pLV3-CMV-CEMIP(human)-3×FLAG-Puro
$113.00
In stock
SKU
PL28249
NM_001293298.2;CCSP1; HYBID; KIAA1199; TMEM2L
100μg liquid plasmid(Instantly):$134
| name | pLV3-CMV-CEMIP(human)-3×FLAG-Puro | another name | apLV3-CMV-CEMIP(human)-3×FLAG-Puro |
| Insert Size (bp) | 4086bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | pLV3-CMV-MCS-3×FLAG-Puro | 5′ sequencing primer | CMV-F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT AGAGCTAGCG AATTCGCCAC CATGGGAGCT GCTGGGAGGC AGGACTTCCT CTTCAAGGCC ATGCTGACCA TCAGCTGGCT CACTCTGACC TGCTTCCCTG GGGCCACATC CACAGTGGCT GCTGGGTGCC CTGACCAGAG CCCTGAGTTG CAACCCTGGA ACCCTGGCCA TGACCAAGAC CACCATGTGC ATATCGGCCA GGGCAAGACA CTGCTGCTCA CCTCTTCTGC CACGGTCTAT TCCATCCACA TCTCAGAGGG AGGCAAGCTG GTCATTAAAG ACCACGACGA GCCGATTGTT TTGCGAACCC GGCACATCCT GATTGACAAC GGAGGAGAGC TGCATGCTGG GAGTGCCCTC TGCCCTTTCC AGGGCAATTT CACCATCATT TTGTATGGAA GGGCTGATGA AGGTATTCAG CCGGATCCTT ACTATGGTCT GAAGTACATT GGGGTTGGTA AAGGAGGCGC TCTTGAGTTG CATGGACAGA AAAAGCTCTC CTGGACATTT CTGAACAAGA CCCTTCACCC AGGTGGCATG GCAGAAGGAG GCTATTTTTT TGAAAGGAGC TGGGGCCACC GTGGAGTTAT TGTTCATGTC ATCGACCCCA AATCAGGCAC AGTCATCCAT TCTGACCGGT TTGACACCTA TAGATCCAAG AAAGAGAGTG AACGTCTGGT CCAGTATTTG AACGCGGTGC CCGATGGCAG GATCCTTTCT GTTGCAGTGA ATGATGAAGG TTCTCGAAAT CTGGATGACA TGGCCAGGAA GGCGATGACC AAATTGGGAA GCAAACACTT CCTGCACCTT GGATTTAGAC ACCCTTGGAG TTTTCTAACT GTGAAAGGAA ATCCATCATC TTCAGTGGAA GACCATATTG AATATCATGG ACATCGAGGC TCTGCTGCTG CCCGGGTATT CAAATTGTTC CAGACAGAGC ATGGCGAATA TTTCAATGTT TCTTTGTCCA GTGAGTGGGT TCAAGACGTG GAGTGGACGG AGTGGTTCGA TCATGATAAA GTATCTCAGA CTAAAGGTGG GGAGAAAATT TCAGACCTCT GGAAAGCTCA CCCAGGAAAA ATATGCAATC GTCCCATTGA TATACAGGCC ACTACAATGG ATGGAGTTAA CCTCAGCACC GAGGTTGTCT ACAAAAAAGG CCAGGATTAT AGGTTTGCTT GCTACGACCG GGGCAGAGCC TGCCGGAGCT ACCGTGTACG GTTCCTCTGT GGGAAGCCTG TGAGGCCCAA ACTCACAGTC ACCATTGACA CCAATGTGAA CAGCACCATT CTGAACTTGG AGGATAATGT ACAGTCATGG AAACCTGGAG ATACCCTGGT CATTGCCAGT ACTGATTACT CCATGTACCA GGCAGAAGAG TTCCAGGTGC TTCCCTGCAG ATCCTGCGCC CCCAACCAGG TCAAAGTGGC AGGGAAACCA ATGTACCTGC ACATCGGGGA GGAGATAGAC GGCGTGGACA TGCGGGCGGA GGTTGGGCTT CTGAGCCGGA ACATCATAGT GATGGGGGAG ATGGAGGACA AATGCTACCC CTACAGAAAC CACATCTGCA ATTTCTTTGA CTTCGATACC TTTGGGGGCC ACATCAAGTT TGCTCTGGGA TTTAAGGCAG CACACTTGGA GGGCACGGAG CTGAAGCATA TGGGACAGCA GCTGGTGGGT CAGTACCCGA TTCACTTCCA CCTGGCCGGT GATGTAGACG AAAGGGGAGG TTATGACCCA