pBBR1MCS2-Tac-pncA(ecoli)-6×His
$55.00
In stock
SKU
PL28285
·
100μg liquid plasmid(Instantly):$98
| name | pBBR1MCS2-Tac-pncA(ecoli)-6×His | another name | apBBR1MCS2-Tac-pncA(ecoli)-6×His |
| Insert Size (bp) | Plasmid host | Wide host | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Escherichia coli | Prokaryotic resistance | Kan |
| Selective marker | Fluorescent labeling | ||
| Promoter | Tac | Replicon | pBBR1 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pBBR1MCS2-Tac-MCS | 5′ sequencing primer | |
| 3′ sequencing primer | M13R | Copy number | Low |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTCGGGCCGT CTCTTGGGCT TGATCGGCCT TCTTGCGCAT CTCACGCGCT CCTGCGGCGG CCTGTAGGGC AGGCTCATAC CCCTGCCGAA CCGCTTTTGT CAGCCGGTCG GCCACGGCTT CCGGCGTCTC AACGCGCTTT GAGATTCCCA GCTTTTCGGC CAATCCCTGC GGTGCATAGG CGCGTGGCTC GACCGCTTGC GGGCTGATGG TGACGTGGCC CACTGGTGGC CGCTCCAGGG CCTCGTAGAA CGCCTGAATG CGCGTGTGAC GTGCCTTGCT GCCCTCGATG CCCCGTTGCA GCCCTAGATC GGCCACAGCG GCCGCAAACG TGGTCTGGTC GCGGGTCATC TGCGCTTTGT TGCCGATGAA CTCCTTGGCC GACAGCCTGC CGTCCTGCGT CAGCGGCACC ACGAACGCGG TCATGTGCGG GCTGGTTTCG TCACGGTGGA TGCTGGCCGT CACGATGCGA TCCGCCCCGT ACTTGTCCGC CAGCCACTTG TGCGCCTTCT CGAAGAACGC CGCCTGCTGT TCTTGGCTGG CCGACTTCCA CCATTCCGGG CTGGCCGTCA TGACGTACTC GACCGCCAAC ACAGCGTCCT TGCGCCGCTT CTCTGGCAGC AACTCGCGCA GTCGGCCCAT CGCTTCATCG GTGCTGCTGG CCGCCCAGTG CTCGTTCTCT GGCGTCCTGC TGGCGTCAGC GTTGGGCGTC TCGCGCTCGC GGTAGGCGTG CTTGAGACTG GCCGCCACGT TGCCCATTTT CGCCAGCTTC TTGCATCGCA TGATCGCGTA TGCCGCCATG CCTGCCCCTC CCTTTTGGTG TCCAACCGGC TCGACGGGGG CAGCGCAAGG CGGTGCCTCC GGCGGGCCAC TCAATGCTTG AGTATACTCA CTAGACTTTG CTTCGCAAAG TCGTGACCGC CTACGGCGGC TGCGGCGCCC TACGGGCTTG CTCTCCGGGC TTCGCCCTGC GCGGTCGCTG CGCTCCCTTG CCAGCCCGTG GATATGTGGA CGATGGCCGC GAGCGGCCAC CGGCTGGCTC GCTTCGCTCG GCCCGTGGAC AACCCTGCTG GACAAGCTGA TGGACAGGCT GCGCCTGCCC ACGAGCTTGA CCACAGGGAT TGCCCACCGG CTACCCAGCC TTCGACCACA TACCCACCGG CTCCAACTGC GCGGCCTGCG GCCTTGCCCC ATCAATTTTT TTAATTTTCT CTGGGGAAAA GCCTCCGGCC TGCGGCCTGC GCGCTTCGCT TGCCGGTTGG ACACCAAGTG GAAGGCGGGT CAAGGCTCGC GCAGCGACCG CGCAGCGGCT TGGCCTTGAC GCGCCTGGAA CGACCCAAGC CTATGCGAGT GGGGGCAGTC GAAGGCGAAG CCCGCCCGCC TGCCCCCCGA GCCTCACGGC GGCGAGTGCG GGGGTTCCAA GGGGGCAGCG CCACCTTGGG CAAGGCCGAA GGCCGCGCAG TCGATCAACA AGCCCCGGAG GGGCCACTTT TTGCCGGAGG GGGAGCCGCG CCGAAGGCGT GGGGGAACCC CGCAGGGGTG CCCTTCTTTG GGCACCAAAG AACTAGATAT AGGGCGAAAT GCGAAAGACT TAAAAATCAA CAACTTAAAA AAGGGGGGTA CGCAACAGCT CATTGCGGCA CCCCCCGCAA TAGCTCATTG CGTAGGTTAA AGAAAATCTG TAATTGACTG CCACTTTTAC GCAACGCATA ATTGTTGTCG CGCTGCCGAA AAGTTGCAGC TGATTGCGCA TGGTGCCGCA ACCGTGCGGC ACCCTACCGC ATGGAGATAA GCATGGCCAC GCAGTCCAGA GAAATCGGCA TTCAAGCCAA GAACAAGCCC GGTCACTGGG TGCAAACGGA ACGCAAAGCG CATGAGGCGT GGGCCGGGCT TATTGCGAGG AAACCCACGG CGGCAATGCT GCTGCATCAC CTCGTGGCGC