pAAV-Sox9(mouse)-Cre
$55.00
In stock
SKU
PL24826
-
100μg liquid plasmid(Instantly):$76
| name | pAAV-Sox9(mouse)-Cre | another name | apAAV-Sox9(mouse)-Cre |
| Insert Size (bp) | 468bp | Plasmid host | Mammary cell,Adeno-associated virus |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Red | |
| Promoter | Sox9 | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | pAAV.cTNT.iCre | 5′ sequencing primer | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTGCGCGCTC GCTCGCTCAC TGAGGCCGCC CGGGCAAAGC CCGGGCGTCG GGCGACCTTT GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT AGGGGTTCCT TGTAGTTAAT GATTAACCCG CCATGCTACT TATCTACCAG GGTAATGGGG ATCCTCTAGA ACTATAGCTA GAATTCTTCA TCGAAAAGTG GGGGTGGGGG GTTGTGGAGG GTCCTAGTCT AGACACGCTC GCGTGCACGC GCACACACAC ACACACACAC ACACATACAC ACACACACAC ACACACACAC ACACACACAC ACATCGGTTC ACACGGAGAC CGTTCCAAAA CTGTGACATT CCGAGAGTAG AGAGCAAACT TACACACTCG GACGTCCCGG GTCCCCCGCT TGCCCCGCGC CCCCCTCCAA GTCCCCTCAC CCCACCATCC ACCCTCTGGC TGAGCTCCCC TCCCTTCTCC TCCCCTCCTG TCTCGTCACC CAACCCGGAG CCACAATCCT CCCCAGCCCC CCTTCCAAAA TCCGGTCCAA TCAGCGACTT GCCAACACTG ATGACTCAAG AGCTAGCCGT GATTGGCCCG AGGTATCTAA CGTGAAGGAG GAGTATTTAT TAGAGACCCT GAGCTGGAAG TCGGAGAGCC GAGAGCGCTA GCATGGTGCC CAAGAAGAAG AGGAAAGTCT CCAACCTGCT GACTGTGCAC CAAAACCTGC CTGCCCTCCC TGTGGATGCC ACCTCTGATG AAGTCAGGAA GAACCTGATG GACATGTTCA GGGACAGGCA GGCCTTCTCT GAACACACCT GGAAGATGCT CCTGTCTGTG TGCAGATCCT GGGCTGCCTG GTGCAAGCTG AACAACAGGA AATGGTTCCC TGCTGAACCT GAGGATGTGA GGGACTACCT CCTGTACCTG CAAGCCAGAG GCCTGGCTGT GAAGACCATC CAACAGCACC TGGGCCAGCT CAACATGCTG CACAGGAGAT CTGGCCTGCC TCGCCCTTCT GACTCCAATG CTGTGTCCCT GGTGATGAGG AGAATCAGAA AGGAGAATGT GGATGCTGGG GAGAGAGCCA AGCAGGCCCT GGCCTTTGAA CGCACTGACT TTGACCAAGT CAGATCCCTG ATGGAGAACT CTGACAGATG CCAGGACATC AGGAACCTGG CCTTCCTGGG CATTGCCTAC AACACCCTGC TGCGCATTGC CGAAATTGCC AGAATCAGAG TGAAGGACAT CTCCCGCACC GATGGTGGGA GAATGCTGAT CCACATTGGC AGGACCAAGA CCCTGGTGTC CACAGCTGGT GTGGAGAAGG CCCTGTCCCT GGGGGTTACC AAGCTGGTGG AGAGATGGAT CTCTGTGTCT GGTGTGGCTG ATGACCCCAA CAACTACCTG TTCTGCCGGG TCAGAAAGAA TGGTGTGGCT GCCCCTTCTG CCACCTCCCA ACTGTCCACC CGGGCCCTGG AAGGGATCTT TGAGGCCACC CACCGCCTGA TCTATGGTGC CAAGGATGAC TCTGGGCAGA GATACCTGGC CTGGTCTGGC CACTCTGCCA GAGTGGGTGC TGCCAGGGAC ATGGCCAGGG CTGGTGTGTC CATCCCTGAA ATCATGCAGG CTGGTGGCTG GACCAATGTG AACATAGTGA TGAACTACAT CAGAAACCTG GACTCTGAGA CTGGGGCCAT GGTGAGGCTG CTCGAGGATG GGGACTGAGC GGCCGCGCCC CTCTCCCTCC CCCCCCCCTA ACGTTACTGG CCGAAGCCGC TTGGAATAAG GCCGGTGTGC GTTTGTCTAT ATGTTATTTT CCACCATATT GCCGTCTTTT GGCAATGTGA GGGCCCGGAA ACCTGGCCCT GTCTTCTTGA CGAGCATTCC TAGGGGTCTT TCCCCTCTCG CCAAAGGAAT GCAAGGTCTG TTGAATGTCG TGAAGGAAGC AGTTCCTCTG GAAGCTTCTT GAAGACAAAC AACGTCTGTA GCGACCCTTT GCAGGCAGCG