pCMV-Gm51500(mouse)-lncRNA-Neo
$55.00
In stock
SKU
PL28269
XR_003946886.1
100μg liquid plasmid(Instantly):$76
| name | pCMV-Gm51500(mouse)-lncRNA-Neo | another name | apCMV-Gm51500(mouse)-lncRNA-Neo |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Transcription regulation | Fragment type | ncRNA |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pCDNA3.1(+) | 5′ sequencing primer | CMV-F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC CTTAAAAGAT GTGTGAGGGG CTGGCATCCT CGATGGGGCT GTGCATGTTC CCAGTCTTGT TGGTTGGAAG GCTGAGTTAT CAGGACATCC TTCCGGCCTT CTACCAGAAG GTAGCAACAG GTTTTAACTA CCCCAAGTCT ACTTCTTCTA TATTACCATA CTTCTCAGAA AGAGACAGAT CCTTTTCTAC TAATGTTTGC TATTTTACTA GCCAGCAGAG AAGCAGCAGA GGAGAAGAGA AGACCTGAAT GCCAGGTCTC CGAATCAGAA AGAATGATGA AGCCATTACA GAACCTCCTG CAAGGACCAA ACGGTTATTC CTGGGCTGTG GGGAGCAGAT GGGGACTCTT GGTCATCATG AGCTGGGATC AACGTAGCTC TTGCCTCTCA TCAAAGACAT TCTTTGCAAC AGATGGGGAC CATTACAGAA ACCATGACTG GTCAAAAAGC TGACTACAAG CAATCATAGG GCCATCAGAC CCTGTACCCT GTACCCTGTA CCCTGTACCC TGTACCCTGA GATCAGGGAA CATTTCAGAA GAGGGGACAG GAAGATGTTA AGGGTCAGAG GACCAGAAAA CCATCAGTGA GACTATGACT CCTAGAAGCA TCAGGGAAGC TACATCCATG GTACCTCAAT AATGTGGCTG CTTAGCTAAG ACCTGAACAA GGTTGATAAC AGTTAACATG CTGACAAGAA ATGGGGAATC TCAAGGGGAT CCATCTCTAG ACCAAGAACT ATGGGCAACT AAGGGATGCT GAGCATGACA ATAGTCTTCT CCAGGCAAGA TTATACATAC CAAACACAAA ACCCCAGACC AGAAAACCCA ACAACTACAA CCAAACTCCA AACTCACATA TTCACTGAGC AGAAATGACA TCACTGTAAA GAGCTACTCA ACAGCTCCTG GAAAATAAGA TGCTGGGAGC TTCCAACTGA CATTTTATTT GCAGACTGAG ATGGAAGCCA CAGCACAGCA GATCTGCTTC CCGCAGGATA GCCTGATCAC TCCAGATCAG AGGGAGGCAT GGACGGAGCC CATTCTGTCA CCTGATGCTA TATTGGCTGG ACACCAAGAA CACTGGCTCA GAGGATGGAC CAGACCTTAG TGAAGACCAT GGGAACCGCA TCTTTATATT CTTGCAAGAA CAGCAGTGAC TTCACCAGAA CCAATGCTGA CCCAGTCAAT GGGCCTGCTA GATGGGCTAC TGAAGGCTAA GGAAGATGCA GGCTACGGTG AAGACTCTAG CATGTCCTCG CCTCGGTAGT ACATGGTCGT GGTTAATAGA AAGCACCTAG GGCACAGAAG AATCCATCTG CTGAACATAA TTGTTCATGG AGCTAAACAT GTGAGCTCTG ACCAATGTTC CTCACTGGAA CCTAATTAAA GTGGGGATCT AATTCCCCAG GAATTTGAAT AGAGGGACAC TAAGTGAGTT GTGGTGAGTT AAATCATACA CAGGAAGCTT CTGGATGCTG CCACCGTCTC ACTTCTACAC AGAATAGCTG TTCCTTCCAG AAGTGAGGTC