pCMV-AGRN(human)-TM2-P2A-mCherry-Neo
$142.00
In stock
SKU
PL28216
NM_001305275.2;AGRIN; CMS8; CMSPPD
100μg liquid plasmid(Instantly):$163
| name | pCMV-AGRN(human)-TM2-P2A-mCherry-Neo | another name | apCMV-AGRN(human)-TM2-P2A-mCherry-Neo |
| Insert Size (bp) | 6207bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | Red |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pCMV-GDA(human)-P2A-mCherry-Neo | 5′ sequencing primer | CMV-F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGACACC GGGACCGATC CAGCCTCCGG ACTCTAGAGC TAGCGTTTAA ACTTAAGCTT GGTACCGAGC TCGGATCCGC CACCATGGCC GGCCGGTCCC ACCCGGGCCC GCTGCGGCCG CTGCTGCCGC TCCTTGTGGT GGCCGCGTGC GTCCTGCCCG GAGCCGGCGG GACATGCCCG GAGCGCGCGC TGGAGCGGCG CGAGGAGGAG GCGAACGTGG TGCTCACCGG GACGGTGGAG GAGATCCTCA ACGTGGACCC GGTGCAGCAC ACGTACTCCT GCAAGGTTCG GGTCTGGCGG TACTTGAAGG GCAAAGACCT GGTGGCCCGG GAGAGCCTGC TGGACGGCGG CAACAAGGTG GTGATCAGCG GCTTTGGAGA CCCCCTCATC TGTGACAACC AGGTGTCCAC TGGGGACACC AGGATCTTCT TTGTGAACCC TGCACCCCCA TACCTGTGGC CAGCCCACAA GAACGAGCTG ATGCTCAACT CCAGCCTCAT GCGGATCACC CTGCGGAACC TGGAGGAGGT GGAGTTCTGT GTGGAAGATA AACCCGGGAC CCACTTCACT CCAGTGCCTC CGACGCCTCC TGATGCGTGC CGGGGAATGC TGTGCGGCTT CGGCGCCGTG TGCGAGCCCA ACGCGGAGGG GCCGGGCCGG GCGTCCTGCG TCTGCAAGAA GAGCCCGTGC CCCAGCGTGG TGGCGCCTGT GTGTGGGTCG GACGCCTCCA CCTACAGCAA CGAATGCGAG CTGCAGCGGG CGCAGTGCAG CCAGCAGCGC CGCATCCGCC TGCTCAGCCG CGGGCCGTGC GGCTCGCGGG ACCCCTGCTC CAACGTGACC TGCAGCTTCG GCAGCACCTG TGCGCGCTCG GCCGACGGGC TGACGGCCTC GTGCCTGTGC CCCGCGACCT GCCGTGGCGC CCCCGAGGGG ACCGTCTGCG GCAGCGACGG CGCCGACTAC CCCGGCGAGT GCCAGCTCCT GCGCCGCGCC TGCGCCCGCC AGGAGAATGT CTTCAAGAAG TTCGACGGCC CTTGTGACCC CTGTCAGGGC GCCCTCCCTG ACCCGAGCCG CAGCTGCCGT GTGAACCCGC GCACGCGGCG CCCTGAGATG CTCCTACGGC CCGAGAGCTG CCCTGCCCGG CAGGCGCCAG TGTGTGGGGA CGACGGAGTC ACCTACGAAA ACGACTGTGT CATGGGCCGA TCGGGGGCCG CCCGGGGTCT CCTCCTGCAG AAAGTGCGCT CCGGCCAGTG CCAGGGTCGA GACCAGTGCC CGGAGCCCTG CCGGTTCAAT GCCGTGTGCC TGTCCCGCCG TGGCCGTCCC CGCTGCTCCT GCGACCGCGT CACCTGTGAC GGGGCCTACA GGCCCGTGTG TGCCCAGGAC GGGCGCACGT ATGACAGTGA TTGCTGGCGG CAGCAGGCTG AGTGCCGGCA GCAGCGTGCC ATCCCCAGCA AGCACCAGGG CCCGTGTGAC CAGGCCCCGT CCCCATGCCT CGGGGTGCAG TGTGCATTTG GGGCGACGTG TGCTGTGAAG AACGGGCAGG CAGCGTGTGA ATGCCTGCAG GCGTGCTCGA GCCTCTACGA TCCTGTGTGC GGCAGCGACG GCGTCACATA CGGCAGCGCG TGCGAGCTGG AGGCCACGGC CTGTACCCTC GGGCGGGAGA TCCAGGTGGC GCGCAAAGGA CCCTGTGACC GCTGCGGGCA GTGCCGCTTT GGAGCCCTGT GCGAGGCCGA