pMSCV-ERN1(human)-HA-Hyg
$84.00
In stock
SKU
PL28514
NM_001433.5;hIRE1p; IRE1; IRE1a; IRE1P
100μg liquid plasmid(Instantly):$105
| name | pMSCV-ERN1(human)-HA-Hyg | another name | apMSCV-ERN1(human)-HA-Hyg |
| Insert Size (bp) | 2931bp | Plasmid host | Mammary cell,retrovirus |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | Replicon | pUC | |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | pMSCV-Hyg | 5′ sequencing primer | PLXSN-F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT TGCAAGGCAT GGAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG TTAGGAACAG AGAGACAGCA GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC TAGAGAACCA TCAGATGTTT CCAGGGTGCC CCAAGGACCT GAAATGACCC TGTGCCTTAT TTGAACTAAC CAATCAGTTC GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC AATAAAAGAG CCCACAACCC CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC GGGTACCCGT ATTCCCAATA AAGCCTCTTG CTGTTTGCAT CCGAATCGTG GACTCGCTGA TCCTTGGGAG GGTCTCCTCA GATTGATTGA CTGCCCACCT CGGGGGTCTT TCATTTGGAG GTTCCACCGA GATTTGGAGA CCCCTGCCCA GGGACCACCG ACCCCCCCGC CGGGAGGTAA GCTGGCCAGC GGTCGTTTCG TGTCTGTCTC TGTCTTTGTG CGTGTTTGTG CCGGCATCTA ATGTTTGCGC CTGCGTCTGT ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG TGGAACTGAC GAGTTCTGAA CACCCGGCCG CAACCCTGGG AGACGTCCCA GGGACTTTGG GGGCCGTTTT TGTGGCCCGA CCTGAGGAAG GGAGTCGATG TGGAATCCGA CCCCGTCAGG ATATGTGGTT CTGGTAGGAG ACGAGAACCT AAAACAGTTC CCGCCTCCGT CTGAATTTTT GCTTTCGGTT TGGAACCGAA GCCGCGCGTC TTGTCTGCTG CAGCGCTGCA GCATCGTTCT GTGTTGTCTC TGTCTGACTG TGTTTCTGTA TTTGTCTGAA AATTAGGGCC AGACTGTTAC CACTCCCTTA AGTTTGACCT TAGGTCACTG GAAAGATGTC GAGCGGATCG CTCACAACCA GTCGGTAGAT GTCAAGAAGA GACGTTGGGT TACCTTCTGC TCTGCAGAAT GGCCAACCTT TAACGTCGGA TGGCCGCGAG ACGGCACCTT TAACCGAGAC CTCATCACCC AGGTTAAGAT CAAGGTCTTT TCACCTGGCC CGCATGGACA CCCAGACCAG GTCCCCTACA TCGTGACCTG GGAAGCCTTG GCTTTTGACC CCCCTCCCTG GGTCAAGCCC TTTGTACACC CTAAGCCTCC GCCTCCTCTT CCTCCATCCG CCCCGTCTCT CCCCCTTGAA CCTCCTCGTT CGACCCCGCC TCGATCCTCC CTTTATCCAG CCCTCACTCC TTCTCTAGGC GCCGGAATTA GATCTATGCC GGCCCGGCGG CTGCTGCTGC TGCTGACGCT GCTGCTGCCC GGCCTCGGGA TTTTTGGAAG TACCAGCACA GTGACGCTTC CTGAAACCTT