pJ23119-6×His-ntt1(UWE25)-rrnBT1
$70.00
In stock
SKU
PL24841
·
100μg liquid plasmid(Instantly):$91
| name | pJ23119-6×His-ntt1(UWE25)-rrnBT1 | another name | apJ23119-6×His-ntt1(UWE25)-rrnBT1 |
| Insert Size (bp) | 1542bp | Plasmid host | Escherichia coli |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | J23119 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pJ23119-MCS-rrnBT1 | 5′ sequencing primer | PUC57-F1 |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTGACAGCT AGCTCAGTCC TAGGTATAAT ACTAGTTTTC ACACAGGAAA CAGCTATGAC CATGTTACGC CAAGCTTATG CATCATCATC ATCATCACAT GTCGCAAGAT GCGAAACAAG ACTTTGGTAA GTGGAGGGCA TTCTTTTGGC CTGTACACGG TTATGAACTT AAAAAACTTC TTCCAATGTT TTTTATGTTC TTTTTTATTT CTTTCAATTA CACAATTTTA AGAGATACAA AAGACACTTT AATCGTGACC TCTGCAGGTG CGGAAGCCAT TCCATTTTTG AAGTCTTTTG GTGTTGTCCC TGCAGCTATC CTTTTTATGA TCATTTATGC CAAGCTTAGC AATACGCTAA GCAGAGAAAA TTTATTTTAC GTTACGCTTT TACCTTTTAT CATTTTCTTC GGCTTATTTG CTTTTGTCAT GTATCCAGCT AGAGAAGTTT TAATGCCTCA TGCTTCTGCT GAAGCTTTAA AAGCTTATTT ACCTGGCGGT TGGACCGGGC TTGCGGCGGC ATATGAAAAC TGGATGTATT CGATTTTTTA CATTTTAGCT GAACTTTGGG GAAGCGTTGT ATTATCTCTT CTTTTTTGGG GATTTGCTAA CCAAATTACC CGTGTAAATG AAGCTAAACG TTTTTATAGC CTTTTCGGTT TGGGTGCTAA TTTGGCACTT TTAGTTTCAG GTCCTGCTAT TGTATATGTA TCAGATATTC GCAAGCATTT ACCTGCAAAT GTTGATGCAT GGCAAATTTC TTTAAATTAT TTAATGGGTA TGGTCGTCAT TGCCGGATTA GCAATTTTAG CTATCTATTG GTGGATTAAT CGTGCAGTTT TAACAGATCC ACGTTTTTAT GATTTAAATC AAGAAAAAGC TCCTGGTAAA AAGAAAAAAG CTAAAATGTC TCTTGGAGAA AGCTTCAAAT TCCTCTTTAC CTCTAAGTAT ATCCTTTGTT TGGCGATATT GGTTATTGCT TACGGTATTT CTATTAACTT AGTAGAAATT ACATGGAAGA GCCTGGTGAA ACTTCAATAT CCTAATCCAA ATGATTACAG CACGTTCATG GGATGGTTCT CAACAATGAC GGGTGCCGTC ACAATTTTGA TGATGTTATT CGTTGGTGGT AACGTAATTC GTCACAAAGG TTGGGGATTT GCAGCTTTAA TTACTCCAGT TGTTTTATTA GTCACCGGGA TTGCTTTTTT CTCATTCGTG ATTTTTAAAG ATCACCTAGC TGGATATATT GCTGCCTTAG GAACTACTCC ACTATTCTTA GCTGTCATCT TTGGTGCGGC TCAAAATATT ATGAGCAAAT CTGCTAAATA CTCTTTATTT GATCCAACAA AAGAAATGGC TTATATTCCT TTAGATGATG AATCTAAAGT AAAAGGTAAA GCCGCAGTTG ACGTAGTAGG AGCTCGTTTA GGAAAATCTG GCGGATCTAT TATTCAAATG GGATTACTTG CATTTGGAAC GTTAGCAACT ATTACACCTT ATATCGGCGC GATTTTGATG GTTATTATTG CTGCCTGGAT TGTGGCTGCC CGTTCATTAA GCAAACAATT TACTCAATTG ACTGCTGAGC AGAATATTGA GAAAAATATT GATAAAACAT CGGAAATAGC TACTAGCTAA GAATTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA TTTTTCCAGG CATCAAATAA AACGAAAGTC TCAGTCGAAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG CTCTCCTGAG TAGGACAAAT CCGCCGGGAG CGGATTTGAA CGTTGCGAAG CAACGGCCCG GAGGGTGGCG GGCAGGACGC CCGCCATAAA CTGCCAGGCA TCAAATTAAG CAGAAGGCCA TCCTGACGGA TGGCCTTTTT GCGTTTCTAC AAACTCTGCC AGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
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