PIK40286.1，putative ELKS/Rab6-interacting/CAST family member 1 isoform X2
100μg liquid plasmid(Instantly):$91
| name | pAOX1-α-factor-ENA(apostichopus-opt-yeast)-X2-6×His | another name | apAOX1-α-factor-ENA(apostichopus-opt-yeast)-X2-6×His |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Apostichopus japonicus | Prokaryotic resistance | Amp |
| Selective marker | HIS4 | Fluorescent labeling | |
| Promoter | AOX | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pAOX1-α-factor-ENA(apostichopus-opt-yeast)-X2 | 5′ sequencing primer | |
| 3′ sequencing primer | 3'AOX | Copy number | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGATCTAACA TCCAAAGACG AAAGGTTGAA TGAAACCTTT TTGCCATCCG ACATCCACAG GTCCATTCTC ACACATAAGT GCCAAACGCA ACAGGAGGGG ATACACTAGC AGCAGACCGT TGCAAACGCA GGACCTCCAC TCCTCTTCTC CTCAACACCC ACTTTTGCCA TCGAAAAACC AGCCCAGTTA TTGGGCTTGA TTGGAGCTCG CTCATTCCAA TTCCTTCTAT TAGGCTACTA ACACCATGAC TTTATTAGCC TGTCTATCCT GGCCCCCCTG GCGAGGTTCA TGTTTGTTTA TTTCCGAATG CAACAAGCTC CGCATTACAC CCGAACATCA CTCCAGATGA GGGCTTTCTG AGTGTGGGGT CAAATAGTTT CATGTTCCCC AAATGGCCCA AAACTGACAG TTTAAACGCT GTCTTGGAAC CTAATATGAC AAAAGCGTGA TCTCATCCAA GATGAACTAA GTTTGGTTCG TTGAAATGCT AACGGCCAGT TGGTCAAAAA GAAACTTCCA AAAGTCGCCA TACCGTTTGT CTTGTTTGGT ATTGATTGAC GAATGCTCAA AAATAATCTC ATTAATGCTT AGCGCAGTCT CTCTATCGCT TCTGAACCCC GGTGCACCTG TGCCGAAACG CAAATGGGGA AACACCCGCT TTTTGGATGA TTATGCATTG TCTCCACATT GTATGCTTCC AAGATTCTGG TGGGAATACT GCTGATAGCC TAACGTTCAT GATCAAAATT TAACTGTTCT AACCCCTACT TGACAGCAAT ATATAAACAG AAGGAAGCTG CCCTGTCTTA AACCTTTTTT TTTATCATCA TTATTAGCTT ACTTTCATAA TTGCGACTGG TTCCAATTGA CAAGCTTTTG ATTTTAACGA CTTTTAACGA CAACTTGAGA AGATCAAAAA ACAACTAATT ATTCGAAGGA TCCAAACGAT GAGATTTCCT TCAATTTTTA CTGCAGTTTT ATTCGCAGCA TCCTCCGCAT TAGCTGCTCC AGTCAACACT ACAACAGAAG ATGAAACGGC ACAAATTCCG GCTGAAGCTG TCATCGGTTA CTCAGATTTA GAAGGGGATT TCGATGTTGC TGTTTTGCCA TTTTCCAACA GCACAAATAA CGGGTTATTG TTTATAAATA CTACTATTGC CAGCATTGCT GCTAAAGAAG AAGGGGTATC TCTCGAGAAA AGAGAGGCTG AAGCTTACGT AGAATTCATG AGAACATTTG GTAGTATCGA ATTTAATATG AGAAGATGTT CTGAAGGTGA AGAAGGCGAA TCGAGAAAGT CAAATAGACC CAGTTTATTT TCCAATTACA CTTGTTGTAC CCAAGCTGCA TCAGATGGTT CCTTTTCTTG GACTTTGTCA AAGTCTAAGA GAATGGCTAA ACAATCTAAC TCTGGGCTTG ATATGTATTC ATCTCAAAGT CCCAGACACT CTCCAACACC ACCATCCCAA TCACAACAAG TTAACAATAA TTCTTTTGAC CAAGATGCAA GCTCTGGTTC ATCTTCAGGT TCTGGTACTG CTAGTAGTAA TTCCACCCCA AGATCTCCAA GCAGATCAAA ATCCCCAAGA CCACATAGAG