AGATGGGCCA CCAGAACGCC GTGGTGGTCA GCCAGAAGAC ACTTTCCAAG CTCATCGGAC GTTCTTTGCG GACGGTCCAA TACGCAGTCA AGGACTTGGT GGCCGAGCGC TGGATCTCCG TCGTGAAGCT CAACGGCCCC GGCACCGTGT CGGCCTACGT GGTCAATGAC CGCGTGGCGT GGGGCCAGCC CCGCGACCAG TTGCGCCTGT CGGTGTTCAG TGCCGCCGTG GTGGTTGATC ACGACGACCA GGACGAATCG CTGTTGGGGC ATGGCGACCT GCGCCGCATC CCGACCCTGT ATCCGGGCGA GCAGCAACTA CCGACCGGCC CCGGCGAGGA GCCGCCCAGC CAGCCCGGCA TTCCGGGCAT GGAACCAGAC CTGCCAGCCT TGACCGAAAC GGAGGAATGG GAACGGCGCG GGCAGCAGCG CCTGCCGATG CCCGATGAGC CGTGTTTTCT GGACGATGGC GAGCCGTTGG AGCCGCCGAC ACGGGTCACG CTGCCGCGCC GGTAGCACTT GGGTTGCGCA GCAACCCGTA AGTGCGCTGT TCCAGACTAT CGGCTGTAGC CGCCTCGCCG CCCTATACCT TGTCTGCCTC CCCGCGTTGC GTCGCGGTGC ATGGAGCCGG GCCACCTCGA CCTGAATGGA AGCCGGCGGC ACCTCGCTAA CGGATTCACC GTTTTTATCA GGCTCTGGGA GGCAGAATAA ATGATCATAT CGTCAATTAT TACCTCCACG GGGAGAGCCT GAGCAAACTG GCCTCAGGCA TTTGAGAAGC ACACGGTCAC ACTGCTTCCG GTAGTCAATA AACCGGTAGG ATCCAGTTGA CAATTAATCA TCGGCTCGTA TAATGTGGTC TAGAGTTTGT TTAACTTTAA GAAGGAGCAG GACAGTTCAA GCTTATGCCC CCTCGCGCCC TGTTACTGGT CGATTTACAA AATGATTTCT GTGCTGGTGG CGCGCTCGCC GTGCCGGAAG GTGACAGTAC GGTGGATGTC GCTAACCGCC TGATTGACTG GTGCCAGTCG CGCGGTGAAG CGGTTATCGC CAGTCAGGAC TGGCACCCGG CGAATCACGG CAGTTTTGCC AGTCAGCACG GTGTAGAGCC TTATACGCCA GGCCAACTCG ACGGTTTGCC ACAAACCTTC TGGCCAGATC ACTGTGTGCA GAACAGTGAA GGCGCACAAT TACATCCGTT ACTGCACCAA AAAGCGATCG CAGCGGTGTT CCATAAAGGC GAAAATCCTT TAGTTGACAG TTACAGTGCC TTTTTTGATA ACGGCCGTCG GCAGAAAACC TCTCTCGATG ACTGGTTACG CGATCATGAA ATCGATGAAT TGATCGTTAT GGGCCTGGCT ACTGACTATT GCGTGAAGTT TACCGTGCTG GACGCGTTAC AGTTAGGTTA TAAGGTAAAC GTGATTACCG ATGGTTGTCG TGGCGTGAAT ATCCAGCCCC AGGACAGTGC GCACGCGTTT ATGGAGATGT CAGCAGCTGG GGCAACGCTA TATACGCTGG CAGACTGGGA AGAGACACAG GGGCACCATC ACCATCACCA TTAACTCGAG GATCGATACG TCGACGAGAA GGCCATCCTG ACGGATGGCC TTTTGGTACC CAGCTTTTGT TCCCTTTAGT GAGGGTTAAT TGCGCGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CATGCATAAA AACTGTTGTA ATTCATTAAG CATTCTGCCG ACATGGAAGC CATCACAAAC GGCATGATGA ACCTGAATCG CCAGCGGCAT CAGCACCTTG TCGCCTTGCG TATAATATTT GCCCATGGGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGGTC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCGCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCCTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCAGATC TTGATCCCCT GCGCCATCAG ATCCTTGGCG GCAAGAAAGC CATCCAGTTT ACTTTGCAGG GCTTCCCAAC CTTACCAGAG GGCGCCCCAG CTGGCAATTC CGGTTCGCTT GCTGTCCATA AAACCGCCCA GTCTAGCTAT CGCCATGTAA GCCCACTGCA AGCTACCTGC TTTCTCTTTG CGCTTGCGTT TTCCCTTGTC CAGATAGCCC AGTAGCTGAC ATTCATCCCA GGTGGCACTT TTCGGGGAAA TGTGCGCGCC CGCGTTCCTG CTGGCGCTGG GCCTGTTTCT GGCGCTGGAC TTCCCGCTGT TCCGTCAGCA GCTTTTCGCC CACGGCCTTG ATGATCGCGG CGGCCTTGGC CTGCATATCC CGATTCAACG GCCCCAGGGC GTCCAGAACG GGCTTCAGGC GCTCCCGAAG GT
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