NM_004817.4;C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1;PFIC4; X104; ZO2
100μg liquid plasmid(Instantly):$120
| name | pCMV-mCherry-TJP2(human)-3×HA-Puro | another name | apCMV-mCherry-TJP2(human)-3×HA-Puro |
| Insert Size (bp) | 3570bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Red |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pCMV-mCherry-MCS-3×HA-Puro | 5′ sequencing primer | MCHERRY-F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCCAC CATGGTGAGC AAGGGCGAGG AGGATAACAT GGCCATCATC AAGGAGTTCA TGCGCTTCAA GGTGCACATG GAGGGCTCCG TGAACGGCCA CGAGTTCGAG ATCGAGGGCG AGGGCGAGGG CCGCCCCTAC GAGGGCACCC AGACCGCCAA GCTGAAGGTG ACCAAGGGTG GCCCCCTGCC CTTCGCCTGG GACATCCTGT CCCCTCAGTT CATGTACGGC TCCAAGGCCT ACGTGAAGCA CCCCGCCGAC ATCCCCGACT ACTTGAAGCT GTCCTTCCCC GAGGGCTTCA AGTGGGAGCG CGTGATGAAC TTCGAGGACG GCGGCGTGGT GACCGTGACC CAGGACTCCT CCCTGCAGGA CGGCGAGTTC ATCTACAAGG TGAAGCTGCG CGGCACCAAC TTCCCCTCCG ACGGCCCCGT AATGCAGAAG AAGACCATGG GCTGGGAGGC CTCCTCCGAG CGGATGTACC CCGAGGACGG CGCCCTGAAG GGCGAGATCA AGCAGAGGCT GAAGCTGAAG GACGGCGGCC ACTACGACGC TGAGGTCAAG ACCACCTACA AGGCCAAGAA GCCCGTGCAG CTGCCCGGCG CCTACAACGT CAACATCAAG TTGGACATCA CCTCCCACAA CGAGGACTAC ACCATCGTGG AACAGTACGA ACGCGCCGAG GGCCGCCACT CCACCGGCGG CATGGACGAG CTGTACAAGG GAGGTGGAGG TAGTGGAGGA GGCGGATCAA GCTTCATGCC GGTGCGAGGA GACCGCGGGT TTCCACCCCG GCGGGAGCTG TCAGGTTGGC TCCGCGCCCC AGGCATGGAA GAGCTGATAT GGGAACAGTA CACTGTGACC CTACAAAAGG ATTCCAAAAG AGGATTTGGA ATTGCAGTGT CCGGAGGCAG AGACAACCCC CACTTTGAAA ATGGAGAAAC GTCAATTGTC ATTTCTGATG TGCTCCCGGG TGGGCCTGCT GATGGGCTGC TCCAAGAAAA TGACAGAGTG GTCATGGTCA ATGGCACCCC CATGGAGGAT GTGCTTCATT CGTTTGCAGT TCAGCAGCTC AGAAAAAGTG GGAAGGTCGC TGCTATTGTG GTCAAGAGGC CCCGGAAGGT CCAGGTGGCC GCACTTCAGG CCAGCCCTCC CCTGGATCAG GATGACCGGG CTTTTGAGGT GATGGACGAG TTTGATGGCA GAAGTTTCCG GAGTGGCTAC AGCGAGAGGA GCCGGCTGAA CAGCCATGGG GGGCGCAGCC GCAGCTGGGA GGACAGCCCG GAAAGGGGGC GTCCCCATGA GCGGGCCCGG AGCCGGGAGC GGGACCTCAG CCGGGACCGG AGCCGTGGCC GGAGCCTGGA GCGGGGCCTG GACCAAGACC ATGCGCGCAC CCGAGACCGC AGCCGTGGCC GGAGCCTGGA GCGGGGCCTG GACCACGACT TTGGGCCATC CCGGGACCGG GACCGTGACC GCAGCCGCGG CCGGAGCATT GACCAGGACT ACGAGCGAGC CTATCACCGG GCCTACGACC CAGACTACGA GCGGGCCTAC AGCCCGGAGT ACAGGCGCGG GGCCCGCCAC GATGCCCGCT CTCGGGGACC CCGAAGCCGC AGCCGCGAGC ACCCGCACTC ACGGAGCCCC AGCCCCGAGC CTAGGGGGCG GCCGGGGCCC ATCGGGGTCC TCCTGATGAA AAGCAGAGCG AACGAAGAGT ATGGTCTCCG GCTTGGGAGT CAGATCTTCG TAAAGGAAAT GACCCGAACG GGTCTGGCAA CTAAAGATGG CAACCTTCAC GAAGGAGACA TAATTCTCAA GATCAATGGG ACTGTAACTG AGAACATGTC TTTAACGGAT GCTCGAAAAT TGATAGAAAA GTCAAGAGGA AAACTACAGC TAGTGGTGTT GAGAGACAGC CAGCAGACCC TCATCAACAT CCCGTCATTA AATGACAGTG ACTCAGAAAT AGAAGATATT TCAGAAATAG AGTCAAACCG ATCATTTTCT CCAGAGGAGA GACGTCATCA GTATTCTGAT TATGATTATC ATTCCTCAAG TGAGAAGCTG AAGGAAAGGC CAAGTTCCAG AGAGGACACG CCGAGCAGAT TGTCCAGGAT GGGTGCGACA CCCACTCCCT TTAAGTCCAC AGGGGATATT GCAGGCACAG TTGTCCCAGA GACCAACAAG GAACCCAGAT ACCAAGAGGA CCCCCCAGCT CCTCAACCAA AAGCAGCCCC GAGAACTTTT CTTCGTCCTA