TTGAAGGTTC CAGTAGATTT CCTGAAGCAA AAATGGAAAC TTTTCAGTCC TTCAATGCTG CATATAACGC TGGCCCAGTT TACATCTCTG AAGGTTTGGG TTCTCCAGTT CCAGGTGGTT CTACTTCCCC AAATTCATCT GGTAATCATG CTAGAAGTAA TTCCCCAGTC CATTCCAGAT CTTCTGGTTC TAGTCAAGGT AGTGGTTCTA ATATCACTTT AGGTCACGGT ACTCATCCAG GTGCTCATCC ACCAGGTCAT CCATTGCATC ATAGAGCTCC AGATTTTGAC TCTATGGACG TTTCTTCACA ATTGAGAGAA TTGCAAAGAG AAAACACAAT GTATAGACAA GAACTAGATG CTAGAGACGC TAAATTGTCC TCCTCAATGA ATTCTATTAA GACATTCTGG TCACCAGAAT TGAAAAAAGA AAGGGCTTTA AGAAAGGAAG AGGCTGGTAA GATTCAGGCT TTGAAAGAAC AATGTAAAAA AGCCGTTGAA GAAAATCAGG TCTCATTGTT GTTTGATTTG TCACCATGCG GCCGCCACCA TCACCATCAC CATTAACCGG TCTTGCTAGA TTCTAATCAA GAGGATGTCA GAATGCCATT TGCCTGAGAG ATGCAGGCTT CATTTTTGAT ACTTTTTTAT TTGTAACCTA TATAGTATAG GATTTTTTTT GTCATTTTGT TTCTTCTCGT ACGAGCTTGC TCCTGATCAG CCTATCTCGC AGCTGATGAA TATCTTGTGG TAGGGGTTTG GGAAAATCAT TCGAGTTTGA TGTTTTTCTT GGTATTTCCC ACTCCTCTTC AGAGTACAGA AGATTAAGTG AGACGTTCGT TTGTGCAAGC TTATCGATAA GCTTTAATGC GGTAGTTTAT CACAGTTAAA TTGCTAACGC AGTCAGGCAC CGTGTATGAA ATCTAACAAT GCGCTCATCG TCATCCTCGG CACCGTCACC CTGGATGCTG TAGGCATAGG CTTGGTTATG CCGGTACTGC CGGGCCTCTT GCGGGATATC GTCCATTCCG ACAGCATCGC CAGTCACTAT GGCGTGCTGC TAGCGCTATA TGCGTTGATG CAATTTCTAT GCGCACCCGT TCTCGGAGCA CTGTCCGACC GCTTTGGCCG CCGCCCAGTC CTGCTCGCTT CGCTACTTGG AGCCACTATC GACTACGCGA TCATGGCGAC CACACCCGTC CTGTGGATCT ATCGAATCTA AATGTAAGTT AAAATCTCTA AATAATTAAA TAAGTCCCAG TTTCTCCATA CGAACCTTAA CAGCATTGCG GTGAGCATCT AGACCTTCAA CAGCAGCCAG ATCCATCACT GCTTGGCCAA TATGTTTCAG TCCCTCAGGA GTTACGTCTT GTGAAGTGAT GAACTTCTGG AAGGTTGCAG TGTTAACTCC GCTGTATTGA CGGGCATATC CGTACGTTGG CAAAGTGTGG TTGGTACCGG AGGAGTAATC TCCACAACTC TCTGGAGAGT AGGCACCAAC AAACACAGAT CCAGCGTGTT GTACTTGATC AACATAAGAA GAAGCATTCT CGATTTGCAG GATCAAGTGT TCAGGAGCGT ACTGATTGGA CATTTCCAAA GCCTGCTCGT AGGTTGCAAC CGATAGGGTT GTAGAGTGTG CAATACACTT GCGTACAATT TCAACCCTTG GCAACTGCAC AGCTTGGTTG TGAACAGCAT CTTCAATTCT GGCAAGCTCC TTGTCTGTCA TATCGACAGC CAACAGAATC ACCTGGGAAT CAATACCATG TTCAGCTTGA GACAGAAGGT CTGAGGCAAC GAAATCTGGA TCAGCGTATT TATCAGCAAT AACTAGAACT TCAGAAGGCC CAGCAGGCAT GTCAATACTA CACAGGGCTG ATGTGTCATT TTGAACCATC ATCTTGGCAG CAGTAACGAA CTGGTTTCCT GGACCAAATA TTTTGTCACA CTTAGGAACA GTTTCTGTTC CGTAAGCCAT AGCAGCTACT GCCTGGGCGC CTCCTGCTAG CACGATACAC TTAGCACCAA CCTTGTGGGC AACGTAGATG ACTTCTGGGG TAAGGGTACC ATCCTTCTTA GGTGGAGATG CAAAAACAAT TTCTTTGCAA CCAGCAACTT TGGCAGGAAC ACCCAGCATC AGGGAAGTGG AAGGCAGAAT TGCGGTTCCA CCAGGAATAT