pCMV-AARS2(human)-Myc-Neo
$84.00
In stock
SKU
PL25057
NM_020745.4;AARSL; COXPD8; LKENP; MT-ALARS; MTALARS
100μg liquid plasmid(Instantly):$105
| name | pCMV-AARS2(human)-Myc-Neo | another name | apCMV-AARS2(human)-Myc-Neo |
| Insert Size (bp) | 2955bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pcDNA3.1-Myc-C | 5′ sequencing primer | CMV-F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGT TAAGCTTGGT ACCGAGCTCG GATCCACTAG TCCAGTGTGG TGGAATTCGC CACCATGGCA GCGTCAGTGG CAGCTGCAGC CCGGAGGCTG CGGCGGGCCA TTCGAAGGTC GCCCGCATGG CGGGGCCTCA GCCATCGGCC GCTCTCATCG GAGCCCCCTG CAGCCAAGGC CTCGGCCGTG AGGGCCGCCT TTCTGAACTT CTTTCGGGAC CGCCATGGCC ACCGGCTGGT GCCCTCCGCT TCCGTGCGGC CCCGCGGCGA CCCCAGTTTG CTTTTTGTCA ATGCGGGCAT GAACCAGTTC AAGCCAATCT TTCTGGGCAC CGTGGATCCA CGAAGCGAGA TGGCAGGCTT CCGACGTGTG GCCAACAGCC AGAAATGTGT GAGAGCTGGA GGACACCATA ACGACCTGGA AGATGTGGGT CGAGACCTTT CCCATCATAC CTTCTTTGAA ATGCTTGGCA ATTGGGCCTT TGGGGGTGAA TATTTTAAGG AGGAGGCTTG TAACATGGCC TGGGAACTGC TGACTCAGGT CTATGGGATC CCTGAGGAAA GGCTCTGGAT CTCCTACTTT GATGGTGACC CCAAGGCAGG GCTGGACCCA GACCTGGAGA CCAGGGACAT CTGGCTGAGC TTAGGGGTGC CTGCTAGCCG TGTGCTTTCC TTTGGACCAC AAGAGAACTT CTGGGAGATG GGGGATACTG GCCCTTGTGG GCCCTGTACT GAGATCCACT ACGACCTTGC TGGTGGGGTG GGAGCCCCCC AGCTGGTAGA GCTTTGGAAC CTGGTCTTCA TGCAACACAA CAGAGAGGCA GATGGAAGCC TGCAGCCCCT GCCCCAGCGG CATGTGGACA CAGGAATGGG CCTGGAAAGG CTGGTGGCTG TGCTGCAAGG CAAACACTCC ACCTATGACA CTGACCTCTT TTCCCCGCTG CTCAACGCCA TACAGCAGGG CTGCAGGGCA CCCCCTTACT TGGGCCGAGT AGGGGTGGCA GACGAGGGGC GCACAGACAC AGCGTACCGC GTGGTGGCTG ACCACATCCG CACACTCAGT GTCTGCATCT CTGATGGCAT CTTCCCTGGG ATGTCAGGTC CCCCGCTGGT TCTTCGTCGG ATCCTGCGTC GAGCTGTGCG TTTCTCCATG GAGATCTTAA AGGCACCACC TGGCTTCCTA GGCAGCCTGG TACCTGTAGT GGTGGAGACA CTGGGAGATG CTTATCCAGA ACTGCAAAGG AACTCAGCCC AGATCGCCAA CCTGGTGTCA GAGGACGAGG CAGCCTTCCT GGCCTCCCTG GAGCGGGGTA GGCGGATCAT TGATCGGACT CTGAGGACCC TGGGGCCTTC AGATATGTTC CCTGCTGAAG TGGCCTGGTC CTTGTCACTG TGTGGAGACC TGGGACTCCC CTTGGACATG GTAGAGCTGA TGCTGGAGGA GAAAGGGGTC CAGCTAGACT CCGCTGGACT GGAGCGGTTG GCCCAAGAGG