GACCGGGCGC TGCGTGTGCC CCTCTGAATG CGTGGCTTTG GCCCAGCCCG TGTGTGGCTC CGACGGGCAC ACGTACCCCA GCGAGTGCAT GCTGCACGTG CACGCCTGCA CACACCAGAT CAGCCTGCAC GTGGCCTCAG CTGGACCCTG TGAGACCTGT GGAGATGCCG TGTGTGCTTT TGGGGCTGTG TGCTCCGCAG GGCAGTGTGT GTGTCCCCGG TGTGAGCACC CCCCGCCCGG CCCCGTGTGT GGCAGCGACG GTGTCACCTA CGGCAGTGCC TGCGAGCTAC GGGAAGCCGC CTGCCTCCAG CAGACACAGA TCGAGGAGGC CCGGGCAGGG CCGTGCGAGC AGGCCGAGTG CGGTTCCGGA GGCTCTGGCT CTGGGGAGGA CGGTGACTGT GAGCAGGAGC TGTGCCGGCA GCGCGGTGGC ATCTGGGACG AGGACTCGGA GGACGGGCCG TGTGTCTGTG ACTTCAGCTG CCAGAGTGTC CCAGGCAGCC CGGTGTGCGG CTCAGATGGG GTCACCTACA GCACCGAGTG TGAGCTGAAG AAGGCCAGGT GTGAGTCACA GCGAGGGCTC TACGTAGCGG CCCAGGGAGC CTGCCGAGGC CCCACCTTCG CCCCGCTGCC GCCTGTGGCC CCCTTACACT GTGCCCAGAC GCCCTACGGC TGCTGCCAGG ACAATATCAC CGCAGCCCGG GGCGTGGGCC TGGCTGGCTG CCCCAGTGCC TGCCAGTGCA ACCCCCATGG CTCTTACGGC GGCACCTGTG ACCCAGCCAC AGGCCAGTGC TCCTGCCGCC CAGGTGTGGG GGGCCTCAGG TGTGACCGCT GTGAGCCTGG CTTCTGGAAC TTTCGAGGCA TCGTCACCGA TGGCCGGAGT GGCTGTACAC CCTGCAGCTG TGATCCCCAA GGCGCCGTGC GGGATGACTG TGAGCAGATG ACGGGGCTGT GCTCGTGTAA GCCCGGGGTG GCTGGACCCA AGTGTGGGCA GTGTCCAGAC GGCCGTGCCC TGGGCCCCGC GGGCTGTGAA GCTGACGCTT CTGCGCCTGC GACCTGTGCG GAGATGCGCT GTGAGTTCGG TGCGCGGTGC GTGGAGGAGT CTGGCTCAGC CCACTGTGTC TGCCCGATGC TCACCTGTCC AGAGGCCAAC GCTACCAAGG TCTGTGGGTC AGATGGAGTC ACATACGGCA ACGAGTGTCA GCTGAAGACC ATCGCCTGCC GCCAGGGCCT GCAAATCTCT ATCCAGAGCC TGGGCCCGTG CCAGGAGGCT GTTGCTCCCA GCACTCACCC GACATCTGCC TCCGTGACTG TGACCACCCC AGGGCTCCTC CTGAGCCAGG CACTGCCGGC CCCCCCCGGC GCCCTCCCCC TGGCTCCCAG CAGTACCGCA CACAGCCAGA CCACCCCTCC GCCCTCATCA CGACCTCGGA CCACTGCCAG CGTCCCCAGG ACCACCGTGT GGCCCGTGCT GACGGTGCCC CCCACGGCAC CCTCCCCTGC ACCCAGCCTG GTGGCGTCCG CCTTTGGTGA ATCTGGCAGC ACTGATGGAA GCAGCGATGA GGAACTGAGC GGGGACCAGG AGGCCAGTGG GGGTGGCTCT GGGGGGCTCG AGCCCTTGGA GGGCAGCAGC GTGGCCACCC CTGGGCCACC TGTCGAGAGG GCTTCCTGCT ACAACTCCGC GTTGGGCTGC TGCTCTGATG GGAAGACGCC CTCGCTGGAC GCAGAGGGCT CCAACTGCCC CGCCACCAAG GTGTTCCAGG GCGTCCTGGA GCTGGAGGGC GTCGAGGGCC AGGAGCTGTT CTACACGCCC GAGATGGCTG ACCCCAAGTC AGAACTGTTC GGGGAGACAG CCAGGAGCAT TGAGAGCACC CTGGACGACC TCTTCCGGAA TTCAGACGTC AAGAAGGATT TTCGGAGTGT CCGCTTGCGG GACCTGGGGC CCGGCAAATC CGTCCGCGCC ATTGTGGATG TGCACTTTGA CCCCACCACA GCCTTCAGGG CACCCGACGT GGCCCGGGCC CTGCTCCGGC AGATCCAGGT GTCCAGGCGC CGGTCCTTGG GGGTGAGGCG GCCGCTGCAG GAGCACGTGC GATTTATGGA CTTTGACTGG TTTCCTGCGT TTATCACGGG GGCCACGTCA GGAGCCATTG CTGCGGGAGC CACGGCCAGA GCCACCACTG