CTGGAAGCCA AGGACCCAGT GGCAAGGAAC ACAGTGTTCA GAGGCTCCCA TTGGTTCGGC AGAGGCTCTG TCTGTGTCAG ATGAGCAGCT TAACATTCGG TTAAATGGAA AAGCTTCTGC GTGTGGGGCT CTCCAGGGCT GGGTGCTGAC AGACATGAAC CGGTGCTGTG CTTAATGAGG GACAATTTAT ACGCCTCATG TAATAGACTC CTTTCTGCTG TGGTAGCAGA CACACCTGCA CATACCATGC TTCTCAGGAG AAAGGAGCTT CGCGTCAGAC CTGCTAGGTA AGGCAGCCAT AACAGAACTC TGAACCCCCA GCTATGGCAA AAATCAGTGC ACATCTCGTC CTGGTCCCGC TAGTTGTCTC TTGCATGGGT TCCCACCAGG GAGTACAGGA ATGGTGAGAG ACTCTCTATT TCATCCCCAA GTCCCGAGAG CTTGAGGCCC TAAAGCTCGC TGGCACCTGG TGAAGGGCAA AGGACTGGGC TTGGAGCCAG GAGCTTGGGC CTCTGTCCTC CCTGACCTGC AGTCTTCTCA CTTATCAGAG GGAGCTGAGT GTGTCTGCAT TCTCAGGGCT GTGACAAGCA GCATGAGAAA GACGCCAGAG TTCTTTGTTC AGTGGAGTGA GGTCTAGTTG GGGTGATGGG GTTTTGGTTA TACGTTCTAG AACTGTTAGA TTGTACATGG TTTTTTTGGC ATACCTCCAG GAAAAGCTCC ACTGGGAAGA CCCTCTGCAT TTGCAAAGCA AGTTTCTCTC ATCTCCTCGC TCTCCATGGA GTGCATACAG AATAGCAACA GGCTTGGCCT TTTCATCCTG GGTGTTTACT CAACTCCTAC TTTCAGTTTT TGTAAGTTTT TCTACTGATT GTATCGAGTC CATTCTTGTA AGTGGTTGTA ATCCTGCTTA TAAAGTTGTG GTGAAAAAAA AAAAAAACAG GCGTGATGGT GCATACCTTT AATCCCAGCA CTTGGGAGGC AGAGGCAGGT GGATTTCTGA GTTCGAGGCC AGCCTGGTCT ACAAAGTGAG TTCCAGGAAA GCCAGGGCTA TACAGAGAAA CCCTGTCTCA ACAAACAAAC AAACAAACAA AAAACAACAA AAAACAAAAC AAAACAAAAC AAACAAACAA ACAAAAAAGA TGTGGTGCAC ACAGAAAAGG AAAATGGAGG ATGGACAAAT GCTAAATTTG ATAAAATGAT GATGGTCATA CAGGTTTTCC TCTAGAGCTT TAGTCATTAA AATAGGAAAC AAATGATTGT GTAGTAATTG CCACTTATCC ATTCATCTAC ATATGCAATT ATTATCTGCC GCCCACCCAT CCATCATCCA TCTACCTATC TACCAACCTG TCAGTCTAAC TATCCATTCA TTCCCCTATC CACCACCCAA CCATCCACAA ATCTCCAACC CACCCATCTT TTCACCATCA CTCCAACCCC CTATCCATCT ATTCATCTAC CCACCTATCT ACTCACCCAC TCACCATCTA CCCCATCCCT ACCTGCTCAC TTTCTCCCTA CTCACCCAAC TACCCATCTA TGCATCAGTT TATATCTATG CACTTACCCC CAACCCATCA ATCTGCTCAC CAACACCTCG AGTCTAGAGG GCCCGTTTAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG AACCAGCTGG GGCTCTAGGG GGTATCCCCA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTAA TTCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC TGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC GACGGATCGG GAGATCTCCC GATCCCCTAT GGT
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