pTD103luxI
$55.00
In stock
SKU
PL28515
·
100μg liquid plasmid(Instantly):$98
| name | pTD103luxI | another name | apTD103luxI |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Signal report | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Luxlp | Replicon | P15A |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pTD103luxI_sfGFP | 5′ sequencing primer | |
| 3′ sequencing primer | Copy number | Low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTCGAGTTAA TTTTTAAAGT ATGGGCAATC AATTGCTCCT GTTAAAATTG CTTTAGAAAT ACTTTGGCAG CGGTTTGTTG TATTGAGTTT CATTTGCGCA TTGGTTAAAT GGAAAGTGAC AGTACGCTCA CTGCAGCCTA ATATTTTTGA AATATCCCAA GAGCTTTTTC CTTCGCATGC CCACGCTAAA CATTCTTTTT CTCTTTTGGT TAAATCGTTG TTTGATTTAT TATTTGCTAT ATTTATTTTT CGATAATTAT CAACTAGAGA AGGAACAATT AATGGTATGT TCATACACGC ATGTAAAAAT AAACTATCTA TATAGTTGTC TTTTTCTGAA TGTGCAAAAC TAAGCATTCC GAAGCCATTG TTAGCCGTAT GAATAGGGAA ACTAAACCCA GTGATAAGAC CTGATGTTTT CGCTTCTTTA ATTACATTTG GAGATTTTTT ATTTACAGCA TTGTTTTCAA ATATATTCCA ATTAATTGGT GAATGATTGG AGTTAGAATA ATCTACTATA GGATCATATT TTATTAAATT AGCGTCATCA TAATATTGCC TCCATTTTTT AGGGTAATTA TCTAGAATTG AAATATCAGA TTTAACCATA GAATGAGGAT AAATGATCGC GAGTAAATAA TATTCACAAT GTACCATTTT AGTCATATCA GATAAGCATT GATTAATATC ATTATTGCTT CTACAAGCTT TAATTTTATT AATTATTCTG TATGTGTCGT CGGCATTTAT GTTTTTCATA CCCATCTCTT TATCCTTACC TATTGTTTGT CGCAAGTTTT GCGTGTTATA TATCATTAAA ACGGTAATGG ATTGACATTT GATTCTAATA AATTGGATTT TTGTCACACT ATTGTATCGC TGGGAATACA ATTACTTAAC ATAAGCACCT GTAGGATCGT ACAGGTTTAC GCAAGAAAAT GGTTTGTTAT AGTCGAATGA ATTCATTAAA GAGGAGAAAG GTACCGCAGC GAACGACGAA AATTACGCCC TTGCAGCGTA AAAGCTTAAT TAGCTGATCT AGACGCGTGC TAGAGGCATC AAATAAAACG AAAGGCTCAG TCGAAAGACT GGGCCTTTCG TTTTATCTGT TGTTTGTCGG TGAACGCTCT CCTGAGTAGG ACAAATCCGC CGCCCTAGAC CTAGCCGTCT TCACCTCGAG TTAATTTTTA AAGTATGGGC AATCAATTGC TCCTGTTAAA ATTGCTTTAG AAATACTTTG GCAGCGGTTT GTTGTATTGA GTTTCATTTG CGCATTGGTT AAATGGAAAG TGACAGTACG CTCACTGCAG CCTAATATTT TTGAAATATC CCAAGAGCTT TTTCCTTCGC ATGCCCACGC TAAACATTCT TTTTCTCTTT TGGTTAAATC GTTGTTTGAT TTATTATTTG CTATATTTAT TTTTCGATAA TTATCAACTA GAGAAGGAAC AATTAATGGT ATGTTCATAC ACGCATGTAA AAATAAACTA TCTATATAGT TGTCTTTTTC TGAATGTGCA AAACTAAGCA TTCCGAAGCC ATTGTTAGCC GTATGAATAG GGAAACTAAA CCCAGTGATA AGACCTGATG TTTTCGCTTC TTTAATTACA TTTGGAGATT TTTTATTTAC AGCATTGTTT TCAAATATAT TCCAATTAAT TGGTGAATGA TTGGAGTTAG AATAATCTAC TATAGGATCA TATTTTATTA AATTAGCGTC ATCATAATAT TGCCTCCATT TTTTAGGGTA ATTATCTAGA ATTGAAATAT CAGATTTAAC CATAGAATGA GGATAAATGA TCGCGAGTAA ATAATATTCA CAATGTACCA TTTTAGTCAT ATCAGATAAG CATTGATTAA TATCATTATT GCTTCTACAA GCTTTAATTT TATTAATTAT TCTGTATGTG TCGTCGGCAT TTATGTTTTT CATACCCATC TCTTTATCCT TACCTATTGT TTGTCGCAAG TTTTGCGTGT TATATATCAT TAAAACGGTA ATGGATTGAC ATTTGATTCT AATAAATTGG ATTTTTGTCA CACTATTGTA TCGCTGGGAA TACAATTACT TAACATAAGC ACCTGTAGGA TCGTACAGGT TTACGCAAGA AAATGGTTTG TTATAGTCGA ATGAATTCAT TAAAGAGGAG AAAGGTACCA TGACTATAAT GATAAAAAAA TCGGATTTTT TGGCAATTCC ATCGGAGGAG TATAAAGGTA TTCTAAGTCT TCGTTATCAA GTGTTTAAGC AAAGACTTGA GTGGGACTTA GTTGTAGAAA ATAACCTTGA ATCAGATGAG TATGATAACT CAAATGCAGA ATATATTTAT GCTTGTGATG ATACTGAAAA TGTAAGTGGA TGCTGGCGTT TATTACCTAC AACAGGTGAT TATATGCTGA AAAGTGTTTT TCCTGAATTG CTTGGTCAAC AGAGTGCTCC CAAAGATCCT AATATAGTCG AATTAAGTCG TTTTGCTGTA GGTAAAAATA GCTCAAAGAT AAATAACTCT GCTAGTGAAA TTACAATGAA ACTATTTGAA GCTATATATA AACACGCTGT TAGTCAAGGT ATTACAGAAT ATGTAACAGT AACATCAACA GCAATAGAGC GATTTTTAAA GCGTATTAAA GTTCCTTGTC ATCGTATTGG AGACAAAGAA ATTCATGTAT TAGGTGATAC TAAATCGGTT GTATTGTCTA TGCCTATTAA TGAACAGTTT AAAAAAGCAG TCTTAAATGC AGCGAACGAC GAAAATTACG CCCTTGCAGC GTAAACGCGT GCTAGAGGCA TCAAATAAAA CGAAAGGCTC AGTCGAAAGA CTGGGCCTTT CGTTTTATCT GTTGTTTGTC GGTGAACGCT CTCCTGAGTA GGACAAATCC GCCGCCCTAG ACCTAGGGCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGACTA GTGCTTGGAT TCTCACCAAT AAAAAACGCC CGGCGGCAAC CGAGCGTTCT GAACAAATCC AGATGGAGTT CTGAGGTCAT TACTGGATCT ATCAACAGGA GTCCAAGCGA GCTCTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG GTCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCG CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCCTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCAG ATCTTGATCC CCTGCGCCAT CAGATCCTTG GCGGCAAGAA AGCCATCCAG TTTACTTTGC AGGGCTTCCC AACCTTACCA GAGGGCGCCC CAGCTGGCAA TTCCGACGTC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTTCAC
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