AGGCCCAGCA CCGGGCACGG CAGGCTGAGC CAGTTCAGAA GCAGGGATTG TGGCTTGATG TCCATGCGCT TGGGGAGCTG CAGCGCCAAG GAGTGCCCCC AACTGACGAC AGCCCCAAGT ACAACTACTC CCTGCGACCC AGCGGAAGTT ATGAGTTCGG CACCTGTGAG GCCCAGGTGT TGCAACTGTA TACAGAGGAC GGGACAGCAG TGGCCTCCGT GGGGAAAGGC CAGCGCTGTG GCCTCCTCTT GGACAGGACC AACTTCTACG CAGAACAGGG GGGCCAGGCT TCAGACCGTG GCTACCTGGT GCGGGCAGGG CAAGAGGACG TGCTGTTCCC AGTAGCCCGG GCCCAGGTCT GTGGAGGTTT CATCCTGCAT GAGGCAGTAG CCCCTGAGTG CCTGCGGTTA GGGGACCAGG TGCAGCTGCA TGTGGATGAG GCCTGGCGTC TAGGCTGCAT GGCGAAGCAT ACGGCCACCC ACCTGCTGAA CTGGGCACTG AGGCAGACCC TGGGCCCTGG CACAGAGCAG CAGGGCTCCC ATCTCAATCC TGAGCAGCTG CGCTTGGATG TGACCACCCA GACCCCATTG ACCCCAGAGC AGCTCCGGGC AGTGGAGAAC ACTGTGCAGG AGGCCGTGGG GCAGGATGAG GCTGTGTACA TGGAGGAGGT GCCCCTGGCG CTCACTGCCC AGGTCCCTGG CCTGCGCTCT CTGGATGAGG TTTACCCAGA CCCTGTGCGG GTGGTATCAG TGGGGGTGCC CGTGGCCCAT GCATTGGACC CAGCCTCCCA AGCCGCACTG CAGACCTCTG TGGAGCTATG CTGTGGGACG CACCTGTTAC GTACTGGGGC TGTAGGGGAC CTGGTTATCA TCGGGGACCG CCAGCTTTCC AAGGGCACTA CCCGCCTGCT GGCCGTCACT GGGGAGCAGG CCCAGCAGGC CCGAGAGCTA GGCCAGAGCC TGGCCCAGGA AGTGAAAGCG GCCACTGAGC GGCTGAGTCT GGGGAGCCGG GATGTGGCGG AGGCACTGAG GCTGTCCAAG GACATAGGAC GACTCATTGA AGCTGTGGAA ACTGCTGTGA TGCCCCAGTG GCAGCGGCGG GAGCTGCTGG CCACAGTGAA GATGCTGCAG CGGCGTGCCA ACACTGCCAT CCGTAAGCTG CAAATGGGAC AGGCTGCAAA GAAAACTCAG GAGCTGCTGG AGCGGCACTC GAAGGGGCCT CTGATTGTGG ACACAGTCTC TGCTGAGTCT CTCTCAGTGC TGGTGAAGGT GGTACGGCAG CTGTGTGAGC AGGCCCCCAG CACGTCTGTG CTCCTACTCA GCCCCCAGCC CATGGGGAAG GTGCTGTGTG CCTGTCAGGT GGCCCAGGGT GCCATGCCCA CCTTCACAGC AGAGGCCTGG GCACTGGCAG TGTGCAGCCA CATGGGGGGC AAGGCGTGGG GCTCACGAGT GGTGGCCCAA GGCACCGGAA GCACTACTGA CCTGGAAGCT GCCCTCAGTA TAGCCCAAAC CTATGCCCTC AGCCAGCTCC TCGAGTCTAG AGGGCCCTTC GAACAAAAAC TCATCTCAGA AGAGGATCTG TGAGTTTAAA CCCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA TGGCTTCTGA GGCGGAAAGA ACCAGCTGGG GCTCTAGGGG GTATCCCCAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTAAT TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTC
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