pGADT7-Ppp1cc(mouse)
$70.00
In stock
SKU
PL24873
NM_001424537.1;dis2m1; PP-1G; PP1
100μg liquid plasmid(Instantly):$91
| name | pGADT7-Ppp1cc(mouse) | another name | apGADT7-Ppp1cc(mouse) |
| Insert Size (bp) | 1011bp | Plasmid host | Yeast bacterium |
| Plasmid uses | hybridization | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | LEU2 | Fluorescent labeling | |
| Promoter | ADH1 | Replicon | 2U |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pGADT7 | 5′ sequencing primer | T7 |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGCATGCCTG CAGGTCGAGA TCCGGGATCG AAGAAATGAT GGTAAATGAA ATAGGAAATC AAGGAGCATG AAGGCAAAAG ACAAATATAA GGGTCGAACG AAAAATAAAG TGAAAAGTGT TGATATGATG TATTTGGCTT TGCGGCGCCG AAAAAACGAG TTTACGCAAT TGCACAATCA TGCTGACTCT GTGGCGGACC CGCGCTCTTG CCGGCCCGGC GATAACGCTG GGCGTGAGGC TGTGCCCGGC GGAGTTTTTT GCGCCTGCAT TTTCCAAGGT TTACCCTGCG CTAAGGGGCG AGATTGGAGA AGCAATAAGA ATGCCGGTTG GGGTTGCGAT GATGACGACC ACGACAACTG GTGTCATTAT TTAAGTTGCC GAAAGAACCT GAGTGCATTT GCAACATGAG TATACTAGAA GAATGAGCCA AGACTTGCGA GACGCGAGTT TGCCGGTGGT GCGAACAATA GAGCGACCAT GACCTTGAAG GTGAGACGCG CATAACCGCT AGAGTACTTT GAAGAGGAAA CAGCAATAGG GTTGCTACCA GTATAAATAG ACAGGTACAT ACAACACTGG AAATGGTTGT CTGTTTGAGT ACGCTTTCAA TTCATTTGGG TGTGCACTTT ATTATGTTAC AATATGGAAG GGAACTTTAC ACTTCTCCTA TGCACATATA TTAATTAAAG TCCAATGCTA GTAGAGAAGG GGGGTAACAC CCCTCCGCGC TCTTTTCCGA TTTTTTTCTA AACCGTGGAA TATTTCGGAT ATCCTTTTGT TGTTTCCGGG TGTACAATAT GGACTTCCTC TTTTCTGGCA ACCAAACCCA TACATCGGGA TTCCTATAAT ACCTTCGTTG GTCTCCCTAA CATGTAGGTG GCGGAGGGGA GATATACAAT AGAACAGATA CCAGACAAGA CATAATGGGC TAAACAAGAC TACACCAATT ACACTGCCTC ATTGATGGTG GTACATAACG AACTAATACT GTAGCCCTAG ACTTGATAGC CATCATCATA TCGAAGTTTC ACTACCCTTT TTCCATTTGC CATCTATTGA AGTAATAATA GGCGCATGCA ACTTCTTTTC TTTTTTTTTC TTTTCTCTCT CCCCCGTTGT TGTCTCACCA TATCCGCAAT GACAAAAAAA ATGATGGAAG ACACTAAAGG AAAAAATTAA CGACAAAGAC AGCACCAACA GATGTCGTTG TTCCAGAGCT GATGAGGGGT ATCTCGAAGC ACACGAAACT TTTTCCTTCC TTCATTCACG CACACTACTC TCTAATGAGC AACGGTATAC GGCCTTCCTT CCAGTTACTT