CATCGCGCCT GCCGTCCTCT GCTGTGACCC CTCGGGCCCC GCACCCCAGT CACACAAGCC AGCCCGTTGC CAAGACCACG GCAGCCCCCA CCACACGTCG GCCCCCCACC ACTGCCCCCA GCCGTGTGCC CGGACGTCGG CCCCCGGCCC CCCAGCAGCC TCCAAAGCCC TGTGACTCAC AGCCCTGCTT CCACGGGGGG ACCTGCCAGG ACTGGGCATT GGGCGGGGGC TTCACCTGCA GCTGCCCGGC AGGCAGGGGA GGCGCCGTCT GTGAGAAGGT GCTTGGCGCC CCTGTGCCGG CCTTCGAGGG CCGCTCCTTC CTGGCCTTCC CCACTCTCCG CGCCTACCAC ACGCTGCGCC TGGCACTGGA ATTCCGGGCG CTGGAGCCTC AGGGGCTGCT GCTGTACAAT GGCAACGCCC GGGGCAAGGA CTTCCTGGCA TTGGCGCTGC TAGATGGCCG CGTGCAGCTC AGGTTTGACA CAGGTTCGGG GCCGGCGGTG CTGACCAGTG CCGTGCCGGT AGAGCCGGGC CAGTGGCACC GCCTGGAGCT GTCCCGGCAC TGGCGCCGGG GCACCCTCTC GGTGGATGGT GAGACCCCTG TTCTGGGCGA GAGTCCCAGT GGCACCGACG GCCTCAACCT GGACACAGAC CTCTTTGTGG GCGGCGTACC CGAGGACCAG GCTGCCGTGG CGCTGGAGCG GACCTTCGTG GGCGCCGGCC TGAGGGGGTG CATCCGTTTG CTGGACGTCA ACAACCAGCG CCTGGAGCTT GGCATTGGGC CGGGGGCTGC CACCCGAGGC TCTGGCGTGG GCGAGTGCGG GGACCACCCC TGCCTGCCCA ACCCCTGCCA TGGCGGGGCC CCATGCCAGA ACCTGGAGGC TGGAAGGTTC CATTGCCAGT GCCCGCCCGG CCGCGTCGGA CCAACCTGTG CCGATGAGAA GAGCCCCTGC CAGCCCAACC CCTGCCATGG GGCGGCGCCC TGCCGTGTGC TGCCCGAGGG TGGTGCTCAG TGCGAGTGCC CCCTGGGGCG TGAGGGCACC TTCTGCCAGA CAGCCTCGGG GCAGGACGGC TCTGGGCCCT TCCTGGCTGA CTTCAACGGC TTCTCCCACC TGGAGCTGAG AGGCCTGCAC ACCTTTGCAC GGGACCTGGG GGAGAAGATG GCGCTGGAGG TCGTGTTCCT GGCACGAGGC CCCAGCGGCC TCCTGCTCTA CAACGGGCAG AAGACGGACG GCAAGGGGGA CTTCGTGTCG CTGGCACTGC GGGACCGCCG CCTGGAGTTC CGCTACGACC TGGGCAAGGG GGCAGCGGTC ATCAGGAGCA GGGAGCCAGT CACCCTGGGA GCCTGGACCA GGGTCTCACT GGAGCGAAAC GGCCGCAAGG GTGCCCTGCG TGTGGGCGAC GGCCCCCGTG TGTTGGGGGA GTCCCCGAAA TCCCGCAAGG TTCCGCACAC CGTCCTCAAC CTGAAGGAGC CGCTCTACGT AGGGGGCGCT CCCGACTTCA GCAAGCTGGC CCGTGCTGCT GCCGTGTCCT CTGGCTTCGA CGGTGCCATC CAGCTGGTCT CCCTCGGAGG CCGCCAGCTG CTGACCCCGG AGCACGTGCT GCGGCAGGTG GACGTCACGT CCTTTGCAGG TCACCCCTGC ACCCGGGCCT CAGGCCACCC CTGCCTCAAT GGGGCCTCCT GCGTCCCGAG GGAGGCTGCC TATGTGTGCC TGTGTCCCGG GGGATTCTCA GGACCGCACT GCGAGAAGGG GCTGGTGGAG AAGTCAGCGG GGGACGTGGA TACCTTGGCC TTTGACGGGC GGACCTTTGT CGAGTACCTC AACGCTGTGA CCGAGAGCGA ACTGGCCAAT GAGATCCCCG TCCCCGAAAC TCTGGATTCC GGGGCCCTTC ACAGCGAGAA GGCACTGCAG AGCAACCACT TTGAACTGAG CCTGCGCACT GAGGCCACGC AGGGGCTGGT GCTCTGGAGT GGCAAGGCCA CGGAGCGGGC AGACTATGTG GCACTGGCCA TTGTGGACGG GCACCTGCAA CTGAGCTACA ACCTGGGCTC CCAGCCCGTG GTGCTGCGTT CCACCGTGCC CGTCAACACC AACCGCTGGT TGCGGGTCGT GGCACATAGG GAGCAGAGGG AAGGTTCCCT GCAGGTGGGC AATGAGGCCC CTGTGACCGG CTCCTCCCCG