GTTGTTTGTG TCAACGCTGG ATGGAAGTTT GCATGCTGTC AGCAAGAGGA CAGGCTCAAT CAAATGGACT TTAAAAGAAG ATCCAGTCCT GCAGGTCCCA ACACATGTGG AAGAGCCTGC CTTTCTCCCA GATCCTAATG ATGGCAGCCT GTATACGCTT GGAAGCAAGA ATAATGAAGG CCTGACGAAA CTTCCTTTTA CCATCCCAGA ATTGGTGCAG GCATCCCCAT GCCGAAGTTC AGATGGAATC CTCTACATGG GTAAAAAGCA GGACATCTGG TATGTTATTG ACCTCCTGAC CGGAGAGAAG CAGCAGACTT TGTCATCGGC CTTTGCAGAT AGTCTCTGCC CATCAACCTC TCTTCTGTAT CTTGGGCGAA CAGAATACAC CATCACCATG TACGACACCA AAACCCGAGA GCTCCGGTGG AATGCCACCT ACTTTGACTA TGCGGCCTCA CTGCCTGAGG ACGACGTGGA CTACAAGATG TCCCACTTTG TGTCCAATGG TGATGGGCTG GTGGTGACTG TGGACAGTGA ATCTGGGGAC GTCCTGTGGA TCCAAAACTA CGCCTCCCCT GTGGTGGCCT TTTATGTCTG GCAGCGGGAG GGTCTGAGGA AGGTGATGCA CATCAATGTC GCTGTGGAGA CCCTGCGCTA TCTGACCTTC ATGTCTGGGG AGGTGGGGCG CATCACAAAG TGGAAGTACC CGTTCCCCAA GGAGACAGAG GCCAAGAGCA AGCTGACGCC CACTCTGTAT GTTGGGAAAT ACTCTACCAG CCTCTATGCC TCTCCCTCAA TGGTACACGA GGGGGTTGCT GTCGTGCCCC GCGGCAGCAC ACTTCCTTTG CTGGAAGGGC CCCAGACTGA TGGCGTCACC ATTGGGGACA AGGGGGAGTG TGTGATCACG CCCAGCACGG ACGTCAAGTT TGATCCCGGA CTCAAAAGCA AGAACAAGCT CAACTACTTG AGGAATTACT GGCTTCTGAT AGGACACCAT GAAACCCCAC TGTCTGCGTC TACCAAGATG CTGGAGAGAT TTCCCAACAA TCTACCCAAA CATCGGGAAA ATGTGATTCC TGCTGATTCA GAGAAAAAGA GCTTTGAGGA AGTTATCAAC CTGGTTGACC AGACTTCAGA AAACGCACCT ACCACCGTGT CTCGGGATGT GGAGGAGAAG CCCGCCCATG CCCCTGCCCG GCCCGAGGCC CCCGTGGACT CCATGCTTAA GGACATGGCT ACCATCATCC TGAGCACCTT CCTGCTGATT GGCTGGGTGG CCTTCATCAT CACCTATCCC CTGAGCATGC ATCAGCAGCA GCAGCTCCAG CACCAGCAGT TCCAGAAGGA ACTGGAGAAG ATCCAGCTCC TGCAGCAGCA GCAGCAGCAG CTGCCCTTCC ACCCACCTGG AGACACGGCT CAGGACGGCG AGCTCCTGGA CACGTCTGGC CCGTACTCAG AGAGCTCGGG CACCAGCAGC CCCAGCACGT CCCCCAGGGC CTCCAACCAC TCGCTCTGCT CCGGCAGCTC TGCCTCCAAG GCTGGCAGCA GCCCCTCCCT GGAACAAGAC GATGGAGATG AGGAAACCAG CGTGGTGATA GTTGGGAAAA TTTCCTTCTG TCCCAAGGAT GTCCTGGGCC ATGGAGCTGA GGGCACAATT GTGTACCGGG GCATGTTTGA CAACCGCGAC GTGGCCGTGA AGAGGATCCT CCCCGAGTGT TTTAGCTTCG CAGACCGTGA GGTCCAGCTG TTGCGAGAAT CGGATGAGCA CCCGAACGTG ATCCGCTACT TCTGCACGGA GAAGGACCGG CAATTCCAGT ACATTGCCAT CGAGCTGTGT GCAGCCACCC TGCAAGAGTA TGTGGAGCAG AAGGACTTTG CGCATCTCGG CCTGGAGCCC ATCACCTTGC TGCAGCAGAC