGCC AAAGCAGAAG GGCAGGTATA AGGCCCTGAG CTTCGAGCCC ACAGAGAAAA CCAGCGAGGG CTTTGATAAG ATGTACTATG ACTACTTCCC TGATGCCGCC AAGATGATCC CAAAGTGCAG CACCCAGCTG AAGGCCGTGA CAGCCCACTT TCAGACCCAC ACAACCCCCA TCCTGCTGTC CAACAATTTC ATCGAGCCTC TGGAGATCAC AAAGGAGATC TACGACCTGA ACAATCCTGA GAAGGAGCCA AAGAAGTTTC AGACAGCCTA CGCCAAGAAA ACCGGCGACC AGAAGGGCTA CAGAGAGGCC CTGTGCAAGT GGATCGACTT CACAAGGGAT TTTCTGTCCA AGTATACCAA GACAACCTCT ATCGATCTGT CTAGCCTGCG GCCATCCTCT CAGTATAAGG ACCTGGGCGA GTACTATGCC GAGCTGAATC CCCTGCTGTA CCACATCAGC TTCCAGAGAA TCGCCGAGAA GGAGATCATG GATGCCGTGG AGACAGGCAA GCTGTACCTG TTCCAGATCT ATAACAAGGA CTTTGCCAAG GGCCACCACG GCAAGCCTAA TCTGCACACA CTGTATTGGA CCGGCCTGTT TTCTCCAGAG AACCTGGCCA AGACAAGCAT CAAGCTGAAT GGCCAGGCCG AGCTGTTCTA CCGCCCTAAG TCCAGGATGA AGAGGATGGC ACACCGGCTG GGAGAGAAGA TGCTGAACAA GAAGCTGAAG GATCAGAAAA CCCCAATCCC CGACACCCTG TACCAGGAGC TGTACGACTA TGTGAATCAC AGACTGTCCC ACGACCTGTC TGATGAGGCC AGGGCCCTGC TGCCCAACGT GATCACCAAG GAGGTGTCTC ACGAGATCAT CAAGGATAGG CGCTTTACCA GCGACAAGTT CTTTTTCCAC GTGCCTATCA CACTGAACTA TCAGGCCGCC AATTCCCCAT CTAAGTTCAA CCAGAGGGTG AATGCCTACC TGAAGGAGCA CCCCGAGACA CCTATCATCG GCATCGATCG GGGCGAGAGA AACCTGATCT ATATCACAGT GATCGACTCC ACCGGCAAGA TCCTGGAGCA GCGGAGCCTG AACACCATCC AGCAGTTTGA TTACCAGAAG AAGCTGGACA ACAGGGAGAA GGAGAGGGTG GCAGCAAGGC AGGCCTGGTC TGTGGTGGGC ACAATCAAGG ATCTGAAGCA GGGCTATCTG AGCCAGGTCA TCCACGAGAT CGTGGACCTG ATGATCCACT ACCAGGCCGT GGTGGTGCTG GAGAACCTGA ATTTCGGCTT TAAGAGCAAG AGGACCGGCA TCGCCGAGAA GGCCGTGTAC CAGCAGTTCG AGAAGATGCT GATCGATAAG CTGAATTGCC TGGTGCTGAA GGACTATCCA GCAGAGAAAG TGGGAGGCGT GCTGAACCCA TACCAGCTGA CAGACCAGTT CACCTCCTTT GCCAAGATGG GCACCCAGTC TGGCTTCCTG TTTTACGTGC CTGCCCCATA TACATCTAAG ATCGATCCCC TGACCGGCTT CGTGGACCCC TTCGTGTGGA AAACCATCAA GAATCACGAG AGCCGCAAGC ACTTCCTGGA GGGCTTCGAC TTTCTGCACT ACGACGTGAA AACCGGCGAC TTCATCCTGC ACTTTAAGAT GAACAGAAAT CTGTCCTTCC AGAGGGGCCT GCCCGGCTTT ATGCCTGCAT GGGATATCGT GTTCGAGAAG AACGAGACAC AGTTTGACGC CAAGGGCACC CCTTTCATCG CCGGCAAGAG AATCGTGCCA GTGATCGAGA ATCACAGATT CACCGGCAGA TACCGGGACC TGTATCCTGC CAACGAGCTG ATCGCCCTGC TGGAGGAGAA GGGCATCGTG TTCAGGGATG GCTCCAACAT CCTGCCAAAG CTGCTGGAGA ATGACGATTC TCACGCCATC GACACCATGG TGGCCCTGAT CCGCAGCGTG CTGCAGATGC GGAACTCCAA TGCCGCCACA GGCGAGGACT ATATCAACAG CCCCGTGCGC GATCTGAATG GCGTGTGCTT CGACTCCCGG TTTCAGAACC CAGAGTGGCC CATGGACGCC GATGCCAATG GCGCCTACCA CATCGCCCTG AAGGGCCAGC TGCTGCTGAA TCACCTGAAG GAGAGCAAGG ATCTGAAGCT GCAGAACGGC ATCTCCAATC AGGACTGGCT GGCCTACATC CAGGAGCTGC GCAACAAGCG ACCTGCCGCC ACAAAGAAGG CTGGACAGGC TAAGAAGAAG AAACTCGAGC ACCACCACCA CCACCACTGA GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGAT
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