GAATTTGAAA TAAAAAAAAG TTTGCTGTCT TGCTATCAAG TATAAATAGA CCTGCAATTA TTAATCTTTT GTTTCCTCGT CATTGTTCTC GTTCCCTTTC TTCCTTGTTT CTTTTTCTGC ACAATATTTC AAGCTATACC AAGCATACAA TCAACTCCAA GCTTTGCAAA GATGGATAAA GCGGAATTAA TTCCCGAGCC TCCAAAAAAG AAGAGAAAGG TCGAATTGGG TACCGCCGCC AATTTTAATC AAAGTGGGAA TATTGCTGAT AGCTCATTGT CCTTCACTTT CACTAACAGT AGCAACGGTC CGAACCTCAT AACAACTCAA ACAAATTCTC AAGCGCTTTC ACAACCAATT GCCTCCTCTA ACGTTCATGA TAACTTCATG AATAATGAAA TCACGGCTAG TAAAATTGAT GATGGTAATA ATTCAAAACC ACTGTCACCT GGTTGGACGG ACCAAACTGC GTATAACGCG TTTGGAATCA CTACAGGGAT GTTTAATACC ACTACAATGG ATGATGTATA TAACTATCTA TTCGATGATG AAGATACCCC ACCAAACCCA AAAAAAGAGA TCTTTAATAC GACTCACTAT AGGGCGAGCG CCGCCATGGA GTACCCATAC GACGTACCAG ATTACGCTCA TATGGCCATG GAGGCCAGTG AATTCATGGC GGATATCGAC AAACTCAACA TCGACAGCAT CATCCAACGG CTGCTGGAAG TGAGAGGGTC CAAGCCAGGC AAGAATGTCC AGCTCCAGGA GAACGAGATC CGAGGACTCT GCCTGAAGTC TCGGGAGATC TTCCTCAGTC AGCCTATCCT TTTAGAACTT GAAGCACCAC TCAAGATATG TGGTGACATC CACGGGCAGT ACTATGATTT GCTCCGTCTG TTTGAATACG GTGGCTTTCC TCCAGAGAGC AACTATTTGT TTCTCGGGGA CTATGTGGAC AGGGGCAAGC AGTCCCTGGA GACAATCTGC CTCTTGCTGG CCTACAAAAT CAAGTATCCG GAGAACTTCT TTCTTCTCAG AGGGAACCAC GAGTGCGCCA GCATCAATAG GATCTACGGA TTTTATGATG AGTGTAAAAG AAGATACAAC ATTAAGCTGT GGAAAACGTT CACAGACTGT TTTAACTGCT TGCCGATAGC AGCCATCGTG GACGAGAAGA TATTCTGCTG TCATGGAGGT TTATCACCAG ATCTTCAATC TATGGAGCAG ATTCGGCGAA TTATGAGACC AACTGATGTA CCAGATCAAG GTCTTCTTTG TGATCTTTTG TGGTCTGACC CCGATAAAGA TGTCTTAGGC TGGGGTGAAA ATGACAGAGG AGTGTCCTTC ACATTTGGTG CAGAAGTGGT TGCAAAATTT CTCCATAAGC ATGATTTGGA TCTTATATGT AGAGCCCATC AGGTGGTTGA AGATGGCTAT GAGTTTTTTG CAAAGAGGCA GTTAGTCACT CTGTTTTCTG CACCCAACTA CTGTGGCGAG TTTGACAATG CAGGCGCCAT GATGAGTGTG GATGAGACCC TCATGTGTTC CTTCCAGATT TTAAAGCCTG CAGAGAAAAA GAAGCCCAAC GCCACGAGAC CTGTCACACC ACCACGGGTT GGATCAGGCC TGAACCCGTC CATTCAGAAA GCTTCAAATT ATAGAAACAA CACTGTCCTA TACGAGTGAG GATCCATCGA GCTCGAGCTG CAGATGAATC GTAGATACTG AAAAACCCCG CAAGTTCACT TCAACTGTGC ATCGTGCACC ATCTCAATTT CTTTCATTTA TACATCGTTT TGCCTTCTTT