CACCTCGGGC CTGGCCCACC TCCACTCCCT CAACATCGTT CACAGAGACC TAAAGCCACA CAACATCCTC ATATCCATGC CCAATGCACA CGGCAAGATC AAGGCCATGA TCTCCGACTT TGGCCTCTGC AAGAAGCTGG CAGTGGGCAG ACACAGTTTC AGCCGCCGAT CTGGGGTGCC TGGCACAGAA GGCTGGATCG CTCCAGAGAT GCTGAGCGAA GACTGTAAGG AGAACCCTAC CTACACGGTG GACATCTTTT CTGCAGGCTG CGTCTTTTAC TACGTAATCT CTGAGGGCAG CCACCCTTTT GGCAAGTCCC TGCAGCGGCA GGCCAACATC CTCCTGGGTG CCTGCAGCCT TGACTGCTTG CACCCAGAGA AGCACGAAGA CGTCATTGCA CGTGAATTGA TAGAGAAGAT GATTGCGATG GATCCTCAGA AACGCCCCTC AGCGAAGCAT GTGCTCAAAC ACCCGTTCTT CTGGAGCCTA GAGAAGCAGC TCCAGTTCTT CCAGGACGTG AGCGACAGAA TAGAAAAGGA ATCCCTGGAT GGCCCGATCG TGAAGCAGTT AGAGAGAGGC GGGAGAGCCG TGGTGAAGAT GGACTGGCGG GAGAACATCA CTGTCCCCCT CCAGACAGAC CTGCGTAAAT TCAGGACCTA TAAAGGTGGT TCTGTCAGAG ATCTCCTCCG AGCCATGAGA AATAAGAAGC ACCACTACCG GGAGCTGCCT GCAGAGGTGC GGGAGACGCT GGGGTCCCTC CCCGACGACT TCGTGTGCTA CTTCACATCT CGCTTCCCCC ACCTCCTCGC ACACACCTAC CGGGCCATGG AGCTGTGCAG CCACGAGAGA CTCTTCCAGC CCTACTACTT CCACGAGCCC CCAGAGCCCC AGCCCCCAGT GACTCCAGAC GCCCTCGAAT TCGGTACCTA CCCATACGAT GTTCCAGATT ACGCTTAACT CGAGGTTAAC GAATTCTACC GGGTAGGGGA GGCGCTTTTC CCAAGGCAGT CTGGAGCATG CGCTTTAGCA GCCCCGCTGG GCACTTGGCG CTACACAAGT GGCCTCTGGC CTCGCACACA TTCCACATCC ACCGGTAGGC GCCAACCGGC TCCGTTCTTT GGTGGCCCCT TCGCGCCACC TTCTACTCCT CCCCTAGTCA GGAAGTTCCC CCCCGCCCCG CAGCTCGCGT CGTGCAGGAC GTGACAAATG GAAGTAGCAC GTCTCACTAG TCTCGTGCAG ATGGACAGCA CCGCTGAGCA ATGGAAGCGG GTAGGCCTTT GGGGCAGCGG CCAATAGCAG CTTTGCTCCT TCGCTTTCTG GGCTCAGAGG CTGGGAAGGG GTGGGTCCGG GGGCGGGCTC AGGGGCGGGC TCAGGGGCGG GGCGGGCGCC CGAAGGTCCT CCGGAGGCCC GGCATTCTGC ACGCTTCAAA AGCGCACGTC TGCCGCGCTG TTCTCCTCTT CCTCATCTCC GGGCCTTTCG ACCTGCATCC CGCCACCATG AAAAAGCCTG AACTCACCGC GACGTCTGTC GAGAAGTTTC TGATCGAAAA GTTCGACAGC GTCTCCGACC TGATGCAGCT CTCGGAGGGC GAAGAATCTC GTGCTTTCAG CTTCGATGTA GGAGGGCGTG GATATGTCCT GCGGGTAAAT AGCTGCGCCG ATGGTTTCTA CAAAGATCGT TATGTTTATC GGCACTTTGC ATCGGCCGCG CTCCCGATTC CGGAAGTGCT TGACATTGGG GAATTCAGCG AGAGCCTGAC CTATTGCATC TCCCGCCGTG CACAGGGTGT CACGTTGCAA GACCTGCCTG AAACCGAACT GCCCGCTGTT CTGCAGCCGG TCGCGGAGGC CATGGATGCG ATCGCTGCGG CCGATCTTAG CCAGACGAGC GGGTTCGGCC CATTCGGACC