pCS2-Htr6(mouse)-mCherry-GCaMP6s
$99.00
In stock
SKU
PL24287
-
100μg liquid plasmid(Instantly):$120
| name | pCS2-Htr6(mouse)-mCherry-GCaMP6s | another name | apCS2-Htr6(mouse)-mCherry-GCaMP6s |
| Insert Size (bp) | 3423bp | Plasmid host | Mammary cell,Fish |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Red | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pCS2+ | 5′ sequencing primer | MCHERRY-F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGT CGACCATAGC CAATTCAATA TGGCGTATAT GGACTCATGC CAATTCAATA TGGTGGATCT GGACCTGTGC CAATTCAATA TGGCGTATAT GGACTCGTGC CAATTCAATA TGGTGGATCT GGACCCCAGC CAATTCAATA TGGCGGACTT GGCACCATGC CAATTCAATA TGGCGGACTT GGCACTGTGC CAACTGGGGA GGGGTCTACT TGGCACGGTG CCAAGTTTGA GGAGGGGTCT TGGCCCTGTG CCAAGTCCGC CATATTGAAT TGGCATGGTG CCAATAATGG CGGCCATATT GGCTATATGC CAGGATCAAT ATATAGGCAA TATCCAATAT GGCCCTATGC CAATATGGCT ATTGGCCAGG TTCAATACTA TGTATTGGCC CTATGCCATA TAGTATTCCA TATATGGGTT TTCCTATTGA CGTAGATAGC CCCTCCCAAT GGGCGGTCCC ATATACCATA TATGGGGCTT CCTAATACCG CCCATAGCCA CTCCCCCATT GACGTCAATG GTCTCTATAT ATGGTCTTTC CTATTGACGT CATATGGGCG GTCCTATTGA CGTATATGGC GCCTCCCCCA TTGACGTCAA TTACGGTAAA TGGCCCGCCT GGCTCAATGC CCATTGACGT CAATAGGACC ACCCACCATT GACGTCAATG GGATGGCTCA TTGCCCATTC ATATCCGTTC TCACGCCCCC TATTGACGTC AATGACGGTA AATGGCCCAC TTGGCAGTAC ATCAATATCT ATTAATAGTA ACTTGGCAAG TACATTACTA TTGGAAGGAC GCCAGGGTAC ATTGGCAGTA CTCCCATTGA CGTCAATGGC GGTAAATGGC CCGCGATGGC TGCCAAGTAC ATCCCCATTG ACGTCAATGG GGAGGGGCAA TGACGCAAAT GGGCGTTCCA TTGACGTAAA TGGGCGGTAG GCGTGCCTAA TGGGAGGTCT ATATAAGCAA TGCTCGTTTA GGGAACCGCC ATTCTGCCTG GGGACGTCGG AGCAAGCTTG ATTTAGGTGA CACTATAGAA TACAAGCTAC TTGTTCTTTT TGCAGGATCC GCCACCATGG TTCCAGAGCC CGGCCCTGTC AACAGTAGCA CCCCAGCCTG GGGCCCCGGG CCACCGCCAG CCCCAGGGGG CAGCGGCTGG GTGGCCGCTG CGCTGTGCGT GGTCATCGTG CTGACGGCAG CCGCCAATTC GCTGCTGATC GCGCTCATCT GCACGCAGCC CGCGCTGCGC AACACGTCTA ACTTCTTCCT GGTGTCGCTC TTCACGTCGG ACCTGATGGT GGGATTGGTG GTGATGCCCC CAGCCATGCT GAACGCGCTG TATGGGCGCT GGGTGCTAGC TCGCGGCCTC TGTCTGCTTT GGACCGCCTT CGACGTGATG TGCTGCAGCG CCTCCATTCT CAACCTCTGC CTCATCAGCC TGGACCGCTA CCTGCTCATC CTCTCGCCGC TGCGCTACAA GCTGCGCATG ACAGCCCCGC GTGCCCTGGC GCTCATCCTG GGTGCCTGGA GCCTCGCCGC GCTGGCCTCC TTCCTGCCCC TCTTGCTGGG CTGGCATGAA CTGGGCAAAG CTCGAACATC TGCCCCGGGC CAGTGCCGCC TATTGGCCAG CCTGCCTTAT GTCCTCGTGG CGTCCGGCGT CACCTTTTTC CTGCCTTCGG GTGCCATCTG CTTCACCTAC TGCAGGATCT TGCTGGCTGC CCGCAAGCAG GCGGTGCAAG TGGCCTCGCT CACCACGGGC ACGGCTACGG CTGGCCAGGC CTTGGAAACC TTGCAGGTGC CCAGGACACC ACGCCCAGGG ATGGAGTCTG CTGACAGTAG GCGTCTGACC ACCAAGCATA GCAGGAAGGC CTTGAAGGCC AGCCTGACCC TCGGCATCCT GCTGAGCATG TTCTTTGTCA CCTGGCTGCC CTTCTTTGTG GCCAGCATAG CTCAGGCCGT ATGTGACTGC ATCTCTCCAG GTCTCTTCGA TGTCCTCACA TGGCTGGGAT ACTGTAATAG CACCATGAAC CCTATCATCT ATCCCCTCTT CATGCGGGAC TTCAAGAGGG CCCTGGGCAG GTTCGTGCCG TGTGTCCACT GTCCCCCGGA GCACCGGGCC AGCCCCGCCT CCCCCTCCAT GTGGACCTCT CACAGTGGTG CCAGGCCAGG CCTCAGCCTG CAGCAGGTGC TGCCCCTGCC TCTGCCACCC AACTCAGATT CAGACTCAGC TTCAGGGGGC ACCTCGGGCC