pBaEVTR
$70.00
In stock
SKU
PL28192
·
100μg liquid plasmid(Instantly):$91
| name | pBaEVTR | another name | apBaEVTR |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Virus | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pMD2.G | 5′ sequencing primer | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AAAAAACATT TATTTTCATT GCAATGATGT ATTTAAATTA TTTCTGAATA TTTTACTAAA AAGGGAATGT GGGAGGTCAG TGCATTTAAA ACATAAAGAA ATGAAGAGCT AGTTCAAACC TTGGGAAAAT ACACTATATC TTAAACTCCA TGAAAGAAGG TGAGGCTGCA AACAGCTAAT GCACATTGGC AACAGCCCCT GATGCCTATG CCTTATTCAT CCCTCAGAAA AGGATTCAAG TAGAGGCTTG ATTTGGAGGT TAAAGTTTTG CTATGCTGTA TTTTACATTA CTTATTGTTT TAGCTGTCCT CATGAATGTC TTTTCACTAC CCATTTGCTT ATCCTGCATC TCTCAGCCTT GACTCCACTC AGTTCTCTTG CTTAGAGATA CCACCTTTCC CCTGAAGTGT TCCTTCCATG TTTTACGGCG AGATGGTTTC TCCTCGCCTG GCCACTCAGC CTTAGTTGTC TCTGTTGTCT TATAGAGGTC TACTTGAAGA AGGAAAAACA GGGGGCATGG TTTGACTGTC CTGTGAGCCC TTCTTCCCTG CCTCCCCCAC TCACAGTGAC CCGGAATCCC TCGACATGGC AGTCTAGCAC TAGTGCGGCC GCAGATCTGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTG GATCCCCTGA GGGGGCCCCC ATGGGCTAGA GGATCCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGAG CTTGGCCCAT TGCATACGTT GTATCCATAT CATAATATGT ACATTTATAT TGGCTCATGT CCAACATTAC CGCCATGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGACACC GGGACCGATC CAGCCTCCCC TCGAAGCTTA CATGTGGTAC CGAGCTCGGA TCCTGAGAAC TTCAGGGTGA GTCTATGGGA CCCTTGATGT TTTCTTTCCC CTTCTTTTCT ATGGTTAAGT TCATGTCATA GGAAGGGGAG AAGTAACAGG GTACACATAT TGACCAAATC AGGGTAATTT TGCATTTGTA ATTTTAAAAA ATGCTTTCTT CTTTTAATAT ACTTTTTTGT TTATCTTATT TCTAATACTT TCCCTAATCT CTTTCTTTCA GGGCAATAAT GATACAATGT ATCATGCCTC TTTGCACCAT TCTAAAGAAT AACAGTGATA ATTTCTGGGT TAAGGCAATA GCAATATTTC TGCATATAAA TATTTCTGCA TATAAATTGT AACTGATGTA AGAGGTTTCA TATTGCTAAT AGCAGCTACA ATCCAGCTAC CATTCTGCTT TTATTTTATG GTTGGGATAA GGCTGGATTA TTCTGAGTCC AAGCTAGGCC CTTTTGCTAA TCATGTTCAT ACCTCTTATC TTCCTCCCAC AGCTCCTGGG CAACGTGCTG GTCTGTGTGC TGGCCCATCA CTTTGGCAAA GCACGTGAGA TCTGAATTCG CCACCATGGG ATTCACAACA AAGATAATCT TCTTATACAA CCTAGTACTG GTCTACGCGG GGTTTGACGA CCCTCGCAAA GCCATAGAAC TAGTACAAAA GCGATATGGC CGACCATGCG ATTGCAGCGG AGGACAAGTG TCCGAGCCCC CGTCAGACAG GGTCAGTCAA GTGACTTGCT CAGGCAAGAC AGCTTACTTA ATGCCCGACC AAAGATGGAA ATGTAAGTCA ATTCCAAAAG ACACCTCCCC AAGCGGGCCA CTCCAAGAGT GCCCCTGTAA TTCTTACCAG TCCTCAGTAC ACAGTTCTTG TTATACCTCA TACCAACAAT GCAGATCAGG CAATAAGACA TATTATACGG CTACTCTGCT AAAAACACAA ACTGGGGGCA CCAGTGATGT ACAAGTATTA GGATCCACCA ACAAACTTAT ACAATCTCCC TGTAATGGCA TAAAAGGGCA GTCTATTTGC TGGAGCACTA CAGCTCCTAT CCACGTCTCT GATGGAGGAG GTCCATTAGA CACCACAAGA ATTAAAAGTG TTCAGAGAAA ACTGGAAGAA ATTCATAAAG CCCTATATCC TGAACTTCAG TATCACCCTT TGGCCATACC TAAGGTTAGA GATAACCTCA TGGTCGATGC CCAGACTTTA AACATTCTCA ATGCCACTTA CAACTTACTC CTAATGTCCA ACACGAGCCT AGTGGACGAC TGTTGGCTTT GTTTAAAATT AGGTCCCCCT ACTCCCCTCG CAATACCTAA CTTCCTATTA TCCTACGTGA CTCGCTCCTC GGATAATATC TCTTGTTTAA TAATTCCCCC CCTTCTAGTT CAACCGATGC AGTTTTCCAA TTCATCTTGC CTCTTTTCCC CCTCCTACAA CAGTACAGAA GAAATAGATC TAGGCCATGT TGCCTTCAGC AACTGTACCT CCATAACCAA TGTCACCGGT CCCATATGCG CTGTAAATGG TTCGGTCTTT CTCTGTGGCA ATAACATGGC ATACACTTAT CTACCCACGA ACTGGACGGG GCTTTGCGTC CTAGCAACTC TCCTCCCCGA CATTGACATC ATTCCCGGAG ATGAACCGGT CCCCATCCCT GCTATTGATC ATTTTATATA TAGACCTAAA CGGGCCATAC AGTTTATTCC TTTACTAGCA GGGCTAGGGA TCACCGCAGC CTTCACAACA GGAGCTACAG GCCTAGGTGT CTCTGTGACC CAATATACAA AATTATCTAA TCAGCTAATT TCTGATGTAC AAATCTTATC TAGCACCATA CAAGATCTGC AAGATCAAGT AGACTCATTA GCCGAAGTGG TTCTCCAGAA CAGAAGGGGG CTAGATCTAC TTACAGCAGA ACAAGGAGGA ATCTGTTTAG CCCTGCAAGA AAAATGCTGC TTTTATGTTA ACAAGTCAGG GATTGTGAGA GACAAAATAA AAACCTTACA AGAAGAACTA GAAAGACGTA GAAAAGATCT AGCTTCCAAC CCACTTTGGA CTGGGCTTCA AGGGCTCCTC CCTTACCTCC TGCCCTTTCT TGGCCCTCTA CTTACCCTCC TGCTCTTACT CACCATTGGG CCGTGCATTT TTAATCGATT AGTTCAATTT GTTAAAGACA GGATCTCAGT AGTCCAGGCT TTAGTCCTGA CTCAACAATA CCACCAGCTA AAACCACTAG AATACGAGCC ATAAGAATTC ACCCCACCAG TGCAGGCTGC CTATCAGAAA GTGGTGGCTG GTGTGGCTAA TGCCCTGGCC CACAAGTATC ACTAAGCTCG CTTTCTTGCT GTCCAATTTC TATTAAAGGT TCCTTTGTTC CCTAAGTCCA ACTACTAAAC TGGGGGATAT TATGAAGGGC CTTGAGCATC TGGATTCTGC CTAAT
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