pT3-U6-Lta4h(mouse)-shRNA1-EF1a-myr-AKT-HA
$55.00
In stock
SKU
PL24248
-
100μg liquid plasmid(Instantly):$76
| name | pT3-U6-Lta4h(mouse)-shRNA1-EF1a-myr-AKT-HA | another name | apT3-U6-Lta4h(mouse)-shRNA1-EF1a-myr-AKT-HA |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Interfere with silence | Fragment type | shRNA |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | U6 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pT3-myr-AKT-HA(mouse) | 5′ sequencing primer | U6-F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGCTCCGGTG CCCGTCAGTG GGCAGAGCGC ACATCGCCCA CAGTCCCCGA GAAGTTGGGG GGAGGGGTCG GCAATTGAAC CGGTGCCTAG AGAAGGTGGC GCGGGGTAAA CTGGGAAAGT GATGTCGTGT ACTGGCTCCG CCTTTTTCCC GAGGGTGGGG GAGAACCGTA TATAAGTGCA GTAGTCGCCG TGAACGTTCT TTTTCGCAAC GGGTTTGCCG CCAGAACACA GGTAAGTGCC GTGTGTGGTT CCCGCGGGCC TGGCCTCTTT ACGGGTTATG GCCCTTGCGT GCCTTGAATT ACTTCCACCT GGCTGCAGTA CGTGATTCTT GATCCCGAGC TTCGGGTTGG AAGTGGGTGG GAGAGTTCGA GGCCTTGCGC TTAAGGAGCC CCTTCGCCTC GTGCTTGAGT TGAGGCCTGG CCTGGGCGCT GGGGCCGCCG CGTGCGAATC TGGTGGCACC TTCGCGCCTG TCTCGCTGCT TTCGATAAGT CTCTAGCCAT TTAAAATTTT TGATGACCTG CTGCGACGCT TTTTTTCTGG CAAGATAGTC TTGTAAATGC GGGCCAAGAT CTGCACACTG GTATTTCGGT TTTTGGGGCC GCGGGCGGCG ACGGGGCCCG TGCGTCCCAG CGCACATGTT CGGCGAGGCG GGGCCTGCGA GCGCGGCCAC CGAGAATCGG ACGGGGGTAG TCTCAAGCTG GCCGGCCTGC TCTGGTGCCT GGCCTCGCGC CGCCGTGTAT CGCCCCGCCC TGGGCGGCAA GGCTGGCCCG GTCGGCACCA GTTGCGTGAG CGGAAAGATG GCCGCTTCCC GGCCCTGCTG CAGGGAGCTC AAAATGGAGG ACGCGGCGCT CGGGAGAGCG GGCGGGTGAG TCACCCACAC AAAGGAAAAG GGCCTTTCCG TCCTCAGCCG TCGCTTCATG TGACTCCACG GAGTACCGGG CGCCGTCCAG GCACCTCGAT TAGTTCTCGA GCTTTTGGAG TACGTCGTCT TTAGGTTGGG GGGAGGGGTT TTATGCGATG GAGTTTCCCC ACACTGAGTG GGTGGAGACT GAAGTTAGGC CAGCTTGGCA CTTGATGTAA TTCTCCTTGG AATTTGCCCT TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCAGACA GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAG GAATTAGCTT GGTACTAATA CGACTCACTA TAGGGAGACC CAAGCTGGCT AGGTAAGCTT GGTACCGAGC TCGGATCCAC TAGTCCAGTG TGGTGGAATT CTGCAGATAT CAACAAGTTT GTACAAAAAA GCAGGCTCCG CGGCCGCCCC CTTCACCATG GGGAGCAGCA AGAGCAAGCC CAAGTCTAGA ATGAACGACG TAGCCATTGT GAAGGAGGGC TGGCTGCACA AACGAGGGGA ATATATTAAA ACCTGGCGGC CACGCTACTT CCTCCTCAAG AACGATGGCA CCTTTATTGG CTACAAGGAA CGGCCTCAGG ATGTGGATCA GCGAGAGTCC CCACTCAACA ACTTCTCAGT GGCACAATGC CAGCTGATGA AGACAGAGCG GCCAAGGCCC AACACCTTTA TCATCCGCTG CCTGCAGTGG ACCACAGTCA TTGAGCGCAC CTTCCATGTG GAAACGCCTG AGGAGCGGGA AGAATGGGCC ACCGCCATTC AGACTGTGGC CGATGGACTC AAGAGGCAGG AAGAAGAGAC GATGGACTTC CGATCAGGCT CACCCAGTGA CAACTCAGGG GCTGAAGAGA TGGAGGTGTC CCTGGCCAAG CCCAAGCACC GTGTGACCAT GAACGAGTTT GAGTACCTGA AACTACTGGG CAAGGGCACC TTTGGGAAAG TGATTCTGGT GAAAGAGAAG GCCACAGGCC GCTACTATGC CATGAAGATC CTCAAGAAGG AGGTCATCGT CGCCAAGGAT GAGGTTGCCC ACACGCTTAC TGAGAACCGT GTCCTGCAGA ACTCTAGGCA TCCCTTCCTT ACGGCCCTCA AGTACTCATT CCAGACCCAC GACCGCCTCT GCTTTGTCAT GGAGTATGCC AACGGGGGCG AGCTCTTCTT