CCCACATACA TCAGGGACCT CTCCATCCAT CATACATTCT CTCGCTGCGT CACAGTCCAT GGCTCCAATG GCTTGTTGAT CAAGGACGTT GTGGGCTATA ACTCTTTGGG CCACTGCTTC TTCACGGAAG ATGGGCCGGA GGAACGCAAC ACTTTTGACC ACTGTCTTGG CCTCCTTGTC AAGTCTGGAA CCCTCCTCCC CTCGGACCGT GACAGCAAGA TGTGCAAGAT GATCACAGAG GACTCCTACC CGGGGTACAT CCCCAAGCCC AGGCAAGACT GCAATGCTGT GTCCACCTTC TGGATGGCCA ATCCCAACAA CAACCTCATC AACTGTGCCG CTGCAGGATC TGAGGAAACT GGATTTTGGT TTATTTTTCA CCACGTACCA ACGGGCCCCT CCGTGGGAAT GTACTCCCCA GGTTATTCAG AGCACATTCC ACTGGGAAAA TTCTATAACA ACCGAGCACA TTCCAACTAC CGGGCTGGCA TGATCATAGA CAACGGAGTC AAAACCACCG AGGCCTCTGC CAAGGACAAG CGGCCGTTCC TCTCAATCAT CTCTGCCAGA TACAGCCCTC ACCAGGACGC CGACCCGCTG AAGCCCCGGG AGCCGGCCAT CATCAGACAC TTCATTGCCT ACAAGAACCA GGACCACGGG GCCTGGCTGC GCGGCGGGGA TGTGTGGCTG GACAGCTGCC GGTTTGCTGA CAATGGCATT GGCCTGACCC TGGCCAGTGG TGGAACCTTC CCGTATGACG ACGGCTCCAA GCAAGAGATA AAGAACAGCT TGTTTGTTGG CGAGAGTGGC AACGTGGGGA CGGAAATGAT GGACAATAGG ATCTGGGGCC CTGGCGGCTT GGACCATAGC GGAAGGACCC TCCCTATAGG CCAGAATTTT CCAATTAGAG GAATTCAGTT ATATGATGGC CCCATCAACA TCCAAAACTG CACTTTCCGA AAGTTTGTGG CCCTGGAGGG CCGGCACACC AGCGCCCTGG CCTTCCGCCT GAATAATGCC TGGCAGAGCT GCCCCCATAA CAACGTGACC GGCATTGCCT TTGAGGACGT TCCGATTACT TCCAGAGTGT TCTTCGGAGA GCCTGGGCCC TGGTTCAACC AGCTGGACAT GGATGGGGAT AAGACATCTG TGTTCCATGA CGTCGACGGC TCCGTGTCCG AGTACCCTGG CTCCTACCTC ACGAAGAATG ACAACTGGCT GGTCCGGCAC CCAGACTGCA TCAATGTTCC CGACTGGAGA GGGGCCATTT GCAGTGGGTG CTATGCACAG ATGTACATTC AAGCCTACAA GACCAGTAAC CTGCGAATGA AGATCATCAA GAATGACTTC CCCAGCCACC CTCTTTACCT GGAGGGGGCG CTCACCAGGA GCACCCATTA CCAGCAATAC CAACCGGTTG TCACCCTGCA GAAGGGCTAC ACCATCCACT GGGACCAGAC GGCCCCCGCC GAACTCGCCA TCTGGCTCAT CAACTTCAAC AAGGGCGACT GGATCCGAGT GGGGCTCTGC TACCCGCGAG GCACCACATT CTCCATCCTC TCGGATGTTC ACAATCGCCT GCTGAAGCAA ACGTCCAAGA CGGGCGTCTT CGTGAGGACC TTGCAGATGG ACAAAGTGGA GCAGAGCTAC CCTGGCAGGA GCCACTACTA CTGGGACGAG GACTCAGGGC TGTTGTTCCT GAAGCTGAAA GCTCAGAACG AGAGAGAGAA GTTTGCTTTC TGCTCCATGA AAGGCTGTGA GAGGATAAAG ATTAAAGCTC TGATTCCAAA GAACGCAGGC GTCAGTGACT GCACAGCCAC AGCTTACCCC AAGTTCACCG AGAGGGCTGT CGTAGACGTG CCGATGCCCA AGAAGCTCTT TGGTTCTCAG CTGAAAACAA AGGACCATTT CTTGGAGGTG AAGATGGAGA GTTCCAAGCA GCACTTCTTC CACCTCTGGA ACGACTTCGC TTACATTGAA GTGGATGGGA AGAAGTACCC CAGTTCGGAG GATGGCATCC AGGTGGTGGT GATTGACGGG AACCAAGGGC GCGTGGTGAG CCACACGAGC TTCAGGAACT CCATTCTGCA AGGCATACCA TGGCAGCTTT TCAACTATGT GGCGACCATC