TATGTAACTA TACTCCTCTA AGTTTCAATC TTGGCCATGT AACCTCTGAT CTATAGAATT TTTTAAATGA CTAGAATTAA TGCCCATCTT TTTTTTGGAC CTAAATTCTT CATGAAAATA TATTACGAGG GCTTATTCAG AAGCTTTGGA CTTCTTCGCC AGAGGTTTGG TCAAGTCTCC AATCAAGGTT GTCGGCTTGT CTACCTTGCC AGAAATTTAC GAAAAGATGG AAAAGGGTCA AATCGTTGGT AGATACGTTG TTGACACTTC TAAATAAGCG AATTTCTTAT GATTTATGAT TTTTATTATT AAATAAGTTA TAAAAAAAAT AAGTGTATAC AAATTTTAAA GTGACTCTTA GGTTTTAAAA CGAAAATTCT TATTCTTGAG TAACTCTTTC CTGTAGGTCA GGTTGCTTTC TCAGGTATAG CATGAGGTCG CTCTTATTGA CCACACCTCT ACCGGCCGGT CGAAATTCCC CTACCCTATG AACATATTCC ATTTTGTAAT TTCGTGTCGT TTCTATTATG AATTTCATTT ATAAAGTTTA TGTACAAATA TCATAAAAAA AGAGAATCTT TTTAAGCAAG GATTTTCTTA ACTTCTTCGG CGACAGCATC ACCGACTTCG GTGGTACTGT TGGAACCACC TAAATCACCA GTTCTGATAC CTGCATCCAA AACCTTTTTA ACTGCATCTT CAATGGCCTT ACCTTCTTCA GGCAAGTTCA ATGACAATTT CAACATCATT GCAGCAGACA AGATAGTGGC GATAGGGTTG ACCTTATTCT TTGGCAAATC TGGAGCAGAA CCGTGGCATG GTTCGTACAA ACCAAATGCG GTGTTCTTGT CTGGCAAAGA GGCCAAGGAC GCAGATGGCA ACAAACCCAA GGAACCTGGG ATAACGGAGG CTTCATCGGA GATGATATCA CCAAACATGT TGCTGGTGAT TATAATACCA TTTAGGTGGG TTGGGTTCTT AACTAGGATC ATGGCGGCAG AATCAATCAA TTGATGTTGA ACCTTCAATG TAGGAAATTC GTTCTTGATG GTTTCCTCCA CAGTTTTTCT CCATAATCTT GAAGAGGCCA AAACATTAGC TTTATCCAAG GACCAAATAG GCAATGGTGG CTCATGTTGT AGGGCCATGA AAGCGGCCAT TCTTGTGATT CTTTGCACTT CTGGAACGGT GTATTGTTCA CTATCCCAAG CGACACCATC ACCATCGTCT TCCTTTCTCT TACCAAAGTA AATACCTCCC ACTAATTCTC TGACAACAAC GAAGTCAGTA CCTTTAGCAA ATTGTGGCTT GATTGGAGAT AAGTCTAAAA GAGAGTCGGA TGCAAAGTTA CATGGTCTTA AGTTGGCGTA CAATTGAAGT TCTTTACGGA TTTTTAGTAA ACCTTGTTCA GGTCTAACAC TACCTGTACC CCATTTAGGA CCACCCACAG CACCTAACAA AACGGCATCA ACCTTCTTGG AGGCTTCCAG CGCCTCATCT GGAAGTGGGA CACCTGTAGC GTCGATAGCA GCACCACCAA TTAAATGATT TTCGAAATCG AACTTGACAT TGGAACGAAC ATCAGAAATA GCTTTAAGAA CCTTAATGGC TTCGGCTGTG ATTTCTTGAC CAACGTGGTC ACCTGGCAAA ACGACGATCT TCTTAGGGGC AGACATTAGA ATGGTATATC CTTGAAATAT ATATATATAT TGCTGAAATG TAAAAGGTAA GAAAAGTTAG AAAGTAAGAC GATTGCTAAC CACCTATTGG AAAAAACAAT AGGTCCTTAA ATAATATTGT CAACTTCAAG TATTGTGATG