CCACCTGTCT CGAGAGCGCG TGTTCTCCGA GGACCGGGCC CGCTTCTATG GTGCGGAGAT TGTGTCTGCC CTGGACTACT TGCACTCCGA GAAGAACGTG GTGTACCGGG ACCTGAAGCT GGAGAACCTC ATGCTGGACA AGGACGGGCA CATCAAGATA ACGGACTTCG GGCTGTGCAA GGAGGGGATC AAGGATGGTG CCACTATGAA GACATTCTGC GGAACGCCGG AGTACCTGGC CCCTGAGGTG CTGGAGGACA ACGACTACGG CCGTGCAGTG GACTGGTGGG GGCTGGGCGT GGTCATGTAT GAGATGATGT GTGGCCGCCT GCCCTTCTAC AACCAGGACC ACGAGAAGCT GTTCGAGCTG ATCCTCATGG AGGAGATCCG CTTCCCGCGC ACACTCGGCC CTGAGGCCAA GTCCCTGCTC TCCGGGCTGC TCAAGAAGGA CCCTACACAG AGGCTCGGTG GGGGCTCTGA GGATGCCAAG GAGATCATGC AGCACCGGTT CTTTGCCAAC ATCGTGTGGC AGGATGTGTA TGAGAAGAAG CTGAGCCCAC CTTTCAAGCC CCAGGTCACC TCTGAGACTG ACACCAGGTA TTTCGATGAG GAGTTCACAG CTCAGATGAT CACCATCACG CCGCCTGATC AAGATGACAG CATGGAGTGT GTGGACAGTG AGCGGAGGCC GCACTTCCCC CAGTTCTCCT ACTCAGCCAG TGGCACAGCC TACCCATACG ACGTCCCAGA CTACGCTTGA AAGGGTGGGC GCGCCGACCC AGCTTTCTTG TACAAAGTGG TTGATATCCA GCACAGTGGC GGCCGCTCGA GTCTAGAGGG CCCGCGGTTC GAAGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGCGTAC CGGTTAGTAA TGAGTTTAAA CCCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA TGGCTTCTGA GGCGGAAAGA ACCAGCTGGG GCTCTAGGGG GTATCCCCAC GCGCCCTGTA GCGGCGCATT AAATAACTTC GTATAATGTA TGCTATACGA AGTTATATCG TATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT CCACAGAATT CCTTGAAATA CATCCACAGG TACACCTCCA ATTGACTCAA ATGATGTCAA TTAGTCTATC AGAAGCTTCT AAAGCCATGA CATCATTTTC TGGAATTTTC CAAGCTGTTT AAAGGCACAG TCAACTTAGT GTATGTAAAC TTCTGACCCA CTGGAATTGT GATACAGTGA ATTATAAGTG AAATAATCTG TCTGTAAACA ATTGTTGGAA AAATGACTTG TGTCATGCAC AAAGTAGATG TCCTAACTGA CTTGCCAAAA CTATTGTTTG TTAACAAGAA ATTTGTGGAG TAGTTGAAAA ACGAGTTTTA ATGACTCCAA CTTAAGTGTA TGTAAACTTC CGACTTCAAC TGTAGGGTAC CGAGCTCGAA TTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG CCAAGCTTGC ATGCCTGCAG GTCGACTCTA GACCTATACA GTTGAAGTCG GAAGTTTACA TACACTTAAG TTGGAGTCAT TAAAACTCGT TTTTCAACTA CTCCACAAAT TTCTTGTTAA CAAACAATAG TTTTGGCAAG TCAGTTAGGA CATCTACTTT GTGCATGACA CAAGTCATTT TTCCAACAAT TGTTTACAGA CAGATTATTT CACTTATAAT TCACTGTATC ACAATTCCAG TGGGTCAGAA GTTTACATAC ACTAAGTTGA CTGTGCCTTT AAACAGCTTG GAAAATTCCA GAAAATGATG TCATGGCTTT AGAAGCTTCT GATAGACTAA TTGACATCAT TTGAGTCAAT TGGAGGTGTA CCTGTGGATG TATTTCAAGG GGATCCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGCG GCCGCTAGCG AGGGCCTATT TCCCATGATT CCTTCATATT TGCATATACG ATACAAGGCT GTTAGAGAGA TAATTAGAAT TAATTTGACT GTAAACACAA AGATATTAGT ACAAAATACG TGACGTAGAA AGTAATAATT TCTTGGGTAG TTTGCAGTTT TAAAATTATG TTTTAAAATG GACTATCATA TGCTTACCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAATTCGGCT TTGGTTGTTG GAGCTTTATT CAAGAGATAA AGCTCCAACA ACCAAAGCTT TTTTGCTAGC TCGAGATCTT AATTAAACTA GTATCGATAT AACTTCGTAT AATGTATGCT ATACGAAGTT ATATGTACGG GCCAGATATA CGCGTTGACA TTGATTATTG ACTAGGCTTT TGCAAAAAGC TTTGCAAAGA TGGATAAAGT TTTAAACAGA GAGGAATCTT TGCAGCTAAT GGACCTTCTA GGTCTTGAAA GGAGTGGGAA TT
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