GTCCTGAAGA TGAAGCAATA TATGGCCCTA ATACCAAAAT GGTAAGGTTC AAGAAGGGAG ACAGCGTGGG CCTCCGGTTG GCTGGTGGCA ATGATGTCGG GATATTTGTT GCTGGCATTC AAGAAGGGAC CTCGGCGGAG CAGGAGGGCC TTCAAGAAGG AGACCAGATT CTGAAGGTGA ACACACAGGA TTTCAGAGGA TTAGTGCGGG AGGATGCCGT TCTCTACCTG TTAGAAATCC CTAAAGGTGA AATGGTGACC ATTTTAGCTC AGAGCCGAGC CGATGTGTAT AGAGACATCC TGGCTTGTGG CAGAGGGGAT TCGTTTTTTA TAAGAAGCCA CTTTGAATGT GAGAAGGAAA CTCCACAGAG CCTGGCCTTC ACCAGAGGGG AGGTCTTCCG AGTGGTAGAC ACACTGTATG ACGGCAAGCT GGGCAACTGG CTGGCTGTGA GGATTGGGAA CGAGTTGGAG AAAGGCTTAA TCCCCAACAA GAGCAGAGCT GAACAAATGG CCAGTGTTCA AAATGCCCAG AGAGACAACG CTGGGGACCG GGCAGATTTC TGGAGAATGC GTGGCCAGAG GTCTGGGGTG AAGAAGAACC TGAGGAAAAG TCGGGAAGAC CTCACAGCTG TTGTGTCTGT CAGCACCAAG TTCCCAGCTT ATGAGAGGGT TTTGCTGCGA GAAGCTGGTT TCAAGAGACC TGTGGTCTTA TTCGGCCCCA TAGCTGATAT AGCAATGGAA AAATTGGCTA ATGAGTTACC TGACTGGTTT CAAACTGCTA AAACGGAACC AAAAGATGCA GGATCTGAGA AATCCACTGG AGTGGTCCGG TTAAATACCG TGAGGCAAAT TATTGAACAG GATAAGCATG CACTACTGGA TGTGACTCCG AAAGCTGTGG ACCTGTTGAA TTACACCCAG TGGTTCCCAA TTGTGATTTT TTTCAACCCA GACTCCAGAC AAGGTGTCAA AACCATGAGA CAAAGGTTAA ATCCAACGTC CAACAAAAGT TCTCGAAAGT TATTTGATCA AGCCAACAAG CTTAAAAAAA CGTGTGCACA CCTTTTTACA GCTACAATCA ACCTAAATTC AGCCAATGAT AGCTGGTTTG GCAGCTTAAA GGACACTATT CAGCATCAGC AAGGAGAAGC GGTTTGGGTC TCTGAAGGAA AGATGGAAGG GATGGATGAT GACCCCGAAG ACCGCATGTC CTACTTAACC GCCATGGGCG CGGACTATCT GAGTTGCGAC AGCCGCCTCA TCAGTGACTT TGAAGACACG GACGGTGAAG GAGGCGCCTA CACTGACAAT GAGCTGGATG AGCCAGCCGA GGAGCCGCTG GTGTCGTCCA TCACCCGCTC CTCGGAGCCG GTGCAGCACG AGGAGAGCAT AAGGAAACCC AGCCCAGAGC CACGAGCTCA GATGAGGAGG GCTGCTAGCA GCGATCAACT TAGGGACAAT AGCCCGCCCC CAGCATTCAA GCCAGAGCCG CCCAAGGCCA AAACCCAGAA CAAAGAAGAA TCCTATGACT TCTCCAAATC CTATGAATAT AAGTCAAACC CCTCTGCCGT TGCTGGTAAT GAAACTCCTG GGGCATCTAC CAAAGGTTAT CCTCCTCCTG TTGCAGCAAA ACCTACCTTT GGGCGGTCTA TACTGAAGCC CTCCACTCCC ATCCCTCCTC AAGAGGGTGA GGAGGTGGGA GAGAGCAGTG AGGAGCAAGA TAATGCTCCC AAATCAGTCC TGGGCAAAGT CAAAATATTT GAGAAGATGG ATCACAAGGC CAGGTTACAG AGAATGCAGG AGCTCCAGGA AGCACAGAAT GCAAGGATCG AAATTGCCCA GAAGCATCCT GATATCTATG CAGTTCCAAT CAAAACGCAC AAGCCAGACC CTGGCACGCC CCAGCACACG AGTTCCAGAC CCCCTGAGCC ACAGAAAGCT CCTTCCAGAC CTTATCAGGA TACCAGAGGA AGTTATGGCA GTGATGCCGA GGAGGAGGAG TACCGCCAGC AGCTGTCAGA ACACTCCAAG CGCGGTTACT ATGGCCAGTC TGCCCGATAC CGGGACACAG AATTACTCGA GTACCCATAC GACGTACCTG ATTATGCAAC TTATCCTTAT GATGTACCTG ATTATGCTAG CTATCCTTAT GATGTCCCAG ACTACGCATA GTGAGGGCCC GTTTAAACCC GCTGATCAGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG GGATGCGGTG GGCTCTATGG CTTCTGAGGC GGAAAGAACC AGCTGGGGCT CTAGGGGGTA TCCCCACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTAATTCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGACCG AGTACAAGCC CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCCGGGCC GTACGCACCC TCGCCGCCGC GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGACCCG GACCGCCACA TCGAGCGGGT CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CGACGGATCG GGAGATCTCC CGATCCCCTA TGGT
