pCMV-mCherry-MYO1B(pig)-Neo
$99.00
In stock
SKU
PL24807
XM_021075966.1
100μg liquid plasmid(Instantly):$120
| name | pCMV-mCherry-MYO1B(pig)-Neo | another name | apCMV-mCherry-MYO1B(pig)-Neo |
| Insert Size (bp) | 3405bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Pig | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | Red |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pCMV-mCherry-2×Linker-MCS-Neo | 5′ sequencing primer | MCHERRY-F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGCT ACCGGTCGCC ACCATGGTGA GCAAGGGCGA GGAGGATAAC ATGGCCATCA TCAAGGAGTT CATGCGCTTC AAGGTGCACA TGGAGGGCTC CGTGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG GGCCGCCCCT ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG TGGCCCCCTG CCCTTCGCCT GGGACATCCT GTCCCCTCAG TTCATGTACG GCTCCAAGGC CTACGTGAAG CACCCCGCCG ACATCCCCGA CTACTTGAAG CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG CGCGTGATGA ACTTCGAGGA CGGCGGCGTG GTGACCGTGA CCCAGGACTC CTCCCTGCAG GACGGCGAGT TCATCTACAA GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC GTAATGCAGA AGAAGACCAT GGGCTGGGAG GCCTCCTCCG AGCGGATGTA CCCCGAGGAC GGCGCCCTGA AGGGCGAGAT CAAGCAGAGG CTGAAGCTGA AGGACGGCGG CCACTACGAC GCTGAGGTCA AGACCACCTA CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG CGCCTACAAC GTCAACATCA AGTTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT GGAACAGTAC GAACGCGCCG AGGGCCGCCA CTCCACCGGC GGCATGGACG AGCTGTACAA GGGTGGAGGC GGATCAGGAG GCGGTGGATC TCTCGAGGCC AAAATGGAGG TGAAGACGTC ACTTCTGGAC AATATGATTG GAGTTGGAGA TATGGTTCTT TTAGAACCTC TCAATGAGGA AACTGTCATC AACAACCTCA AGAAGCGCTT TGACCATAGC GAGATATATA CATACATTGG AAGTGTGGTT ATATCTGTTA ACCCATACCG GTCCTTGCCC ATTTATTCAC CAGAGAAAGT GGAAGATTAC AGGAACAGAA ATTTTTATGA ACTGAGCCCT CACATCTTTG CCCTGTCAGA TGAAGCATAC AGATCCCTAA GAGATCAAGA TAAAGACCAG TGTATTCTGA TTACTGGGGA AAGTGGAGCA GGAAAAACAG AGGCCAGCAA GCTTGTTATG TCCTACGTAG CGGCTGTTTG CGGAAAGGGA GCAGAAGTGA ATCAAGTCAA AGAACAGCTT TTACAGTCCA ACCCTGTCCT GGAAGCTTTT GGAAACGCCA AGACTGTAAG GAATGACAAC TCTTCTAGAT TTGGCAAATA TATGGATATT GAATTCGATT TTAAAGGCGA TCCGCTGGGA GGAGTGATAA GTAACTATCT TTTAGAGAAA TCTCGAGTCG TTAAGCAGCC AAGAGGTGAA AGAAACTTCC ACGTGTTCTA TCAGCTGCTT TCTGGTGGCT CTGAAGAGCT CCTCAATAAG CTTAAACTTG AGCGAGATTT CAGCAGATAC AACTACCTGA GTCTGGACTC AGCCAAAGTG AATGGAGTGG ATGATGCAGC AAATTTCAGA ACCGTTAGGA ATGCCATGCA GATTGTGGGT TTCATGGATC ATGAAGCTGA GTCTGTCTTG GAGGTGGTGG CGGCAGTGTT GAAGCTGGGG AACATTGAGT TCAAGCCTGA ATCTCGAGTG AATGGTTTAG ATGAAAGCAA GATCAAAGAT AAAAATGAGT TAAAAGAAAT TTGCGAGCTG ACTGGCATTG ATCAGTCAGT TCTAGAACGA GCCTTCAGCT TCCGAACAGT TGAGGCCAAG CAAGAGAAAG TTTCGACTAC ACTGAACGTG GCTCAGGCTT ATTATGCCCG TGATGCTCTG GCCAAAAACC TCTACAGCAG GTTGTTTTCA TGGTTGGTAA ATCGAATCAA TGAAAGCATT AAGGCACAAA CAAAAGTAAG AAAGAAGGTC ATGGGTGTTT TGGACATTTA TGGCTTCGAA ATTTTTGAGG ACAACAGCTT TGAGCAGTTC