CAAGCATTTA GTCATGAACG CTTCTCTATT CTATATGAAA AGCCGGTTCC GGCGCTCTCA CCTTTCCTTT TTCTCCCAAT TTTTCAGTTG AAAAAGGTAT ATGCGTCAGG CGACCTCTGA AATTAACAAA AAATTTCCAG TCATCGAATT TGATTCTGTG CGATAGCGCC CCTGTGTGTT CTCGTTATGT TGAGGAAAAA AATAATGGTT GCTAAGAGAT TCGAACTCTT GCATCTTACG ATACCTGAGT ATTCCCACAG TTGGGGATCT CGACTCTAGC TAGAGGATCA ATTCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGATAACTTC GTATAATGTA TGCTATACGA AGTTATTAGG TCTGAAGAGG AGTTTACGTC CAGCCAAGCT AGCTTGGCTG CAGGTCGAGC GGCCGCGATC CGGAACCCTT AATATAACTT CGTATAATGT ATGCTATACG AAGTTATCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCTAAGAAAC CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TAACGCATTT AAGCATAAAC ACGCACTATG CCGTTCTTCT CATGTATATA TATATACAGG CAACACGCAG ATATAGGTGC GACGTGAACA GTGAGCTGTA TGTGCGCAGC TCGCGTTGCA TTTTCGGAAG CGCTCGTTTT CGGAAACGCT TTGAAGTTCC TATTCCGAAG TTCCTATTCT CTAGCTAGAA AGTATAGGAA CTTCAGAGCG CTTTTGAAAA CCAAAAGCGC TCTGAAGACG CACTTTCAAA AAACCAAAAA CGCACCGGAC TGTAACGAGC TACTAAAATA TTGCGAATAC CGCTTCCACA AACATTGCTC AAAAGTATCT CTTTGCTATA TATCTCTGTG CTATATCCCT ATATAACCTA CCCATCCACC TTTCGCTCCT TGAACTTGCA TCTAAACTCG ACCTCTACAT TTTTTATGTT TATCTCTAGT ATTACTCTTT AGACAAAAAA ATTGTAGTAA GAACTATTCA TAGAGTGAAT CGAAAACAAT ACGAAAATGT AAACATTTCC TATACGTAGT ATATAGAGAC AAAATAGAAG AAACCGTTCA TAATTTTCTG ACCAATGAAG AATCATCAAC GCTATCACTT TCTGTTCACA AAGTATGCGC AATCCACATC GGTATAGAAT ATAATCGGGG ATGCCTTTAT CTTGAAAAAA TGCACCCGCA GCTTCGCTAG TAATCAGTAA ACGCGGGAAG TGGAGTCAGG CTTTTTTTAT GGAAGAGAAA ATAGACACCA AAGTAGCCTT CTTCTAACCT TAACGGACCT ACAGTGCAAA AAGTTATCAA GAGACTGCAT TATAGAGCGC ACAAAGGAGA AAAAAAGTAA TCTAAGATGC TTTGTTAGAA AAATAGCGCT CTCGGGATGC ATTTTTGTAG AACAAAAAAG AAGTATAGAT TCTTTGTTGG TAAAATAGCG CTCTCGCGTT GCATTTCTGT TCTGTAAAAA TGCAGCTCAG ATTCTTTGTT TGAAAAATTA GCGCTCTCGC GTTGCATTTT TGTTTTACAA AAATGAAGCA CAGATTCTTC GTTGGTAAAA TAGCGCTTTC GCGTTGCATT TCTGTTCTGT AAAAATGCAG CTCAGATTCT TTGTTTGAAA AATTAGCGCT CTCGCGTTGC ATTTTTGTTC TACAAAATGA AGCACAGATG CTTCGTTGCT
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