CTGGGCGCCA CGCAGCTGGA CACTGATGGA GCCCTGTGGC TTGGGGGCCT GCCGGAGCTG CCCGTGGGCC CAGCACTGCC CAAGGCCTAC GGCACAGGCT TTGTGGGCTG CTTGCGGGAC GTGGTGGTGG GCCGGCACCC GCTGCACCTG CTGGAGGACG CCGTCACCAA GCCAGAGCTG CGGCCCTGCC CCACCCCAAT GATTCTCTGG GAAAAGAAAA GCGAAGATAT TACCGAGACA TTCAACCCCG ACAACAACAT GATCGCCGCC GACGCGAGCG AGGCATCGGA GGCGGGGAAG GCAGAAAACA CCCATTACAC CGGCAAGCAC CGCTTCATGT ACCGGATCCT GTTCGTCCTG ACCTGCCTGA TCACCTTCAT TGTGGTGGCT ATTGTCGGCG GAATTATCGC TTCGAACAGA AACGTCGGAA ATAGAATAGC TCAAGGCCAA GGAGAATTCG GAAGCGGAGC TACTAACTTC AGCCTGCTGA AGCAGGCTGG CGACGTGGAG GAGAACCCTG GACCTATGGT GAGCAAGGGC GAGGAGGATA ACATGGCCAT CATCAAGGAG TTCATGCGCT TCAAGGTGCA CATGGAGGGC TCCGTGAACG GCCACGAGTT CGAGATCGAG GGCGAGGGCG AGGGCCGCCC CTACGAGGGC ACCCAGACCG CCAAGCTGAA GGTGACCAAG GGTGGCCCCC TGCCCTTCGC CTGGGACATC CTGTCCCCTC AGTTCATGTA CGGCTCCAAG GCCTACGTGA AGCACCCCGC CGACATCCCC GACTACTTGA AGCTGTCCTT CCCCGAGGGC TTCAAGTGGG AGCGCGTGAT GAACTTCGAG GACGGCGGCG TGGTGACCGT GACCCAGGAC TCCTCCCTGC AGGACGGCGA GTTCATCTAC AAGGTGAAGC TGCGCGGCAC CAACTTCCCC TCCGACGGCC CCGTAATGCA GAAGAAGACC ATGGGCTGGG AGGCCTCCTC CGAGCGGATG TACCCCGAGG ACGGCGCCCT GAAGGGCGAG ATCAAGCAGA GGCTGAAGCT GAAGGACGGC GGCCACTACG ACGCTGAGGT CAAGACCACC TACAAGGCCA AGAAGCCCGT GCAGCTGCCC GGCGCCTACA ACGTCAACAT CAAGTTGGAC ATCACCTCCC ACAACGAGGA CTACACCATC GTGGAACAGT ACGAACGCGC CGAGGGCCGC CACTCCACCG GCGGCATGGA CGAGCTGTAC AAGTAACTCG AGTCTAGAGG GCCCCTTAAG GGTTCGATCC CTACCGGTTA GTAATGAGTT TGATATCTCG ACAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAGCT GACGTCCTTT CCATGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC CGCCTCCCCG CCTGGAAACG GGGGAGGCTA ACTGAAACAC GGAAGGAGAC AATACCGGAA GGAACCCGCG CTATGACGGC AATAAAAAGA CAGAATAAAA CGCACGGGTG TTGGGTCGTT TGTTCATAAA CGCGGGGTTC GGTCCCAGGG CTGGCACTCT GTCGATACCC CACCGAGACC CCATTGGGGC CAATACGCCC GCGTTTCTTC CTTTTCCCCA CCCCACCCCC CAAGTTCGGG TGAAGGCCCA GGGCTCGCAG CCAACGTCGG GGCGGCAGGC CCTGCCATAG CAGATCTGCG CAGCTGGGGC TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGCATCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGGGG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC AATGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCGACGGATC GGGAGATCTC CCGATCCCCT ATGGTCGACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATCTGCTCCC TGCTTGTGTG TTGGAGGTCG CTGAGTAGTG CGCGAGCAAA ATTTAAGCTA CAACAAGGCA AGGCTTGACC GACAATTGCA TGAAGAATCT GCTTAGG
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