pSEVA241
$55.00
In stock
SKU
PL28262
·
100μg liquid plasmid(Instantly):$98
| name | pSEVA241 | another name | apSEVA241 |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Fluorescent labeling | ||
| Promoter | pRO1600 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | rrnBT1-R | Copy number | Low |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TTAATTAAAG CGGATAACAA TTTCACACAG GAGGCCGCCT AGGCCGCGGC CGCGCGAATT CGAGCTCGGT ACCCGGGGAT CCTCTAGAGT CGACCTGCAG GCATGCAAGC TTGCGGCCGC GTCGTGACTG GGAAAACCCT GGCGACTAGT CTTGGACTCC TGTTGATAGA TCCAGTAATG ACCTCAGAAC TCCATCTGGA TTTGTTCAGA ACGCTCGGTT GCCGCCGGGC GTTTTTTATT GGTGAGAATC CAGGGGTCCC CAATAATTAC GATTTAAATT TGTGTCTCAA AATCTCTGAT GTTACATTGC ACAAGATAAA AATATATCAT CATGAACAAT AAAACTGTCT GCTTACATAA ACAGTAATAC AAGGGGTGTT ATGAGCCATA TTCAGCGTGA AACGAGCTGT AGCCGTCCGC GTCTGAACAG CAACATGGAT GCGGATCTGT ATGGCTATAA ATGGGCGCGT GATAACGTGG GTCAGAGCGG CGCGACCATT TATCGTCTGT ATGGCAAACC GGATGCGCCG GAACTGTTTC TGAAACATGG CAAAGGCAGC GTGGCGAACG ATGTGACCGA TGAAATGGTG CGTCTGAACT GGCTGACCGA ATTTATGCCG CTGCCGACCA TTAAACATTT TATTCGCACC CCGGATGATG CGTGGCTGCT GACCACCGCG ATTCCGGGCA AAACCGCGTT TCAGGTGCTG GAAGAATATC CGGATAGCGG CGAAAACATT GTGGATGCGC TGGCCGTGTT TCTGCGTCGT CTGCATAGCA TTCCGGTGTG CAACTGCCCG TTTAACAGCG ATCGTGTGTT TCGTCTGGCC CAGGCGCAGA GCCGTATGAA CAACGGCCTG GTGGATGCGA GCGATTTTGA TGATGAACGT AACGGCTGGC CGGTGGAACA GGTGTGGAAA GAAATGCATA AACTGCTGCC GTTTAGCCCG GATAGCGTGG TGACCCACGG CGATTTTAGC CTGGATAACC TGATTTTCGA TGAAGGCAAA CTGATTGGCT GCATTGATGT GGGCCGTGTG GGCATTGCGG ATCGTTATCA GGATCTGGCC ATTCTGTGGA ACTGCCTGGG CGAATTTAGC CCGAGCCTGC AAAAACGTCT GTTTCAGAAA TATGGCATTG ATAATCCGGA TATGAACAAA CTGCAATTTC ATCTGATGCT GGATGAATTT TTCTAATAAT TAATTGGACC GCGGTCCGCG CGTTGTCCTT TTCCGCTGCA TAACCCTGCT TCGGGGTCAT TATAGCGATT TTTTCGGTAT ATCCATCCTT TTTCGCACGA TATACAGGAT TTTGCCAAAG GGTTCGTGTA GACTTTCCTT GGTGTATCCA ACGGCGTCAG CCGGGCAGGA TAGGTGAAGT AGGCCCACCC GCGAGCGGGT GTTCCTTCTT CACTGTCCCT TATTCGCACC TGGCGGTGCT CAACGGGAAT CCTGCTCTGC GAGGCTGGCC GTAGGCCGGC CGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG CATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CGTGAAAGGC AGGCCGGTCC GTGGTGGCCA CGGCCTCTAG GCCAGATCCA GCGGCATCTG GGTTAGTCGA GCGCGGGCCG CTTCCCATGT CTCACCAGGG CGAGCCTGTT TCGCGATCTC AGCATCTGAA ATCTTCCCGG CCTTGCGCTT CGCTGGGGCC TTACCCACCG CCTTGGCGGG CTTCTTCGGT CCAAAACTGA ACAACAGATG TGTGACCTTG CGCCCGGTCT TTCGCTGCGC CCACTCCACC TGTAGCGGGC TGTGCTCGTT GATCTGCGTC ACGGCTGGAT CAAGCACTCG CAACTTGAAG TCCTTGATCG AGGGATACCG GCCTTCCAGT TGAAACCACT TTCGCAGCTG GTCAATTTCT ATTTCGCGCT GGCCGATGCT GTCCCATTGC ATGAGCAGCT CGTAAAGCCT GATCGCGTGG GTGCTGTCCA TCTTGGCCAC GTCAGCCAAG GCGTATTTGG TGAACTGTTT GGTGAGTTCC GTCAGGTACG GCAGCATGTC TTTGGTGAAC CTGAGTTCTA CACGGCCCTC ACCCTCCCGG TAGATGATTG TTTGCACCCA GCCGGTAATC ATCACACTCG GTCTTTTCCC CTTGCCATTG GGCTCTTGGG TTAACCGGAC TTCCCGCCGT TTCAGGCGCA GGGCCGCTTC TTTGAGCTGG TTGTAGGAAG ATTCGATAGG GACACCCGCC ATCGTCGCTA TGTCCTCCGC CGTCACTGAA TACATCACTT CATCGGTGAC AGGCTCGCTC CTCTTCACCT GGCTAATACA GGCCAGAACG ATCCGCTGTT CCTGAACACT GAGGCGATAC GCGGCCTCGA CCAGGGCATT GCTTTTGTAA ACCATTGGGG GTGAGGCCAC GTTCGACATT CCTTGTGTAT AAGGGGACAC TGTATCTGCG TCCCACAATA CAACAAATCC GTCCCTTTAC AACAACAAAT CCGTCCCTTC TTAACAACAA ATCCGTCCCT TAATGGCAAC AAATCCGTCC CTTTTTAAAC TCTACAGGCC ACGGATTACG TGGCCTGTAG ACGTCCTAAA AGGTTTAAAA GGGAAAAGGA AGAAAAGGGT GGAAACGCAA AAAACGCACC ACTACGTGGC CCCGTTGGGG CCGCATTTGT GCCCCTGAAG GGGCGGGGGA GGCGTCTGGG CAATCCCCGT TTTACCAGTC CCCTATCGCC GCCTGAGAGG GCGCAGGAAG CGAGTAATCA GGGTATCGAG GCGGATTCAC CCTTGGCGTC CAACCAGCGG CACCAGCGGC GCCTGAGAGG GGCGCGCCCA GCTGTCTAGG GCGGCGGATT TGTCCTACTC AGGAGAGCGT TCACCGACAA ACAACAGATA AAACGAAAGG CCCAGTCTTT CGACTGAGCC TTTCGTTTTA TTTGATGCCT
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