GAACCCCCCA CCTGGCGACA GGTGCCTCTG CGGCCAAAAG CCACGTGTAT AAGATACACC TGCAAAGGCG GCACAACCCC AGTGCCACGT TGTGAGTTGG ATAGTTGTGG AAAGAGTCAA ATGGCTCTCC TCAAGCGTAT TCAACAAGGG GCTGAAGGAT GCCCAGAAGG TACCCCATTG TATGGGATCT GATCTGGGGC CTCGGTGCAC ATGCTTTACA TGTGTTTAGT CGAGGTTAAA AAAACGTCTA GGCCCCCCGA ACCACGGGGA CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC AACCCCATGG ACTAGTATGG GTTGCTGTTT CTCCAAGACC ATGGTGAGCA AGGGAGAGGA GGTCATCAAA GAGTTCATGC GCTTCAAGGT GCGCATGGAG GGCTCCATGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGCGGCC CCCTGCCCTT CGCCTGGGAC ATCCTGTCCC CCCAGTTCAT GTACGGCTCC AAGGCGTACG TGAAGCACCC CGCCGACATC CCCGATTACA AGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT GATGAACTTC GAGGACGGCG GTCTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG CACGCTGATC TACAAGGTGA AGATGCGCGG CACCAACTTC CCCCCCGACG GCCCCGTAAT GCAGAAGAAG ACCATGGGCT GGGAGGCCTC CACCGAGCGC CTGTACCCCC GCGACGGCGT GCTGAAGGGC GAGATCCACC AGGCCCTGAA GCTGAAGGAC GGCGGCCACT ACCTGGTGGA GTTCAAGACC ATCTACATGG CCAAGAAGCC CGTGCAACTG CCCGGCTACT ACTACGTGGA CACCAAGCTG GACATCACCT CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAGCG CTCCGAGGGC CGCCACCACC TGTTCCTGGG GCATGGCACC GGCAGCACCG GCAGCGGCAG CTCCGGCACC GCCTCCTCCG AGGACAACAA CATGGCCGTC ATCAAAGAGT TCATGCGCTT CAAGGTGCGC ATGGAGGGCT CCATGAACGG CCACGAGTTC GAGATCGAGG GCGAGGGCGA GGGCCGCCCC TACGAGGGCA CCCAGACCGC CAAGCTGAAG GTGACCAAGG GCGGCCCCCT GCCCTTCGCC TGGGACATCC TGTCCCCCCA GTTCATGTAC GGCTCCAAGG CGTACGTGAA GCACCCCGCC GACATCCCCG ATTACAAGAA GCTGTCCTTC CCCGAGGGCT TCAAGTGGGA GCGCGTGATG AACTTCGAGG ACGGCGGTCT GGTGACCGTG ACCCAGGACT CCTCCCTGCA GGACGGCACG CTGATCTACA AGGTGAAGAT GCGCGGCACC AACTTCCCCC CCGACGGCCC CGTAATGCAG AAGAAGACCA TGGGCTGGGA GGCCTCCACC GAGCGCCTGT ACCCCCGCGA CGGCGTGCTG AAGGGCGAGA TCCACCAGGC CCTGAAGCTG AAGGACGGCG GCCGCTACCT GGTGGAGTTC AAGACCATCT ACATGGCCAA GAAGCCCGTG CAACTGCCCG GCTACTACTA CGTGGACACC AAGCTGGACA TCACCTCCCA CAACGAGGAC TACACCATCG TGGAACAGTA CGAGCGCTCC GAGGGCCGCC ACCACCTGTT CCTGTACGGC ATGGACGAGC TGTACAAGGT CGACCCGGGC GGCCTCGAGG ACGGGGTGAA CTACGCCTGA GGATCCGATC TTTTTCCCTC TGCCAAAAAT TATGGGGACA TCATGAAGCC CCTTGAGCAT CTGACTTCTG GCTAATAAAG GAAATTTATT TTCATTGCAA TAGTGTGTTG GAATTTTTTG TGTCTCTCAC TCGGAAGCAA TTCGTTGATC TGAATTTCGA CCACCCATAA TACCCATTAC CCTGGTAGAT AAGTAGCATG GCGGGTTAAT CATTAACTAC AAGGAACCCC TAGTGATGGA GTTGGCCACT CCCTCTCTGC GCGCTCGCTC GCTCACTGAG GCCGGGCGAC CAAAGGTCGC CCGACGCCCG GGCTTTGCCC GGGCGGCCTC AGTGAGCGAG CGAGCGCGCA GCCTTAATTA ACCTAATTCA CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGGGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA ACGCTTACAA TTTAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAGA TTTAATTAAG GCCTTAATTA GG
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