GCAAGGAATC GGTCAATACA CTACATGGCG TGATTTCATA TGCGCGATTG CTGATCCCCA TGTGTATCAC TGGCAAACTG TGATGGACGA CACCGTCAGT GCGTCCGTCG CGCAGGCTCT CGATGAGCTG ATGCTTTGGG CCGAGGACTG CCCCGAAGTC CGGCACCTCG TGCACGCGGA TTTCGGCTCC AACAATGTCC TGACGGACAA TGGCCGCATA ACAGCGGTCA TTGACTGGAG CGAGGCGATG TTCGGGGATT CCCAATACGA GGTCGCCAAC ATCTTCTTCT GGAGGCCGTG GTTGGCTTGT ATGGAGCAGC AGACGCGCTA CTTCGAGCGG AGGCATCCGG AGCTTGCAGG ATCGCCGCGG CTCCGGGCGT ATATGCTCCG CATTGGTCTT GACCAACTCT ATCAGAGCTT GGTTGACGGC AATTTCGATG ATGCAGCTTG GGCGCAGGGT CGATGCGACG CAATCGTCCG ATCCGGAGCC GGGACTGTCG GGCGTACACA AATCGCCCGC AGAAGCGCGG CCGTCTGGAC CGATGGCTGT GTAGAAGTAC TCGCCGATAG TGGAAACCGA CGCCCCAGCA CTCGTCCGAG GGCAAAGGAA TAGAGTAGAT GCCGACCGAA CAAGAGCTGA TTTCGAGAAC GCCTCAGCCA GCAACTCGCG CGAGCCTAGC AAGGCAAATG CGAGAGAACG GCCTTACGCT TGGTGGCACA GTTCTCGTCC ACAGTTCGCT AAGCTCGCTC GGCTGGGTCG CGGGAGGGCC GGTCGCAGTG ATTCAGGCCC TTCTGGATTG TGTTGGTCCC CAGGGCACGA TTGTCATGCC CACGCACTCG GGTGATCTGA CTGATCCCGC AGATTGGAGA TCGCCGCCCG TGCCTGCCGA TTGGGTGCAG ATCCGTCGAC CTGCAGCCAA GCTTATCGAT AAAATAAAAG ATTTTATTTA GTCTCCAGAA AAAGGGGGGA ATGAAAGACC CCACCTGTAG GTTTGGCAAG CTAGCTTAAG TAACGCCATT TTGCAAGGCA TGGAAAATAC ATAACTGAGA ATAGAGAAGT TCAGATCAAG GTTAGGAACA GAGAGACAGC AGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT CAGGGCCAAG AACAGATGGT CCCCAGATGC GGTCCCGCCC TCAGCAGTTT CTAGAGAACC ATCAGATGTT TCCAGGGTGC CCCAAGGACC TGAAATGACC CTGTGCCTTA TTTGAACTAA CCAATCAGTT CGCTTCTCGC TTCTGTTCGC GCGCTTCTGC TCCCCGAGCT CAATAAAAGA GCCCACAACC CCTCACTCGG CGCGCCAGTC CTCCGATAGA CTGCGTCGCC CGGGTACCCG TGTATCCAAT AAACCCTCTT GCAGTTGCAT CCGACTTGTG GTCTCGCTGT TCCTTGGGAG GGTCTCCTCT GAGTGATTGA CTACCCGTCA GCGGGGGTCT TTCATGGGTA ACAGTTTCTT GAAGTTGGAG AACAACATTC TGAGGGTAGG AGTCGAATAT TAAGTAATCC TGACTCAATT AGCCACTGTT TTGAATCCAC ATACTCCAAT ACTCCTGAAA TAGTTCATTA TGGACAGCGC AGAAAGAGCT GGAKAGTTCA TTATGGACAG CGCAGAAAGA GCTGGGGAGA ATTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGC GATTAGTCCA ATTTGTTAAA GACAGGATAT CAGTGGTCCA GGCTCTAGTT TTGACTCAAC AATATCACCA GCTGAAGCCT ATAGAGTACG AGCCATAGAT AAAATAAAAG ATTTTATTTA GTCTCCAGAA AAAGGGGGGA A
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