ATTATTAATT ATTGTAATGA AAAGCTGCAA CAAATCTTCA TTGAACTGAC TCTTAAAGAA GAGCAGGAGG AGTATATACG GGAGGGCATA GAATGGACTC ACATTGAATA CTTCAATAAT GCTATCATAT GTGACCTGAT AGAAAATAAC ACAAACGGAA TCCTGGCCAT GCTGGATGAG GAGTGCCTGA GGCCCGGCAC CGTCACCGAC GAGACCTTCT TGGAAAAGCT GAACCAAGTG TGCGCCACCC ACCAGCACTT TGAGAGCAGG ATGAGCAAGT GCTCCCGGTT CCTCAATGAC ACGTCCCTGC CTCACAGCTG CTTCAGAATC CAGCATTATG CTGGCAAGGT ACTGTACCAG GTGGAAGGAT TTGTGGATAA GAACAATGAC CTTCTCTACC GAGACCTGTC CCAAGCCATG TGGAAGGCCA GCCACGCCCT CATCAAGTCT TTGTTCCCTG AGGGGAACCC TGCCAAGATC AACCTGAAAA GGCCTCCTAC AGCTGGCTCC CAGTTCAAGG CATCTGTGGC CACTCTGATG AAAAACCTCC AGACCAAGAA TCCAAACTAC ATTAGGTGTA TCAAACCGAA TGATAAAAAA GCCGCACACA TCTTCAACGA GGGTCTGGTG TGTCATCAGA TCAGGTACCT GGGTCTTTTG GAGAATGTCC GCGTGAGGAG GGCGGGCTAT GCCTTCAGGC AGGCCTACGA ACCTTGCCTG GAAAGATACA AAATGCTGTG TAAACAAACA TGGCCTCATT GGAAAGGACC AGCAAGAGCT GGTGTGGAGG TTTTGTTTAA TGAATTGGAG ATTCCTGTGG AAGAATACTC CTTTGGTAGA TCAAAGATAT TCATTCGAAA CCCAAGAACA TTATTCAAGT TGGAAGACCT GAGGAAGCAG CGTCTTGAGG ACCTGGCCAC CCTCATTCAG AAGATTTATC GGGGGTGGAA ATGTCGCACA CACTTCCTCC TGATGAGAAA AAGCCAAATT GTGATCGCCG CCTGGTACAG GAGATACGCG CAACAAAAGA GGTATCAACA GATAAAGAGC TCGGCCTTGG TAATTCAGTC TTATATCCGG GGTTGGAAGG CCCGAAAAAT CCTGCGGGAA CTGAAGCATC AAAAGCGCTG TCAGGAAGCA GCCACCACCA TTGCAGCTTA TTGGCATGGT ACCCAGGCGC GAAGGGAACT GAGCCGGCTG AAGGCGGAGG CTAGGAATAA ACATGCTATT GCAGTTATTT GGGCTTACTG GCTTGGATCT AAGGCTCGAA GGGAGTTGAA ACGCTTGAAG GAAGAGGCTA GGCGTAAGCA TGCAGTTGCT GTCATTTGGG CTTACTGGCT TGGACTGAAG GTACGCAGGG AGTATAGGAA ATTCTTCAGA GCCAATGCTG GAAAGAAAAT TTATGAATTT ACGCTTCAGA GAATTATGCA AAAATACTTC TTGGAAATGA AAAATAAGGT GCCTTCCCTA TCTCCGATAG ACAAGAATTG GCCATCAAGA CCTTACCTAT TCTTGGATTC TACTCACAAG GAGCTAAAAA GGATTTTCCA CTTGTGGAGG TGTAAAAAAT ACAGGGACCA GTTCACAGAC CAACAGAAAC TTATTTACGA AGAGAAACTA GAAGCCAGTG AACTCTTCAA AGACAAGAAG GCTCTATACC CATCTAGTGT TGGGCAACCA TTCCAAGGGC CTTACCTGGA AATCAACAAG AATCCCAAGT ATAAGAAACT CAAGGATGCC ATTGAAGAAA AGATTATCAT TGCTGAAGTT GTGAACAAAA TTAACCGTGC TAATGGGAAG AGTACAAACC GGATTTTCCT CTTGACAAAT AATAATCTTC TTCTTGCTGA TCAAAAGTCT GGGCAGATCA AGTCAGAGGT TCCCCTGGTG GATGTAACCA AAGTATCAAT GAGCTCGCAA AATGATGGCT TCTTTGCCAT CCACCTCAAA GAGGGCTCTG AAGCAGCAAG TAAAGGAGAC TTTCTCTTCA GCAGTGATCA CCTGATTGAA ATGGCCACCA AGCTCTACCG GACGACTCTC AGCCAAACCA AACAGAAGCT CAATATTGAA ATTTCCGATG AGTTCCTGGT GCAGTTCAGA CAGGACAAAG TATGTGTGAA GTTTATTCAG GGCAACCAGA AAAATGGGAG TGTCCCAACG TGCAAGCGAA AAAACAACCG TCTCCTTGAA GTTGCTGTTC CTTAGGGATC CTCTAGAGGG CCCGTTTAAA CCCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA TGGCTTCTGA GGCGGAAAGA ACCAGCTGGG GCTCTAGGGG GTATCCCCAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTAAT TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCG ACGGATCGGG AGATCTCCCG ATCCCCTATG GT
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