CCTGACAATT CCATAGTGCT TATGGCATCA AAGGGAAGAT ACGTCTCCAG AGGCCCATGG ACCAGAGTGC TGGAAAAGCT TGGGGCAGAC AGGGGTCTCA AGTTGAAAGA GCAAATGGCA TTCGTTGGCT TCAAAGGCAG CTTCCGGCCC ATCTGGGTGA CACTGGACAC TGAGGATCAC AAAGCCAAAA TCTTCCAAGT TGTGCCCATC CCTGTGGTGA AGAAGAAGAA GTTGGGATCC GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT GACAAGTAGC GGCCGCGAAG GATCTGCGAT CGCTCCGGTG CCCGTCAGTG GGCAGAGCGC ACATCGCCCA CAGTCCCCGA GAAGTTGGGG GGAGGGGTCG GCAATTGAAC GGGTGCCTAG AGAAGGTGGC GCGGGGTAAA CTGGGAAAGT GATGTCGTGT ACTGGCTCCG CCTTTTTCCC GAGGGTGGGG GAGAACCGTA TATAAGTGCA GTAGTCGCCG TGAACGTTCT TTTTCGCAAC GGGTTTGCCG CCAGAACACA GCTGAAGCTT CGAGGGGCTC GCATCTCTCC TTCACGCGCC CGCCGCCCTA CCTGAGGCCG CCATCCACGC CGGTTGAGTC GCGTTCTGCC GCCTCCCGCC TGTGGTGCCT CCTGAACTGC GTCCGCCGTC TAGGTAAGTT TAAAGCTCAG GTCGAGACCG GGCCTTTGTC CGGCGCTCCC TTGGAGCCTA CCTAGACTCA GCCGGCTCTC CACGCTTTGC CTGACCCTGC TTGCTCAACT CTACGTCTTT GTTTCGTTTT CTGTTCTGCG CCGTTACAGA TCCAAGCTGT GACCGGCGCC TACGCTAGAT GACCGAGTAC AAGCCCACGG TGCGCCTCGC CACCCGCGAC GACGTCCCCC GGGCCGTACG CACCCTCGCC GCCGCGTTCG CCGACTACCC CGCCACGCGC CACACCGTCG ACCCGGACCG CCACATCGAG CGGGTCACCG AGCTGCAAGA ACTCTTCCTC ACGCGCGTCG GGCTCGACAT CGGCAAGGTG TGGGTCGCGG ACGACGGCGC CGCGGTGGCG GTCTGGACCA CGCCGGAGAG CGTCGAAGCG GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC GCGCAGCAAC AGATGGAAGG CCTCCTGGCG CCGCACCGGC CCAAGGAGCC CGCGTGGTTC CTGGCCACCG TCGGCGTCTC GCCCGACCAC CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG CTCCCCGGAG TGGAGGCGGC CGAGCGCGCC GGGGTGCCCG CCTTCCTGGA GACCTCCGCG CCCCGCAACC TCCCCTTCTA CGAGCGGCTC GGCTTCACCG TCACCGCCGA CGTCGAGGTG CCCGAAGGAC CGCGCACCTG GTGCATGACC CGCAAGCCCG GTGCCTGAGT CGACAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAA TCATCGTCCT TTCCTTGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCCTGGTAC CTTTAAGACC AATGACTTAC AAGGCAGCTG TAGATCTTAG CCACTTTTTA AAAGAAAAGG GGGGACTGGA AGGGCTAATT CACTCCCAAC GAAAATAAGA TCTGCTTTTT GCTTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GTAGTAGTTC ATGTCATCTT ATTATTCAGT ATTTATAACT TGCAAAGAAA TGAATATCAG AGAGTGAGAG GAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGCTCTAGC TATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG ACTTTTGCAG AGACGGCCCA AATTCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCTAAGAAAC CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGCCAAGCTG
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