TGCAGCTCAC AGCCCAGCTT TTGCTGCCTG GAGAGGCGAC CCGGGACCCC CCGCCACCCA CCAGGGCCCC TACTGTGGTC AACTTCTTCG TCACAGACTC TGTGGAGCCT GAGATACGGC AGCATCCACT TGGTTCCCCC ATGAACAGTG CTGGTGGTAG CGCAGGAGGA CCGGTCGCCA CCATGGTAAG CAAGGGCGAG GAGGATAACA TGGCCATCAT CAAGGAGTTC ATGCGCTTCA AGGTGCACAT GGAGGGCTCC GTGAACGGCC ACGAGTTCGA GATCGAGGGC GAGGGCGAGG GCCGCCCCTA CGAGGGCACC CAGACCGCCA AGCTGAAGGT GACCAAGGGT GGCCCCCTGC CCTTCGCCTG GGACATCCTG TCCCCTCAGT TCATGTACGG CTCCAAGGCC TACGTGAAGC ACCCCGCCGA CATCCCCGAC TACTTGAAGC TGTCCTTCCC CGAGGGCTTC AAGTGGGAGC GCGTGATGAA CTTCGAGGAC GGCGGCGTGG TGACCGTGAC CCAGGACTCC TCCCTGCAGG ACGGCGAGTT CATCTACAAG GTGAAGCTGC GCGGCACCAA CTTCCCCTCC GACGGCCCCG TAATGCAGAA GAAGACCATG GGCTGGGAGG CCTCCTCCGA GCGGATGTAC CCCGAGGACG GCGCCCTGAA GGGCGAGATC AAGCAGAGGC TGAAGCTGAA GGACGGCGGC CACTACGACG CTGAGGTCAA GACCACCTAC AAGGCCAAGA AGCCCGTGCA GCTGCCCGGC GCCTACAACG TCAACATCAA GTTGGACATC ACCTCCCACA ACGAGGACTA CACCATCGTG GAACAGTACG AACGCGCCGA GGGCCGCCAC TCCACCGGCG GCATGGACGA GCTGTACAAG GAATTCATGG GTTCTCATCA TCATCATCAT CATGGTATGG CTAGCATGAC TGGTGGACAG CAAATGGGTC GGGATCTGTA CGACGATGAC GATAAGGATC TCGCCACCAT GGTCGACTCA TCACGTCGTA AGTGGAATAA GACAGGTCAC GCAGTCAGAG CTATAGGTCG GCTGAGCTCA CTCGAGAACG TCTATATCAA GGCCGACAAG CAGAAGAACG GCATCAAGGC GAACTTCCAC ATCCGCCACA ACATCGAGGA CGGCGGCGTG CAGCTCGCCT ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCGT GCAGTCCAAA CTTTCGAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGGGCG GTACCGGAGG GAGCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGTGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACATCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACC TGCCGGACCA ACTGACTGAA GAGCAGATCG CAGAATTTAA AGAGGCTTTC TCCCTATTTG ACAAGGACGG GGATGGGACA ATAACAACCA AGGAGCTGGG GACGGTGATG CGGTCTCTGG GGCAGAACCC CACAGAAGCA GAGCTGCAGG ACATGATCAA TGAAGTAGAT GCCGACGGTG ACGGCACAAT CGACTTCCCT GAGTTCCTGA CAATGATGGC AAGAAAAATG AAATACAGGG ACACGGAAGA AGAAATTAGA GAAGCGTTCG GTGTGTTTGA TAAGGATGGC AATGGCTACA TCAGTGCAGC AGAGCTTCGC CACGTGATGA CAAACCTTGG AGAGAAGTTA ACAGATGAAG AGGTTGATGA AATGATCAGG GAAGCAGACA TCGATGGGGA TGGTCAGGTA AACTACGAAG AGTTTGTACA AATGATGACA GCGAAGTGAC TCGAGCCTCT AGAACTATAG TGAGTCGTAT TACGTAGATC CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAATTCGCG GCCGCGGCGC CAATGCATTG GGCCCGGTAC CCAGCTTTTG TTCCCTTTAG TGAGGGTTAA TTGCGCGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTAAATTG TAAGCGTTAA TATTTTGTTA AAATTCGCGT TAAATTTTTG TTAAATCAGC TCATTTTTTA ACCAATAGGC CGAAATCGGC AAAATCCCTT ATAAATCAAA AGAATAGACC GAGATAGGGT TGAGTGTTGT TCCAGTTTGG AACAAGAGTC CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA AACCGTCTAT CAGGGCGATG GCCCACTACG TGAACCATCA CCCTAATCAA GTTTTTTGGG GTCGAGGTGC CGTAAAGCAC TAAATCGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG CCGGCGAACG TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG GCAAGTGTAG CGGTCACGCT GCGCGTAACC ACCACACCCG CCGCGCTTAA TGCGCCGCTA CAGGGCGCGT CCCATTCGCC ATTCAGGCTG CG
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