AGAGGCCAAC TTTCTCAATA GGTCTTGCAA AACGAGAGCA GACTACACCA GGGCAAGTCT CAACTTGCAA CGTCTCCGTT AGTTGAGCTT CATGGAATTT CCTGACGTTA TCTATAGAGA GATCAATGGC TCTCTTAACG TTATCTGGCA ATTGCATAAG TTCCTCTGGG AAAGGAGCTT CTAACACAGG TGTCTTCAAA GCGACTCCAT CAAACTTGGC AGTTAGTTCT AAAAGGGCTT TGTCACCATT TTGACGAACA TTGTCGACAA TTGGTTTGAC TAATTCCATA ATCTGTTCCG TTTTCTGGAT AGGACGACGA AGGGCATCTT CAATTTCTTG TGAGGAGGCC TTAGAAACGT CAATTTTGCA CAATTCAATA CGACCTTCAG AAGGGACTTC TTTAGGTTTG GATTCTTCTT TAGGTTGTTC CTTGGTGTAT CCTGGCTTGG CATCTCCTTT CCTTCTAGTG ACCTTTAGGG ACTTCATATC CAGGTTTCTC TCCACCTCGT CCAACGTCAC ACCGTACTTG GCACATCTAA CTAATGCAAA ATAAAATAAG TCAGCACATT CCCAGGCTAT ATCTTCCTTG GATTTAGCTT CTGCAAGTTC ATCAGCTTCC TCCCTAATTT TAGCGTTCAA CAAAACTTCG TCGTCAAATA ACCGTTTGGT ATAAGAACCT TCTGGAGCAT TGCTCTTACG ATCCCACAAG GTGGCTTCCA TGGCTCTAAG ACCCTTTGAT TGGCCAAAAC AGGAAGTGCG TTCCAAGTGA CAGAAACCAA CACCTGTTTG TTCAACCACA AATTTCAAGC AGTCTCCATC ACAATCCAAT TCGATACCCA GCAACTTTTG AGTTGCTCCA GATGTAGCAC CTTTATACCA CAAACCGTGA CGACGAGATT GGTAGACTCC AGTTTGTGTC CTTATAGCCT CCGGAATAGA CTTTTTGGAC GAGTACACCA GGCCCAACGA GTAATTAGAA GAGTCAGCCA CCAAAGTAGT GAATAGACCA TCGGGGCGGT CAGTAGTCAA AGACGCCAAC AAAATTTCAC TGACAGGGAA CTTTTTGACA TCTTCAGAAA GTTCGTATTC AGTAGTCAAT TGCCGAGCAT CAATAATGGG GATTATACCA GAAGCAACAG TGGAAGTCAC ATCTACCAAC TTTGCGGTCT CAGAAAAAGC ATAAACAGTT CTACTACCGC CATTAGTGAA ACTTTTCAAA TCGCCCAGTG GAGAAGAAAA AGGCACAGCG ATACTAGCAT TAGCGGGCAA GGATGCAACT TTATCAACCA GGGTCCTATA GATAACCCTA GCGCCTGGGA TCATCCTTTG GACAACTCTT TCTGCCAAAT CTAGGTCCAA AATCACTTCA TTGATACCAT TATTGTACAA CTTGAGCAAG TTGTCGATCA GCTCCTCAAA TTGGTCCTCT GTAACGGATG ACTCAACTTG CACATTAACT TGAAGCTCAG TCGATTGAGT GAACTTGATC AGGTTGTGCA GCTGGTCAGC AGCATAGGGA AACACGGCTT TTCCTACCAA ACTCAAGGAA TTATCAAACT CTGCAACACT TGCGTATGCA GGTAGCAAGG GAAATGTCAT ACTTGAAGTC GGACAGTGAG TGTAGTCTTG AGAAATTCTG AAGCCGTATT TTTATTATCA GTGAGTCAGT CATCAGGAGA TCCTCTACGC CGGACGCATC GTGGCCGACC TGCAGGGGGG GGGGGGGCGC TGAGGTCTGC CTCGTGAAGA AGGTGTTGCT GACTCATACC AGGCCTGAAT CGCCCCATCA TCCAGCCAGA AAGTGAGGGA GCCACGGTTG ATGAGAGCTT TGTTGTAGGT GGACCAGTTG GTGATTTTGA ACTTTTGCTT TGCCACGGAA CGGTCTGCGT TGTCGGGAAG ATGCGTGATC TGATCCTTCA ACTCAGCAAA AGTTCGATTT ATTCAACAAA GCCGCCGTCC CGTCAAGTCA GCGTAATGCT CTGCCAGTGT TACAACCAAT TAACCAATTC TGATTAGAAA AACTCATCGA GCATCAAATG AAACTGCAAT TTATTCATAT CAGGATTATC AATACCATAT TTTTGAAAAA GCCGTTTCTG TAATGAAGGA GAAAACTCAC CGAGGCAGTT CCATAGGATG GCAAGATCCT GGTATCGGTC TGCGATTCCG ACTCGTCCAA CATCAATACA ACCTATTAAT TTCCCCTCGT CAAAAATAAG GTTATCAAGT GAGAAATCAC CATGAGTGAC GACTGAATCC GGTGAGAATG GCAAAAGCTT ATGCATTTCT TTCCAGACTT GTTCAACAGG CCAGCCATTA CGCTCGTCAT CAAAATCACT CGCATCAACC AAACCGTTAT TCATTCGTGA TTGCGCCTGA GCGAGACGAA ATACGCGATC GCTGTTAAAA GGACAATTAC AAACAGGAAT CGAATGCAAC CGGCGCAGGA ACACTGCCAG CGCATCAACA ATATTTTCAC CTGAATCAGG ATATTCTTCT AATACCTGGA ATGCTGTTTT CCCGGGGATC GCAGTGGTGA GTAACCATGC ATCATCAGGA GTACGGATAA AATGCTTGAT GGTCGGAAGA GGCATAAATT CCGTCAGCCA GTTTAGTCTG ACCATCTCAT CTGTAACATC ATTGGCAACG CTACCTTTGC CATGTTTCAG AAACAACTCT GGCGCATCGG GCTTCCCATA CAATCGATAG ATTGTCGCAC CTGATTGCCC GACATTATCG CGAGCCCATT TATACCCATA TAAATCAGCA TCCATGTTGG AATTTAATCG CGGCCTCGAG CAAGACGTTT CCCGTTGAAT ATGGCTCATA ACACCCCTTG TATTACTGTT TATGTAAGCA GACAGTTTTA TTGTTCATGA TGATATATTT TTATCTTGTG CAATGTAACA TCAGAGATTT TGAGACACAA CGTGGCTTTC CCCCCCCCCC CTGCAGGTCG GCATCACCGG CGCCACAGGT GCGGTTGCTG GCGCCTATAT CGCCGACATC ACCGATGGGG AAGATCGGGC TCGCCACTTC GGGCTCATGA GCGCTTGTTT CGGCGTGGGT ATGGTGGCAG GCCCCGTGGC CGGGGGACTG TTGGGCGCCA TCTCCTTGCA TGCACCATTC CTTGCGGCGG CGGTGCTCAA CGGCCTCAAC CTACTACTGG GCTGCTTCCT AATGCAGGAG TCGCATAAGG GAGAGCGTCG AGTATCTATG ATTGGAAGTA TGGGAATGGT GATACCCGCA TTCTTCAGTG TCTTGAGGTC TCCTATCAGA TTATGCCCAA CTAAAGCAAC CGGAGGAGGA GATTTCATGG TAAATTTCTC TGACTTTTGG TCATCAGTAG ACTCGAACTG TGAGACTATC TCGGTTATGA CAGCAGAAAT GTCCTTCTTG GAGACAGTAA ATGAAGTCCC ACCAATAAAG AAATCCTTGT TATCAGGAAC AAACTTCTTG TTTCGAACTT TTTCGGTGCC TTGAACTATA AAATGTAGAG TGGATATGTC GGGTAGGAAT GGAGCGGGCA AATGCTTACC TTCTGGACCT TCAAGAGGTA TGTAGGGTTT GTAGATACTG ATGCCAACTT CAGTGACAAC GTTGCTATTT CGTTCAAACC ATTCCGAATC CAGAGAAATC AAAGTTGTTT GTCTACTATT GATCCAAGCC AGTGCGGTCT TGAAACTGAC AATAGTGTGC TCGTGTTTTG AGGTCATCTT TGTATGAATA AATCTAGTCT TTGATCTAAA TAATCTTGAC GAGCCAAGGC GATAAATACC CAAATCTAAA ACTCTTTTAA AACGTTAAAA GGACAAGTAT GTCTGCCTGT ATTAAACCCC AAATCAGCTC GTAGTCTGAT CCTCATCAAC TTGAGGGGCA CTATCTTGTT TTAGAGAAAT TTGCGGAGAT GCGATATCGA GAAAAAGGTA CGCTGATTTT AAACGTGAAA TTTATCTCAA GATCTCTGCC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCGCA GCCATGACCC AGTCACGTAG CGATAGCGGA GTGTATACTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCAATG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTGCA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAACA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTTCAAGAA TTAATTCTCA TGTTTGACAG CTTATCATCG ATAAGCTGAC TCATGTTGGT ATTGTGAAAT AGACGCAGAT CGGGAACACT GAAAAATAAC AGTTATTATT CG
